LOCUS       sequence-403834-       13481 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  induction of phase-separated condensate mediated by BRD4dN-IDR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13481)
  AUTHORS   Kim YJ, Lee M Jr, Lee YT, Jing J, Sanders JT, Botten GA, He L, Lyu 
            J, Zhang Y, Mettlen M, Ly P, Zhou Y, Xu J
  TITLE     Light-activated macromolecular phase separation modulates 
            transcription by reconfiguring chromatin interactions.
  JOURNAL   Sci Adv. 2023 Mar 31;9(13):eadg1123. doi: 10.1126/sciadv.adg1123. 
            Epub 2023 Mar 31.
  PUBMED    37000871
REFERENCE   2  (bases 1 to 13481)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-03-31"; volume: "9(13):eadg1123. doi"; pages: " 
            10.1126/sciadv.adg1123. Epub 2023 Mar 31"
COMMENT     Sequence Label: dCas9-CRY2Clust-BRD4-Delta-N
FEATURES             Location/Qualifiers
     source          1..13481
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3223..3243
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3613..3734
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3650..3669)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3704..3723
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3741..4196)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3878..3899)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4090..4109
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4223..4327
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4329..4686
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4329..4520
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4352..4372)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4537..4672
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4721..5515
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4721..5515
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4775..4794)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5385..5404
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5703..5722)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5747..5794
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6123..6711
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6612..6631
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        6886..7189
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7190..7393
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7344..7364
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             8259..8276
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             10161..10868
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(10306..10324)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     10574..10593
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             complement(12448..12465)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             12498..12542
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             12573..12593
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(12594..12617,12618..12620,12621..13481,1..1626,
                     1627..1629,1630..3213)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 gacgccatcc tgctgtctga cattctccgc gtgaacactg aaatcaccaa ggcccctctt
       61 tcagcttcaa tgattaagcg gtatgatgag caccaccagg acctgaccct gcttaaggca
      121 ctcgtccggc agcagcttcc ggagaagtac aaggaaatct tctttgacca gtcaaagaat
      181 ggatacgccg gctacatcga cggaggtgcc tcccaagagg aattttataa gtttatcaaa
      241 cctatccttg agaagatgga cggcaccgaa gagctcctcg tgaaactgaa tcgggaggat
      301 ctgctgcgga agcagcgcac tttcgacaat gggagcattc cccaccagat ccatcttggg
      361 gagcttcacg ccatccttcg gcgccaagag gacttctacc cctttcttaa ggacaacagg
      421 gagaagattg agaaaattct cactttccgc atcccctact acgtgggacc cctcgccaga
      481 ggaaatagcc ggtttgcttg gatgaccaga aagtcagaag aaactatcac tccctggaac
      541 ttcgaagagg tggtggacaa gggagccagc gctcagtcat tcatcgaacg gatgactaac
      601 ttcgataaga acctccccaa tgagaaggtc ctgccgaaac attccctgct ctacgagtac
      661 tttaccgtgt acaacgagct gaccaaggtg aaatatgtca ccgaagggat gaggaagccc
      721 gcattcctgt caggcgaaca aaagaaggca attgtggacc ttctgttcaa gaccaataga
      781 aaggtgaccg tgaagcagct gaaggaggac tatttcaaga aaattgaatg cttcgactct
      841 gtggagatta gcggggtcga agatcggttc aacgcaagcc tgggtaccta ccatgatctg
      901 cttaagatca tcaaggacaa ggattttctg gacaatgagg agaacgagga catccttgag
      961 gacattgtcc tgactctcac tctgttcgag gaccgggaaa tgatcgagga gaggcttaag
     1021 acctacgccc atctgttcga cgataaagtg atgaagcaac ttaaacggag aagatatacc
     1081 ggatggggac gccttagccg caaactcatc aacggaatcc gggacaaaca gagcggaaag
     1141 accattcttg atttccttaa gagcgacgga ttcgctaatc gcaacttcat gcaacttatc
     1201 catgatgatt ccctgacctt taaggaggac atccagaagg cccaagtgtc tggacaaggt
     1261 gactcactgc acgagcatat cgcaaatctg gctggttcac ccgctattaa gaagggtatt
     1321 ctccagaccg tgaaagtcgt ggacgagctg gtcaaggtga tgggtcgcca taaaccagag
     1381 aacattgtca tcgagatggc cagggaaaac cagactaccc agaagggaca gaagaacagc
     1441 agggagcgga tgaaaagaat tgaggaaggg attaaggagc tcgggtcaca gatccttaaa
     1501 gagcacccgg tggaaaacac ccagcttcag aatgagaagc tctatctgta ctaccttcaa
     1561 aatggacgcg atatgtatgt ggaccaagag cttgatatca acaggctctc agactacgac
     1621 gtggacgcca tcgtccctca gagcttcctc aaagacgact caattgacaa taaggtgctg
     1681 actcgctcag acaagaaccg gggaaagtca gataacgtgc cctcagagga agtcgtgaaa
     1741 aagatgaaga actattggcg ccagcttctg aacgcaaagc tgatcactca gcggaagttc
     1801 gacaatctca ctaaggctga gaggggcgga ctgagcgaac tggacaaagc aggattcatt
     1861 aaacggcaac ttgtggagac tcggcagatt actaaacatg tcgcccaaat ccttgactca
     1921 cgcatgaata ccaagtacga cgaaaacgac aaacttatcc gcgaggtgaa ggtgattacc
     1981 ctgaagtcca agctggtcag cgatttcaga aaggactttc aattctacaa agtgcgggag
     2041 atcaataact atcatcatgc tcatgacgca tatctgaatg ccgtggtggg aaccgccctg
     2101 atcaagaagt acccaaagct ggaaagcgag ttcgtgtacg gagactacaa ggtctacgac
     2161 gtgcgcaaga tgattgccaa atctgagcag gagatcggaa aggccaccgc aaagtacttc
     2221 ttctacagca acatcatgaa tttcttcaag accgaaatca cccttgcaaa cggtgagatc
     2281 cggaagaggc cgctcatcga gactaatggg gagactggcg aaatcgtgtg ggacaagggc
     2341 agagatttcg ctaccgtgcg caaagtgctt tctatgcctc aagtgaacat cgtgaagaaa
     2401 accgaggtgc aaaccggagg cttttctaag gaatcaatcc tccccaagcg caactccgac
     2461 aagctcattg caaggaagaa ggattgggac cctaagaagt acggcggatt cgattcacca
     2521 actgtggctt attctgtcct ggtcgtggct aaggtggaaa aaggaaagtc taagaagctc
     2581 aagagcgtga aggaactgct gggtatcacc attatggagc gcagctcctt cgagaagaac
     2641 ccaattgact ttctcgaagc caaaggttac aaggaagtca agaaggacct tatcatcaag
     2701 ctcccaaagt atagcctgtt cgaactggag aatgggcgga agcggatgct cgcctccgct
     2761 ggcgaacttc agaagggtaa tgagctggct ctcccctcca agtacgtgaa tttcctctac
     2821 cttgcaagcc attacgagaa gctgaagggg agccccgagg acaacgagca aaagcaactg
     2881 tttgtggagc agcataagca ttatctggac gagatcattg agcagatttc cgagttttct
     2941 aaacgcgtca ttctcgctga tgccaacctc gataaagtcc ttagcgcata caataagcac
     3001 agagacaaac caattcggga gcaggctgag aatatcatcc acctgttcac cctcaccaat
     3061 cttggtgccc ctgccgcatt caagtacttc gacaccacca tcgaccggaa acgctatacc
     3121 tccaccaaag aagtgctgga cgccaccctc atccaccaga gcatcaccgg actttacgaa
     3181 actcggattg acctctcaca gctcggaggg gatgagggag ctcccaagaa aaagcgcaag
     3241 gtaggtagtt ccgccggaaa ggccggtgaa ggtgaaatcc ctgcccctct tgctggtacc
     3301 gtttctaaga tactggtaaa agaaggtgac actgttaaag ctggtcaaac agttctggtg
     3361 ctggaggcta tgaaaatgga gacagaaatt aacgctccta ctgacggaaa agttgaaaag
     3421 gtgttagtta aggaaagaga tgctgttcaa ggtggtcaag gtctaatcaa gatcggcgtt
     3481 tagagatcca ccggatctag ataactgatc ataatcagcc ataccacatt tgtagaggtt
     3541 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     3601 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     3661 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     3721 atcaatgtat cttaacgcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     3781 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     3841 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     3901 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     3961 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     4021 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     4081 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     4141 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg
     4201 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     4261 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     4321 gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
     4381 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4441 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4501 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     4561 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     4621 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     4681 tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     4741 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     4801 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     4861 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
     4921 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     4981 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     5041 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     5101 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     5161 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     5221 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac
     5281 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     5341 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     5401 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     5461 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     5521 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
     5581 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     5641 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta
     5701 actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga
     5761 cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc
     5821 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc
     5881 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg
     5941 gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt
     6001 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     6061 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6121 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6181 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6241 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     6301 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6361 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6421 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6481 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     6541 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     6601 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     6661 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     6721 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     6781 gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcattagtt attaatagta
     6841 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     6901 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     6961 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     7021 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     7081 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     7141 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     7201 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     7261 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     7321 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     7381 tataagcaga gctggtttag tgaaccgtca gatccgctag cgaattcatg gaggagccag
     7441 tggtggccgt gtcctccccg gcagtgcccc ctcccaccaa ggttgtggcc ccgccctcat
     7501 ccagcgacag cagcagcgat agctcctcgg acagtgacag ttcgactgat gactctgagg
     7561 aggagcgagc ccagcggctg gctgagctcc aggagcagct caaagccgtg cacgagcagc
     7621 ttgcagccct ctctcagccc cagcagaaca aaccaaagaa aaaggagaaa gacaagaagg
     7681 aaaagaaaaa agaaaagcac aaaaggaaag aggaagtgga agagaataaa aaaagcaaag
     7741 ccaaggaacc tcctcctaaa aagacgaaga aaaataatag cagcaacagc aatgtgagca
     7801 agaaggagcc agcgcccatg aagagcaagc cccctcccac gtatgagtcg gaggaagagg
     7861 acaagtgcaa gcctatgtcc tatgaggaga agcggcagct cagcttggac atcaacaagc
     7921 tccccggcga gaagctgggc cgcgtggtgc acatcatcca gtcacgggag ccctccctga
     7981 agaattccaa ccccgacgag attgaaatcg actttgagac cctgaagccg tccacactgc
     8041 gtgagctgga gcgctatgtc acctcctgtt tgcggaagaa aaggaaacct caagctgaga
     8101 aagttgatgt gattgccggc tcctccaaga tgaagggctt ctcgtcctca gagtcggaga
     8161 gctccagtga gtccagctcc tctgacagcg aagactccga aacagagatg gctccgaagt
     8221 caaaaaagaa ggggcacccc gggagggagc agaagaagca ccatcatcac caccatcagc
     8281 agatgcagca ggccccggct cctgtgcccc agcagccgcc cccgcctccc cagcagcccc
     8341 caccgcctcc acctccgcag cagcaacagc agccgccacc cccgcctccc ccaccctcca
     8401 tgccgcagca ggcagccccg gcgatgaagt cctcgccccc acccttcatt gccacccagg
     8461 tgcccgtcct ggagccccag ctcccaggca gcgtctttga ccccatcggc cacttcaccc
     8521 agcccatcct gcacctgccg cagcctgagc tgccccctca cctgccccag ccgcctgagc
     8581 acagcactcc accccatctc aaccagcacg cagtggtctc tcctccagct ttgcacaacg
     8641 cactacccca gcagccatca cggcccagca accgagccgc tgccctgcct cccaagcccg
     8701 cccggccccc agccgtgtca ccagccttga cccaaacacc cctgctccca cagcccccca
     8761 tggcccaacc cccccaagtg ctgctggagg atgaagagcc acctgcccca cccctcacct
     8821 ccatgcagat gcagctgtac ctgcagcagc tgcagaaggt gcagccccct acgccgctac
     8881 tcccttccgt gaaggtgcag tcccagcccc caccccccct gccgccccca ccccacccct
     8941 ctgtgcagca gcagctgcag cagcagccgc caccaccccc accaccccag ccccagcctc
     9001 caccccagca gcagcatcag ccccctccac ggcccgtgca cttgcagccc atgcagtttt
     9061 ccacccacat ccaacagccc ccgccacccc agggccagca gcccccccat ccgcccccag
     9121 gccagcagcc acccccgccg cagcctgcca agcctcagca agtcatccag caccaccatt
     9181 caccccggca ccacaagtcg gacccctact caaccggtca cctccgcgaa gccccctccc
     9241 cgcttatgat acattccccc cagatgtcac agttccagag cctgacccac cagtctccac
     9301 cccagcaaaa cgtccagcct aagaaacagg agctgcgtgc tgcctccgtg gtccagcccc
     9361 agcccctcgt ggtggtgaag gaggagaaga tccactcacc catcatccgc agcgagccct
     9421 tcagcccctc gctgcggccg gagcccccca agcacccgga gagcatcaag gcccccgtcc
     9481 acctgcccca gcggccggaa atgaagcctg tggatgtcgg gaggcctgtg atccggcccc
     9541 cagagcagaa cgcaccgcca ccaggggccc ctgacaagga caaacagaaa caggagccga
     9601 agactccagt tgcgcccaaa aaggacctga aaatcaagaa catgggctcc tgggccagcc
     9661 tagtgcagaa gcatccgacc accccctcct ccacagccaa gtcatccagc gacagcttcg
     9721 agcagttccg ccgcgccgct cgggagaaag aggagcgtga gaaggccctg aaggctcagg
     9781 ccgagcacgc tgagaaggag aaggagcggc tgcggcagga gcgcatgagg agccgagagg
     9841 acgaggatgc gctggagcag gcccggcggg cccatgagga ggcacgtcgg cgccaggagc
     9901 agcagcagca gcagcgccag gagcaacagc agcagcagca acagcaagca gctgcggtgg
     9961 ctgccgccgc caccccacag gcccagagct cccagcccca gtccatgctg gaccagcaga
    10021 gggagttggc ccggaagcgg gagcaggagc gaagacgccg ggaagccatg gcagctacca
    10081 ttgacatgaa tttccagagt gatctattgt caatatttga agaaaatctt ttctgggcgc
    10141 acactagtgc tagcggtgga atggtgagca agggcgagga ggataacatg gccatcatca
    10201 aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga
    10261 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
    10321 ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct
    10381 ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg
    10441 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc
    10501 aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact
    10561 tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc
    10621 ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg
    10681 acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc
    10741 tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca
    10801 ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc
    10861 tgtacaaggg tagcggacca ccggtcgcca ccatgaagat ggacaaaaag accatcgtct
    10921 ggtttcggag agatttgaga atagaagata atcccgcgct cgccgccgcg gcccacgagg
    10981 gttccgtctt ccccgttttc atttggtgtc ctgaagaaga aggccagttt tatcccggaa
    11041 gggcctctag gtggtggatg aagcaaagtc tggcccatct tagccagtca ctgaaagcac
    11101 tgggcagtga tcttaccctg atcaagacac acaataccat ctctgccatc ctcgactgca
    11161 tcagggtgac cggcgcaacg aaagtcgtgt ttaaccacct gtacgatcca gttagtctgg
    11221 tgcgcgacca cactgtgaag gagaagctgg tggaacgggg gatcagtgtg cagagctaca
    11281 acggggacct tctgtacgag ccatgggaga tctattgcga gaaagggaaa ccgttcacct
    11341 ccttcaacag ttactggaag aaatgtttgg atatgtcaat agagtccgtt atgttgcccc
    11401 ctccctggag actgatgccg attactgctg ctgcagaggc catctgggcc tgctccatcg
    11461 aggaactcgg tctggaaaat gaagcagaaa agccaagcaa tgcacttctc actagagcct
    11521 ggagccccgg ctggtctaat gccgacaagc tgcttaacga gttcatcgaa aaacaactga
    11581 ttgactacgc gaagaactcc aagaaagtgg taggtaactc aactagcttg ctctctccat
    11641 atctccattt tggcgagatt tctgtccgcc atgtatttca gtgcgctcgg atgaaacaga
    11701 ttatctgggc tcgcgataaa aacagcgaag gcgaagaaag cgccgatctg ttcctgcgag
    11761 ggatcggact tcgggaatac tcccggtata tatgtttcaa ctttccattc acacacgagc
    11821 agagtctgtt gtcccacctc aggttcttcc cctgggacgc cgatgtcgac aaattcaagg
    11881 catggagaca gggaaggaca gggtacccac tcgtggatgc tggcatgaga gagctctggg
    11941 ctacaggctg gatgcacaac cgcatccggg taatcgtgtc ctcatttgct gtcaagtttc
    12001 tgctcctgcc ttggaaatgg ggaatgaagt acttttggga tacccttctc gacgccgact
    12061 tggagtgtga cattctggga tggcaatata ttagcgggtc aattcctgac ggccatgagt
    12121 tggacaggtt ggacaatccg gccttgcagg gagctaagta tgatcccgaa ggagagtata
    12181 ttcgacagtg gctccccgag ctggcccgac ttcctacgga gtggattcac catccttggg
    12241 acgcaccact gacagtgctc aaggcaagcg gggtggagct gggcaccaat tacgctaagc
    12301 ctatagttga tatagataca gcacgcgagc tgctggctaa agcgatctct cgcactcggg
    12361 aggcgcagat tatgatcggt gctgccgcaa gggaccctcc tgatcttgac aatgtgtaca
    12421 gtgggggagg cggagggggt ggttcaggtg gtggtggtgg tggtggttcg ggtggtggcg
    12481 gagggggagg ctccgggggc ctgaacgaca tcttcgaggc tcagaaaatc gaatggcacg
    12541 aaggcgcgcc gagctcgagg atccttgtct gccccaaaaa gaagaggaaa gtggacaaga
    12601 agtattctat cggactggcc atcgggacta atagcgtcgg gtgggccgtg atcactgacg
    12661 agtacaaggt gccctctaag aagttcaagg tgctcgggaa caccgaccgg cattccatca
    12721 agaaaaatct gatcggagct ctcctctttg attcagggga gaccgctgaa gcaacccgcc
    12781 tcaagcggac tgctagacgg cggtacacca ggaggaagaa ccggatttgt taccttcaag
    12841 agatattctc caacgaaatg gcaaaggtcg acgacagctt cttccatagg ctggaagaat
    12901 cattcctcgt ggaagaggat aagaagcatg aacggcatcc catcttcggt aatatcgtcg
    12961 acgaggtggc ctatcacgag aaatacccaa ccatctacca tcttcgcaaa aagctggtgg
    13021 actcaaccga caaggcagac ctccggctta tctacctggc cctggcccac atgatcaagt
    13081 tcagaggcca cttcctgatc gagggcgacc tcaatcctga caatagcgat gtggataaac
    13141 tgttcatcca gctggtgcag acttacaacc agctctttga agagaacccc atcaatgcaa
    13201 gcggagtcga tgccaaggcc attctgtcag cccggctgtc aaagagccgc agacttgaga
    13261 atcttatcgc tcagctgccg ggtgaaaaga aaaatggact gttcgggaac ctgattgctc
    13321 tttcacttgg gctgactccc aatttcaagt ctaatttcga cctggcagag gatgccaagc
    13381 tgcaactgtc caaggacacc tatgatgacg atctcgacaa cctcctggcc cagatcggtg
    13441 accaatacgc cgaccttttc cttgctgcta agaatctttc t
//
