LOCUS       sequence-403864-       12740 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produce lentivirus encoding Nluc-EPAC sensor in neuronal cell type.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12740)
  AUTHORS   Park JC, Luebbers A, Dao M, Semeano A, Nguyen AM, Papakonstantinou 
            MP, Broselid S, Yano H, Martemyanov KA, Garcia-Marcos M
  TITLE     Fine-tuning GPCR-mediated neuromodulation by biasing signaling 
            through different G protein subunits.
  JOURNAL   Mol Cell. 2023 Jul 20;83(14):2540-2558.e12. doi: 
            10.1016/j.molcel.2023.06.006. Epub 2023 Jun 29.
  PUBMED    37390816
REFERENCE   2  (bases 1 to 12740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.06.006"; journalName: "Mol Cell"; date: 
            "2023-07-20- 20"; volume: "83"; issue: "14"; pages: "2540-2558.e12"
COMMENT     Sequence Label: pLenti-hSynapsin-Nluc-EPAC-VV
FEATURES             Location/Qualifiers
     source          1..12740
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2632..3079
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      3115..3124
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3121..3633
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             5857..6591
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     CDS             join(6649..6651,6652..6654,6655..7365)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    7432..8020
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7485..7505)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7903..7914)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8023..8039)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8024..8040)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8545..8725
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8751..8768)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8757..8981
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9027..9455
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9114..9133)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9324..9345
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9464..9484)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9469..9798
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9649..9784
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9711..9730
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9846..9893
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9912..10286
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10416..10537
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10453..10472)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10507..10526
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10586..10602)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10586..10602)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10599..10621)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10610..10626
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10634..10640,10641..10658,10659..10664))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10679..10700
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10817..10834)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10988..11576)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11068..11087)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11747..12538,12539..12607))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12370..12389
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12608..12712)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagcacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta agtgtctaga ctgcagaggg ccctgcgtat gagtgcaagt
     2641 gggttttagg accaggatga ggcggggtgg gggtgcctac ctgacgaccg accccgaccc
     2701 actggacaag cacccaaccc ccattcccca aattgcgcat cccctatcag agagggggag
     2761 gggaaacagg atgcggcgag gcgcgtgcgc actgccagct tcagcaccgc ggacagtgcc
     2821 ttcgcccccg cctggcggcg cgcgccaccg ccgcctcagc actgaaggcg cgctgacgtc
     2881 actcgccggt cccccgcaaa ctccccttcc cggccacctt ggtcgcgtcc gcgccgccgc
     2941 cggcccagcc ggaccgcacc acgcgaggcg cgagataggg gggcacgggc gcgaccatct
     3001 gcgctgcggc gccggcgact cagcgctgcc tcagtctgcg gtgggcagcg gaggagtcgt
     3061 gtcgtgcctg agagcgcagt cgagaaaccg gctagaggat ccttcgaaac cggtgccacc
     3121 atggtcttca cactcgaaga tttcgttggg gactggcgac agacagccgg ctacaacctg
     3181 gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta
     3241 actccgatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga catccatgtc
     3301 atcatcccgt atgaaggtct gagcggcgac caaatgggcc agatcgaaaa aatttttaag
     3361 gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc tgcactatgg cacactggta
     3421 atcgacgggg ttacgccgaa catgatcgac tatttcggac ggccgtatga aggcatcgcc
     3481 gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa caaaattatc
     3541 gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc gagtaaccat caacggagtg
     3601 accggctggc ggctgtgcga acgcattctg gcgcgatatc atcagcccgt gggaactcat
     3661 gagatggagg aggagttggc cgaagctgtg gccctgctct cccagcgggg gcctgacgcc
     3721 ctgctcactg tggcacttcg aaagccccca ggtcagcgca cggatgaaga gctggacctc
     3781 atctttgagg agctgctgca catcaaggct gtggcccacc tctccaactc ggtgaagcga
     3841 gaattagcgg ctgttctgct ctttgaacca cacagcaagg cagggaccgt gttgttcagc
     3901 cagggggaca agggcacttc gtggtacatt atctggaagg gatctgtcaa cgtggtgacc
     3961 catggcaagg ggctggtgac caccctgcat gagggagatg attttggaca gctggctctg
     4021 gtgaatgatg caccccgggc agccaccatc atcctgcgag aagacaactg tcatttcctg
     4081 cgtgtggaca agcaggactt caaccgtatc atcaaggatg tggaggcaaa gaccatgcgg
     4141 ctggaagaac atggcaaagt ggtgctggtg ctggagagag cctctcaggg cgccggccct
     4201 tcccgacccc caaccccagg caggaaccgg tatacagtga tgtctggcac cccagagaag
     4261 atcctagagc ttctgttgga ggccatggga ccagattcca gtgctcatga cccaacagag
     4321 acattcctca gcgacttcct cctgacccac agggtcttca tgcccagcgc ccaactctgc
     4381 gctgcccttc tgcaccactt ccatgtggag cctgcgggtg gcagcgagca ggagcgcagc
     4441 acctacgtct gcaacaagag gcagcagatc ttgcggctgg tcagccagtg ggtggccctg
     4501 tatggctcca tgctccacac tgaccctgtg gccaccagct tcctccagaa actctcagac
     4561 ctggtgggca gggacacccg actcagcaac ctgctgaggg agcagtggcc agagaggcgg
     4621 cgatgccaca ggttggagaa tggctgtggg aatgcatctc ctcagatgaa ggcccggaac
     4681 ttgcctgttt ggctccccaa ccaggacgag ccccttcctg gcagcagctg tgccatccaa
     4741 gttggggata aagtccccta tgacatctgc cggccagacc actcagtgtt gaccctgcag
     4801 ctgcctgtga cagcctccgt gagagaggtg atggcagcgt tggcccagga ggatggctgg
     4861 accaaggggc aggtgctggt gaaggtcaat tctgcaggtg atgccattgg cctgcagcca
     4921 gatgcccgtg gtgtggccac atctctgggg ctcaatgagc gtctctttgt tgtcaaccca
     4981 caggaagcgc atgagctgat cccacaccct gaccagctgg ggcccactgt gggctctgct
     5041 gaggggctgg acctggtgag tgccaaggac ctggcaggcc agctgacgga ccacgactgg
     5101 agcctcttca acagtatcca ccaggtggag ctgatccact atgtgctggg cccccagcat
     5161 ctgcgggatg tcaccaccgc caacctggag cgcttcatgc gccgcttcaa tgagctgcag
     5221 tactgggtgg ccaccgagct gtgtctctgc cccgtgcccg gcccccgggc ccagctgctc
     5281 aggaagttca ttaagctggc ggcccacctc aaggagcaga agaatctcaa ttccttcttt
     5341 gccgtcatgt ttggcctcag caactcggcc atcagccgcc tagcccacac ctgggagcgg
     5401 ctgccccaca aagtccggaa gctgtactcc gccctcgaga ggctgctgga tccctcatgg
     5461 aaccaccggg tataccgact ggccctcgcc aagctctccc ctcctgtcat ccccttcatg
     5521 ccccttcttc tcaaagacat ggccgccatt catgagggaa accacacact agtggagaat
     5581 ctcatcaact ttgagaagat gagaatgatg gccagagccg cgcggatgct gcaccactgc
     5641 cgaagccaca accctgtgcc tctctcacca ctcagaagcc gagtttccca cctccacgag
     5701 gacagccagg tggcgaggat ttccacatgc tcggagcagt ccctgagcac ccggagtcca
     5761 gccagcacct gggcttatgt ccagcagctg aaggtcattg acaaccagcg ggaactctcc
     5821 cgcctctccc gagagctgga gccagctagc gagctcatgg acggcggcgt gcagctcgcc
     5881 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     5941 tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     6001 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagggt
     6061 ggcagcggtg gcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     6121 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     6181 gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg
     6241 ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc ccgctacccc
     6301 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     6361 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     6421 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     6481 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac
     6541 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga gccgaatttt
     6601 gtcttcctga tcggcgccgc aggaatactc ttcgtatcta gcgagctcat ggtgagcaag
     6661 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     6721 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     6781 ctgaagctga tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccact
     6841 ctgggctacg gcctrcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
     6901 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     6961 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     7021 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     7081 aactacaaca gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc
     7141 aacttcaaga tccgccacaa catcgaggac ggcggcgtgc agctcgccga ccactaccag
     7201 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac
     7261 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     7321 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagccgaa ttccctcgag
     7381 gttaacgcgt tagcgcctct aatctaggaa ttcgatatca agcttatcga taatcaacct
     7441 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     7501 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     7561 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     7621 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     7681 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     7741 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     7801 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     7861 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     7921 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     7981 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
     8041 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
     8101 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
     8161 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     8221 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac
     8281 cacacacaag gctacttccc tgattggcag aactacacac cagggccagg gatcagatat
     8341 ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa
     8401 gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac
     8461 ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc
     8521 cgagagctgc atccggactg tactgggtct ctctggttag accagatctg agcctgggag
     8581 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     8641 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     8701 tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt
     8761 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     8821 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     8881 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     8941 agacaatagc aggcatgctg gggatgcggc gggctctatg gcttctgagg cggaaagaac
     9001 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     9061 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     9121 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     9181 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     9241 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     9301 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     9361 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     9421 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     9481 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     9541 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     9601 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     9661 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     9721 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     9781 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag
     9841 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     9901 ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
     9961 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
    10021 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
    10081 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
    10141 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
    10201 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
    10261 acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct
    10321 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
    10381 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
    10441 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    10501 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
    10561 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
    10621 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
    10681 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
    10741 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
    10801 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
    10861 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
    10921 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
    10981 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
    11041 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
    11101 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
    11161 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
    11221 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
    11281 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    11341 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    11401 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
    11461 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    11521 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    11581 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    11641 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    11701 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    11761 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    11821 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    11881 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    11941 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    12001 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    12061 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    12121 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    12181 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    12241 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    12301 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    12361 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    12421 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    12481 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    12541 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    12601 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    12661 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    12721 cccgaaaagt gccacctgac
//
