LOCUS       sequence-403945-       12418 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RNA editing in plants. Plasmid contains extradiol-LIC 
            cassette-dADARcd-35S-bar.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12418)
  AUTHORS   Zhou G, Niu R, Zhou Y, Luo M, Peng Y, Wang H, Wang Z, Xu G
  TITLE     Proximity editing to identify RNAs in phase-separated RNA binding 
            protein condensates.
  JOURNAL   Cell Discov. 2021 Aug 24;7(1):72. doi: 10.1038/s41421-021-00288-9.
  PUBMED    34426573
REFERENCE   2  (bases 1 to 12418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-08-24"; volume: "7(1):72. doi"; pages: " 
            10.1038/s41421-021-00288-9"
COMMENT     Sequence Label: pZG160
FEATURES             Location/Qualifiers
     source          1..12418
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        216..262
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             291..542
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
     CDS             558..791
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             801..1736
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             849..1742
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     promoter        2300..2479
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2332..2515
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2597..3148
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      3150..3402
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        3723..3769
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(5619..5636)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5619..5635)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5628..5650)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    5857..5881
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6402..7193
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7439..8027
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7928..7947
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8181..8198
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8213..8353
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8279..8298)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8439..8461
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8697..8891
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8957..10030)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10459..11088)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc taagcttgca tgcctgcagg atatcgtgga tccaagcttg
      121 ccacgtgccg ccacgtgccg ccacgtgccg ccacgtgcct ctagaggatc catctccact
      181 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
      241 agttcatttc atttggagag gacacgctgg gatccccaat tccgggcgga atgaaagcgt
      301 taacggccag gcaacaagag gtgtttgatc tcatccgtga tcacatcagc cagacaggta
      361 tgccgccgac gcgtgcggaa atcgcgcagc gtttggggtt ccgttcccca aacgcggctg
      421 aagaacatct gaaggcgctg gcacgcaaag gcgttattga aattgtttcc ggcgcatcac
      481 gcgggattcg tctgttgcag gaagaggaag aagggttgcc gctggtaggt cgtgtggctg
      541 ccggtgaacc gtcgagcgcc cccccgaccg atgtcagcct gggggacgag ctccacttag
      601 acggcgagga cgtggcgatg gcgcatgccg acgcgctaga cgatttcgat ctggacatgt
      661 tgggggacgg ggattccccg ggtccgggat ttacccccca cgactccgcc ccctacggcg
      721 ctctggatat ggccgacttc gagtttgagc agatgtttac cgatgccctt ggaattgacg
      781 agtacggtgg ggatccgtct gctggagaca tgagagctgc caacctttgg ccaagcccgc
      841 tcatgatcaa acgctctaag aagaacagcc tggccttgtc cctgacggcc gaccagatgg
      901 tcagtgcctt gttggatgct gagcccccca tactctattc cgagtatgat cctaccagac
      961 ccttcagtga agcttcgatg atgggcttac tgaccaacct ggcagacagg gagctggttc
     1021 acatgatcaa ctgggcgaag agggtgccag gctttgtgga tttgaccctc catgatcagg
     1081 tccaccttct agaatgtgcc tggctagaga tcctgatgat tggtctcgtc tggcgctcca
     1141 tggagcaccc agggaagcta ctgtttgctc ctaacttgct cttggacagg aaccagggaa
     1201 aatgtgtaga gggcatggtg gagatcttcg acatgctgct ggctacatca tctcggttcc
     1261 gcatgatgaa tctgcaggga gaggagtttg tgtgcctcaa atctattatt ttgcttaatt
     1321 ctggagtgta cacatttctg tccagcaccc tgaagtctct ggaagagaag gaccatatcc
     1381 accgagtcct ggacaagatc acagacactt tgatccacct gatggccaag gcaggcctga
     1441 ccctgcagca gcagcaccag cggctggccc agctcctcct catcctctcc cacatcaggc
     1501 acatgagtaa caaaggcatg gagcatctgt acagcatgaa gtgcaagaac gtggtgcccc
     1561 tctatgacct gctgctggag atgctggacg cccaccgcct acatgcgccc actagccgtg
     1621 gaggggcatc cgtggaggag acggaccaaa gccacttggc cactgcgggc tctacttcat
     1681 cgcattcctt gcaaaagtat tacatcacgg gggaggcaga gggtttccct gccacagtct
     1741 gagagctccc tggcggaatt cccagagatg ttagctgaaa tcatcactaa tcagatacca
     1801 aaatattcaa atggaaatat caaaaagctt ctgtttcatc aaaaatgact cgacctaact
     1861 gagtaagcta gcttgttcga gtattatggc attgggaaaa ctgtttttct tgtaccattt
     1921 gttgtgcttg taatttactg tgttttttat tcggttttcg ctatcgaact gtgaaatgga
     1981 aatggatgga gaagagttaa tgaatgatat ggtccttttg ttcattctca aattaatatt
     2041 atttgttttt tctcttattt gttgtgtgtt gaatttgaaa ttataagaga tatgcaaaca
     2101 ttttgttttg agtaaaaatg tgtcaaatcg tggcctctaa tgaccgaagt taatatgagg
     2161 agtaaaacac tagatcccca aacaagcttg aaactgaagg cgggaaacga caatctgatc
     2221 atgagcggag aattaaggga gtcacgttat gacccccgcc gatgacgcgg gacaagccgt
     2281 tttacgtttg gaactgacag aaccgcaacg ttgaaggagc cactcagccg cgggtttctg
     2341 gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa aagtcgccta
     2401 aggtcactat cagctagcaa atatttcttg tcaaaaatgc tccactgacg ttccataaat
     2461 tcccctcggt atccaattag agtctcatat tcactctcaa tccaaataat ctgcaccgga
     2521 tccgctagag gatctcgacc tgcaagatcc cggggggcaa tgagatatga aaaagcctga
     2581 actcaccgcg acgtctatga gcccagaacg acgcccggcc gacatccgcc gtgccaccga
     2641 ggcggacatg ccggcggtct gcaccatcgt caaccactac atcgagacaa gcacggtcaa
     2701 cttccgtacc gagccgcagg aaccgcagga gtggacggac gacctcgtcc gtctgcggga
     2761 gcgctatccc tggctcgtcg ccgaggtgga cggcgaggtc gccggcatcg cctacgcggg
     2821 accctggaag gcacgcaacg cctacgactg gacggccgag tcgaccgtgt acgtctcccc
     2881 ccgccaccag cggacgggac tgggctccac gctctacacc cacctgctga agtccctgga
     2941 ggcacagggc ttcaagagcg tggtcgctgt catcgggctg cccaacgacc cgagcgtgcg
     3001 catgcacgag gcgctcggat atgccccccg cggcatgctg cgggcggccg gcttcaagca
     3061 cgggaactgg catgacgtgg gtttctggca gctggacttc agcctgccgg taccgccccg
     3121 tccggtcctg cccgtcaccg agatctaacg atcgttcaaa catttggcaa taaagtttct
     3181 taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg
     3241 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
     3301 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
     3361 aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg atcccccctc
     3421 gacagcttgc atgccgcttg ggctgcaggt cgaggctaaa aaactaatcg cattatcatc
     3481 ccctcgacgt actgtacata taaccactgg ttttatatac agcagtactg tacatataac
     3541 cactggtttt atatacagca gtcgacgtac tgtacatata accactggtt ttatatacag
     3601 cagtactgta catataacca ctggttttat atacagcagt cgaggtaaga ttagatatgg
     3661 atatgtatat ggatatgtat atggtggtaa tgccatgtaa tatgctcgac tctaggatct
     3721 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac acgctgaagc
     3781 tagtcgactc tagcctcgag taccttcgac gacaagaccg ggcccggctc ttctcctcac
     3841 tcgaccagat ctcgtacgcg tcccggggcg gtggctcatc tggcggaggt ggatcctact
     3901 ctcctatggt cgtaccgcaa aaaaatgtgc ctctccctat agacgataaa tctagctcca
     3961 tggagcttcc gcagatccac gcagacacta tcggtcgatt ggtgctggaa aagttcatgg
     4021 aggttattaa aggtcaggag gcttatagcc gtcgaaaagt tttggctgga atcgtaatga
     4081 ccgaaaacat gaacttctgt gaggcaaaag tcatttcagt gtccacgggc accaagtgtg
     4141 tgtccggcga gcatatgtcc gtaaacggtg cggtactcaa tgacagccac gccgagatag
     4201 tgtcacgtcg ttgtctcctc aagtatctct atgcccaact ggatcttcaa tgcaaccagg
     4261 cgaccgctta tcagagcata tttgtgcgta atactgacgg tcagtatccc tataaactga
     4321 agtctggggt ccattttcat ctctatataa acacggcgcc ctgcggcgat gcccgtattt
     4381 tttcacctca cgagaatgac acgggagtag ataaacatcc caaccgaaaa gcaagagggc
     4441 agctccgaac caaaatcgaa tccggccagg gcacaatccc agtgaaatct agcgacggca
     4501 ttcaaacttg ggacggcgta ctacaaggcc agcgtttgct tacgatgtcc tgctccgata
     4561 aaattgctag gtggaacatc gtcggtatcc agggctcctt gctctcttca attatagagc
     4621 ccgtctatct gcattcaatt gtcttagggt cactcttgca tcctgaacat atgtaccgag
     4681 ccgtttgtgg gagaatagaa aagagtatcc agggtctacc cccaccttac catttgaaca
     4741 aacccagact ggcattggtc acatccgcag aaccacgtaa ccaagccaag gccccgaatt
     4801 ttggaataaa ctggactata ggtgacacgg aactagaggt tgtgaactct ctcacaggca
     4861 gaaccatagg agggcaagtc agccgtataa cgaagcaggc ttttttcgta aagtatggtt
     4921 tcttgatggc taatttgccc ggaatcctcg tccgaaaggt aacgaccgat tacggtcaga
     4981 caaaagcgaa tgtaaaggat taccagatcg cgaagttgga attgttctca gccttcaaga
     5041 gggaggatct tggatcatgg ctaaagaaac caatagagca ggacgagttt ggattggctg
     5101 aatgaactag tcgatccagg cctcccagct ttcgtccgta tcatcggttt cgacaacgtt
     5161 cgtcaagttc aatgcatcag tttcattgcc cacacaccag aatcctacta agtttgagta
     5221 ttatggcatt ggaaaagctg ttttcttcta tcatttgttc tgcttgtaat ttactgtgtt
     5281 ctttcagttt ttgttttcgg acatcaaaat gcaaatggat ggataagagt taataaatga
     5341 tatggtcctt ttgttcattc tcaaattatt attatctgtt gtttttactt taatgggttg
     5401 aatttaagta agaaaggaac taacagtgtg atattaaggt gcaatgttag acatataaaa
     5461 cagtctttca cctctctttg gttatgtctt gaattggttt gtttcttcac ttatctgtgt
     5521 aatcaagttt actatgagtc tatgatcaag taattatgca atcaagttaa gtacagtata
     5581 ggctttttgt gtcgaggggg taccgagtcg aggaattcac tggccgtcgt tttacaacgt
     5641 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     5701 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     5761 ctgaatggcg ggtaccgagc tcgaattcaa ttcggcgtta attcagtaca ttaaaaacgt
     5821 ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat atatcctgcc
     5881 accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac tcgatacagg
     5941 cagcccatca gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc agactttgct
     6001 catgttaccg atgctattcg gaagaacggc aactaagctg ccgggtttga aacacggatg
     6061 atctcgcgga gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcaa
     6121 ttcgggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc
     6181 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     6241 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat
     6301 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     6361 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgggggaa gcggtgatcg
     6421 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     6481 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg
     6541 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     6601 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     6661 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     6721 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     6781 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     6841 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     6901 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     6961 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     7021 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     7081 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     7141 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taatgtctag
     7201 ctagaaattc gttcaagccg acgccgcttc gcggcgcggc ttaactcaag cgttagatgc
     7261 actaagcaca taattgctca cagccaaact atcaggtcaa gtctgctttt attattttta
     7321 agcgtgcata ataagcccta cacaaattgg gagatatatc atgcatgacc aaaatccctt
     7381 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     7441 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     7501 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     7561 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     7621 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     7681 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     7741 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     7801 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     7861 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     7921 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     7981 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     8041 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     8101 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     8161 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
     8221 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
     8281 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     8341 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     8401 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     8461 gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg cgccggcggt
     8521 cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt aggccagcca
     8581 tttttgagcg gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt agggagcgca
     8641 gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt gcgctggcca gacagttatg
     8701 cacaggccag gcgggtttta agagttttaa taagttttaa agagttttag gcggaaaaat
     8761 cgcctttttt ctcttttata tcagtcactt acatgtgtga ccggttccca atgtacggct
     8821 ttgggttccc aatgtacggg ttccggttcc caatgtacgg ctttgggttc ccaatgtacg
     8881 tgctatccac aggaaagaga ccttttcgac ctttttcccc tgctagggca atttgcccta
     8941 gcatctgctc cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc
     9001 gcatgactag gatcgggcca gcctgccccg cctcctcctt caaatcgtac tccggcaggt
     9061 catttgaccc gatcagcttg cgcacggtga aacagaactt cttgaactct ccggcgctgc
     9121 cactgcgttc gtagatcgtc ttgaacaacc atctggcttc tgccttgcct gcggcgcggc
     9181 gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa
     9241 ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg gtacatccaa tcagctagct
     9301 cgatctcgat gtactccggc cgcccggttt cgctctttac gatcttgtag cggctaatca
     9361 aggcttcacc ctcggatacc gtcaccaggc ggccgttctt ggccttcttc gtacgctgca
     9421 tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac caggtcgtct ttctgctttc
     9481 cgccatcggc tcgccggcag aacttgagta cgtccgcaac gtgtggacgg aacacgcggc
     9541 cgggcttgtc tcccttccct tcccggtatc ggttcatgga ttcggttaga tgggaaaccg
     9601 ccatcagtac caggtcgtaa tcccacacac tggccatgcc ggccggccct gcggaaacct
     9661 ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg tcacgcttcg
     9721 acagacggaa aacggccacg tccatgatgc tgcgactatc gcgggtgccc acgtcataga
     9781 gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg cggcttccta atcgacggcg
     9841 caccggctgc cggcggttgc cgggattctt tgcggattcg atcagcggcc gcttgccacg
     9901 attcaccggg gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc gcggccttca
     9961 acttctccac caggtcatca cccagcgccg cgccgatttg taccgggccg gatggtttgc
    10021 gaccgctcac gccgattcct cgggcttggg ggttccagtg ccattgcagg gccggcagac
    10081 aacccagccg cttacgcctg gccaaccgcc cgttcctcca cacatggggc attccacggc
    10141 gtcggtgcct ggttgttctt gattttccat gccgcctcct ttagccgcta aaattcatct
    10201 actcatttat tcatttgctc atttactctg gtagctgcgc gatgtattca gatagcagct
    10261 cggtaatggt cttgccttgg cgtaccgcgt acatcttcag cttggtgtga tcctccgccg
    10321 gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc caggctggcc aacgttgcag
    10381 ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt ttgctcattt
    10441 tctctttacc tcattaactc aaatgagttt tgatttaatt tcagcggcca gcgcctggac
    10501 ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg cggcggcagt
    10561 gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga atgggcagct cgtacccggc
    10621 cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa
    10681 ggccgcttgt agccttccat ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc
    10741 ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga atcagcacga agtcggctgc
    10801 cttgatcgcg gacacagcca agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc
    10861 gcgccggccg atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt
    10921 tagcggttga tcttcccgca cggccgccca atcgcgggca ctgccctggg gatcggaatc
    10981 gactaacaga acatcggccc cggcgagttg cagggcgcgg gctagatggg ttgcgatggt
    11041 cgtcttgcct gacccgcctt tctggttaag tacagcgata accttcatgc gttccccttg
    11101 cgtatttgtt tatttactca tcgcatcata tacgcagcga ccgcatgacg caagctgttt
    11161 tactcaaata cacatcacct ttttagacgg cggcgctcgg tttcttcagc ggccaagctg
    11221 gccggccagg ccgccagctt ggcatcagac aaaccggcca ggatttcatg cagccgcacg
    11281 gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc cgcctcgatc
    11341 tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat gcttgttcct
    11401 cttggcgttc attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg tcctcacgga
    11461 aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt
    11521 acagggtcga gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg cggccttcct
    11581 ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg cgggggccaa
    11641 acttcacgcc tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg
    11701 aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac catgcggccg gccggcgtgg
    11761 tggtgtcggc ccacggctct gccaggctac gcaggcccgc gccggcctcc tggatgcgct
    11821 cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg gtcactgtca
    11881 caacgtcgcc agggcgtagg tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg
    11941 tgccggtgat cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt
    12001 ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata gcccagcagg ccagcggcgg
    12061 cgctcttgtt catggcgtaa tgtctccggt tctagtcgca agtattctac tttatgcgac
    12121 taaaacacgc gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt
    12181 aggacttgtg cgacatgtcg ttttcagaag acggctgcac tgaacgtcag aagccgactg
    12241 cactatagca gcggaggggt tggatcaaag tactttaaag tactttaaag tactttaaag
    12301 tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga cgaacggata
    12361 aaccttttca cgccctttta aatatccgat tattctaata aacgctcttt tctcttag
//
