LOCUS       sequence-403953-        2860 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5S rDNA used as a probe for chromosome FISH in nematode.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Tabara H, Mitani S, Mochizuki M, Kohara Y, Nagata K
  TITLE     A small RNA system ensures accurate homologous pairing and unpaired 
            silencing of meiotic chromosomes.
  JOURNAL   EMBO J. 2023 Jun 1;42(11):e105002. doi: 10.15252/embj.2020105002. 
            Epub 2023 Apr 20.
  PUBMED    37078421
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2023-06-1"; volume: "42(11):e105002. doi"; pages: " 
            10.15252/embj.2020105002. Epub 2023 Apr 20"
COMMENT     Sequence Label: pHIT346
FEATURES             Location/Qualifiers
     source          1..2860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     58..77
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        58..76
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        954..1058
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1059..1127,1128..1919)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1277..1296)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2090..2678
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2579..2598
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2832..2849
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctgtaa
       61 tacgactcac tatagggtcg acttgaaggc gcgtctctgc gcctaacacc aacctacagc
      121 agacagcgag cgaggtctac gatcggcaac ccccctctcc acacaacaca cagccagcca
      181 gtagcactga cgaaggggta gaggcctagt cgggtggtgg ggggtttgta actgacatgg
      241 cacttgtcca tagacgctta cgaccatatc acgttgaatg cacgccatcc cgtccgatct
      301 ggcaagttaa gcaacgttga gtccagttag tacttggatc ggagacggcc tgggaatcct
      361 ggatgttgta agctttttgc attttttttt tagaatgact aaaaaaattt gtttttgttg
      421 caacatcata tgaattagaa acgattttaa aataattttc aaaaaatttt tccccagcca
      481 aaatgacact tctggagagt cgtattttcg agggtaatta agagactgct atttttttta
      541 ggtgtgtgat gtgttggact ttattgcgtg tacctaatga gtgaatattg attatactgg
      601 atgtgtcggg tcgatttttg gaacatgaaa aatttaggtt cttttgaaaa tttttgagaa
      661 caattgcatt tttcagttga ggagcgcacg gcacccatac atgagaatct ataatttttt
      721 tgcatcacat agactatttc catatatgta tgtaccagta gcaagaatga aaagcacttt
      781 tgaagagtgt agatgagctg gagggcaaca tcatatttag atcggggatc ccgtccccca
      841 atcaatatca tcaccttccc acatctccac atattttcga atggaccgtt ctccaacatg
      901 gatgaaccga ttttcgaatt cagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
      961 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     1021 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     1081 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     1141 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     1201 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     1261 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     1321 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     1381 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     1441 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     1501 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     1561 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     1621 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     1681 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     1741 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     1801 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     1861 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     1921 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     1981 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     2041 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     2101 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2161 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     2221 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2281 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2341 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2401 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2461 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     2521 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     2581 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     2641 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     2701 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     2761 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     2821 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
//
