LOCUS       sequence-403958-       16803 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express MSI1-ZF108 in Arabidopsis target FWA gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16803)
  AUTHORS   Wang M, Zhong Z, Gallego-Bartolome J, Li Z, Feng S, Kuo HY, Kan RL, 
            Lam H, Richey JC, Tang L, Zhou J, Liu M, Jami-Alahmadi Y, 
            Wohlschlegel J, Jacobsen SE
  TITLE     A gene silencing screen uncovers diverse tools for targeted gene 
            repression in Arabidopsis.
  JOURNAL   Nat Plants. 2023 Mar 6. doi: 10.1038/s41477-023-01362-8.
  PUBMED    36879017
REFERENCE   2  (bases 1 to 16803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2023 Mar 6. doi: 10.1038/s41477-023-01362-8."
COMMENT     Sequence Label: pEG302-MSI1-ZF
FEATURES             Location/Qualifiers
     source          1..16803
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..915
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(841..860)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1001..1023
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      1259..1453
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1519..2592)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3021..3650)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4951..4975
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     5163..5185
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5177..5194
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5178..5194
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    5217..5391
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(5394..6359)
                     /codon_start=1
                     /gene="birA"
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             complement(5397..6359)
                     /codon_start=1
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     terminator      complement(7820..8527)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     CDS             complement(8607..8651)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             complement(9177..9200)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(9207..9278)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     protein_bind    9337..9361
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    9408..9419
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     protein_bind    complement(12775..12799)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        12851..13231
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             13237..13788
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      13798..14050
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    14136..14160
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             14585..15379
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
      841 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
      901 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
      961 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     1021 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg
     1081 gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc
     1141 catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg
     1201 cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta
     1261 tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa
     1321 atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg
     1381 ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta
     1441 cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc
     1501 tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta
     1561 gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag
     1621 gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct
     1681 gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg
     1741 gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg
     1801 aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag
     1861 ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat
     1921 caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg
     1981 catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt
     2041 tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg
     2101 gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac
     2161 cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac
     2221 ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt
     2281 cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata
     2341 gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg
     2401 cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca
     2461 cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt
     2521 caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt
     2581 gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
     2641 gcaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
     2701 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
     2761 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
     2821 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
     2881 cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc
     2941 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
     3001 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
     3061 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
     3121 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
     3181 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
     3241 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
     3301 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
     3361 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
     3421 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
     3481 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
     3541 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
     3601 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
     3661 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
     3721 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
     3781 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
     3841 cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
     3901 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
     3961 ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg
     4021 gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg
     4081 gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc
     4141 ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc
     4201 aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag
     4261 ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt
     4321 ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg
     4381 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
     4441 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
     4501 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
     4561 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
     4621 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
     4681 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
     4741 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
     4801 tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg
     4861 ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg
     4921 attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac
     4981 tgatagttta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
     5041 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     5101 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     5161 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcagggatc
     5221 tggattttag tactggattt tggttttagg aattagaaat tttattgata gaagtatttt
     5281 acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg aaaccctata
     5341 ggaaccctaa ttcccttatc tgggaactac tcacacatta ttatggagaa agcttatttt
     5401 tctgcactac gcagggatat ttcaccgccc atccagggtt ttattattcc atcctgctca
     5461 agtaataaag ccccctgttt gtctattccg cgtgaaatgc caaatatttc tttatcacca
     5521 atgataagtt tcactgggcg attaataaaa ttatccagct tttcccagcg cgacagataa
     5581 ggtgccaatc cttcttgttc gaagagttcc aacgcagcac gtaattcacg tattagcatg
     5641 gccgccaacg tattacgatc gagattgatc cccgcttcct gcagcgtgat ccacccctga
     5701 ttaacgacac tctcttcaac acggcgcatt gccatgttga tcccggctcc aatgactatt
     5761 tgcgccgcat cgccagtttt gccagtcagc tccaccagaa tgcctgccag cttgcgatcc
     5821 tgcagataga ggtcattagg ccatttaaca cgaactttat ctgcacccag cttgcgtaat
     5881 acttccgcca tcacgatacc gataaccaga cttaaaccaa tcgccgccgc cgggccttgt
     5941 tccagacgcc agaacatcga caaatataag tttgcgccaa aaggcgaaaa ccatttccga
     6001 ccccggcgac cacggccagc ctgctggtat tctgcaatgc aagcatcgcc cgatttaagc
     6061 tctccgatac gatcaagaag gtactgattc gtggagtcaa tcactggcag cacggctaca
     6121 ctaccgccat ccagctgacc caatatctgt ttagcattaa gtaactggat aggctcaggc
     6181 aggctgtatc ctttacccgg aacggtaaag acatcaacgc cccagtcacg cagtgtctga
     6241 atgtgtttat taatagccgc ccggctcatt cccagcgttt cacccaactg ctcgccagag
     6301 tgaaattcac cgttcgctaa cagggcaatc aatttcagtg gcacggtgtt atccttcatc
     6361 tcgaggtcac acacgattgg ttgaatatca tcagcctcag ccatttttta tgagctgcaa
     6421 acacacaaaa agagttcaat acagtcaaat aaaccctgat tgaatcagaa atagctttct
     6481 gttcaacgta cgacactaca aatcctaaag tttcgaagaa tctatacaca aacttcatct
     6541 aaccttaaac agtgttcaga ttcaatctaa cattgacagt gttcagattc aatctaactt
     6601 caacagttca gatttcaatt cacatataca aatcatttcc caggtaacat gaacatggat
     6661 ctctccatca aggtcaagcc aaattctggt agctataatc gagctaactg atcggtcaac
     6721 ttagatcgat tctagtaaaa ccaaaagatt tagtggaggt tcacagatcc aaattaaacg
     6781 atatccagat ctcaaggaaa ttgagaaaac aagatcgaga tccagcaaat aaagtagatc
     6841 cagaaagttc ctattacctt ggaaagaaag agcggaagaa gatgagattg aggaagattc
     6901 aaaacggaga atgaatctcc tggattatct cttactttct ctcttagtct tcttccttgt
     6961 tcttctctgt caagtcgccg gagattcaaa acggctgatg aaagtgagga ggacaacgag
     7021 acaattcaaa gcggagagga aaatatatga atttatatag gcgggtttat ctcttacaac
     7081 tttattttcg gcctttcaaa aaaataatta aaatcgacag acacgaatca tttcgaccac
     7141 aggtaaagat aacgtgacct ggctgtcaga cagccttttc cctcgtgtta actaattttt
     7201 aaactaatta atcatctcag cccttggatt agttcttttg ctttgatggc ttcatgactg
     7261 tgacctgctc gatccgcgtg ttacatgaca gctccgtttt tttagtggtt aacttaaacc
     7321 gagtcaatcc aggcaacgtt agtcgtcgtc gtggttggct tgttcaatta gatttcatac
     7381 aattcaacgt aatttaattc gttttctatt agaattgtat cataattaat tcagaccgtg
     7441 aaagaaagtg tctttcatga tgtgtttatg gatatttata caataagata caatgtttca
     7501 tcatattcac tattcacgat tagtatgtac attaaataat ggctactact acatccgaac
     7561 tcgtcaaaac gattctgaat caattataca tatgctgact cttgcataca taaaaaatag
     7621 ttgtttaaat tttgtctaac taatgtttgg tataagtata atgttgagtt gagataccaa
     7681 ttacatcgag tctagccatt ttgtcgtgcc atattcgtca aaactttctt acataatgat
     7741 aacctagatc tagatgagat atgtatcaat gtatttgaga tcataattaa gttcgttcta
     7801 aattttgtcc ctgcaggtcc tgctgagcct cgacatgttg tcgcaaaatt cgccctggac
     7861 ccgcccaacg atttgtcgtc actgtcaagg tttgacctgc acttcatttg gggcccacat
     7921 acaccaaaaa aatgctgcat aattctcggg gcagcaagtc ggttacccgg ccgccgtgct
     7981 ggaccgggtt gaatggtgcc cgtaactttc ggtagagcgg acggccaata ctcaacttca
     8041 aggaatctca cccatgcgcg ccggcgggga accggagttc ccttcagtga gcgttattag
     8101 ttcgccgctc ggtgtgtcgt agatactagc ccctggggca cttttgaaat ttgaataaga
     8161 tttatgtaat cagtctttta ggtttgaccg gttctgccgc tttttttaaa attggatttg
     8221 taataataaa acgcaattgt ttgttattgt ggcgctctat catagatgtc gctataaacc
     8281 tattcagcac aatatattgt tttcatttta atattgtaca tataagtagt agggtacaat
     8341 cagtaaattg aacggagaat attattcata aaaatacgat agtaacgggt gatatattca
     8401 ttagaatgaa ccgaaaccgg cggtaaggat ctgagctaca catgctcagg ttttttacaa
     8461 cgtgcacaac agaattgaaa gcaaatatca tgcgatcata ggcgtctcgc atatctcatt
     8521 aaagcaggac tctagggact agtcccgggt cttaattaac tctctagagg cgcgccaagc
     8581 ttctactact aagatccacc agtatcctca tgccattcaa tcttctgagc ttcgaagata
     8641 tcgttaagtc caccagccat agatccctcg agtccggtgt gagtcctttg atgctcggtg
     8701 agcttatcac tcctagagaa agattttcca cactcagggc atttataagg tttttctcca
     8761 gtgtgtgtcc tttgatgagc cctaaggttt gctctctgag agaatgattt tccacactca
     8821 gggcatttat aaggtttttc accagtatgg gtcctttggt gcctaacaag gtttccagat
     8881 gttgagaatg attttccaca ctcagggcat ttataaggtt tttctccggt atgagtcctc
     8941 tgatgcctaa caagagatga agattgtgag aatgattttc cacactcagg gcatttataa
     9001 ggtttttcac cagtatgggt cctctgatgg gtggtaagat gatcactcct actgaatgat
     9061 tttccacact cagggcattt ataaggtttt tcaccggtat gggtcctttg gtgcctccta
     9121 agatctccag attgagagaa agattttcca cactcagggc atttataagg ctcgagaggt
     9181 ccttggaaaa gaacctcaag ggatcctttg tcgtcatcgt ctttgtagtc tttgtcgtca
     9241 tcgtctttgt agtctttgtc gtcatcgtct ttgtagtcgg taccaagctt ggcgcgcccg
     9301 tcccgggtct taattaactc tctagactca cctagcacca ctttgtacaa gaaagctggg
     9361 tcggcgcgcc cacccttaga agcttttgat ggttcttccc ctggagcgtc atcctcatca
     9421 tgatagatgt tttcagccat ttgccatatt tggagtatgt tgtcctcagc aacactggaa
     9481 ataacccaat cttcacaagg gttccacgag aaatctgaaa ttttgctagt gtgaccaccg
     9541 tgaataaaca gcagctcagg tgggccgtct tctgcgtcct ccactgtctg ttcctcatcg
     9601 atcctatcat aagacaccct caaacatatc aatattgctc ctccatgacc atacatgaac
     9661 attaaactaa agaactaaag tctacggccc aaacctgcta aggtcccaaa ccataagtct
     9721 tctaccaagg caacatgaag ctaaaatagt ctcgttcttt gggttccaac caacttgaaa
     9781 aacctcctct ctggagatca agcacataat taaggttagt gatgttgctg taacttgtaa
     9841 atacttctta gtaacacaaa atccatctca aagcggcaat atttatggcg tatcattctt
     9901 acttgtggct atcaaatgtg tgaagagcgg tgcttagctt ccgtagatcg aataatttaa
     9961 cagtcttgtc agttgatcct gttgccacaa cccactcatt aaatggattg aaggctaagc
    10021 agttaaccta aggcatttca aacaactcaa caattagcaa atacatacga ggcaatatga
    10081 ttcaatggtt tttagtgtta aggaaagcaa gcttaaaaac cagaagtatt atctgtatct
    10141 aaagctaata tattgcaaac tcacctccat cgaatgagca accacagact gtactggttt
    10201 actggcagat ggagaacgca gatcccatat gagaaggtat tggtcatctc caactgatcc
    10261 aaacaggtac tcatgtctca gatgccatgc aacatcttcc acaacccctt catgagcctt
    10321 aagttcagaa aataatcatc agcaaggaaa acacagaaac caacctgaat atctatctat
    10381 gagctttcat aagtgtaact gattccgtta agtgggtgca attccttcct ttctagatga
    10441 tacaaaacaa aactaaaaac gaatagaaat ccaaagcatt accttaaaaa tctgctgagc
    10501 gtccagagat ttgtttttag gagtagcgtt aatatcccac aagcagatct gagcatcatc
    10561 agagccacta agcaaatgac cctgcttgaa cttactccaa gagagaccat atccctcaga
    10621 gctgtgacct ctaagcttta aatcaggatt acaagcccca tcaagaggag gcttagatgg
    10681 atgcttactg taatcgaaaa catagacctc cgcattaact gtcttggtag caattatgaa
    10741 agggttttga ggcatatagc gagctcgatt aacctcacca tcatgattta tctgctggat
    10801 aatttgtacc tacacaagca atcaaactaa catttatcat cctttacctc gttataaccc
    10861 tagaatcaaa tctagaaagt tggaaatgag aaacaaaaat aaaaaatcgc acctttccag
    10921 ttgcacagcc aaagccacca aattcagagc ggtcatcatc gtactgacga gcttcactct
    10981 cggtatcgtc aagaggaagc tgaacttggg ctagcatcaa gtaattgggc tcgctctcgg
    11041 aggtatgggt accgagaatc atcttctgaa cggagtagtc tttaccggaa ggttcttcac
    11101 ggtcaggaag ccattcaaca gtaagtgaag gccactcgag agcgtgagta ataacgagat
    11161 cgtagaggaa aggagtgttc ttcttccaga tcttgtactc ttcattgatt agtcgctcct
    11221 cgatctcacc tcgcatttcc tcttcgtctt tccccatatc ggcgatgtct ttgttattcc
    11281 cgccacaaga acaaggcaaa accctaaaac agatctcttt acaaattgta agaaacttct
    11341 cgcttctgtt tctgaataat tcacatatag aagatgatga ataaaaaaag gaagaagata
    11401 aagaaaaggc ccatttttga aaagcccgtt tatgaaaagg cccaattaga tgacttttga
    11461 tcttacacca gtcaacagaa gtgaggattg gagaaaagag agaaaaaatg gcgatggaga
    11521 tcggagaaga tgaatggaag gtttgctgtg gaagctccga attcgcgaag cagatgtcta
    11581 cgtctggacc gttaacttct caagaagcaa tatatactgc aagagacatc tggttcaacc
    11641 aagttaatgt gacagattgg cttgaagctt tctctgctca tcctcagatt gggaatactc
    11701 cttcaccttc tatcaactcc gattttgctc gtcggagcgt atcagaacag tcaaccgcct
    11761 ttgccacaac ttctgcttct gctttacagg tgatggatct tcactttttt tttccttcat
    11821 ctcttcagtg atttcttctt ctgatgatca attgttttca atttcttagg agcttgccga
    11881 atggaatgta ctttacaaaa agaagtttgg ctttatcttc attatttgcg cttctggcag
    11941 gactcacgct gagatgctcc atgcgttaaa ggtcaacttt tgcatttcca aatcagtttt
    12001 tgttgagtgt gagtgaatga gaaagttaca tattcatcaa atcgtctctt tttcaggaaa
    12061 ggtatgaaaa taggccaata gtggagcttg agattgctgc tatggaacaa atgaaaataa
    12121 cagagctacg aatggcaaag ctgttctccg ataaggctaa agttatatca gaaactgaca
    12181 gctctagctc tcctgtttca acaaaacctc aaggtatatt gatgatttac caaatctttt
    12241 ccttgtcaaa gttttgtgtt tgactgtgtg ggtttgaacc tgttaggatt cagtatgata
    12301 tcaagtatgt gtcttttgga atacaaggat ttacccttat ggctatcttt gttatctgtg
    12361 tgaccttttc tactttctcg ctttgtaaga tcgtctgaga atcattggag ggcatttgaa
    12421 tgttgcagct gaagcaaaag ctcccaagag aagtaggcca ccgatcacga ctcatgtctt
    12481 agacgtctca cgtggtgctc cagctgcagg tgttgaagtg cacttagaag tgtggagtgg
    12541 tactaccggt ccttcctttg ttcatggagg tggtggggtc tggtccagtg tgggcacatc
    12601 agctactgat agagatgggc gcagtgggcc actgatggat ttggttgacg ctttgaatcc
    12661 agggacatat aggattagct tcgacactgc aaagtattcc ccaggctgct tctttcccta
    12721 tgtttccatt gtatttcagg ttacagaatc acagggtgaa gggggcggcc gcggagcctg
    12781 cttttttgta caaacttgtt gatgatcccg ggcccgcggt accgtcgact gcagaattca
    12841 attctgagat ttttcaaatc agtgcgcaag acgtgacgta agtatccgag tcagttttta
    12901 tttttctact aatttggtcg tttatttcgg cgtgtaggac atggcaaccg ggcctgaatt
    12961 tcgcgggtat tctgtttcta ttccaacttt ttcttgatcc gcagccatta acgacttttg
    13021 aatagatacg ctgacacgcc aagcctcgct agtcaaaagt gtaccaaaca acgctttaca
    13081 gcaagaacgg aatgcgcgtg acgctcgcgg tgacgccatt tcgccttttc agaaatggat
    13141 aaatagcctt gcttcctatt atatcttccc aaattaccaa tacattacac tagcatctga
    13201 atttcataac caatctcgat acaccaaatc gaatcgatga gcccagaacg acgcccggcc
    13261 gacatccgcc gtgccaccga ggcggacatg ccggcggtct gcaccatcgt caaccactac
    13321 atcgagacaa gcacggtcaa cttccgtacc gagccgcagg aaccgcagga gtggacggac
    13381 gacctcgtcc gtctgcggga gcgctatccc tggctcgtcg ccgaggtgga cggcgaggtc
    13441 gccggcatcg cctacgcggg cccctggaag gcacgcaacg cctacgactg gacggccgag
    13501 tcgaccgtgt acgtctcccc ccgccaccag cggacgggac tgggctccac gctctacacc
    13561 cacctgctga agtccctgga ggcacagggc ttcaagagcg tggtcgctgt catcgggctg
    13621 cccaacgacc cgagcgtgcg catgcacgag gcgctcggat atgccccccg cggcatgctg
    13681 cgggcggccg gcttcaagca cgggaactgg catgacgtgg gtttctggca gctggacttc
    13741 agcctgccgg taccgccccg tccggtcctg cccgtcaccg agatttgact gcagaatctt
    13801 ggactcccat gttggcaaag gcaaccaaac aaacaatgaa tgatccgctc ctgcatatgg
    13861 ggcggtttga gtatttcaac tgccatttgg gctgaattga agacatgctc ctgtcagaaa
    13921 ttccgtgatc ttactcaata ttcagtaatc tcggccaata tcctaaatgt gcgtggcttt
    13981 atctgtcttt gtattgtttc atcaattcat gtaacgtttg cttttcttat gaattttcaa
    14041 ataaattatc agatccgagc tcgaattaat tcggcgttaa ttcagtacat taaaaacgtc
    14101 cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca
    14161 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
    14221 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
    14281 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
    14341 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcacc
    14401 gcggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg
    14461 aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg
    14521 tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata
    14581 ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg
    14641 cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga
    14701 aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg
    14761 ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt
    14821 tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc
    14881 ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat
    14941 caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg
    15001 cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg
    15061 ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa
    15121 gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa
    15181 agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta
    15241 tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga
    15301 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt
    15361 actggatgaa ttgttttagt acctagaatg catgaccaaa atcccttaac gctgagagat
    15421 cccctcataa tttccccaaa gcgtaaccat gtgtgaataa attttgagct agtagggttg
    15481 cagccacgag taagtcttcc cttgttattg tgtagccaga atgccgcaaa acttccatgc
    15541 ctaagcgaac tgttgagagt acgtttcgat ttctgactgt gttagcctgg aagtgcttgt
    15601 cccaaccttg tttctgagca tgaacgcccg caagccaaca tgttagttga agcatcaggg
    15661 cgattagcag catgatatca aaacgctctg agctgctcgt tcggctatgg cgtaggccta
    15721 gtccgtaggc aggacttttc aagtctcgga aggtttcttc aatctgcatt cgcttcgaat
    15781 agatattaac aagttgtttg ggtgttcgaa tttcaacagg taagttagtt gctagaatcc
    15841 atggctcctt tgccgacgct gagtagattt taggtgacgg gtggtgacaa tgagtccgtg
    15901 tcgagcgctg attttttcgg cctttagagc gagatttata caatagaatt tggcatgaga
    15961 ttggattgct tttagtcagc ctcttatagc ctaaagtctt tgagtgacta gatgacatat
    16021 catgtaagtt gctgataggt ttccagtttt ccgctcctag gtctgcatat tgtacttttc
    16081 ctcttactcg acttaaccag taccaaccca gcttctcaac ggatttatac catggcactt
    16141 taaagccagc atcactgaca atgagcggtg tggtgttact cggtagaatg ctcgcaaggt
    16201 cggctagaaa ttggtcatga gctttctttg aacattgctc tgaaagcggg aacgctttct
    16261 cataaagagt aacagaacga ccgtgtagtg cgactgaagc tcgcaatacc ataagccgtt
    16321 tttgctcacg gatatcagac cagtcaacaa gtacaatggg catcgtattg cccgaacaga
    16381 taaagctagc atgccaacgg tatacagcga gtcgctcttt gtggaggtga cgattaccta
    16441 acaatcggtc gattcgtttg atgttatgtt ttgttctcgc tttggttggc aggttacggc
    16501 caagttcggt aagagtgaga gttttacagt caagtaaggc gtggcaagcc aacgttaagc
    16561 tgttgagtcg ttttaagtgt aattcggggc agaattggta aagagagtcg tgtaaaatat
    16621 cgagttcgca cattttgttg tctgattatt gatttttggc gaaaccattt gatcatatga
    16681 caagatgtgt atctacctta acttaatgat tttgataaaa atcattaggg gattcatcag
    16741 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    16801 ttc
//
