LOCUS       sequence-403959-        3468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In vitro transcription of RNA probes for in situ hybridization of 
            CeRep55 lncRNAs in nematode (after linearization with NotI and 
            BglI).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Tabara H, Mitani S, Mochizuki M, Kohara Y, Nagata K
  TITLE     A small RNA system ensures accurate homologous pairing and unpaired 
            silencing of meiotic chromosomes.
  JOURNAL   EMBO J. 2023 Jun 1;42(11):e105002. doi: 10.15252/embj.2020105002. 
            Epub 2023 Apr 20.
  PUBMED    37078421
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2023-06-1"; volume: "42(11):e105002. doi"; pages: " 
            10.15252/embj.2020105002. Epub 2023 Apr 20"
COMMENT     Sequence Label: pHIT353
FEATURES             Location/Qualifiers
     source          1..3468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        22..40
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1056..1074)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1114..1133)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1324..1345
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1508..1612
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1613..1681,1682..2473)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1831..1850)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2644..3232
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3133..3152
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3386..3403
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gttaacctgg cttatcgaaa ttaatacgac tcactatagg gagcgatcgc atgcagccaa
       61 ctcgagtaaa atatagtggg aagccacaga aagtaaaata tagtgggaag ccacagagag
      121 taaaatatag tgggaagcca cagagagtaa aataatcgat gaagccacag agagtaaaat
      181 atagtgggaa gccacagaga gtaaaatata gtgggaaacc acagagagta aaatatagtg
      241 ggaagccaca gagagtaaaa tatagtggga agccacagag agtaaaatat agtgggaagc
      301 cacagagagt aaaataatcg atgaagccac agagagtaaa atatagtggg aagccacaga
      361 gagtaaaata tagtgggaaa ccacagagag taaaatatag tgggaagcca cagagagtaa
      421 aatatagtgg gaagccacag agagtaaaat atagtgggaa gccacagaga gtaaaatata
      481 gtgggaagcc acagagagta aaatatagtg ggaagccaca gagagtaaag tatagtggga
      541 agccacagag agtaaaataa tcgatgaagc cacagagagt aaaatatagt gggaagccac
      601 agagagtaaa gtatagtggg aagccacaga gagtaaaata tagtgggaag ccacagagag
      661 taaaatatag tgggaagcca cagagagtaa aatatagtgg gaagccacag agagtaaaat
      721 aatcgatgaa gccacagaga gtaaaatata gtgggaagcc acagagagta aaatatagtg
      781 ggaagccaca gagagtaaaa tatagtggga agccacagag agtaaaataa tcgatgaagc
      841 cacagagttt tgaatttttt cttggcagtg tgctaacttt tccgacggat gtgacacaaa
      901 aaaaaggaaa attggggatt ttagttgaaa ttaggccata gtttcaagtt tttgacccgc
      961 cataaaacaa atttgactaa tttttaagaa gtgtctcgtt atgaaattcg gtcttttgaa
     1021 gctagttact gatggcgcga gtgcggccgc ttcgccctat agtgagtcgt attagcagcg
     1081 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     1141 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     1201 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     1261 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     1321 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     1381 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     1441 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     1501 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     1561 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     1621 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     1681 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     1741 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     1801 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     1861 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     1921 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     1981 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     2041 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     2101 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     2161 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     2221 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     2281 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     2341 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     2401 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     2461 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     2521 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     2581 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     2641 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     2701 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     2761 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     2821 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     2881 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     2941 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3001 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3061 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3121 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3181 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     3241 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     3301 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     3361 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     3421 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcag
//
