LOCUS       sequence-403960-       13976 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express TRB3-ZF108 in Arabidopsis target FWA gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13976)
  AUTHORS   Wang M, Zhong Z, Gallego-Bartolome J, Feng S, Shih YH, Liu M, Zhou 
            J, Richey JC, Ng C, Jami-Alahmadi Y, Wohlschlegel J, Wu K, Jacobsen 
            SE
  TITLE     Arabidopsis TRB proteins function in H3K4me3 demethylation by 
            recruiting JMJ14.
  JOURNAL   Nat Commun. 2023 Mar 28;14(1):1736. doi: 10.1038/s41467-023-37263-9.
  PUBMED    36977663
REFERENCE   2  (bases 1 to 13976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-03-28"; volume: "14(1):1736. doi"; pages: " 
            10.1038/s41467-023-37263-9"
COMMENT     Sequence Label: pMDC123-TRB3-ZF
FEATURES             Location/Qualifiers
     source          1..13976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    83..107
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     295..317
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     309..326
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     310..326
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2424..2468
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             2496..2519
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             3030..3101
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     protein_bind    3141..3165
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3187..3198
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(4090..4114)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4926..4942)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4926..4942)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4939..4961)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4950..4966
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4974..4980,4981..4998,4999..5004))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5019..5040
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5231..5907
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     5844..5864
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             5952..6503
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     polyA_signal    6510..6684
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    6762..6786
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7211..8005
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      8092..8680
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8581..8600
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8834..8851
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8866..9006
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8932..8951)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9092..9114
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(9168..9959)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      10367..10561
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10627..11700)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12129..12758)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 cacatacaaa tggacgaacg gataaacctt ttcacgccct tttaaatatc cgattattct
       61 aataaacgct cttttctctt aggtttaccc gccaatatat cctgtcaaac actgatagtt
      121 taaactgaag gcgggaaacg acaatctgat ccaagctcaa gctgctctag cattcgccat
      181 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
      241 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
      301 cacgacgttg taaaacgacg gccagtgcca agcttgcatg cctgcaggtc gactctagag
      361 gatccccggg taccgggccc cccctcgagg cgcgccacgc gtagtctagc tcaacagagc
      421 ttttaaccca aattggtaca atagaataca actttagatc ataattctca aaagaaagag
      481 attccttagc tattctatct gccactccat ttccttctcg gcttgtatgc acaagcataa
      541 aatcctcaaa cttgctaagt agatacttta tgtcttggat aattggattg agacttgaca
      601 agcataactt tcatgtaacc aaagacacaa gttgctgaga atccacctca aaaatgatct
      661 tcctataatt gaatcgggat aatgacagca cagcccatct aagagcctcc acttctactt
      721 ccagcacgct tcttactttt accacagctc ttgcacctaa ccataacacc ttccctgtat
      781 gatcgcgaag cacccaccct aagccacatt ttaatccttc tgttggccat gccccatcaa
      841 agttgcactt aacccaagat tgtggtggag cttcccatgt ttctcgtctg tcccgacggt
      901 gttgtggttg gtgctttcct tacattctga gcctctttcc ttctaatcca ctcatctgca
      961 tcttcttgtg tccttactaa tacctcattg gttccaaatt ccctcccttt aagcaccagc
     1021 tcgtttctgt tcttccacag cctcccaagt atccaaggga ctaaagcctc cacattcttc
     1081 agatcaggat attcttgttt aagatgttga actctatgga ggtttgtatg aactgatgat
     1141 ctaggaccgg ataagttccc ttcttcatag cgaacttatt caaagaatgt tttgtgtatc
     1201 attcttgtta cattgttatt aatgaaaaaa tattattggt cattggactg aacacgagtg
     1261 ttaaatatgg accaggcccc aaataagatc cattgatata tgaattaaat aacaagaata
     1321 aatcgagtca ccaaaccact tgcctttttt aacgagactt gttcaccaac ttgatacaaa
     1381 agtcattatc ctatgcaaat caataatcat acaaaaatat ccaataacac taaaaaatta
     1441 aaagaaatgg ataatttcac aatatgttat acgataaaga agttactttt ccaagaaatt
     1501 cactgatttt ataagcccac ttgcattaga taaatggcaa aaaaaaacaa aaaggaaaag
     1561 aaataaagca cgaagaattc tagaaaatac gaaatacgct tcaatgcagt gggacccacg
     1621 gttcaattat tgccaatttt cagctccacc gtatatttaa aaaataaaac gataatgcta
     1681 aaaaaatata aatcgtaacg atcgttaaat ctcaacggct ggatcttatg acgaccgtta
     1741 gaaattgtgg ttgtcgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca
     1801 cacgagtcgt gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca
     1861 attagccaaa aacaactttg cgtgtaaaca acgctcaata cacgtgtcat tttattatta
     1921 gctattgctt caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct
     1981 tcttctataa aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc
     2041 aaaatcttaa aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc
     2101 cttattctct caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt
     2161 tggtttagat tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat
     2221 catcttaatt ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt
     2281 tttgtcgaat aattactctt cgatttgtga tttctatcta gatctggtgt tagtttctag
     2341 tttgtgcgat cgaatttgtc gattaatctg agtttttctg attaacagac gcgtccatgg
     2401 ttgggtacca acaaatggct ggtggactta acgatatctt cgaagctcag aagattgaat
     2461 ggcatgagga tactggtgga tctagcatcc ccggacttga ggttcttttc caaggacctc
     2521 tcgagcctta taaatgccct gagtgtggaa aatctttctc tcaatctgga gatcttagga
     2581 ggcaccaaag gacccatacc ggtgaaaaac cttataaatg ccctgagtgt ggaaaatcat
     2641 tcagtaggag tgatcatctt accacccatc agaggaccca tactggtgaa aaaccttata
     2701 aatgccctga gtgtggaaaa tcattctcac aatcttcatc tcttgttagg catcagagga
     2761 ctcataccgg agaaaaacct tataaatgcc ctgagtgtgg aaaatcattc tcaacatctg
     2821 gaaaccttgt taggcaccaa aggacccata ctggtgaaaa accttataaa tgccctgagt
     2881 gtggaaaatc attctctcag agagcaaacc ttagggctca tcaaaggaca cacactggag
     2941 aaaaacctta taaatgccct gagtgtggaa aatctttctc taggagtgat aagctcaccg
     3001 agcatcaaag gactcacacc ggactcgagg actacaaaga cgatgacgac aaagactaca
     3061 aagacgatga cgacaaagac tacaaagacg atgacgacaa aaagcttgat atcgccatgg
     3121 ggggcgcgcc aagctatcaa acaagtttgt acaaaaaagc aggctccgcg gccgccccct
     3181 tcaccatggg agctccaaag ctgaagtgga caccagaaga agaaacagct cttaaagctg
     3241 gagtacttaa acatggaact ggaaaatggc gtaccattct ctcagatcct gtgtatagca
     3301 caattctgaa gtctcgttct aatgtggatc tcaaggacaa atggagaaat ataagtgtaa
     3361 cagctttatg gggatctagg aagaaggcta agcttgctct taaaagaact cctttgtcgg
     3421 gtagtaggca agacgataac gctacagcta ttaccattgt gtctttagct aatggtgatg
     3481 taggaggaca acagattgat gcaccatctc ctcctgcagg ttcttgtgaa cctccaagac
     3541 cttctacaag tgtggataag attatattgg aggctattac cagcttgaag agaccatttg
     3601 gtcctgacgg aaagtcgatc ttgatgtata tagaggagaa tttcaagatg caaccggata
     3661 tgaaacggct tgtgacttca aggctgaagt atttgacaaa tgttggaaca ttagttaaga
     3721 aaaagcacaa atacaggatt tctcagaatt atatggctga aggagaagga caaaggtctc
     3781 ctcaactctt gttggaagga aacaaggaaa acactccgaa accggaggag aatggcgtca
     3841 aaaatcttac taaatcccaa gtaggtggag aagttatgat aatgggtatg acagaaaaag
     3901 aagctgctgc agcagctgca agagctgtgg cagaagcaga atttgcaatg gcagaagctg
     3961 aagaagcagc aagagaggca gataaagcag aagccgaagc tgaagctgcc catatttttg
     4021 caaaagcggc gatgaaagct gtgaagtata gaatgcatag ccaaactcgg taaaagggtg
     4081 ggcgcgccga cccagctttc ttgtacaaag tggttcgata attcttaatt aactagttct
     4141 agagcggccg ccaccgcggt ggagctccta ggtgagtcta gagagttaat taagacccgg
     4201 gactagtccc tagagtcctg ctttaatgag atatgcgaga cgcctatgat cgcatgatat
     4261 ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag catgtgtagc tcagatcctt
     4321 accgccggtt tcggttcatt ctaatgaata tatcacccgt tactatcgta tttttatgaa
     4381 taatattctc cgttcaattt actgattgta ccctactact tatatgtaca atattaaaat
     4441 gaaaacaata tattgtgctg aataggttta tagcgacatc tatgatagag cgccacaata
     4501 acaaacaatt gcgttttatt attacaaatc caattttaaa aaaagcggca gaaccggtca
     4561 aacctaaaag actgattaca taaatcttat tcaaatttca aaagtgcccc aggggctagt
     4621 atctacgaca caccgagcgg cgaactaata acgctcactg aagggaactc cggttccccg
     4681 ccggcgcgca tgggtgagat tccttgaagt tgagtattgg ccgtccgctc taccgaaagt
     4741 tacgggcacc attcaacccg gtccagcacg gcggccgggt aaccgacttg ctgccccgag
     4801 aattatgcag catttttttg gtgtatgtgg gccccaaatg aagtgcaggt caaaccttga
     4861 cagtgacgac aaatcgttgg gcgggtccag ggcgaatttt gcgacgagct cgaattcgta
     4921 atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     4981 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     5041 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     5101 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg gctagagcag cttgccaaca
     5161 tggtggagca cgacactctc gtctactcca agaatatcaa agatacagtc tcagaagacc
     5221 aaagggctat tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt
     5281 gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat
     5341 gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca
     5401 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt
     5461 caaagcaagt ggattgatgt gaacatggtg gagcacgaca ctctcgtcta ctccaagaat
     5521 atcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaata
     5581 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcaa aaggacagta
     5641 gaaaaggaag gtggcaccta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
     5701 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
     5761 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac
     5821 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt
     5881 tcatttcatt tggagaggac acgctgaaat caccagtctc tctctacaaa tctatctctc
     5941 tcgagtctac catgagccca gaacgacgcc cggccgacat ccgccgtgcc accgaggcgg
     6001 acatgccggc ggtctgcacc atcgtcaacc actacatcga gacaagcacg gtcaacttcc
     6061 gtaccgagcc gcaggaaccg caggagtgga cggacgacct cgtccgtctg cgggagcgct
     6121 atccctggct cgtcgccgag gtggacggcg aggtcgccgg catcgcctac gcgggcccct
     6181 ggaaggcacg caacgcctac gactggacgg ccgagtcgac cgtgtacgtc tccccccgcc
     6241 accagcggac gggactgggc tccacgctct acacccacct gctgaagtcc ctggaggcac
     6301 agggcttcaa gagcgtggtc gctgtcatcg ggctgcccaa cgacccgagc gtgcgcatgc
     6361 acgaggcgct cggatatgcc ccccgcggca tgctgcgggc ggccggcttc aagcacggga
     6421 actggcatga cgtgggtttc tggcagctgg acttcagcct gccggtaccg ccccgtccgg
     6481 tcctgcccgt caccgagatt tgactcgagt ttctccataa taatgtgtga gtagttccca
     6541 gataagggaa ttagggttcc tatagggttt cgctcatgtg ttgagcatat aagaaaccct
     6601 tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca
     6661 aaatccagta ctaaaatcca gatcccccga attaattcgg cgttaattca gtacattaaa
     6721 aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc
     6781 ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat
     6841 acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag gcggcagact
     6901 ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac
     6961 ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg
     7021 atcaccgcgg tttcaaaatc ggctccgtcg atactatgtt atacgccaac tttgaaaaca
     7081 actttgaaaa agctgttttc tggtatttaa ggttttagaa tgcaaggaac agtgaattgg
     7141 agttcgtctt gttataatta gcttcttggg gtatctttaa atactgtaga aaagaggaag
     7201 gaaataataa atggctaaaa tgagaatatc accggaattg aaaaaactga tcgaaaaata
     7261 ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag gtatataagc tggtgggaga
     7321 aaatgaaaac ctatatttaa aaatgacgga cagccggtat aaagggacca cctatgatgt
     7381 ggaacgggaa aaggacatga tgctatggct ggaaggaaag ctgcctgttc caaaggtcct
     7441 gcactttgaa cggcatgatg gctggagcaa tctgctcatg agtgaggccg atggcgtcct
     7501 ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag attatcgagc tgtatgcgga
     7561 gtgcatcagg ctctttcact ccatcgacat atcggattgt ccctatacga atagcttaga
     7621 cagccgctta gccgaattgg attacttact gaataacgat ctggccgatg tggattgcga
     7681 aaactgggaa gaagacactc catttaaaga tccgcgcgag ctgtatgatt ttttaaagac
     7741 ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac ctgggagaca gcaacatctt
     7801 tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg agaagcggca gggcggacaa
     7861 gtggtatgac attgccttct gcgtccggtc gatcagggag gatatcgggg aagaacagta
     7921 tgtcgagcta ttttttgact tactggggat caagcctgat tgggagaaaa taaaatatta
     7981 tattttactg gatgaattgt tttagtacct agaatgcatg accaaaatcc cttaacgtga
     8041 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     8101 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     8161 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     8221 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     8281 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     8341 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     8401 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     8461 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     8521 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     8581 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     8641 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     8701 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     8761 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     8821 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt
     8881 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
     8941 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg
     9001 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     9061 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     9121 ccgtcatcac cgaaacgcgc gaggcagggt gccttgatgt gggcgcctta tttgccgact
     9181 accttggtga tctcgccttt cacgtagtgg acaaattctt ccaactgatc tgcgcgcgag
     9241 gccaagcgat cttcttcttg tccaagataa gcctgtctag cttcaagtat gacgggctga
     9301 tactgggccg gcaggcgctc cattgcccag tcggcagcga catccttcgg cgcgattttg
     9361 ccggttactg cgctgtacca aatgcgggac aacgtaagca ctacatttcg ctcatcgcca
     9421 gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc
     9481 tgttcaggaa ccggatcaaa gagttcctcc gccgctggac ctaccaaggc aacgctatgt
     9541 tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga tcgtggctgg ctcgaagata
     9601 cctgcaagaa tgtcattgcg ctgccattct ccaaattgca gttcgcgctt agctggataa
     9661 cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt ctacagcgcg gagaatctcg
     9721 ctctctccag gggaagccga agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt
     9781 tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat cactgtgtgg cttcaggccg
     9841 ccatccactg cggagccgta caaatgtacg gccagcaacg tcggttcgag atggcgctcg
     9901 atgacgccaa ctacctctga tagttgagtc gatacttcgg cgatcaccgc ttccctcata
     9961 atgtttaact ttgttttagg gcgactgccc tgctgcgtaa catcgttgct gctccataac
    10021 atcaaacatc gacccacggc gtaacgcgct tgctgcttgg atgcccgagg catagactgt
    10081 accccaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg
    10141 cgttcggtca aggttctgga ccagttgcgt gagcgcatgg cgccggcggt cgagtggcga
    10201 cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt aggccagcca tttttgagcg
    10261 gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt agggagcgca gcgaccgaag
    10321 ggtaggcgct ttttgcagct cttcggctgt gcgctggcca gacagttatg cacaggccag
    10381 gcgggtttta agagttttaa taagttttaa agagttttag gcggaaaaat cgcctttttt
    10441 ctcttttata tcagtcactt acatgtgtga ccggttccca atgtacggct ttgggttccc
    10501 aatgtacggg ttccggttcc caatgtacgg ctttgggttc ccaatgtacg tgctatccac
    10561 aggaaagaga ccttttcgac ctttttcccc tgctagggca atttgcccta gcatctgctc
    10621 cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc gcatgactag
    10681 gatcgggcca gcctgccccg cctcctcctt caaatcgtac tccggcaggt catttgaccc
    10741 gatcagcttg cgcacggtga aacagaactt cttgaactct ccggcgctgc cactgcgttc
    10801 gtagatcgtc ttgaacaacc atctggcttc tgccttgcct gcggcgcggc gtgccaggcg
    10861 gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa ccgtcagcac
    10921 gtccgggttc ttgccttctg tgatctcgcg gtacatccaa tcagctagct cgatctcgat
    10981 gtactccggc cgcccggttt cgctctttac gatcttgtag cggctaatca aggcttcacc
    11041 ctcggatacc gtcaccaggc ggccgttctt ggccttcttc gtacgctgca tggcaacgtg
    11101 cgtggtgttt aaccgaatgc aggtttctac caggtcgtct ttctgctttc cgccatcggc
    11161 tcgccggcag aacttgagta cgtccgcaac gtgtggacgg aacacgcggc cgggcttgtc
    11221 tcccttccct tcccggtatc ggttcatgga ttcggttaga tgggaaaccg ccatcagtac
    11281 caggtcgtaa tcccacacac tggccatgcc ggccggccct gcggaaacct ctacgtgccc
    11341 gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg tcacgcttcg acagacggaa
    11401 aacggccacg tccatgatgc tgcgactatc gcgggtgccc acgtcataga gcatcggaac
    11461 gaaaaaatct ggttgctcgt cgcccttggg cggcttccta atcgacggcg caccggctgc
    11521 cggcggttgc cgggattctt tgcggattcg atcagcggcc gcttgccacg attcaccggg
    11581 gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc gcggccttca acttctccac
    11641 caggtcatca cccagcgccg cgccgatttg taccgggccg gatggtttgc gaccgctcac
    11701 gccgattcct cgggcttggg ggttccagtg ccattgcagg gccggcaggc aacccagccg
    11761 cttacgcctg gccaaccgcc cgttcctcca cacatggggc attccacggc gtcggtgcct
    11821 ggttgttctt gattttccat gccgcctcct ttagccgcta aaattcatct actcatttat
    11881 tcatttgctc atttactctg gtagctgcgc gatgtattca gatagcagct cggtaatggt
    11941 cttgccttgg cgtaccgcgt acatcttcag cttggtgtga tcctccgccg gcaactgaaa
    12001 gttgacccgc ttcatggctg gcgtgtctgc caggctggcc aacgttgcag ccttgctgct
    12061 gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt ttgctcattt tctctttacc
    12121 tcattaactc aaatgagttt tgatttaatt tcagcggcca gcgcctggac ctcgcgggca
    12181 gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg cggcggcagt gcctgggtag
    12241 ctcacgcgct gcgtgatacg ggactcaaga atgggcagct cgtacccggc cagcgcctcg
    12301 gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa ggccgcttgt
    12361 agccttccat ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc ggcggtggcc
    12421 catatgtcgt aagggcttgg ctgcaccgga atcagcacga agtcggctgc cttgatcgcg
    12481 gacacagcca agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc gcgccggccg
    12541 atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt tagcggttga
    12601 tcttcccgca cggccgccca atcgcgggca ctgccctggg gatcggaatc gactaacaga
    12661 acatcggccc cggcgagttg cagggcgcgg gctagatggg ttgcgatggt cgtcttgcct
    12721 gacccgcctt tctggttaag tacagcgata accttcatgc gttccccttg cgtatttgtt
    12781 tatttactca tcgcatcata tacgcagcga ccgcatgacg caagctgttt tactcaaata
    12841 cacatcacct ttttagacgg cggcgctcgg tttcttcagc ggccaagctg gccggccagg
    12901 ccgccagctt ggcatcagac aaaccggcca ggatttcatg cagccgcacg gttgagacgt
    12961 gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc cgcctcgatc tcttcggtaa
    13021 tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat gcttgttcct cttggcgttc
    13081 attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg tcctcacgga aggcaccgcg
    13141 ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt acagggtcga
    13201 gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg cggccttcct ggtcgatcag
    13261 ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg cgggggccaa acttcacgcc
    13321 tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa
    13381 ctcggcaatg ccggcgaaca cggtcaacac catgcggccg gccggcgtgg tggtgtcggc
    13441 ccacggctct gccaggctac gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc
    13501 cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg gtcactgtca caacgtcgcc
    13561 agggcgtagg tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat
    13621 cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa
    13681 gtcctggtcg tcggtgctga cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt
    13741 catggcgtaa tgtctccggt tctagtcgca agtattctac tttatgcgac taaaacacgc
    13801 gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt aggacttgtg
    13861 cgacatgtcg ttttcagaag acggctgcac tgaacgtcag aagccgactg cactatagca
    13921 gcggaggggt tggatcaaag tactttgatc ccgaggggaa ccctgtggtt ggcatg
//
