LOCUS       sequence-403961-       17599 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express TRB1-ZF108 in Arabidopsis target FWA gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17599)
  AUTHORS   Wang M, Zhong Z, Gallego-Bartolome J, Feng S, Shih YH, Liu M, Zhou 
            J, Richey JC, Ng C, Jami-Alahmadi Y, Wohlschlegel J, Wu K, Jacobsen 
            SE
  TITLE     Arabidopsis TRB proteins function in H3K4me3 demethylation by 
            recruiting JMJ14.
  JOURNAL   Nat Commun. 2023 Mar 28;14(1):1736. doi: 10.1038/s41467-023-37263-9.
  PUBMED    36977663
REFERENCE   2  (bases 1 to 17599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-03-28"; volume: "14(1):1736. doi"; pages: " 
            10.1038/s41467-023-37263-9"
COMMENT     Sequence Label: pEG302-TRB1-ZF
FEATURES             Location/Qualifiers
     source          1..17599
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..915
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(841..860)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1001..1023
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      1259..1453
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1519..2592)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3021..3650)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4951..4975
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     5163..5185
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5177..5194
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5178..5194
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    5217..5391
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(5394..6359)
                     /codon_start=1
                     /gene="birA"
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             complement(5397..6359)
                     /codon_start=1
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     terminator      complement(7820..8527)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     CDS             complement(8607..8651)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             complement(9177..9200)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(9207..9254)
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     protein_bind    9313..9337
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             13131..13139
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    complement(13571..13595)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        13647..14027
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             14033..14584
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      14594..14846
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    14932..14956
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             15381..16175
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
      841 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
      901 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
      961 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     1021 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg
     1081 gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc
     1141 catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg
     1201 cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta
     1261 tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa
     1321 atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg
     1381 ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta
     1441 cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc
     1501 tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta
     1561 gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag
     1621 gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct
     1681 gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg
     1741 gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg
     1801 aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag
     1861 ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat
     1921 caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg
     1981 catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt
     2041 tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg
     2101 gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac
     2161 cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac
     2221 ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt
     2281 cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata
     2341 gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg
     2401 cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca
     2461 cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt
     2521 caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt
     2581 gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
     2641 gcaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
     2701 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
     2761 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
     2821 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
     2881 cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc
     2941 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
     3001 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
     3061 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
     3121 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
     3181 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
     3241 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
     3301 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
     3361 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
     3421 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
     3481 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
     3541 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
     3601 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
     3661 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
     3721 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
     3781 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
     3841 cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
     3901 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
     3961 ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg
     4021 gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg
     4081 gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc
     4141 ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc
     4201 aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag
     4261 ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt
     4321 ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg
     4381 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
     4441 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
     4501 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
     4561 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
     4621 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
     4681 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
     4741 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
     4801 tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg
     4861 ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg
     4921 attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac
     4981 tgatagttta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
     5041 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     5101 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     5161 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcagggatc
     5221 tggattttag tactggattt tggttttagg aattagaaat tttattgata gaagtatttt
     5281 acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg aaaccctata
     5341 ggaaccctaa ttcccttatc tgggaactac tcacacatta ttatggagaa agcttatttt
     5401 tctgcactac gcagggatat ttcaccgccc atccagggtt ttattattcc atcctgctca
     5461 agtaataaag ccccctgttt gtctattccg cgtgaaatgc caaatatttc tttatcacca
     5521 atgataagtt tcactgggcg attaataaaa ttatccagct tttcccagcg cgacagataa
     5581 ggtgccaatc cttcttgttc gaagagttcc aacgcagcac gtaattcacg tattagcatg
     5641 gccgccaacg tattacgatc gagattgatc cccgcttcct gcagcgtgat ccacccctga
     5701 ttaacgacac tctcttcaac acggcgcatt gccatgttga tcccggctcc aatgactatt
     5761 tgcgccgcat cgccagtttt gccagtcagc tccaccagaa tgcctgccag cttgcgatcc
     5821 tgcagataga ggtcattagg ccatttaaca cgaactttat ctgcacccag cttgcgtaat
     5881 acttccgcca tcacgatacc gataaccaga cttaaaccaa tcgccgccgc cgggccttgt
     5941 tccagacgcc agaacatcga caaatataag tttgcgccaa aaggcgaaaa ccatttccga
     6001 ccccggcgac cacggccagc ctgctggtat tctgcaatgc aagcatcgcc cgatttaagc
     6061 tctccgatac gatcaagaag gtactgattc gtggagtcaa tcactggcag cacggctaca
     6121 ctaccgccat ccagctgacc caatatctgt ttagcattaa gtaactggat aggctcaggc
     6181 aggctgtatc ctttacccgg aacggtaaag acatcaacgc cccagtcacg cagtgtctga
     6241 atgtgtttat taatagccgc ccggctcatt cccagcgttt cacccaactg ctcgccagag
     6301 tgaaattcac cgttcgctaa cagggcaatc aatttcagtg gcacggtgtt atccttcatc
     6361 tcgaggtcac acacgattgg ttgaatatca tcagcctcag ccatttttta tgagctgcaa
     6421 acacacaaaa agagttcaat acagtcaaat aaaccctgat tgaatcagaa atagctttct
     6481 gttcaacgta cgacactaca aatcctaaag tttcgaagaa tctatacaca aacttcatct
     6541 aaccttaaac agtgttcaga ttcaatctaa cattgacagt gttcagattc aatctaactt
     6601 caacagttca gatttcaatt cacatataca aatcatttcc caggtaacat gaacatggat
     6661 ctctccatca aggtcaagcc aaattctggt agctataatc gagctaactg atcggtcaac
     6721 ttagatcgat tctagtaaaa ccaaaagatt tagtggaggt tcacagatcc aaattaaacg
     6781 atatccagat ctcaaggaaa ttgagaaaac aagatcgaga tccagcaaat aaagtagatc
     6841 cagaaagttc ctattacctt ggaaagaaag agcggaagaa gatgagattg aggaagattc
     6901 aaaacggaga atgaatctcc tggattatct cttactttct ctcttagtct tcttccttgt
     6961 tcttctctgt caagtcgccg gagattcaaa acggctgatg aaagtgagga ggacaacgag
     7021 acaattcaaa gcggagagga aaatatatga atttatatag gcgggtttat ctcttacaac
     7081 tttattttcg gcctttcaaa aaaataatta aaatcgacag acacgaatca tttcgaccac
     7141 aggtaaagat aacgtgacct ggctgtcaga cagccttttc cctcgtgtta actaattttt
     7201 aaactaatta atcatctcag cccttggatt agttcttttg ctttgatggc ttcatgactg
     7261 tgacctgctc gatccgcgtg ttacatgaca gctccgtttt tttagtggtt aacttaaacc
     7321 gagtcaatcc aggcaacgtt agtcgtcgtc gtggttggct tgttcaatta gatttcatac
     7381 aattcaacgt aatttaattc gttttctatt agaattgtat cataattaat tcagaccgtg
     7441 aaagaaagtg tctttcatga tgtgtttatg gatatttata caataagata caatgtttca
     7501 tcatattcac tattcacgat tagtatgtac attaaataat ggctactact acatccgaac
     7561 tcgtcaaaac gattctgaat caattataca tatgctgact cttgcataca taaaaaatag
     7621 ttgtttaaat tttgtctaac taatgtttgg tataagtata atgttgagtt gagataccaa
     7681 ttacatcgag tctagccatt ttgtcgtgcc atattcgtca aaactttctt acataatgat
     7741 aacctagatc tagatgagat atgtatcaat gtatttgaga tcataattaa gttcgttcta
     7801 aattttgtcc ctgcaggtcc tgctgagcct cgacatgttg tcgcaaaatt cgccctggac
     7861 ccgcccaacg atttgtcgtc actgtcaagg tttgacctgc acttcatttg gggcccacat
     7921 acaccaaaaa aatgctgcat aattctcggg gcagcaagtc ggttacccgg ccgccgtgct
     7981 ggaccgggtt gaatggtgcc cgtaactttc ggtagagcgg acggccaata ctcaacttca
     8041 aggaatctca cccatgcgcg ccggcgggga accggagttc ccttcagtga gcgttattag
     8101 ttcgccgctc ggtgtgtcgt agatactagc ccctggggca cttttgaaat ttgaataaga
     8161 tttatgtaat cagtctttta ggtttgaccg gttctgccgc tttttttaaa attggatttg
     8221 taataataaa acgcaattgt ttgttattgt ggcgctctat catagatgtc gctataaacc
     8281 tattcagcac aatatattgt tttcatttta atattgtaca tataagtagt agggtacaat
     8341 cagtaaattg aacggagaat attattcata aaaatacgat agtaacgggt gatatattca
     8401 ttagaatgaa ccgaaaccgg cggtaaggat ctgagctaca catgctcagg ttttttacaa
     8461 cgtgcacaac agaattgaaa gcaaatatca tgcgatcata ggcgtctcgc atatctcatt
     8521 aaagcaggac tctagggact agtcccgggt cttaattaac tctctagagg cgcgccaagc
     8581 ttctactact aagatccacc agtatcctca tgccattcaa tcttctgagc ttcgaagata
     8641 tcgttaagtc caccagccat agatccctcg agtccggtgt gagtcctttg atgctcggtg
     8701 agcttatcac tcctagagaa agattttcca cactcagggc atttataagg tttttctcca
     8761 gtgtgtgtcc tttgatgagc cctaaggttt gctctctgag agaatgattt tccacactca
     8821 gggcatttat aaggtttttc accagtatgg gtcctttggt gcctaacaag gtttccagat
     8881 gttgagaatg attttccaca ctcagggcat ttataaggtt tttctccggt atgagtcctc
     8941 tgatgcctaa caagagatga agattgtgag aatgattttc cacactcagg gcatttataa
     9001 ggtttttcac cagtatgggt cctctgatgg gtggtaagat gatcactcct actgaatgat
     9061 tttccacact cagggcattt ataaggtttt tcaccggtat gggtcctttg gtgcctccta
     9121 agatctccag attgagagaa agattttcca cactcagggc atttataagg ctcgagaggt
     9181 ccttggaaaa gaacctcaag ggatcctttg tcgtcatcgt ctttgtagtc tttgtcgtca
     9241 tcgtctttgt agtcggtacc aagcttggcg cgcccgtccc gggtcttaat taactctcta
     9301 gactcaccta gcaccacttt gtacaagaaa gctgggtcgg cgcgcccacc cttggcacgg
     9361 atcatctgtc gaatcacatt ttcaacaaaa gcattttgag attttcaaag ttctctcata
     9421 gatataatgt cttgtgatat aaaagaatat gggtgttttt ttcttttttt taccattttg
     9481 cagatgtttc ttcctttcaa tgtctttgaa gcttcctcag cgaaagcttg agctgcttct
     9541 gcttcggctt ctgctgcctc cgcttccttt gcagcttcct cagcctctgc catggcagct
     9601 tctgcctctg ctactgcctg tgctgcaact gctgccgcct catgtacgtt catactcttc
     9661 attctggcta tctctgtatc aatctgtgac cttgtctgaa aattcacttc atctatgtct
     9721 gttttaggtg aaggcaaaga agaagtcctc tgttttcctc caaacacccc cagacctttt
     9781 ctcctgtggg aagacaaggg agtggagttt ggaattctgt acttacgttt aacctgtaaa
     9841 agagaaagcc cagatcaata aacctcgcaa gttgcagtga ataatggata tgtctctgtt
     9901 ctactgacat ccatgaacta aattgatttg cacaaaaagt catacatact attcaaaaga
     9961 ccaaaaatat atgcactcaa aagcattaaa tgaatgtagg aattcttacc ttcacaagtt
    10021 tcccgcaaga tgtcaagtac tttagcttgg tagacaacaa gcgtttgaag tctggtggag
    10081 catgatattg atcctacaca aaaaaaaaaa aggaacacgg gtacagtcag tcctctaaat
    10141 cagtctttta caaggaactt agacaaagaa tcttcaaagc agtaatcgta caagttgaac
    10201 tcaatatttc ccatgcttga atcacataag tagacgaaaa tatttatgtc attggcgtgt
    10261 ttctgaatta actacagaat ctactcttac gatattctca taattatatc gacttaagta
    10321 gatgccacca caataaatct cgtaattaca gaaatatttc tagccctttc agcttagttt
    10381 cagtcaggat attcaatgca cattcgtcta ttcaggagag gagaagataa agatgtcacc
    10441 cattgccttg caataaaact gccacatccg ttgcctatat tgatattgag gaatatattc
    10501 tattgacaca accaaaggct atattttggt tttcatccgc aatgcttcac aggttctagt
    10561 tgacaacata tcaacccact gaggatcatt caaattgaaa tgtaacacga tgcgatttag
    10621 ctgagagtaa caatttaata atacatattg tctatgagaa aagaaaaaaa agatgcaaga
    10681 acgaaaacag aatacctcaa tgtaggcacc aattgttgtt ttgttacagc caccaggttc
    10741 tttcagggta gctattgctt ccattataag gctatccaac ctgcaacgtt tttaaaagtt
    10801 acaatttgtc tataataaaa gatttgagtg atacaaacat ctacgtgtaa gtgggacttt
    10861 acaaatcctc atcataattt cacaattata aagccacagt gataggattg agcaaacggc
    10921 tcgcatgata atagtaagaa ggaggagaaa tgtactaagc cagtgaagac aacttaaaca
    10981 gaggtgtgaa aacaaaccca tatcacatga atgtaatcca acacttatcg acttgcacag
    11041 aataaaaagt gacggcaaac catacatttt gagatgtaat ccaacttcag aaataccagc
    11101 gagagagata caacaaagca actcctcttc aatacaagaa acaaaataga cacatttcta
    11161 accttacatt aggtctcctt ggaggaggat tagaagaaac ttggagtcca gaggtcgcat
    11221 ccacattttc ttcatcactt tgcaaagaat tggttagggc tagtgagttc tcttcctgtt
    11281 taggaaggga gaacgtcctt ttaacagcta acctagactt ttcgcgcgat ccccagccat
    11341 ttgccatgac actcatgttt ctccatttgt cctgtcccaa tacaataaac aaaccatctc
    11401 ccagtcagat aaaatcacaa acaaaataca gtagcattat caaagctgac aaaaaggata
    11461 agaagtaatc atgaccttaa gatcaacatt ggaacgcagg tacaagactc cgctaaattc
    11521 aggatctttg agaattgtgc gccatttacc aggtccatgc tttataactc cagactttaa
    11581 agctgactct tcttcttgag tccatttctg cttaggagca cccatcccga tttacggtct
    11641 gctgtgtcaa agcaagtccg ttctgcaaca atacatatca aatgtcacga aaccaatcag
    11701 aactaaagtt tctacataaa aatgtaaact ttttcttcag ttgtagctca caattcatca
    11761 tcaaatacca cagaatagta aaaccctaat tccctgttcc gaggaaaccc taacatagat
    11821 tatcaaacta taccgcagca atagttcaga aactaagacg caatagcaaa caccaacttc
    11881 acatcactga aagatgccga aactcaaacc ctagaaaaat ccgttctttc agaatgaagc
    11941 ctaatctcat gctgagaata tacacaatcg agaaagaaga aacagatgaa ttcaccagga
    12001 atcagagttt agaggaagaa gaatcgatca ttcgcaatac caatttttgt ttaatctctg
    12061 gattcagatt tcgagacgct tgggaagcag aaaaagttag ggttaatgcg aatacaagga
    12121 ttcagagtaa cggacctccg tcaattctga tgcggcaaac cgcttagacc aattaagctt
    12181 taaacgcgtt ttttcacaaa cggccttaaa acgcgttttt tacaaacggc cttaaaacgc
    12241 gtttttgttt acgctagtta ttatattctg aagattataa attgtctaaa tatttacaaa
    12301 ttcaactttt aataatgata aagaaaatat caaattcccc aaatgaagtg ttaatatgat
    12361 tgtcaagaaa atatattatc aaaactaata cgcagtgacc cagatcatat ttacacgaac
    12421 aatttggttt atgtttgaaa gttttggtgg tgattaaaaa tttgatgatt aagtggtatt
    12481 ttgaacataa aatacaaata tgttgctaga tataattcag aaatgtgtgg tggggtggta
    12541 gttgtgatat tttttttcaa aaatgctatt ttcattttat ccatagaatt ttctctattg
    12601 tttatttttt ctgaaaatta tctagttgac tgtaccacta attaaaaaaa aattgtggac
    12661 atggtagttg cgatattttg agagttttcc aaatctatac acttgtttta aacacattaa
    12721 aaagttttat aatttattta tttttgaatt tctccaaaat ttgaaatata aagatcattt
    12781 cgtaaaaaaa agaaaagttc gttgaacata gttttagttg atttgtttta ctaggtttat
    12841 tttctgcaga tgttcagtaa aaagaaagaa ccttctctaa tttcacctag atatttatga
    12901 ttttataata caaactaatt tcatatttgg gtttaaatta ataattgtcc aaataatttc
    12961 aaattacagt caaaataaaa cattagcaat tagttccatc caaaataaat tattaaatta
    13021 ccaaattaac atttttagct catgagccca aaattattta tgatatgaaa tatgtacttg
    13081 ataataattc aagtcagcaa caataattca tcaaatttat ttcaaaataa acctccttaa
    13141 atctttctgg attttttaac tgataatcct attgttttat cttattttca ttaagccaac
    13201 aataagtcat accaattagt cattagttat aattcttgat attggttaaa gagaatgttt
    13261 aagtggtgga tggagttagt tgtatacttt tttggcatta agatgagaat aatttgctac
    13321 ttataaagac tttgtaatat taaacatcat attcttcaca tgaatataca agtggacaat
    13381 tctggttaag tcacaccagt ggtttaatcc ggttcatttt tctacgtata gcattagtgt
    13441 taattacgtg cacgagaaca caagacagaa agtaaaaaaa gcaagaaaga gtcaacaaag
    13501 ttgcccgcct aacgattact tagcactttt gaatccgttt taagcaaagc ggtgaagggg
    13561 gcggccgcgg agcctgcttt tttgtacaaa cttgttgatg atcccgggcc cgcggtaccg
    13621 tcgactgcag aattcaattc tgagattttt caaatcagtg cgcaagacgt gacgtaagta
    13681 tccgagtcag tttttatttt tctactaatt tggtcgttta tttcggcgtg taggacatgg
    13741 caaccgggcc tgaatttcgc gggtattctg tttctattcc aactttttct tgatccgcag
    13801 ccattaacga cttttgaata gatacgctga cacgccaagc ctcgctagtc aaaagtgtac
    13861 caaacaacgc tttacagcaa gaacggaatg cgcgtgacgc tcgcggtgac gccatttcgc
    13921 cttttcagaa atggataaat agccttgctt cctattatat cttcccaaat taccaataca
    13981 ttacactagc atctgaattt cataaccaat ctcgatacac caaatcgaat cgatgagccc
    14041 agaacgacgc ccggccgaca tccgccgtgc caccgaggcg gacatgccgg cggtctgcac
    14101 catcgtcaac cactacatcg agacaagcac ggtcaacttc cgtaccgagc cgcaggaacc
    14161 gcaggagtgg acggacgacc tcgtccgtct gcgggagcgc tatccctggc tcgtcgccga
    14221 ggtggacggc gaggtcgccg gcatcgccta cgcgggcccc tggaaggcac gcaacgccta
    14281 cgactggacg gccgagtcga ccgtgtacgt ctccccccgc caccagcgga cgggactggg
    14341 ctccacgctc tacacccacc tgctgaagtc cctggaggca cagggcttca agagcgtggt
    14401 cgctgtcatc gggctgccca acgacccgag cgtgcgcatg cacgaggcgc tcggatatgc
    14461 cccccgcggc atgctgcggg cggccggctt caagcacggg aactggcatg acgtgggttt
    14521 ctggcagctg gacttcagcc tgccggtacc gccccgtccg gtcctgcccg tcaccgagat
    14581 ttgactgcag aatcttggac tcccatgttg gcaaaggcaa ccaaacaaac aatgaatgat
    14641 ccgctcctgc atatggggcg gtttgagtat ttcaactgcc atttgggctg aattgaagac
    14701 atgctcctgt cagaaattcc gtgatcttac tcaatattca gtaatctcgg ccaatatcct
    14761 aaatgtgcgt ggctttatct gtctttgtat tgtttcatca attcatgtaa cgtttgcttt
    14821 tcttatgaat tttcaaataa attatcagat ccgagctcga attaattcgg cgttaattca
    14881 gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc
    14941 acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc
    15001 accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag
    15061 gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg
    15121 tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt
    15181 gctgcctgtg atcaccgcgg tttcaaaatc ggctccgtcg atactatgtt atacgccaac
    15241 tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa tgcaaggaac
    15301 agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa atactgtaga
    15361 aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg aaaaaactga
    15421 tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag gtatataagc
    15481 tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat aaagggacca
    15541 cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag ctgcctgttc
    15601 caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg agtgaggccg
    15661 atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag attatcgagc
    15721 tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt ccctatacga
    15781 atagcttaga cagccgctta gccgaattgg attacttact gaataacgat ctggccgatg
    15841 tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag ctgtatgatt
    15901 ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac ctgggagaca
    15961 gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg agaagcggca
    16021 gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag gatatcgggg
    16081 aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat tgggagaaaa
    16141 taaaatatta tattttactg gatgaattgt tttagtacct agaatgcatg accaaaatcc
    16201 cttaacgctg agagatcccc tcataatttc cccaaagcgt aaccatgtgt gaataaattt
    16261 tgagctagta gggttgcagc cacgagtaag tcttcccttg ttattgtgta gccagaatgc
    16321 cgcaaaactt ccatgcctaa gcgaactgtt gagagtacgt ttcgatttct gactgtgtta
    16381 gcctggaagt gcttgtccca accttgtttc tgagcatgaa cgcccgcaag ccaacatgtt
    16441 agttgaagca tcagggcgat tagcagcatg atatcaaaac gctctgagct gctcgttcgg
    16501 ctatggcgta ggcctagtcc gtaggcagga cttttcaagt ctcggaaggt ttcttcaatc
    16561 tgcattcgct tcgaatagat attaacaagt tgtttgggtg ttcgaatttc aacaggtaag
    16621 ttagttgcta gaatccatgg ctcctttgcc gacgctgagt agattttagg tgacgggtgg
    16681 tgacaatgag tccgtgtcga gcgctgattt tttcggcctt tagagcgaga tttatacaat
    16741 agaatttggc atgagattgg attgctttta gtcagcctct tatagcctaa agtctttgag
    16801 tgactagatg acatatcatg taagttgctg ataggtttcc agttttccgc tcctaggtct
    16861 gcatattgta cttttcctct tactcgactt aaccagtacc aacccagctt ctcaacggat
    16921 ttataccatg gcactttaaa gccagcatca ctgacaatga gcggtgtggt gttactcggt
    16981 agaatgctcg caaggtcggc tagaaattgg tcatgagctt tctttgaaca ttgctctgaa
    17041 agcgggaacg ctttctcata aagagtaaca gaacgaccgt gtagtgcgac tgaagctcgc
    17101 aataccataa gccgtttttg ctcacggata tcagaccagt caacaagtac aatgggcatc
    17161 gtattgcccg aacagataaa gctagcatgc caacggtata cagcgagtcg ctctttgtgg
    17221 aggtgacgat tacctaacaa tcggtcgatt cgtttgatgt tatgttttgt tctcgctttg
    17281 gttggcaggt tacggccaag ttcggtaaga gtgagagttt tacagtcaag taaggcgtgg
    17341 caagccaacg ttaagctgtt gagtcgtttt aagtgtaatt cggggcagaa ttggtaaaga
    17401 gagtcgtgta aaatatcgag ttcgcacatt ttgttgtctg attattgatt tttggcgaaa
    17461 ccatttgatc atatgacaag atgtgtatct accttaactt aatgattttg ataaaaatca
    17521 ttaggggatt catcagccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    17581 aaaagatcaa aggatcttc
//
