LOCUS       sequence-403976-       11468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mitochondrial directed jAspSnFR3-mRuby3 in lentiviral expression 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11468)
  AUTHORS   Davidsen K, Marvin JS, Aggarwal A, Brown TA, Sullivan LB
  TITLE     An engineered biosensor enables dynamic aspartate measurements in 
            living cells.
  JOURNAL   eLife. 2024 Feb 23;12:RP90024. doi: 10.7554/eLife.90024.
  PUBMED    38393319
REFERENCE   2  (bases 1 to 11468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2024-02-23"; volume: "12:RP90024. doi"; pages: " 
            10.7554/eLife.90024"
COMMENT     Sequence Label: pLenti-MTS-jAspSnFR3-mRuby3
FEATURES             Location/Qualifiers
     source          1..11468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(132..200,201..992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(350..369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1652..1671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1905..1922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2075..2080,2081..2098,2099..2105)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2118..2140
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2137..2153
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2172..2192
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2220..2446
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2447..2627
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2674..2799
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3292..3525
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3710..3754
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4021..4137
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4160..4463
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4464..4667
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4618..4638
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4664..4688
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    4772..4796
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4855..4864
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     6186..6207
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6310..6331)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             7549..7566
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(7591..7615)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7646..8234
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7699..7719)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8117..8128)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        8290..8789
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8325..8348)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8722..8741
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8807..9832
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8807..9832
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             10206..10439
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10511..10632
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10548..10567)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10602..10621
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10672..10807
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10827..10846)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10828..10846)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10856..10873)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10856..10872)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10865..10887)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11014..11442
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11101..11120)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11311..11332
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
     2461 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     2521 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     2581 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     2641 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     2701 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     2761 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     2821 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     2881 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     2941 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     3001 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     3061 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     3121 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     3181 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     3241 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     3301 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     3361 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     3421 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     3481 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     3541 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     3601 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     3661 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     3721 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     3781 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     3841 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     3901 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     3961 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcggttaac
     4021 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     4081 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc
     4141 gataagcttg ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     4201 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     4261 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     4321 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     4381 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     4441 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     4501 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     4561 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     4621 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
     4681 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgactct
     4741 agtccagtgt ggtggaattc tgcagatatc aacaagtttg tacaaaaaag caggctttaa
     4801 aggaaccaat tcagtcgact ggatccggta ccgaattcgc ttactaaaag ccaggccacc
     4861 atggcactgc ttcgcgccgc cgtttcagaa ctcagacgga gaggacgggg tgcgcttact
     4921 cccctcccgg cgctgtctag cttgctttcc tcacttagcc cccgaagtcc cgcctcaacg
     4981 cgcccagagc caaacaatcc acacgcagat cgacgccatg tcatcgcttt gaggcgatgc
     5041 cccccacttc ctgcctctgc cgttctggca cctgaactcc tgcatgcacg aggattgctc
     5101 ccgagacatt ggtctcatgc ctctcccttg tccacgtcct cttcatccag tagaccagca
     5161 gataaggcgc agttgacctg ggtcgataaa tggatcccag aagccgcgag accgtatggc
     5221 agatctgccg caggcagcac gctggacaaa atcgccaaaa acggtgtgat tgtcgtcggt
     5281 caccgtaaat ctgccgtgcc tttctcttat tacgacagtc agcacaaagt ggtgggttac
     5341 tcgcaggaat actccaacgc cattgttgaa gcagtgaaaa agaaactcaa caaaccggac
     5401 ttgcaggtaa aactgattcc gattacccca caaaaccgta ttccactgct gcaaaacggc
     5461 acttacgatt ttgaatgtgg ttctaccacc aacaacgtcg aacgccaaaa acaggcggct
     5521 ttctctgaca ctattttcgt ggtcggtacg cgcctgttga ccaaaaaggg tggcgatatc
     5581 aaagattttg ccaacctgaa agacaaagcc gtagtcgtca cttccggcac tacctctgaa
     5641 gttttgctca acaaactgaa tgaagagcaa aaaatgaata tgcgcatcat cagcgccaaa
     5701 gatcacggtg actctttccg caccctggaa agcggtcgtg ccgttgcctt tatgatggat
     5761 gacgtgctgc tggccggtga acgtgcgaaa gcgaagaaac cagacgactg ggaaatcgtc
     5821 ggcaagccgc agtctcagga ggcctacggt tgtatgttgc gtaaagatga tccgcagttc
     5881 aaaaagctga tggatgacac catcgctcag gtgcagacct ccggtgaagc ggaaaaatgg
     5941 tttgataagt ggttcaaaaa tccaattctg gtgagccaca tcgtctatat caccgccgac
     6001 aagcagaaga acggcatcaa ggcgaacttc aagatccacc acaacgtgga ggacggcagc
     6061 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     6121 cccgacaacc actacctgag ctatcagtcc gtgctgagca aagaccccaa cgagaagcgc
     6181 gatcacatgg tcctgctgga gttcgtgacc gccaccacga actctctcgg catggacgag
     6241 ctgtacaagg gcggtaccgg agggagcatg agcaagggcg aggagctgtt caccggggtg
     6301 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgcgcggc
     6361 gagggcgagg gcgatgccac caacggcaag ctaaccctga agctcatctg caccaccggc
     6421 aagctgcccg tgccctggcc caccctcgtg accaccctgg gctacggcgt gcagtgcttc
     6481 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     6541 tacgtccagg agcgcaccat cagcttcaag gacgacggaa cctacaagac ccgcgccgag
     6601 gtgaagttag agggcgacac cctggtgaac cgcatcgagc taaagggcat cgacttcaag
     6661 gaggacggca acatcctggg gcacaagctg gagtacaact ttaacaaccc cctgaacatg
     6721 aatttcgaac tgtcagacga aatgaaagca ctgttcaaag aaccgaatga caaggcactg
     6781 aaactgcagt ctaccggctc cggaaatgct gtgggccagg acacgcagga gcgtatggtg
     6841 tctaagggcg aagagctgat caaggaaaat atgcgtatga aggtggtcat ggaaggttcg
     6901 gtcaacggcc accaattcaa atgcacaggt gaaggagaag gcaggccgta cgagggagtg
     6961 caaaccatga ggatcaaagt catcgaggga ggacccctgc catttgcctt tgacattctt
     7021 gccacgtcgt tcatgtatgg cagccgtact tttatcaagt acccggcaga cattcctgat
     7081 ttctttaaac agtcctttcc tgagggtttt acttgggaaa gagttacgag atacgaagat
     7141 ggtggagtcg tcaccgtcac gcaggacacc agccttgagg atggcgaact cgtttacaac
     7201 gtcaaagtca gaggggtaaa ctttccctcc aatggtcccg tgatgcagaa gaagaccaag
     7261 ggttgggagc ctaatacaga gatgatgtat ccagcagatg gtggtctgag gggatacact
     7321 gatattgcac tgaaagttga tggtggtggc catctgcatt gcaatttcgt aacaacttac
     7381 aggtcaaaaa agaccgtcgg gaacatcaag atgcccggtg tccatgccgt tgatcaccgc
     7441 ctggaaagga tagaggaaag tgacaatgaa acgtacgtag tacaacgcga agtcgcagtt
     7501 gccaagtact ccaatcttgg tggtggtatg gatgaacttt ataagtccca tcaccatcac
     7561 caccattaag aattcgaagc ttatcgctag acccagcttt cttgtacaaa gtggttgata
     7621 tccagcacag tggcggccgc tcgacaatca acctctggat tacaaaattt gtgaaagatt
     7681 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     7741 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     7801 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     7861 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     7921 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     7981 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     8041 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     8101 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     8161 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     8221 ggccgcctcc ccgcctggaa ttctgcagat atccggacta gtgatctctc gaggttaacg
     8281 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
     8341 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca
     8401 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc
     8461 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
     8521 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg
     8581 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc
     8641 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc
     8701 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt
     8761 tctcctcttc ctcatctccg ggcctttcga cctgcatccc gccaccatga aaaagcctga
     8821 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct
     8881 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg
     8941 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg
     9001 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga
     9061 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga
     9121 aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc
     9181 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac
     9241 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt
     9301 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc
     9361 cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct
     9421 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc
     9481 ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca
     9541 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta
     9601 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga
     9661 tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg
     9721 gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact
     9781 cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat agagtagatg
     9841 ccgaccgaac aagagctgat ttcgagaacg cctcagccag caactcgcgc gagcctagca
     9901 aggcaaatgc gagagaacgg ccttacgctt ggtggcacag ttctcgtcca cagttcgcta
     9961 agctcgctcg gctgggtcgc gggagggccg gtcgcagtga ttcaggccct tctggattgt
    10021 gttggtcccc agggcacgat tgtcatgccc acgcactcgg gtgatctgac tgatcccgca
    10081 gattggagat cgccgcccgt gcctgccgat tgggtgcaga tccgtcgagg gcccggtacc
    10141 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
    10201 gggactggaa gggctagctc actcccaacg aagacaagat ctgctttttg cttgtactgg
    10261 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
    10321 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
    10381 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
    10441 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
    10501 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
    10561 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    10621 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
    10681 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
    10741 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
    10801 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
    10861 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
    10921 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
    10981 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
    11041 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
    11101 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
    11161 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
    11221 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
    11281 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
    11341 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
    11401 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
    11461 ttacaatt
//
