LOCUS       sequence-403984-       14117 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RNA editing in plants. Plasmid contains extradiol-dADARcd-LIC 
            cassette-dADARcd-35S-hptII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14117)
  AUTHORS   Zhou G, Niu R, Zhou Y, Luo M, Peng Y, Wang H, Wang Z, Xu G
  TITLE     Proximity editing to identify RNAs in phase-separated RNA binding 
            protein condensates.
  JOURNAL   Cell Discov. 2021 Aug 24;7(1):72. doi: 10.1038/s41421-021-00288-9.
  PUBMED    34426573
REFERENCE   2  (bases 1 to 14117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-08-24"; volume: "7(1):72. doi"; pages: " 
            10.1038/s41421-021-00288-9"
COMMENT     Sequence Label: pZG164
FEATURES             Location/Qualifiers
     source          1..14117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        216..262
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             291..542
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
     CDS             558..791
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             801..1736
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             849..1742
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     promoter        2300..2479
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2332..2515
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      3630..3882
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        4203..4249
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(7318..7335)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7318..7334)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7327..7349)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    7556..7580
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8101..8892
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9138..9726
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9627..9646
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9880..9897
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9912..10052
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9978..9997)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10138..10160
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10396..10590
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10656..11729)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12158..12787)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc taagcttgca tgcctgcagg atatcgtgga tccaagcttg
      121 ccacgtgccg ccacgtgccg ccacgtgccg ccacgtgcct ctagaggatc catctccact
      181 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
      241 agttcatttc atttggagag gacacgctgg gatccccaat tccgggcgga atgaaagcgt
      301 taacggccag gcaacaagag gtgtttgatc tcatccgtga tcacatcagc cagacaggta
      361 tgccgccgac gcgtgcggaa atcgcgcagc gtttggggtt ccgttcccca aacgcggctg
      421 aagaacatct gaaggcgctg gcacgcaaag gcgttattga aattgtttcc ggcgcatcac
      481 gcgggattcg tctgttgcag gaagaggaag aagggttgcc gctggtaggt cgtgtggctg
      541 ccggtgaacc gtcgagcgcc cccccgaccg atgtcagcct gggggacgag ctccacttag
      601 acggcgagga cgtggcgatg gcgcatgccg acgcgctaga cgatttcgat ctggacatgt
      661 tgggggacgg ggattccccg ggtccgggat ttacccccca cgactccgcc ccctacggcg
      721 ctctggatat ggccgacttc gagtttgagc agatgtttac cgatgccctt ggaattgacg
      781 agtacggtgg ggatccgtct gctggagaca tgagagctgc caacctttgg ccaagcccgc
      841 tcatgatcaa acgctctaag aagaacagcc tggccttgtc cctgacggcc gaccagatgg
      901 tcagtgcctt gttggatgct gagcccccca tactctattc cgagtatgat cctaccagac
      961 ccttcagtga agcttcgatg atgggcttac tgaccaacct ggcagacagg gagctggttc
     1021 acatgatcaa ctgggcgaag agggtgccag gctttgtgga tttgaccctc catgatcagg
     1081 tccaccttct agaatgtgcc tggctagaga tcctgatgat tggtctcgtc tggcgctcca
     1141 tggagcaccc agggaagcta ctgtttgctc ctaacttgct cttggacagg aaccagggaa
     1201 aatgtgtaga gggcatggtg gagatcttcg acatgctgct ggctacatca tctcggttcc
     1261 gcatgatgaa tctgcaggga gaggagtttg tgtgcctcaa atctattatt ttgcttaatt
     1321 ctggagtgta cacatttctg tccagcaccc tgaagtctct ggaagagaag gaccatatcc
     1381 accgagtcct ggacaagatc acagacactt tgatccacct gatggccaag gcaggcctga
     1441 ccctgcagca gcagcaccag cggctggccc agctcctcct catcctctcc cacatcaggc
     1501 acatgagtaa caaaggcatg gagcatctgt acagcatgaa gtgcaagaac gtggtgcccc
     1561 tctatgacct gctgctggag atgctggacg cccaccgcct acatgcgccc actagccgtg
     1621 gaggggcatc cgtggaggag acggaccaaa gccacttggc cactgcgggc tctacttcat
     1681 cgcattcctt gcaaaagtat tacatcacgg gggaggcaga gggtttccct gccacagtct
     1741 gagagctccc tggcggaatt cccagagatg ttagctgaaa tcatcactaa tcagatacca
     1801 aaatattcaa atggaaatat caaaaagctt ctgtttcatc aaaaatgact cgacctaact
     1861 gagtaagcta gcttgttcga gtattatggc attgggaaaa ctgtttttct tgtaccattt
     1921 gttgtgcttg taatttactg tgttttttat tcggttttcg ctatcgaact gtgaaatgga
     1981 aatggatgga gaagagttaa tgaatgatat ggtccttttg ttcattctca aattaatatt
     2041 atttgttttt tctcttattt gttgtgtgtt gaatttgaaa ttataagaga tatgcaaaca
     2101 ttttgttttg agtaaaaatg tgtcaaatcg tggcctctaa tgaccgaagt taatatgagg
     2161 agtaaaacac tagatcccca aacaagcttg aaactgaagg cgggaaacga caatctgatc
     2221 atgagcggag aattaaggga gtcacgttat gacccccgcc gatgacgcgg gacaagccgt
     2281 tttacgtttg gaactgacag aaccgcaacg ttgaaggagc cactcagccg cgggtttctg
     2341 gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa aagtcgccta
     2401 aggtcactat cagctagcaa atatttcttg tcaaaaatgc tccactgacg ttccataaat
     2461 tcccctcggt atccaattag agtctcatat tcactctcaa tccaaataat ctgcaccgga
     2521 tccgctagag gatctcgacc tgcaagatcc cggggggcaa tgagatatga aaaagcctga
     2581 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct
     2641 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg
     2701 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg
     2761 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga
     2821 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga
     2881 aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc
     2941 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac
     3001 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt
     3061 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc
     3121 cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct
     3181 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc
     3241 ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca
     3301 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta
     3361 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga
     3421 tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg
     3481 gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact
     3541 cgccgatagt ggaaaccgac gccccagcac tcgtccggga tcttggaggt gatgtaacat
     3601 gatcacaagc tgatcccccg aatttccccg atcgttcaaa catttggcaa taaagtttct
     3661 taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg
     3721 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
     3781 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
     3841 aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg atcccccctc
     3901 gacagcttgc atgccgcttg ggctgcaggt cgaggctaaa aaactaatcg cattatcatc
     3961 ccctcgacgt actgtacata taaccactgg ttttatatac agcagtactg tacatataac
     4021 cactggtttt atatacagca gtcgacgtac tgtacatata accactggtt ttatatacag
     4081 cagtactgta catataacca ctggttttat atacagcagt cgaggtaaga ttagatatgg
     4141 atatgtatat ggatatgtat atggtggtaa tgccatgtaa tatgctcgac tctaggatct
     4201 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac acgctgaagc
     4261 tagtcgactc tagcctcgag accatgtact ctcctatggt cgtaccgcaa aaaaatgtgc
     4321 ctctccctat agacgataaa tctagctcca tggagcttcc gcagatccac gcagacacta
     4381 tcggtcgatt ggtgctggaa aagttcatgg aggttattaa aggtcaggag gcttatagcc
     4441 gtcgaaaagt tttggctgga atcgtaatga ccgaaaacat gaacttctgt gaggcaaaag
     4501 tcatttcagt gtccacgggc accaagtgtg tgtccggcga gcatatgtcc gtaaacggtg
     4561 cggtactcaa tgacagccac gccgagatag tgtcacgtcg ttgtctcctc aagtatctct
     4621 atgcccaact ggatcttcaa tgcaaccagg cgaccgctta tcagagcata tttgtgcgta
     4681 atactgacgg tcagtatccc tataaactga agtctggggt ccattttcat ctctatataa
     4741 acacggcgcc ctgcggcgat gcccgtattt tttcacctca cgagaatgac acgggagtag
     4801 ataaacatcc caaccgaaaa gcaagagggc agctccgaac caaaatcgaa tccggccagg
     4861 gcacaatccc agtgaaatct agcgacggca ttcaaacttg ggacggcgta ctacaaggcc
     4921 agcgtttgct tacgatgtcc tgctccgata aaattgctag gtggaacatc gtcggtatcc
     4981 agggctcctt gctctcttca attatagagc ccgtctatct gcattcaatt gtcttagggt
     5041 cactcttgca tcctgaacat atgtaccgag ccgtttgtgg gagaatagaa aagagtatcc
     5101 agggtctacc cccaccttac catttgaaca aacccagact ggcattggtc acatccgcag
     5161 aaccacgtaa ccaagccaag gccccgaatt ttggaataaa ctggactata ggtgacacgg
     5221 aactagaggt tgtgaactct ctcacaggca gaaccatagg agggcaagtc agccgtataa
     5281 cgaagcaggc ttttttcgta aagtatggtt tcttgatggc taatttgccc ggaatcctcg
     5341 tccgaaaggt aacgaccgat tacggtcaga caaaagcgaa tgtaaaggat taccagatcg
     5401 cgaagttgga attgttctca gccttcaaga gggaggatct tggatcatgg ctaaagaaac
     5461 caatagagca ggacgagttt ggattggctg aagctcgagt accttcgacg acaagaccgg
     5521 gcccggctct tctcctcact cgaccagatc tcgtacgcgt cccggggcgg tggctcatct
     5581 ggcggaggtg gatcctactc tcctatggtc gtaccgcaaa aaaatgtgcc tctccctata
     5641 gacgataaat ctagctccat ggagcttccg cagatccacg cagacactat cggtcgattg
     5701 gtgctggaaa agttcatgga ggttattaaa ggtcaggagg cttatagccg tcgaaaagtt
     5761 ttggctggaa tcgtaatgac cgaaaacatg aacttctgtg aggcaaaagt catttcagtg
     5821 tccacgggca ccaagtgtgt gtccggcgag catatgtccg taaacggtgc ggtactcaat
     5881 gacagccacg ccgagatagt gtcacgtcgt tgtctcctca agtatctcta tgcccaactg
     5941 gatcttcaat gcaaccaggc gaccgcttat cagagcatat ttgtgcgtaa tactgacggt
     6001 cagtatccct ataaactgaa gtctggggtc cattttcatc tctatataaa cacggcgccc
     6061 tgcggcgatg cccgtatttt ttcacctcac gagaatgaca cgggagtaga taaacatccc
     6121 aaccgaaaag caagagggca gctccgaacc aaaatcgaat ccggccaggg cacaatccca
     6181 gtgaaatcta gcgacggcat tcaaacttgg gacggcgtac tacaaggcca gcgtttgctt
     6241 acgatgtcct gctccgataa aattgctagg tggaacatcg tcggtatcca gggctccttg
     6301 ctctcttcaa ttatagagcc cgtctatctg cattcaattg tcttagggtc actcttgcat
     6361 cctgaacata tgtaccgagc cgtttgtggg agaatagaaa agagtatcca gggtctaccc
     6421 ccaccttacc atttgaacaa acccagactg gcattggtca catccgcaga accacgtaac
     6481 caagccaagg ccccgaattt tggaataaac tggactatag gtgacacgga actagaggtt
     6541 gtgaactctc tcacaggcag aaccatagga gggcaagtca gccgtataac gaagcaggct
     6601 tttttcgtaa agtatggttt cttgatggct aatttgcccg gaatcctcgt ccgaaaggta
     6661 acgaccgatt acggtcagac aaaagcgaat gtaaaggatt accagatcgc gaagttggaa
     6721 ttgttctcag ccttcaagag ggaggatctt ggatcatggc taaagaaacc aatagagcag
     6781 gacgagtttg gattggctga atgaactagt cgatccaggc ctcccagctt tcgtccgtat
     6841 catcggtttc gacaacgttc gtcaagttca atgcatcagt ttcattgccc acacaccaga
     6901 atcctactaa gtttgagtat tatggcattg gaaaagctgt tttcttctat catttgttct
     6961 gcttgtaatt tactgtgttc tttcagtttt tgttttcgga catcaaaatg caaatggatg
     7021 gataagagtt aataaatgat atggtccttt tgttcattct caaattatta ttatctgttg
     7081 tttttacttt aatgggttga atttaagtaa gaaaggaact aacagtgtga tattaaggtg
     7141 caatgttaga catataaaac agtctttcac ctctctttgg ttatgtcttg aattggtttg
     7201 tttcttcact tatctgtgta atcaagttta ctatgagtct atgatcaagt aattatgcaa
     7261 tcaagttaag tacagtatag gctttttgtg tcgagggggt accgagtcga ggaattcact
     7321 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     7381 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     7441 ttcccaacag ttgcgcagcc tgaatggcgg gtaccgagct cgaattcaat tcggcgttaa
     7501 ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgttta
     7561 caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag ctcggcacaa
     7621 aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg agagccgttg
     7681 taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc
     7741 cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag
     7801 cgttgctgcc tgtgatcaat tcgggcacga acccagtgga cataagcctg ttcggttcgt
     7861 aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc
     7921 agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta
     7981 cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg
     8041 ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc
     8101 atgggggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc
     8161 gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc
     8221 ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa
     8281 acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc
     8341 gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt
     8401 tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt
     8461 atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa
     8521 catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag
     8581 gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct
     8641 ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc
     8701 aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat
     8761 cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc
     8821 tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta
     8881 gtcggcaaat aatgtctagc tagaaattcg ttcaagccga cgccgcttcg cggcgcggct
     8941 taactcaagc gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag
     9001 tctgctttta ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca
     9061 tgcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     9121 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     9181 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     9241 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     9301 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     9361 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     9421 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     9481 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     9541 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     9601 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     9661 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     9721 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     9781 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     9841 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     9901 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     9961 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc
    10021 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
    10081 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
    10141 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagggtgcct
    10201 tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc tggtagattg
    10261 cctggccgta ggccagccat ttttgagcgg ccagcggccg cgataggccg acgcgaagcg
    10321 gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg
    10381 cgctggccag acagttatgc acaggccagg cgggttttaa gagttttaat aagttttaaa
    10441 gagttttagg cggaaaaatc gccttttttc tcttttatat cagtcactta catgtgtgac
    10501 cggttcccaa tgtacggctt tgggttccca atgtacgggt tccggttccc aatgtacggc
    10561 tttgggttcc caatgtacgt gctatccaca ggaaagagac cttttcgacc tttttcccct
    10621 gctagggcaa tttgccctag catctgctcc gtacattagg aaccggcgga tgcttcgccc
    10681 tcgatcaggt tgcggtagcg catgactagg atcgggccag cctgccccgc ctcctccttc
    10741 aaatcgtact ccggcaggtc atttgacccg atcagcttgc gcacggtgaa acagaacttc
    10801 ttgaactctc cggcgctgcc actgcgttcg tagatcgtct tgaacaacca tctggcttct
    10861 gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc
    10921 aaaaagtaat cggggtgaac cgtcagcacg tccgggttct tgccttctgt gatctcgcgg
    10981 tacatccaat cagctagctc gatctcgatg tactccggcc gcccggtttc gctctttacg
    11041 atcttgtagc ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg gccgttcttg
    11101 gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta accgaatgca ggtttctacc
    11161 aggtcgtctt tctgctttcc gccatcggct cgccggcaga acttgagtac gtccgcaacg
    11221 tgtggacgga acacgcggcc gggcttgtct cccttccctt cccggtatcg gttcatggat
    11281 tcggttagat gggaaaccgc catcagtacc aggtcgtaat cccacacact ggccatgccg
    11341 gccggccctg cggaaacctc tacgtgcccg tctggaagct cgtagcggat cacctcgcca
    11401 gctcgtcggt cacgcttcga cagacggaaa acggccacgt ccatgatgct gcgactatcg
    11461 cgggtgccca cgtcatagag catcggaacg aaaaaatctg gttgctcgtc gcccttgggc
    11521 ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc gggattcttt gcggattcga
    11581 tcagcggccg cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg
    11641 gcggcctgcg cggccttcaa cttctccacc aggtcatcac ccagcgccgc gccgatttgt
    11701 accgggccgg atggtttgcg accgctcacg ccgattcctc gggcttgggg gttccagtgc
    11761 cattgcaggg ccggcagaca acccagccgc ttacgcctgg ccaaccgccc gttcctccac
    11821 acatggggca ttccacggcg tcggtgcctg gttgttcttg attttccatg ccgcctcctt
    11881 tagccgctaa aattcatcta ctcatttatt catttgctca tttactctgg tagctgcgcg
    11941 atgtattcag atagcagctc ggtaatggtc ttgccttggc gtaccgcgta catcttcagc
    12001 ttggtgtgat cctccgccgg caactgaaag ttgacccgct tcatggctgg cgtgtctgcc
    12061 aggctggcca acgttgcagc cttgctgctg cgtgcgctcg gacggccggc acttagcgtg
    12121 tttgtgcttt tgctcatttt ctctttacct cattaactca aatgagtttt gatttaattt
    12181 cagcggccag cgcctggacc tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg
    12241 ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa
    12301 tgggcagctc gtacccggcc agcgcctcgg caacctcacc gccgatgcgc gtgcctttga
    12361 tcgcccgcga cacgacaaag gccgcttgta gccttccatc cgtgacctca atgcgctgct
    12421 taaccagctc caccaggtcg gcggtggccc atatgtcgta agggcttggc tgcaccggaa
    12481 tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc
    12541 cgtcgatcac tacgaagtcg cgccggccga tggccttcac gtcgcggtca atcgtcgggc
    12601 ggtcgatgcc gacaacggtt agcggttgat cttcccgcac ggccgcccaa tcgcgggcac
    12661 tgccctgggg atcggaatcg actaacagaa catcggcccc ggcgagttgc agggcgcggg
    12721 ctagatgggt tgcgatggtc gtcttgcctg acccgccttt ctggttaagt acagcgataa
    12781 ccttcatgcg ttccccttgc gtatttgttt atttactcat cgcatcatat acgcagcgac
    12841 cgcatgacgc aagctgtttt actcaaatac acatcacctt tttagacggc ggcgctcggt
    12901 ttcttcagcg gccaagctgg ccggccaggc cgccagcttg gcatcagaca aaccggccag
    12961 gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt acccggccgc
    13021 gatcatctcc gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc cgtcctggtg
    13081 cggtttcatg cttgttcctc ttggcgttca ttctcggcgg ccgccagggc gtcggcctcg
    13141 gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt cacttcctcg
    13201 ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc agccgcctct
    13261 ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg tgccggggtg
    13321 agggtagggc gggggccaaa cttcacgcct cgggccttgg cggcctcgcg cccgctccgg
    13381 gtgcggtcga tgattaggga acgctcgaac tcggcaatgc cggcgaacac ggtcaacacc
    13441 atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg caggcccgcg
    13501 ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg ggccaggcgg
    13561 tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat cctggccagc
    13621 tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa acagcttggt gcagccggcc
    13681 gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt cggtgctgac gcgggcatag
    13741 cccagcaggc cagcggcggc gctcttgttc atggcgtaat gtctccggtt ctagtcgcaa
    13801 gtattctact ttatgcgact aaaacacgcg acaagaaaac gccaggaaaa gggcagggcg
    13861 gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt tttcagaaga cggctgcact
    13921 gaacgtcaga agccgactgc actatagcag cggaggggtt ggatcaaagt actttaaagt
    13981 actttaaagt actttaaagt actttgatcc cgaggggaac cctgtggttg gcatgcacat
    14041 acaaatggac gaacggataa accttttcac gcccttttaa atatccgatt attctaataa
    14101 acgctctttt ctcttag
//
