LOCUS       sequence-403996-       12906 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RNA editing in plants. Plasmid contains 
            35S-dADARcd-LIC1-ccdB-LIC2-35S-bar.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12906)
  AUTHORS   Zhou G, Niu R, Zhou Y, Luo M, Peng Y, Wang H, Wang Z, Xu G
  TITLE     Proximity editing to identify RNAs in phase-separated RNA binding 
            protein condensates.
  JOURNAL   Cell Discov. 2021 Aug 24;7(1):72. doi: 10.1038/s41421-021-00288-9.
  PUBMED    34426573
REFERENCE   2  (bases 1 to 12906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-08-24"; volume: "7(1):72. doi"; pages: " 
            10.1038/s41421-021-00288-9"
COMMENT     Sequence Label: pZG165
FEATURES             Location/Qualifiers
     source          1..12906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        501..846
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    2154..2278
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2303..2308,2309..2326,2327..2333)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2387..3046
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2453..2472)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3388..3693
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3572..3591
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3734..3858)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3893..4534
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     primer_bind     complement(4542..4559)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4542..4558)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4551..4573)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    4761..4785
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6086..6715
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7144..8217
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8283..8477
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8713..8735)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8821..8961
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8876..8895
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8976..8993)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9147..9735)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9227..9246)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9822..10616)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11041..11065
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    11143..11317
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(11324..11875)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(11917..12593)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    12784..12805
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12820..12825,12826..12843,12844..12850)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12858..12874
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12863..12885
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12882..12898
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12882..12898
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gaattcggtc cccagattag ccttttcaat ttcagaaaga atgctaaccc acagatggtt
       61 agagaggctt acgcagcagg tctcatcaag acgatctacc cgagcaataa tctccaggaa
      121 atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taactgcatc
      181 aagaacacag agaaagatat atttctcaag atcagaagta ctattccagt atggacgatt
      241 caaggcttgc ttcacaaacc aaggcaagta atagagattg gagtctctaa aaaggtagtt
      301 cccactgaat caaaggccat ggagtcaaag attcaaatag aggacctaac agaactcgcc
      361 gtaaagactg gcgaacagtt catacagagt ctcttacgac tcaatgacaa gaagaaaatc
      421 ttcgtcaaca tggtggagca cgacacactt gtctactcca aaaatatcaa agatacagtc
      481 tcagaagacc aaagggcaat tgagactttt caacaaaggg taatatccgg aaacctcctc
      541 ggattccatt gcccagctat ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc
      601 tcctacaaat gccatcattg cgataaagga aaggccatcg ttgaagatgc ctctgccgac
      661 agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca
      721 accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca
      781 caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag
      841 agaacacggg ggacgagctc accatgtact ctcctatggt cgtaccgcaa aaaaatgtgc
      901 ctctccctat agacgataaa tctagctcca tggagcttcc gcagatccac gcagacacta
      961 tcggtcgatt ggtgctggaa aagttcatgg aggttattaa aggtcaggag gcttatagcc
     1021 gtcgaaaagt tttggctgga atcgtaatga ccgaaaacat gaacttctgt gaggcaaaag
     1081 tcatttcagt gtccacgggc accaagtgtg tgtccggcga gcatatgtcc gtaaacggtg
     1141 cggtactcaa tgacagccac gccgagatag tgtcacgtcg ttgtctcctc aagtatctct
     1201 atgcccaact ggatcttcaa tgcaaccagg cgaccgctta tcagagcata tttgtgcgta
     1261 atactgacgg tcagtatccc tataaactga agtctggggt ccattttcat ctctatataa
     1321 acacggcgcc ctgcggcgat gcccgtattt tttcacctca cgagaatgac acgggagtag
     1381 ataaacatcc caaccgaaaa gcaagagggc agctccgaac caaaatcgaa tccggccagg
     1441 gcacaatccc agtgaaatct agcgacggca ttcaaacttg ggacggcgta ctacaaggcc
     1501 agcgtttgct tacgatgtcc tgctccgata aaattgctag gtggaacatc gtcggtatcc
     1561 agggctcctt gctctcttca attatagagc ccgtctatct gcattcaatt gtcttagggt
     1621 cactcttgca tcctgaacat atgtaccgag ccgtttgtgg gagaatagaa aagagtatcc
     1681 agggtctacc cccaccttac catttgaaca aacccagact ggcattggtc acatccgcag
     1741 aaccacgtaa ccaagccaag gccccgaatt ttggaataaa ctggactata ggtgacacgg
     1801 aactagaggt tgtgaactct ctcacaggca gaaccatagg agggcaagtc agccgtataa
     1861 cgaagcaggc ttttttcgta aagtatggtt tcttgatggc taatttgccc ggaatcctcg
     1921 tccgaaaggt aacgaccgat tacggtcaga caaaagcgaa tgtaaaggat taccagatcg
     1981 cgaagttgga attgttctca gccttcaaga gggaggatct tggatcatgg ctaaagaaac
     2041 caatagagca ggacgagttt ggattggctg aatctagagg aggtggctca tctggcggag
     2101 gtcagatctc gtacgcgtcc cggggcggta ccttcgacga caagaccggg cccacaagtt
     2161 tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga
     2221 ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag tcactatggc
     2281 ggccgcatta ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat
     2341 tttgagttag gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat
     2401 cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt
     2461 tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt
     2521 aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg
     2581 cctgatgaat gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg
     2641 ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct
     2701 ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc
     2761 gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt
     2821 ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa
     2881 cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat
     2941 gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct
     3001 taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaaagat ctggatccgg
     3061 cttactaaaa gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat
     3121 atatactgat atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt
     3181 acagtgacag ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct
     3241 ccggtctggt aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga
     3301 aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt
     3361 ttgctgacga gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag
     3421 agccgttatc gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg
     3481 atggtgatcc ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac
     3541 ccggtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg
     3601 ccggtctccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa
     3661 aacgccatta acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag
     3721 tctgcaggtc gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt
     3781 tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc
     3841 tttcttgtac aaagtggtag gggcccggct cttctcctca ctcgacctgc agagctttcg
     3901 ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca
     3961 caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca
     4021 tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat
     4081 ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat
     4141 attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa
     4201 acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg
     4261 aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt
     4321 cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca
     4381 tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt
     4441 ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt
     4501 tatgacttgc caattgattg acaacatgca tcaagcttgg cactggccgt cgttttacaa
     4561 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     4621 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     4681 agcctgaatg gcgaatgcta gagcagcttg agcttggatc agattgtcgt ttcccgcctt
     4741 cagtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc
     4801 gtttattaga ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt
     4861 gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttgat ccaacccctc
     4921 cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga aaacgacatg
     4981 tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg gcgttttctt
     5041 gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag acattacgcc
     5101 atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac cgacgaccag
     5161 gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct gttttccgag
     5221 aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga ccacctacgc
     5281 cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta
     5341 ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg
     5401 tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc cggcattgcc
     5461 gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc
     5521 cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc
     5581 gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat
     5641 cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac cgaggccagg
     5701 cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc ggccgccgag
     5761 aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac gaaccgtttt
     5821 tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc gagccgcccg
     5881 cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg
     5941 cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat
     6001 gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa
     6061 ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg
     6121 gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg ccggggccga
     6181 tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg ccgtgcggga
     6241 agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc
     6301 catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg acttggctgt
     6361 gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc cttacgacat
     6421 atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca cggatggaag
     6481 gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt
     6541 tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca cgcagcgcgt
     6601 gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac ccgagggcga
     6661 cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca tttgagttaa
     6721 tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg tccgagcgca
     6781 cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc
     6841 aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc
     6901 aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta aatgagcaaa
     6961 tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca
     7021 accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt ggccaggcgt
     7081 aagcggctgg gttgcctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc
     7141 ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac
     7201 ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca
     7261 cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg
     7321 ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt
     7381 ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc
     7441 gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca
     7501 gacgggcacg tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg ggattacgac
     7561 ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag
     7621 ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg
     7681 cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc
     7741 acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc
     7801 gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag
     7861 tacatcgaga tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg
     7921 gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc
     7981 taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc
     8041 tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg
     8101 atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg
     8161 atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt
     8221 acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggtctcttt
     8281 cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac
     8341 attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa
     8401 gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc
     8461 cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct
     8521 acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct
     8581 ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg
     8641 ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga
     8701 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     8761 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     8821 cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca
     8881 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     8941 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     9001 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     9061 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     9121 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     9181 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     9241 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     9301 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     9361 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     9421 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     9481 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     9541 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     9601 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     9661 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     9721 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     9781 gaaaactcac gttaagggat tttggtcatg cattctaggt actaaaacaa ttcatccagt
     9841 aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc
     9901 tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca
     9961 taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac tttgccatct
    10021 ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc
    10081 ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt gtcttcttcc
    10141 cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa ttcggctaag
    10201 cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg aaagagcctg
    10261 atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc atactcttcc
    10321 gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc atgccgttca
    10381 aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat gtccttttcc
    10441 cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa atataggttt
    10501 tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt atcttttacg
    10561 cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt agccatttat
    10621 tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat tataacaaga
    10681 cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt
    10741 caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccgc
    10801 ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga
    10861 tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat
    10921 gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct
    10981 gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg
    11041 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
    11101 gacgttttta atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact
    11161 ggattttggt tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac
    11221 atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc
    11281 cttatctggg aactactcac acattattat ggagaaactc gagttagatc tcggtgacgg
    11341 gcaggaccgg acggggcggt accggcaggc tgaagtccag ctgccagaaa cccacgtcat
    11401 gccagttccc gtgcttgaag ccggccgccc gcagcatgcc gcggggggca tatccgagcg
    11461 cctcgtgcat gcgcacgctc gggtcgttgg gcagcccgat gacagcgacc acgctcttga
    11521 agccctgtgc ctccagggac ttcagcaggt gggtgtagag cgtggagccc agtcccgtcc
    11581 gctggtggcg gggggagacg tacacggtcg actcggccgt ccagtcgtag gcgttgcgtg
    11641 ccttccaggg tcccgcgtag gcgatgccgg cgacctcgcc gtccacctcg gcgacgagcc
    11701 agggatagcg ctcccgcaga cggacgaggt cgtccgtcca ctcctgcggt tcctgcggct
    11761 cggtacggaa gttgaccgtg cttgtctcga tgtagtggtt gacgatggtg cagaccgccg
    11821 gcatgtccgc ctcggtggca cggcggatgt cggccgggcg tcgttctggg ctcatggtct
    11881 cgagagagat agatttgtag agagagactg gtgatttcag cgtgtcctct ccaaatgaaa
    11941 tgaacttcct tatatagagg aagggtcttg cgaaggatag tgggattgtg cgtcatccct
    12001 tacgtcagtg gagatatcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc
    12061 tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc
    12121 atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc caccttcctt
    12181 ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga ggaggtttcc
    12241 cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga ctgtatcttt
    12301 gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttcacatcaa tccacttgct
    12361 ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat
    12421 ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat
    12481 ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg
    12541 ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc
    12601 tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca
    12661 ccatgttggc aagctgctct agccaatacg caaaccgcct ctccccgcgc gttggccgat
    12721 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
    12781 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
    12841 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat
    12901 gattac
//
