LOCUS       sequence-404005-       10224 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Heterologous expression in mammalian cell lines or Xenopus oocyte 
            (T7 RNA pol).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10224)
  AUTHORS   Wei AD, Wakenight P, Zwingman TA, Bard AM, Sahai N, Willemsen MH, 
            Schelhaas HJ, Stegmann APA, Verhoeven JS, de Man SA, Wessels MW, 
            Kleefstra T, Shinde DN, Helbig KL, Basinger A, Wagner VF, 
            Rodriguez-Buritica D, Bryant E, Millichap JJ, Millen KJ, Dobyns WB, 
            Ramirez JM, Kalume FK
  TITLE     Human KCNQ5 de novo mutations underlie epilepsy and intellectual 
            disability.
  JOURNAL   J Neurophysiol. 2022 Jul 1;128(1):40-61. doi: 10.1152/jn.00509.2021.
            Epub 2022 May 18.
  PUBMED    35583973
REFERENCE   2  (bases 1 to 10224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1152/jn.00509.2021"; journalName: "J Neurophysiol"; date: 
            "2022-07-1- 1"; volume: "128"; issue: "1"; pages: "40-61"
COMMENT     Sequence Label: hKCNQ5(WT).ires.mScarlet-pcDNA3
FEATURES             Location/Qualifiers
     source          1..10224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(152..169)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     379..398
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      579..588
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1305..1323)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1306..1323)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1343..1360)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1349..1573
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1619..2047
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1706..1725)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1916..1937
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2056..2076)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2061..2390
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2241..2376
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2303..2322
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2457..3251
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2511..2530)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3121..3140
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3425..3546
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3462..3481)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3516..3535
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3595..3611)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3595..3611)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3608..3630)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3619..3635
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3643..3649,3650..3667,3668..3673))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3688..3709
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3826..3843)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3997..4585)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4077..4096)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4756..5547,5548..5616))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5379..5398
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5617..5721)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5796..5815)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5987..6366
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6367..6570
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6521..6541
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6615..6634
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6615..6633
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           complement(6672..6714)
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     regulatory      6776..6785
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(10209..10224,1..559,560..562,563..571)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
ORIGIN
        1 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
       61 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
      121 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
      181 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
      241 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
      301 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
      361 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
      421 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
      481 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
      541 tgaaaaacac gatgataata tggccacaac ccgcggccgc caccatggtc tccaaaggag
      601 aagcggtcat taaagagttc atgaggttca aggttcatat ggaaggctcc atgaatggtc
      661 atgagttcga gattgaaggg gagggtgagg ggagacctta tgagggcact cagacagcga
      721 aattgaaggt gacaaaggga ggacctctcc cgttcagttg ggacatattg tcaccgcaat
      781 ttatgtatgg ttctagagcc ttcactaagc accctgccga catcccagat tactacaagc
      841 aatccttccc tgagggcttt aagtgggaga gagtaatgaa ttttgaagat ggcggggcag
      901 tcacagtaac acaagataca tccctggaag atggaacact tatctacaaa gttaagctca
      961 gaggaacgaa ttttccaccg gacggtccag tgatgcaaaa aaaaacaatg ggttgggaag
     1021 catctacaga gcgactgtac cctgaagacg gtgtgctgaa gggggacatc aaaatggccc
     1081 tgcgacttaa ggatggaggg cgctatttgg cagatttcaa gactacttac aaagccaaaa
     1141 agcctgtaca aatgcctgga gcttacaacg tggataggaa gcttgatatt accagtcaca
     1201 atgaagatta tacagtggta gaacaatatg aacgctcaga aggtcgccac agcactggag
     1261 gcatggatga gttgtacaag taggctagca ctagagggcc ctattctata gtgtcaccta
     1321 aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     1381 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     1441 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     1501 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     1561 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     1621 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     1681 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     1741 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     1801 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     1861 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     1921 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     1981 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     2041 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     2101 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     2161 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     2221 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     2281 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     2341 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     2401 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga
     2461 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     2521 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     2581 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     2641 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     2701 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     2761 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     2821 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     2881 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     2941 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     3001 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     3061 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     3121 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     3181 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     3241 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     3301 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     3361 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     3421 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     3481 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     3541 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     3601 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     3661 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     3721 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     3781 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     3841 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     3901 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     3961 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     4021 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     4081 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     4141 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     4201 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     4261 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     4321 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     4381 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     4441 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     4501 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4561 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4621 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     4681 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     4741 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     4801 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     4861 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     4921 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     4981 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     5041 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     5101 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     5161 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     5221 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     5281 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     5341 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     5401 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     5461 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     5521 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     5581 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     5641 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     5701 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     5761 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
     5821 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
     5881 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
     5941 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
     6001 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     6061 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     6121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     6181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     6241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     6301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     6361 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     6421 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     6481 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     6541 gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc cactgcttac
     6601 tggcttatcg aaattaatac gactcactat agggagaccc aagcttatcg ataccgtcga
     6661 cgctagctct gccaaagttg agcgtttatt ctgagcttct gcaaaaagaa caagaagggt
     6721 accgagctcg gatccactag taacggccgc cagtgtgctg gaattctgca gatatccatg
     6781 gcggccgccg ccgccatgcc ccgccaccac gcgggaggag aggagggcgg cgccgccggg
     6841 ctctgggtga agagcggcgc agcggcggcg gcggcgggcg gggggcgctt gggcagcggc
     6901 atgaaggatg tggagtcggg ccggggcagg gtgctgctga actcggcagc cgccaggggc
     6961 gacggcctgc tactgctggg cacccgcgcg gccacgcttg gtggcggcgg cggtggcctg
     7021 agggagagcc gccggggcaa gcagggggcc cggatgagcc tgctggggaa gccgctctct
     7081 tacacgagta gccagagctg ccggcgcaac gtcaagtacc ggcgggtgca gaactacctg
     7141 tacaacgtgc tggagagacc ccgcggctgg gcgttcatct accacgcttt cgtttttctc
     7201 cttgtctttg gttgcttgat tttgtcagtg ttttctacca tccctgagca cacaaaattg
     7261 gcctcaagtt gcctcttgat cctggagttc gtgatgattg tcgtctttgg tttggagttc
     7321 atcattcgaa tctggtctgc gggttgctgt tgtcgatata gaggatggca aggaagactg
     7381 aggtttgctc gaaagccctt ctgtgttata gataccattg ttcttatcgc ttcaatagca
     7441 gttgtttctg caaaaactca gggtaatatt tttgccacgt ctgcactcag aagtctccgt
     7501 ttcctacaga tcctccgcat ggtgcgcatg gaccgaaggg gaggcacttg gaaattactg
     7561 ggttcagtgg tttatgctca cagcaaggaa ttaatcacag cttggtacat aggatttttg
     7621 gttcttattt tttcgtcttt ccttgtctat ctggtggaaa aggatgccaa taaagagttt
     7681 tctacatatg cagatgctct ctggtggggc acaattacat tgacaactat tggctatgga
     7741 gacaaaactc ccctaacttg gctgggaaga ttgctttctg caggctttgc actccttggc
     7801 atttctttct ttgcacttcc tgccggcatt cttggctcag gttttgcatt aaaagtacaa
     7861 gaacaacacc gccagaaaca ctttgagaaa agaaggaacc cagctgccaa cctcattcag
     7921 tgtgtttggc gtagttacgc agctgatgag aaatctgttt ccattgcaac ctggaagcca
     7981 cacttgaagg ccttgcacac ctgcagccct accaagaaag aacaagggga agcatcaagc
     8041 agttggggac agtggacatt gcgtcagaag ctaagtttta aggagcgagt gcgcatggct
     8101 agccccaggg gccagagtat taagagccga caagcctcag taggtgacag gaggtcccca
     8161 agcaccgaca tcacagccga gggcagtccc accaaagtgc agaagagctg gagcttcaac
     8221 gaccgaaccc gcttccggcc ctcgctgcgc ctcaaaagtt ctcagccaaa accagtgata
     8281 gatgctgaca cagcccttgg cactgatgat gtatatgatg aaaaaggatg ccagtgtgat
     8341 gtatcagtgg aagacctcac cccaccactt aaaactgtca ttcgagctat cagaattatg
     8401 aaatttcatg ttgcaaaacg gaagtttaag gaaacattac gtccatatga tgtaaaagat
     8461 gtcattgaac aatattctgc tggtcatctg gacatgttgt gtagaattaa aagccttcaa
     8521 acacgtgttg atcaaattct tggaaaaggg caaatcacat cagataagaa gagccgagag
     8581 aaaataacag cagaacatga gaccacagac gatctcagta tgctcggtcg ggtggtcaag
     8641 gttgaaaaac aggtacagtc catagaatcc aagctggact gcctactaga catctatcaa
     8701 caggtccttc ggaaaggctc tgcctcagcc ctcgctttgg cttcattcca gatcccacct
     8761 tttgaatgtg aacagacatc tgactatcaa agccctgtgg atagcaaaga tctttcgggt
     8821 tccgcacaaa acagtggctg cttatccaga tcaactagtg ccaacatctc gagaggcctg
     8881 cagttcattc tgacgccaaa tgagttcagt gcccagactt tctacgcgct tagccctact
     8941 atgcacagtc aagcaacaca ggtgccaatt agtcaaagcg atggctcagc agtggcagcc
     9001 accaacacca ttgcaaacca aataaatacg gcacccaagc cagcagcccc aacaacttta
     9061 cagatcccac ctcctctccc agccatcaag catctgccca ggccagaaac tctgcaccct
     9121 aaccctgcag gcttacagga aagcatttct gacgtcacca cctgccttgt tgcctccaag
     9181 gaaaatgttc aggttgcaca gtcaaatctc accaaggacc gttctatgag gaaaagcttt
     9241 gacatgggag gagaaactct gttgtctgtc tgtcccatgg tgccgaagga cttgggcaaa
     9301 tctttgtctg tgcaaaacct gatcaggtcg accgaggaac tgaatataca actttcaggg
     9361 agtgagtcaa gtggctccag aggcagccaa gatttttacc ccaaatggag ggaatccaaa
     9421 ttgtttataa ctgatgaaga ggtgggtccc gaagagacag agacagacac ttttgatgcc
     9481 gcaccgcagc ctgccaggga agctgccttt gcatcagact ctctaaggac tggaaggtca
     9541 cgatcatctc agagcatttg taaggcagga gaaagtacag atgccctcag cttgcctcat
     9601 gtcaaactga aataagttct tcattttctt tccaggcata gcagttcttt agccatacat
     9661 atcattgcat gaactatttc gaaagccctt ctaaaaagtt gaaattgcaa gaatcgggaa
     9721 gaacatgaaa ggcagtttat aagcccgtta ccttttaatt gcatgaaaat gcatgtttag
     9781 ggatggctaa aattccaagg tgcatcgaca ttaacccact catttagtaa tgtaccttga
     9841 gttaaaaagc ctgagaaacc aaacacagct aatgctatgg ggtgtatgaa tatgtcaagt
     9901 ttaggtcatt tagaagattt gacactgtat tttgaaatta tgggagtaaa caccttcaaa
     9961 tttcaggcat ttctgctttg tgactaaata caaactacat tttcaagatt aggccataat
    10021 gtatatttaa acacaatggc tatcaacagc tgctaataag gtatcaacta aagcagaatt
    10081 ggggaataat agaaatggct gcttatttca agatatattt gccaacccat tcctattcag
    10141 tcattttatt attaatgtaa tttgaatgtc aatttgtgtg cttttggtga tttagcgccc
    10201 gggatccgcc cctctccctc cccc
//
