LOCUS       sequence-404008-        9528 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Heterologous expression in mammalian cell lines or Xenopus oocyte 
            (T7 RNA pol).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9528)
  AUTHORS   Wei AD, Wakenight P, Zwingman TA, Bard AM, Sahai N, Willemsen MH, 
            Schelhaas HJ, Stegmann APA, Verhoeven JS, de Man SA, Wessels MW, 
            Kleefstra T, Shinde DN, Helbig KL, Basinger A, Wagner VF, 
            Rodriguez-Buritica D, Bryant E, Millichap JJ, Millen KJ, Dobyns WB, 
            Ramirez JM, Kalume FK
  TITLE     Human KCNQ5 de novo mutations underlie epilepsy and intellectual 
            disability.
  JOURNAL   J Neurophysiol. 2022 Jul 1;128(1):40-61. doi: 10.1152/jn.00509.2021.
            Epub 2022 May 18.
  PUBMED    35583973
REFERENCE   2  (bases 1 to 9528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1152/jn.00509.2021"; journalName: "J Neurophysiol"; date: 
            "2022-07-1- 1"; volume: "128"; issue: "1"; pages: "40-61"
COMMENT     Sequence Label: hKCNQ3(WT).ires.mCherry-pcDNA3
FEATURES             Location/Qualifiers
     source          1..9528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1211..1230)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1402..1781
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1782..1985
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1936..1956
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2030..2049
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2030..2048
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2105..2114
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(4758..4782)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4797..5370
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4820..5372
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4964..4981)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5191..5210
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5372..6082
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5517..5535)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5785..5804
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(6117..6134)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6123..6347
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6393..6821
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6480..6499)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6690..6711
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6830..6850)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6835..7164
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7015..7150
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7077..7096
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7213..8238
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7213..8238
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    8368..8489
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8405..8424)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8459..8478
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8538..8554)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8538..8554)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8551..8573)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8562..8578
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8586..8592,8593..8610,8611..8616))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8631..8652
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8769..8786)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8940..9528)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9020..9039)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     1201 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     1261 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     1321 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
     1381 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
     1441 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     1501 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     1561 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     1621 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     1681 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     1741 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     1801 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     1861 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     1921 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     1981 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
     2041 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
     2101 cgccgccacc atggggctca aggcgcgcag ggcggcgggg gcggctggcg gcggcggcga
     2161 cgggggcggc ggaggcggcg gggcggctaa cccagccgga ggggacgcgg cggcggccgg
     2221 cgacgaggag cggaaagtgg ggctggcgcc cggcgacgtg gagcaagtca ccttggcgct
     2281 cggggccgga gccgacaaag acgggaccct gctgctggag ggcggcggcc gcgacgaggg
     2341 gcagcggagg accccgcagg gcatcgggct cctggccaag accccgctga gccgcccagt
     2401 caagagaaac aacgccaagt accggcgcat ccaaactttg atctacgacg ccctggagag
     2461 accgcggggc tgggcgctgc tttaccacgc gttggtgttc ctgattgtcc tggggtgctt
     2521 gattctggct gtcctgacca cattcaagga gtatgagact gtctcgggag actggcttct
     2581 gttactggag acatttgcta ttttcatctt tggagccgag tttgctttga ggatctgggc
     2641 tgctggatgt tgctgccgat acaaaggctg gcggggccga ctgaagtttg ccaggaagcc
     2701 cctgtgcatg ttggacatct ttgtgctgat tgcctctgtg ccagtggttg ctgtgggaaa
     2761 ccaaggcaat gttctggcca cctccctgcg aagcctgcgc ttcctgcaga tcctgcgcat
     2821 gctgcggatg gaccggagag gtggcacctg gaagcttctg ggctcagcca tctgtgccca
     2881 cagcaaagaa ctcatcacgg cctggtacat cggtttcctg acactcatcc tttcttcatt
     2941 tcttgtctac ctggttgaga aagacgtccc agaggtggat gcacaaggag aggagatgaa
     3001 agaggagttt gagacctatg cagatgccct gtggtggggc ctgatcacac tggccaccat
     3061 tggctatgga gacaagacac ccaaaacgtg ggaaggccgt ctgattgccg ccaccttttc
     3121 cttaattggc gtctcctttt ttgcccttcc agcgggcatc ctggggtccg ggctggccct
     3181 caaggtgcag gagcaacacc gtcagaagca ctttgagaaa aggaggaagc cagctgctga
     3241 gctcattcag gctgcctgga ggtattatgc taccaacccc aacaggattg acctggtggc
     3301 gacatggaga ttttatgaat cagtcgtctc ttttcctttc ttcaggaaag aacagctgga
     3361 ggcagcatcc agccaaaagc tgggtctctt ggatcgggtt cgcctttcta atcctcgtgg
     3421 tagcaatact aaaggaaagc tatttacccc tctgaatgta gatgccatag aagaaagtcc
     3481 ttctaaagaa ccaaagcctg ttggcttaaa caataaagag cgtttccgca cggccttccg
     3541 catgaaagcc tacgctttct ggcagagttc tgaagatgcc gggacaggtg accccatggc
     3601 ggaagacagg ggctatggga atgacttccc catcgaagac atgatcccca ccctgaaggc
     3661 cgccatccga gccgtcagaa ttctacaatt ccgtctctat aaaaaaaaat tcaaggagac
     3721 tttgaggcct tacgatgtga aggatgtgat tgagcagtat tctgccgggc atctcgacat
     3781 gctttccagg ataaagtacc ttcagacgag aatagatatg attttcaccc ctggacctcc
     3841 ctccacgcca aaacacaaga agtctcagaa agggtcagca ttcaccttcc catcccagca
     3901 atctcccagg aatgaaccat atgtagccag accatccaca tcagaaatcg aagaccaaag
     3961 catgatgggg aagtttgtaa aagttgaaag acaggttcag gacatgggga agaagctgga
     4021 cttcctcgtg gatatgcaca tgcaacacat ggaacggttg caggtgcagg tcacggagta
     4081 ttacccaacc aagggcacct cctcgccagc tgaagcagag aagaaggagg acaacaggta
     4141 ttccgatttg aaaaccatca tctgcaacta ttctgagaca ggccccccgg aaccacccta
     4201 cagcttccac caggtgacca ttgacaaagt cagcccctat gggttttttg cacatgaccc
     4261 tgtgaacctg ccccgagggg gacccagttc tggaaaggtt caggcaactc ctccttcctc
     4321 agcaacaacg tatgtggaga ggcccacggt cctgcctatc ttgactcttc tcgactcccg
     4381 agtgagctgc cactcccagg ctgacctgca gggcccctac tcggaccgaa tctccccccg
     4441 gcagagacgt agcatcacgc gagacagtga cacacctctg tccctgatgt cggtcaacca
     4501 cgaggagctg gagaggtctc caagtggctt cagcatctcc caggacagag atgattatgt
     4561 gttcggcccc aatggggggt cgagctggat gagggagaag cggtacctcg ccgagggtga
     4621 gacggacaca gacacggacc ccttcacgcc cagcggctcc atgcctctgt cgtccacagg
     4681 ggatgggatt tctgattcag tatggacccc ttccaataag cccatttaac tcgagatcga
     4741 ttgttaacgg cgcgccgacc cagctttctt gtacaaagtg gttgatggca attccgcccc
     4801 tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg
     4861 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
     4921 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
     4981 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
     5041 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcaacag gtgcctctgc
     5101 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
     5161 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg
     5221 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca
     5281 tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac
     5341 gtggttttcc tttgaaaaac acgatgataa tatggtgagc aagggcgagg aggataacat
     5401 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
     5461 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     5521 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
     5581 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
     5641 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     5701 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
     5761 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     5821 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     5881 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     5941 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     6001 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     6061 catggacgag ctgtacaagt aaccgtctag agggcccgtt taaacccgct gatcagcctc
     6121 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     6181 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     6241 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     6301 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     6361 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     6421 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     6481 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     6541 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     6601 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     6661 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     6721 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     6781 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     6841 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     6901 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     6961 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     7021 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     7081 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     7141 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     7201 gatcagcacg tgatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc
     7261 gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct
     7321 ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt
     7381 ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa
     7441 gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag
     7501 ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg
     7561 gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc
     7621 ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat
     7681 ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag
     7741 gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac
     7801 gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac
     7861 tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg
     7921 ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt
     7981 gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag
     8041 agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc
     8101 gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc
     8161 tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt
     8221 ccgagggcaa aggaatagca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa
     8281 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     8341 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     8401 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     8461 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     8521 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     8581 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     8641 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     8701 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     8761 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     8821 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     8881 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     8941 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     9001 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     9061 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     9121 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     9181 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     9241 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     9301 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     9361 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     9421 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     9481 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaa
//
