LOCUS       sequence-404009-        8469 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Heterologous expression in mammalian cell lines or Xenopus oocyte 
            (T7 RNA pol).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8469)
  AUTHORS   Wei AD, Wakenight P, Zwingman TA, Bard AM, Sahai N, Willemsen MH, 
            Schelhaas HJ, Stegmann APA, Verhoeven JS, de Man SA, Wessels MW, 
            Kleefstra T, Shinde DN, Helbig KL, Basinger A, Wagner VF, 
            Rodriguez-Buritica D, Bryant E, Millichap JJ, Millen KJ, Dobyns WB, 
            Ramirez JM, Kalume FK
  TITLE     Human KCNQ5 de novo mutations underlie epilepsy and intellectual 
            disability.
  JOURNAL   J Neurophysiol. 2022 Jul 1;128(1):40-61. doi: 10.1152/jn.00509.2021.
            Epub 2022 May 18.
  PUBMED    35583973
REFERENCE   2  (bases 1 to 8469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1152/jn.00509.2021"; journalName: "J Neurophysiol"; date: 
            "2022-07-1- 1"; volume: "128"; issue: "1"; pages: "40-61"
COMMENT     Sequence Label: hBACE1(D289N).ires.mCherry-pcDNA3
FEATURES             Location/Qualifiers
     source          1..8469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     63..80
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     protein_bind    262..286
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1829..1853)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(1936..1955)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1937..1955)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2000..2203)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2029..2049)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2204..2583
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     2755..2774
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        2849..2953
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2954..3022,3023..3814)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3172..3191)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3985..4573
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4474..4493
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4727..4744
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4861..4882
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4897..4902,4903..4920,4921..4927)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4935..4951
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4940..4962
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4959..4975
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4959..4975
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    5024..5145
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5035..5054)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5089..5108
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(5275..6300)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(5275..6300)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(6349..6678)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6363..6498
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6417..6436)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     6663..6683
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(6692..7120)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6802..6823)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7014..7033
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    7166..7390
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     7379..7396
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(7431..8141)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7709..7728)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     7978..7996
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     misc_feature    join(8141..8469,1..224)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    join(8143..8469,1..247)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8303..8322)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 cgttgtttgt cttcaagaag cttccagagg aactgcttcc ttcacgacat tcaacagacc
       61 ttgcattcct ttggcgagag gggaaagacc cctaggaatg ctcgtcaaga agacagggcc
      121 aggtttccgg gccctcacat tgccaaaaga cggcaatatg gtggaaaata acatatagac
      181 aaacgcacac cggccttatt ccaagcggct tcggccagta acgttagggg ggggggaggg
      241 agaggggcgg aattgccatc aaccactttg tacaagaaag ctgggtcggc gcgcccaccc
      301 tttcacttca gcagggagat gtcatcagca aagtcatcat gctgctggcg caggcagcgg
      361 aggcagcgcc actgacacac catgaggcag agtggcagca tgaagagggc gcagatggca
      421 gccatgacat aggctatggt catgagggtt gactcatctg tctgtggaat gttgtagcca
      481 cagtcttcca tgtccaaggt gacaaaaggg ccttccaccg ctgccgtcct gaactcatcg
      541 tgcacatggc aagcgctgac agcaaagcca attcgttttc gggcccgatc aaagacaacg
      601 tagaagccct ccatgataac agctcccata acagtgcccg tggatgactg tgagatggca
      661 aacttgtaac agtcgtcttg ggacgtggcc acatcttcca ctggccgcag gtattgctgc
      721 ggaaggatgg tgatgcggaa ggactggttg gtaacctcac ccattaggta gagtgagatg
      781 actgggaaaa tgttccaagg ggtggtgcct gcttgccagc acaccagctg ctctcctagc
      841 cagaaaccat cagggaactt ctccgtggag gaggctgcct tgatggattt gactgcagct
      901 tcaaacactt tcttgggcaa acgaaggttg gtggtgccac tgttcacaat gctcttgtca
      961 tagttgtact ccttgcagtc cattttcaga tcctgtccat tgatctccac ccgcacaatg
     1021 atcacctcat aataccactc ccgccggatg ggtgtatacc agagactgcc tgtgtacagc
     1081 gagtggtcga tacctccaat gatcatgctc cctccgacag aggccagcac ttcagactgg
     1141 ttgaggggga agccagcacc acaaagctgc agggagaaga ggttgggaac gtgggtctgc
     1201 tttaccagag agtcaaagaa aggctccagg gagtcgtcag gcctggcaat ctcagcatag
     1261 gccagcccca ggatgccttc ccagttggag ccgttgatga agaacttgtc tgattcagtg
     1321 atggcagcaa tgttggcacg cacagtgacg ttggggccat gggggatgct taccaggtcg
     1381 gtgcccagct ccccttccca cttgccctgg gtgtagggca catacacacc cttccggagg
     1441 tcccggtatg tgctggacag ctgcctctgg tagtagcgat gcaggaaggg gtggggggca
     1501 gcacccactg caaagttact gctgcctgta tccaccagga tgttgagcgt ctgcgggggg
     1561 ctgcccacgg tcatctccac gtagtagccc tgccccgact tgcccctcag gttgtccacc
     1621 atctccacaa agctgcccct ccggccgggc tcctcgggct cttcgtcggt ctcccggggc
     1681 agccgcagcc ccaggggggc gccccccagg ccgctgcgca ggggcagccg gatgccgtgc
     1741 tgggtgccgt gggcaggcag cactcccgcg cccatccaca gcaggagcca gggcagggct
     1801 tgggccatgg tgaagggggc ggccgcggag cctgcttttt tgtacaaact tgtatctgca
     1861 gaattccacc acactggact agtggatccg agctcggtac caagcttaag tttaaacgct
     1921 agccagcttg ggtctcccta tagtgagtcg tattaatttc gataagccag taagcagtgg
     1981 gttctctagt tagccagaga gctctgctta tatagacctc ccaccgtaca cgcctaccgc
     2041 ccatttgcgt caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt
     2101 gccaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa
     2161 accgctatcc acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga
     2221 ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat
     2281 gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca
     2341 cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga
     2401 aagtccctat tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt
     2461 cgttgggcgg tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat
     2521 atgggctatg aactaatgac cccgtaattg attactatta ataactagtc aataatcaat
     2581 gtcaacgcgt atatctggcc cgtacatcgc gaagcagcgc aaaacgccta accctaagca
     2641 gattcttcat gcaattgtcg gtcaagcctt gccttgttgt agcttaaatt ttgctcgcgc
     2701 actactcagc gacctccaac acacaagcag ggagcagata ctggcttaac tatgcggcat
     2761 cagagcagat tgtactgaga gtgcaccata ggggatcggg agatctcccg atccgtcgac
     2821 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     2881 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     2941 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     3001 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     3061 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     3121 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     3181 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     3241 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     3301 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     3361 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     3421 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     3481 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     3541 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     3601 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     3661 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     3721 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     3781 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     3841 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     3901 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     3961 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     4021 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     4081 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     4141 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     4201 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     4261 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     4321 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     4381 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     4441 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     4501 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     4561 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     4621 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     4681 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     4741 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     4801 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     4861 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     4921 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     4981 ttacgccaag ctctagctag aggtcgacgg tatacagaca tgataagata cattgatgag
     5041 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     5101 gctattgctt tatttgtaac cattataagc tgcaataaac aagttggggt gggcgaagaa
     5161 ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc
     5221 gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtagca cgtgctattc
     5281 ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta cttctacaca
     5341 gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc
     5401 tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc
     5461 gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg cccggagccg
     5521 cggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct gctccataca
     5581 agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat ccccgaacat
     5641 cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga cattgttgga
     5701 gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc aaagcatcag
     5761 ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag tttgccagtg
     5821 atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt attgaccgat
     5881 tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg cagcgatcgc
     5941 atccatggcc tccgcgaccg gctgcagaac agcgggcagt tcggtttcag gcaggtcttg
     6001 caacgtgaca ccctgtgcac ggcgggagat gcaataggtc aggctctcgc tgaattcccc
     6061 aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat aaacataacg
     6121 atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac gccctcctac
     6181 atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt cggagacgct
     6241 gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag gctttttcat
     6301 cacgtgctga tcagatccga aaatggatat acaagctccc gggagctttt tgcaaaagcc
     6361 taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggcag aggcggcctc
     6421 ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg
     6481 cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag
     6541 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc
     6601 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
     6661 acaccctaac tgacacacat tccacagaat taattcgcgt taaatttttg ttaaatcagc
     6721 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     6781 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     6841 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     6901 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     6961 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     7021 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     7081 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ggggataccc cctagagccc
     7141 cagctggttc tttccgcctc agaagccata gagcccaccg catccccagc atgcctgcta
     7201 ttgtcttccc aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga
     7261 cacctactca gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg
     7321 gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta
     7381 gaaggcacag tcgaggctga tcagcgggtt taaacgggcc ctctagacgg ttacttgtac
     7441 agctcgtcca tgccgccggt ggagtggcgg ccctcggcgc gttcgtactg ttccacgatg
     7501 gtgtagtcct cgttgtggga ggtgatgtcc aacttgatgt tgacgttgta ggcgccgggc
     7561 agctgcacgg gcttcttggc cttgtaggtg gtcttgacct cagcgtcgta gtggccgccg
     7621 tccttcagct tcagcctctg cttgatctcg cccttcaggg cgccgtcctc ggggtacatc
     7681 cgctcggagg aggcctccca gcccatggtc ttcttctgca ttacggggcc gtcggagggg
     7741 aagttggtgc cgcgcagctt caccttgtag atgaactcgc cgtcctgcag ggaggagtcc
     7801 tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca cgcgctccca cttgaagccc
     7861 tcggggaagg acagcttcaa gtagtcgggg atgtcggcgg ggtgcttcac gtaggccttg
     7921 gagccgtaca tgaactgagg ggacaggatg tcccaggcga agggcagggg gccacccttg
     7981 gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc ctcgccctcg
     8041 atctcgaact cgtggccgtt cacggagccc tccatgtgca ccttgaagcg catgaactcc
     8101 ttgatgatgg ccatgttatc ctcctcgccc ttgctcacca tattatcatc gtgtttttca
     8161 aaggaaaacc acgtccccgt ggttcggggg gcctagacgt ttttttaacc tcgactaaac
     8221 acatgtaaag catgtgcacc gaggccccag atcagatccc atacaatggg gtaccttctg
     8281 ggcatccttc agccccttgt tgaatacgct tgaggagagc catttgactc tttccacaac
     8341 tatccaactc acaacgtggc actggggttg tgccgccttt gcaggtgtat cttatacacg
     8401 tggcttttgg ccgcagaggc acctgttgcc aggtgggggg ttccgctgcc tgcaaagggt
     8461 cgctacaga
//
