LOCUS       sequence-404012-       10235 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Heterologous expression in mammalian cell lines or Xenopus oocyte 
            (T7 RNA pol).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10235)
  AUTHORS   Wei AD, Wakenight P, Zwingman TA, Bard AM, Sahai N, Willemsen MH, 
            Schelhaas HJ, Stegmann APA, Verhoeven JS, de Man SA, Wessels MW, 
            Kleefstra T, Shinde DN, Helbig KL, Basinger A, Wagner VF, 
            Rodriguez-Buritica D, Bryant E, Millichap JJ, Millen KJ, Dobyns WB, 
            Ramirez JM, Kalume FK
  TITLE     Human KCNQ5 de novo mutations underlie epilepsy and intellectual 
            disability.
  JOURNAL   J Neurophysiol. 2022 Jul 1;128(1):40-61. doi: 10.1152/jn.00509.2021.
            Epub 2022 May 18.
  PUBMED    35583973
REFERENCE   2  (bases 1 to 10235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1152/jn.00509.2021"; journalName: "J Neurophysiol"; date: 
            "2022-07-1- 1"; volume: "128"; issue: "1"; pages: "40-61"
COMMENT     Sequence Label: hKCNQ5(P369T).ires.GFP-pcDNA3
FEATURES             Location/Qualifiers
     source          1..10235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(36..55)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     282..302
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(311..739)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(421..442)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     633..652
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    785..1009
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     998..1015
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        1035..1053
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1035..1052
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(join(1069..1782,1783..1785,1786..1788))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(1072..1782,1783..1785,1786..1788))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     1463..1482
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1722..1743
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    join(1789..1797,1798..1800,1801..2375)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1962..1981)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     2191..2208
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     5'UTR           5879..5921
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     primer_bind     complement(5959..5978)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5960..5978)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(6023..6226)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6052..6072)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6227..6606
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     6778..6797
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6872..6976
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6977..7045,7046..7837)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7195..7214)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8008..8596
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8497..8516
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8750..8767
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8884..8905
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8920..8925,8926..8943,8944..8950)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8958..8974
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8963..8985
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8982..8998
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8982..8998
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    9047..9168
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9058..9077)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9112..9131
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(9342..10136)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9453..9472)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     10063..10082
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(10203..10235,1..297))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(10217..10235,1..117)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 tcactacttc tggaatagct cagaggcaga ggcggcctcg gcctctgcat aaataaaaaa
       61 aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg
      121 cggagttagg ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct
      181 gcctgctggg gagcctgggg actttccaca cctggttgct gactaattga gatgcatgct
      241 ttgcatactt ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt
      301 ccacagaatt aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
      361 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
      421 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
      481 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
      541 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
      601 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
      661 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
      721 gcgccgctac agggcgcgtg gggatacccc ctagagcccc agctggttct ttccgcctca
      781 gaagccatag agcccaccgc atccccagca tgcctgctat tgtcttccca atcctccccc
      841 ttgctgtcct gccccacccc accccccaga atagaatgac acctactcag acaatgcgat
      901 gcaatttcct cattttatta ggaaaggaca gtgggagtgg caccttccag ggtcaaggaa
      961 ggcacggggg aggggcaaac aacagatggc tggcaactag aaggcacagt cgaggctgat
     1021 cagcgagctc tagcatttag gtgacactat agaatagggc cctctagatt acttgtacag
     1081 ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc
     1141 gcgcttctcg ttggggtctt tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg
     1201 cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac
     1261 gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt tcttctgctt
     1321 gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat
     1381 gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc
     1441 ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg
     1501 ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc
     1561 ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg
     1621 cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc
     1681 gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac
     1741 caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgg ttgtggccat
     1801 attatcatcg tgtttttcaa aggaaaacca cgtccccgtg gttcgggggg cctagacgtt
     1861 tttttaacct cgactaaaca catgtaaagc atgtgtaccg aggccccaga tcagatccca
     1921 tacaatgggg taccttctgg gcatccttca gccccttgtt gaatacgctt gaggagagcc
     1981 atttgactct ttccacaact atccaactca caacgtggca ctggggttgt gccgcctttg
     2041 caggtgtatc ttatacacgt ggcttttggc cgcagaggca cctgtcgcca ggtggggggt
     2101 tccgctgcct gcaaagggtc gctacagacg ttgtttgtct tcaagaagct tccagaggaa
     2161 ctgcttcctt cacgacattc aacagacctt gcattccttt ggcgagaggg gaaagacccc
     2221 taggaatgct cgtcaagaag acagggccag gtttccgggc cctcacattg ccaaaagacg
     2281 gcaatatggt ggaaaataac atatagacaa acgcacaccg gccttattcc aagcggcttc
     2341 ggccagtaac gttagggggg ggggagggag aggggcggat cccgggcgct aaatcaccaa
     2401 aagcacacaa attgacattc aaattacatt aataataaaa tgactgaata ggaatgggtt
     2461 ggcaaatata tcttgaaata agcagccatt tctattattc cccaattctg ctttagttga
     2521 taccttatta gcagctgttg atagccattg tgtttaaata tacattatgg cctaatcttg
     2581 aaaatgtagt ttgtatttag tcacaaagca gaaatgcctg aaatttgaag gtgtttactc
     2641 ccataatttc aaaatacagt gtcaaatctt ctaaatgacc taaacttgac atattcatac
     2701 accccatagc attagctgtg tttggtttct caggcttttt aactcaaggt acattactaa
     2761 atgagtgggt taatgtcgat gcaccttgga attttagcca tccctaaaca tgcattttca
     2821 tgcaattaaa aggtaacggg cttataaact gcctttcatg ttcttcccga ttcttgcaat
     2881 ttcaactttt tagaagggct ttcgaaatag ttcatgcaat gatatgtatg gctaaagaac
     2941 tgctatgcct ggaaagaaaa tgaagaactt atttcagttt gacatgaggc aagctgaggg
     3001 catctgtact ttctcctgcc ttacaaatgc tctgagatga tcgtgacctt ccagtcctta
     3061 gagagtctga tgcaaaggca gcttccctgg caggctgcgg tgcggcatca aaagtgtctg
     3121 tctctgtctc ttcgggaccc acctcttcat cagttataaa caatttggat tccctccatt
     3181 tggggtaaaa atcttggctg cctctggagc cacttgactc actccctgaa agttgtatat
     3241 tcagttcctc ggtcgacctg atcaggtttt gcacagacaa agatttgccc aagtccttcg
     3301 gcaccatggg acagacagac aacagagttt ctcctcccat gtcaaagctt ttcctcatag
     3361 aacggtcctt ggtgagattt gactgtgcaa cctgaacatt ttccttggag gcaacaaggc
     3421 aggtggtgac gtcagaaatg ctttcctgta agcctgcagg gttagggtgc agagtttctg
     3481 gcctgggcag atgcttgatg gctgggagag gaggtgggat ctgtaaagtt gttggggctg
     3541 ctggcttggg tgccgtattt atttggtttg caatggtgtt ggtggctgcc actgctgagc
     3601 catcgctttg actaattggc acctgtgttg cttgactgtg catagtaggg ctaagcgcgt
     3661 agaaagtctg ggcactgaac tcatttggcg tcagaatgaa ctgcaggcct ctcgagatgt
     3721 tggcactagt tgatctggat aagcagccac tgttttgtgc ggaacccgaa agatctttgc
     3781 tatccacagg gctttgatag tcagatgtct gttcacattc aaaaggtggg atctggaatg
     3841 aagccaaagc gagggctgag gcagagcctt tccgaaggac ctgttgatag atgtctagta
     3901 ggcagtccag cttggattct atggactgta cctgtttttc aaccttgacc acccgaccga
     3961 gcatactgag atcgtctgtg gtctcatgtt ctgctgttat tttctctcgg ctcttcttat
     4021 ctgatgtgat ttgccctttt ccaagaattt gatcaacacg tgtttgaagg cttttaattc
     4081 tacacaacat gtccagatga ccagcagaat attgttcaat gacatctttt acatcatatg
     4141 gacgtaatgt ttccttaaac ttccgttttg caacatgaaa tttcataatt ctgatagctc
     4201 gaatgacagt tttaagtggt ggggtgaggt cttccactga tacatcacac tggcatcctt
     4261 tttcatcata tacatcatca gtgccaaggg ctgtgtcagc atctatcact ggttttggct
     4321 gagaactttt gaggcgcagc gagggccgga agcgggttcg gtcgttgaag ctccagctct
     4381 tctgcacttt ggtgggactg ccctcggctg tgatgtcggt gcttggggac ctcctgtcac
     4441 ctactgaggc ttgtcggctc ttaatactct ggcccctggg gctagccatg cgcactcgct
     4501 ccttaaaact tagcttctga cgcaatgtcc actgtcccca actgcttgat gcttcccctt
     4561 gttctttctt ggtagggctg caggtgtgca aggccttcaa gtgtggcttc caggttgcaa
     4621 tggaaacaga tttctcatca gctgcgtaac tacgccaaac acactgaatg aggttggcag
     4681 ctgtgttcct tcttttctca aagtgtttct ggcggtgttg ttcttgtact tttaatgcaa
     4741 aacctgagcc aagaatgccg gcaggaagtg caaagaaaga aatgccaagg agtgcaaagc
     4801 ctgcagaaag caatcttccc agccaagtta ggggagtttt gtctccatag ccaatagttg
     4861 tcaatgtaat tgtgccccac cagagagcat ctgcatatgt agaaaactct ttattggcat
     4921 ccttttccac cagatagaca aggaaagacg aaaaaataag aaccaaaaat cctatgtacc
     4981 aagctgtgat taattccttg ctgtgagcat aaaccactga acccagtaat ttccaagtgc
     5041 ctccccttcg gtccatgcgc accatgcgga ggatctgtag gaaacggaga cttctgagtg
     5101 cagacgtggc aaaaatatta ccctgagttt ttgcagaaac aactgctatt gaagcgataa
     5161 gaacaatggt atctataaca cagaagggct ttcgagcaaa cctcagtctt ccttgccatc
     5221 ctctatatcg acaacagcaa cccgcagacc agattcgaat gatgaactcc aaaccaaaga
     5281 cgacaatcat cacgaactcc aggatcaaga ggcaacttga ggccaatttt gtgtgctcag
     5341 ggatggtaga aaacactgac aaaatcaagc aaccaaagac aaggagaaaa acgaaagcgt
     5401 ggtagatgaa cgcccagccg cggggtctct ccagcacgtt gtacaggtag ttctgcaccc
     5461 gccggtactt gacgttgcgc cggcagctct ggctactcgt gtaagagagc ggcttcccca
     5521 gcaggctcat ccgggccccc tgcttgcccc ggcggctctc cctcaggcca ccgccgccgc
     5581 caccaagcgt ggccgcgcgg gtgcccagca gtagcaggcc gtcgcccctg gcggctgccg
     5641 agttcagcag caccctgccc cggcccgact ccacatcctt catgccgctg cccaagcgcc
     5701 ccccgcccgc cgccgccgcc gctgcgccgc tcttcaccca gagcccggcg gcgccgccct
     5761 cctctcctcc cgcgtggtgg cggggcatgg catctagagc ggccgccagt gtgatggata
     5821 tctgcagaat tccagcacac tggcggccgt tactagtgga tccgagctcg gtacccttct
     5881 tgttcttttt gcagaagctc agaataaacg ctcaactttg gcagagctag cgtcgacggt
     5941 atcgataagc ttgggtctcc ctatagtgag tcgtattaat ttcgataagc cagtaagcag
     6001 tgggttctct agttagccag agagctctgc ttatatagac ctcccaccgt acacgcctac
     6061 cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt
     6121 ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt
     6181 caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga
     6241 tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat
     6301 aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat
     6361 acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat
     6421 ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg
     6481 ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca
     6541 tatatgggct atgaactaat gaccccgtaa ttgattacta ttaataacta gtcaataatc
     6601 aatgtcaacg cgtatatctg gcccgtacat cgcgaagcag cgcaaaacgc ctaaccctaa
     6661 gcagattctt catgcaattg tcggtcaagc cttgccttgt tgtagcttaa attttgctcg
     6721 cgcactactc agcgacctcc aacacacaag cagggagcag atactggctt aactatgcgg
     6781 catcagagca gattgtactg agagtgcacc ataggggatc gggagatctc ccgatccgtc
     6841 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     6901 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     6961 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     7021 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     7081 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     7141 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     7201 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     7261 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     7321 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     7381 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     7441 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     7501 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     7561 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     7621 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     7681 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     7741 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     7801 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     7861 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     7921 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     7981 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     8041 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     8101 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     8161 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     8221 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     8281 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     8341 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     8401 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     8461 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     8521 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     8581 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     8641 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     8701 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     8761 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     8821 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     8881 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     8941 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     9001 tgattacgcc aagctctagc tagaggtcga cggtatacag acatgataag atacattgat
     9061 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     9121 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttgg ggtgggcgaa
     9181 gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc ggaaaacgat
     9241 tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt
     9301 gggcgtcgct tggtcggtca tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga
     9361 aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag
     9421 cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc
     9481 tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt
     9541 tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg
     9601 ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg
     9661 tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga
     9721 tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt
     9781 gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc
     9841 cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca
     9901 gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt
     9961 tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac
    10021 agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat
    10081 agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatgcga
    10141 aacgatcctc atcctgtctc ttgatcagat ccgaaaatgg atatacaagc tcccgggagc
    10201 tttttgcaaa agcctaggcc tccaaaaaag cctcc
//
