LOCUS       sequence-404014-       10235 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Heterologous expression in mammalian cell lines or Xenopus oocyte 
            (T7 RNA pol).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10235)
  AUTHORS   Wei AD, Wakenight P, Zwingman TA, Bard AM, Sahai N, Willemsen MH, 
            Schelhaas HJ, Stegmann APA, Verhoeven JS, de Man SA, Wessels MW, 
            Kleefstra T, Shinde DN, Helbig KL, Basinger A, Wagner VF, 
            Rodriguez-Buritica D, Bryant E, Millichap JJ, Millen KJ, Dobyns WB, 
            Ramirez JM, Kalume FK
  TITLE     Human KCNQ5 de novo mutations underlie epilepsy and intellectual 
            disability.
  JOURNAL   J Neurophysiol. 2022 Jul 1;128(1):40-61. doi: 10.1152/jn.00509.2021.
            Epub 2022 May 18.
  PUBMED    35583973
REFERENCE   2  (bases 1 to 10235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1152/jn.00509.2021"; journalName: "J Neurophysiol"; date: 
            "2022-07-1- 1"; volume: "128"; issue: "1"; pages: "40-61"
COMMENT     Sequence Label: hKCNQ5(R443Q).ires.GFP-pcDNA3
FEATURES             Location/Qualifiers
     source          1..10235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1211..1230)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1402..1781
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1782..1985
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1936..1956
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2030..2049
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2030..2048
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           complement(2087..2129)
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     misc_feature    join(5633..6207,6208..6210,6211..6219)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5800..5817)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6027..6046
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6220..6222,6223..6225,6226..6939)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6220..6222,6223..6225,6226..6936)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6265..6286)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6526..6545)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     promoter        complement(6955..6973)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6956..6973)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6993..7010)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6999..7223
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7269..7697
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7356..7375)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7566..7587
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7706..7726)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7711..8040
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7891..8026
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7953..7972
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             8107..8901
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8161..8180)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8771..8790
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9075..9196
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9112..9131)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9166..9185
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9245..9261)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9245..9261)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9258..9280)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9269..9285
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9293..9299,9300..9317,9318..9323))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9338..9359
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9476..9493)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9647..10235)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9727..9746)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     1201 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     1261 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     1321 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
     1381 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
     1441 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     1501 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     1561 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     1621 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     1681 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     1741 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     1801 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     1861 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     1921 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     1981 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
     2041 actataggga gacccaagct tatcgatacc gtcgacgcta gctctgccaa agttgagcgt
     2101 ttattctgag cttctgcaaa aagaacaaga agggtaccga gctcggatcc actagtaacg
     2161 gccgccagtg tgctggaatt ctgcagatat ccatcacact ggcggccgct ctagatgcca
     2221 tgccccgcca ccacgcggga ggagaggagg gcggcgccgc cgggctctgg gtgaagagcg
     2281 gcgcagcggc ggcggcggcg ggcggggggc gcttgggcag cggcatgaag gatgtggagt
     2341 cgggccgggg cagggtgctg ctgaactcgg cagccgccag gggcgacggc ctgctactgc
     2401 tgggcacccg cgcggccacg cttggtggcg gcggcggtgg cctgagggag agccgccggg
     2461 gcaagcaggg ggcccggatg agcctgctgg ggaagccgct ctcttacacg agtagccaga
     2521 gctgccggcg caacgtcaag taccggcggg tgcagaacta cctgtacaac gtgctggaga
     2581 gaccccgcgg ctgggcgttc atctaccacg ctttcgtttt tctccttgtc tttggttgct
     2641 tgattttgtc agtgttttct accatccctg agcacacaaa attggcctca agttgcctct
     2701 tgatcctgga gttcgtgatg attgtcgtct ttggtttgga gttcatcatt cgaatctggt
     2761 ctgcgggttg ctgttgtcga tatagaggat ggcaaggaag actgaggttt gctcgaaagc
     2821 ccttctgtgt tatagatacc attgttctta tcgcttcaat agcagttgtt tctgcaaaaa
     2881 ctcagggtaa tatttttgcc acgtctgcac tcagaagtct ccgtttccta cagatcctcc
     2941 gcatggtgcg catggaccga aggggaggca cttggaaatt actgggttca gtggtttatg
     3001 ctcacagcaa ggaattaatc acagcttggt acataggatt tttggttctt attttttcgt
     3061 ctttccttgt ctatctggtg gaaaaggatg ccaataaaga gttttctaca tatgcagatg
     3121 ctctctggtg gggcacaatt acattgacaa ctattggcta tggagacaaa actcccctaa
     3181 cttggctggg aagattgctt tctgcaggct ttgcactcct tggcatttct ttctttgcac
     3241 ttcctgccgg cattcttggc tcaggttttg cattaaaagt acaagaacaa caccgccaga
     3301 aacactttga gaaaagaagg aacccagctg ccaacctcat tcagtgtgtt tggcgtagtt
     3361 acgcagctga tgagaaatct gtttccattg caacctggaa gccacacttg aaggccttgc
     3421 acacctgcag ccctaccaag aaagaacaag gggaagcatc aagcagttgg ggacagtgga
     3481 cattgcgtca gaagctaagt tttaaggagc aagtgcgcat ggctagcccc aggggccaga
     3541 gtattaagag ccgacaagcc tcagtaggtg acaggaggtc cccaagcacc gacatcacag
     3601 ccgagggcag tcccaccaaa gtgcagaaga gctggagctt caacgaccga acccgcttcc
     3661 ggccctcgct gcgcctcaaa agttctcagc caaaaccagt gatagatgct gacacagccc
     3721 ttggcactga tgatgtatat gatgaaaaag gatgccagtg tgatgtatca gtggaagacc
     3781 tcaccccacc acttaaaact gtcattcgag ctatcagaat tatgaaattt catgttgcaa
     3841 aacggaagtt taaggaaaca ttacgtccat atgatgtaaa agatgtcatt gaacaatatt
     3901 ctgctggtca tctggacatg ttgtgtagaa ttaaaagcct tcaaacacgt gttgatcaaa
     3961 ttcttggaaa agggcaaatc acatcagata agaagagccg agagaaaata acagcagaac
     4021 atgagaccac agacgatctc agtatgctcg gtcgggtggt caaggttgaa aaacaggtac
     4081 agtccataga atccaagctg gactgcctac tagacatcta tcaacaggtc cttcggaaag
     4141 gctctgcctc agccctcgct ttggcttcat tccagatccc accttttgaa tgtgaacaga
     4201 catctgacta tcaaagccct gtggatagca aagatctttc gggttccgca caaaacagtg
     4261 gctgcttatc cagatcaact agtgccaaca tctcgagagg cctgcagttc attctgacgc
     4321 caaatgagtt cagtgcccag actttctacg cgcttagccc tactatgcac agtcaagcaa
     4381 cacaggtgcc aattagtcaa agcgatggct cagcagtggc agccaccaac accattgcaa
     4441 accaaataaa tacggcaccc aagccagcag ccccaacaac tttacagatc ccacctcctc
     4501 tcccagccat caagcatctg cccaggccag aaactctgca ccctaaccct gcaggcttac
     4561 aggaaagcat ttctgacgtc accacctgcc ttgttgcctc caaggaaaat gttcaggttg
     4621 cacagtcaaa tctcaccaag gaccgttcta tgaggaaaag ctttgacatg ggaggagaaa
     4681 ctctgttgtc tgtctgtccc atggtgccga aggacttggg caaatctttg tctgtgcaaa
     4741 acctgatcag gtcgaccgag gaactgaata tacaactttc agggagtgag tcaagtggct
     4801 ccagaggcag ccaagatttt taccccaaat ggagggaatc caaattgttt ataactgatg
     4861 aagaggtggg tcccgaagag acagagacag acacttttga tgccgcaccg cagcctgcca
     4921 gggaagctgc ctttgcatca gactctctaa ggactggaag gtcacgatca tctcagagca
     4981 tttgtaaggc aggagaaagt acagatgccc tcagcttgcc tcatgtcaaa ctgaaataag
     5041 ttcttcattt tctttccagg catagcagtt ctttagccat acatatcatt gcatgaacta
     5101 tttcgaaagc ccttctaaaa agttgaaatt gcaagaatcg ggaagaacat gaaaggcagt
     5161 ttataagccc gttacctttt aattgcatga aaatgcatgt ttagggatgg ctaaaattcc
     5221 aaggtgcatc gacattaacc cactcattta gtaatgtacc ttgagttaaa aagcctgaga
     5281 aaccaaacac agctaatgct atggggtgta tgaatatgtc aagtttaggt catttagaag
     5341 atttgacact gtattttgaa attatgggag taaacacctt caaatttcag gcatttctgc
     5401 tttgtgacta aatacaaact acattttcaa gattaggcca taatgtatat ttaaacacaa
     5461 tggctatcaa cagctgctaa taaggtatca actaaagcag aattggggaa taatagaaat
     5521 ggctgcttat ttcaagatat atttgccaac ccattcctat tcagtcattt tattattaat
     5581 gtaatttgaa tgtcaatttg tgtgcttttg gtgatttagc gcccgggatc cgcccctctc
     5641 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     5701 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     5761 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     5821 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     5881 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     5941 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     6001 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     6061 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tacacatgct
     6121 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     6181 ttttcctttg aaaaacacga tgataatatg gccacaacca tggtgagcaa gggcgaggag
     6241 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     6301 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     6361 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     6421 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     6481 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     6541 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     6601 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     6661 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     6721 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     6781 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     6841 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     6901 gccgggatca ctctcggcat ggacgagctg tacaagtaat ctagagggcc ctattctata
     6961 gtgtcaccta aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc
     7021 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     7081 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     7141 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     7201 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     7261 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     7321 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     7381 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     7441 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     7501 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     7561 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     7621 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     7681 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     7741 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     7801 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     7861 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     7921 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
     7981 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     8041 aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg
     8101 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     8161 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     8221 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     8281 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     8341 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     8401 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     8461 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     8521 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     8581 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
     8641 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     8701 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     8761 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     8821 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     8881 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
     8941 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     9001 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     9061 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     9121 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     9181 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     9241 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     9301 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     9361 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     9421 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     9481 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     9541 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     9601 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     9661 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     9721 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     9781 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     9841 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     9901 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     9961 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    10021 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    10081 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    10141 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    10201 agcagcagat tacgcgcaga aaaaaaggat ctcaa
//
