LOCUS       sequence-404015-       10235 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Heterologous expression in mammalian cell lines or Xenopus oocyte 
            (T7 RNA pol).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10235)
  AUTHORS   Wei AD, Wakenight P, Zwingman TA, Bard AM, Sahai N, Willemsen MH, 
            Schelhaas HJ, Stegmann APA, Verhoeven JS, de Man SA, Wessels MW, 
            Kleefstra T, Shinde DN, Helbig KL, Basinger A, Wagner VF, 
            Rodriguez-Buritica D, Bryant E, Millichap JJ, Millen KJ, Dobyns WB, 
            Ramirez JM, Kalume FK
  TITLE     Human KCNQ5 de novo mutations underlie epilepsy and intellectual 
            disability.
  JOURNAL   J Neurophysiol. 2022 Jul 1;128(1):40-61. doi: 10.1152/jn.00509.2021.
            Epub 2022 May 18.
  PUBMED    35583973
REFERENCE   2  (bases 1 to 10235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1152/jn.00509.2021"; journalName: "J Neurophysiol"; date: 
            "2022-07-1- 1"; volume: "128"; issue: "1"; pages: "40-61"
COMMENT     Sequence Label: hKCNQ5(H576R).ires.GFP-pcDNA3
FEATURES             Location/Qualifiers
     source          1..10235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(159..176)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     386..405
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(579..581,582..584,585..1298)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(579..581,582..584,585..1295)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(624..645)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(885..904)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     promoter        complement(1314..1332)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1315..1332)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1352..1369)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1358..1582
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1628..2056
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1715..1734)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1925..1946
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2065..2085)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2070..2399
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2250..2385
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2312..2331
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2466..3260
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2520..2539)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3130..3149
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3434..3555
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3471..3490)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3525..3544
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3604..3620)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3604..3620)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3617..3639)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3628..3644
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3652..3658,3659..3676,3677..3682))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3697..3718
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3835..3852)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4006..4594)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4086..4105)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4765..5556,5557..5625))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5388..5407
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5626..5730)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5805..5824)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5996..6375
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6376..6579
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6530..6550
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6624..6643
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6624..6642
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           complement(6681..6723)
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     misc_feature    join(10227..10235,1..566,567..569,570..578)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
ORIGIN
        1 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
       61 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
      121 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
      181 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
      241 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
      301 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
      361 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
      421 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt acacatgctt
      481 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
      541 tttcctttga aaaacacgat gataatatgg ccacaaccat ggtgagcaag ggcgaggagc
      601 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
      661 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
      721 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
      781 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
      841 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
      901 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
      961 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     1021 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     1081 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     1141 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     1201 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     1261 ccgggatcac tctcggcatg gacgagctgt acaagtaatc tagagggccc tattctatag
     1321 tgtcacctaa atgctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca
     1381 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     1441 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     1501 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     1561 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     1621 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     1681 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     1741 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     1801 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     1861 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     1921 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     1981 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     2041 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     2101 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     2161 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     2221 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     2281 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     2341 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     2401 agctcccggg agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt
     2461 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     2521 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     2581 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     2641 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     2701 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     2761 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     2821 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     2881 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     2941 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     3001 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     3061 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     3121 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     3181 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     3241 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     3301 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     3361 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     3421 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     3481 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     3541 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     3601 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     3661 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     3721 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     3781 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     3841 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     3901 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     3961 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     4021 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     4081 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     4141 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     4201 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     4261 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     4321 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     4381 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     4441 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     4501 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     4561 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     4621 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     4681 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     4741 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     4801 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     4861 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     4921 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     4981 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     5041 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     5101 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     5161 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     5221 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     5281 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     5341 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     5401 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     5461 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     5521 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     5581 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     5641 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     5701 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     5761 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
     5821 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
     5881 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
     5941 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
     6001 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     6061 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     6121 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     6181 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     6241 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     6301 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     6361 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     6421 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     6481 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     6541 ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc
     6601 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agcttatcga
     6661 taccgtcgac gctagctctg ccaaagttga gcgtttattc tgagcttctg caaaaagaac
     6721 aagaagggta ccgagctcgg atccactagt aacggccgcc agtgtgctgg aattctgcag
     6781 atatccatca cactggcggc cgctctagat gccatgcccc gccaccacgc gggaggagag
     6841 gagggcggcg ccgccgggct ctgggtgaag agcggcgcag cggcggcggc ggcgggcggg
     6901 gggcgcttgg gcagcggcat gaaggatgtg gagtcgggcc ggggcagggt gctgctgaac
     6961 tcggcagccg ccaggggcga cggcctgcta ctgctgggca cccgcgcggc cacgcttggt
     7021 ggcggcggcg gtggcctgag ggagagccgc cggggcaagc agggggcccg gatgagcctg
     7081 ctggggaagc cgctctctta cacgagtagc cagagctgcc ggcgcaacgt caagtaccgg
     7141 cgggtgcaga actacctgta caacgtgctg gagagacccc gcggctgggc gttcatctac
     7201 cacgctttcg tttttctcct tgtctttggt tgcttgattt tgtcagtgtt ttctaccatc
     7261 cctgagcaca caaaattggc ctcaagttgc ctcttgatcc tggagttcgt gatgattgtc
     7321 gtctttggtt tggagttcat cattcgaatc tggtctgcgg gttgctgttg tcgatataga
     7381 ggatggcaag gaagactgag gtttgctcga aagcccttct gtgttataga taccattgtt
     7441 cttatcgctt caatagcagt tgtttctgca aaaactcagg gtaatatttt tgccacgtct
     7501 gcactcagaa gtctccgttt cctacagatc ctccgcatgg tgcgcatgga ccgaagggga
     7561 ggcacttgga aattactggg ttcagtggtt tatgctcaca gcaaggaatt aatcacagct
     7621 tggtacatag gatttttggt tcttattttt tcgtctttcc ttgtctatct ggtggaaaag
     7681 gatgccaata aagagttttc tacatatgca gatgctctct ggtggggcac aattacattg
     7741 acaactattg gctatggaga caaaactccc ctaacttggc tgggaagatt gctttctgca
     7801 ggctttgcac tccttggcat ttctttcttt gcacttcctg ccggcattct tggctcaggt
     7861 tttgcattaa aagtacaaga acaacaccgc cagaaacact ttgagaaaag aaggaaccca
     7921 gctgccaacc tcattcagtg tgtttggcgt agttacgcag ctgatgagaa atctgtttcc
     7981 attgcaacct ggaagccaca cttgaaggcc ttgcacacct gcagccctac caagaaagaa
     8041 caaggggaag catcaagcag ttggggacag tggacattgc gtcagaagct aagttttaag
     8101 gagcgagtgc gcatggctag ccccaggggc cagagtatta agagccgaca agcctcagta
     8161 ggtgacagga ggtccccaag caccgacatc acagccgagg gcagtcccac caaagtgcag
     8221 aagagctgga gcttcaacga ccgaacccgc ttccggccct cgctgcgcct caaaagttct
     8281 cagccaaaac cagtgataga tgctgacaca gcccttggca ctgatgatgt atatgatgaa
     8341 aaaggatgcc agtgtgatgt atcagtggaa gacctcaccc caccacttaa aactgtcatt
     8401 cgagctatca gaattatgaa atttcatgtt gcaaaacgga agtttaagga aacattacgt
     8461 ccatatgatg taaaagatgt cattgaacaa tattctgctg gtcgtctgga catgttgtgt
     8521 agaattaaaa gccttcaaac acgtgttgat caaattcttg gaaaagggca aatcacatca
     8581 gataagaaga gccgagagaa aataacagca gaacatgaga ccacagacga tctcagtatg
     8641 ctcggtcggg tggtcaaggt tgaaaaacag gtacagtcca tagaatccaa gctggactgc
     8701 ctactagaca tctatcaaca ggtccttcgg aaaggctctg cctcagccct cgctttggct
     8761 tcattccaga tcccaccttt tgaatgtgaa cagacatctg actatcaaag ccctgtggat
     8821 agcaaagatc tttcgggttc cgcacaaaac agtggctgct tatccagatc aactagtgcc
     8881 aacatctcga gaggcctgca gttcattctg acgccaaatg agttcagtgc ccagactttc
     8941 tacgcgctta gccctactat gcacagtcaa gcaacacagg tgccaattag tcaaagcgat
     9001 ggctcagcag tggcagccac caacaccatt gcaaaccaaa taaatacggc acccaagcca
     9061 gcagccccaa caactttaca gatcccacct cctctcccag ccatcaagca tctgcccagg
     9121 ccagaaactc tgcaccctaa ccctgcaggc ttacaggaaa gcatttctga cgtcaccacc
     9181 tgccttgttg cctccaagga aaatgttcag gttgcacagt caaatctcac caaggaccgt
     9241 tctatgagga aaagctttga catgggagga gaaactctgt tgtctgtctg tcccatggtg
     9301 ccgaaggact tgggcaaatc tttgtctgtg caaaacctga tcaggtcgac cgaggaactg
     9361 aatatacaac tttcagggag tgagtcaagt ggctccagag gcagccaaga tttttacccc
     9421 aaatggaggg aatccaaatt gtttataact gatgaagagg tgggtcccga agagacagag
     9481 acagacactt ttgatgccgc accgcagcct gccagggaag ctgcctttgc atcagactct
     9541 ctaaggactg gaaggtcacg atcatctcag agcatttgta aggcaggaga aagtacagat
     9601 gccctcagct tgcctcatgt caaactgaaa taagttcttc attttctttc caggcatagc
     9661 agttctttag ccatacatat cattgcatga actatttcga aagcccttct aaaaagttga
     9721 aattgcaaga atcgggaaga acatgaaagg cagtttataa gcccgttacc ttttaattgc
     9781 atgaaaatgc atgtttaggg atggctaaaa ttccaaggtg catcgacatt aacccactca
     9841 tttagtaatg taccttgagt taaaaagcct gagaaaccaa acacagctaa tgctatgggg
     9901 tgtatgaata tgtcaagttt aggtcattta gaagatttga cactgtattt tgaaattatg
     9961 ggagtaaaca ccttcaaatt tcaggcattt ctgctttgtg actaaataca aactacattt
    10021 tcaagattag gccataatgt atatttaaac acaatggcta tcaacagctg ctaataaggt
    10081 atcaactaaa gcagaattgg ggaataatag aaatggctgc ttatttcaag atatatttgc
    10141 caacccattc ctattcagtc attttattat taatgtaatt tgaatgtcaa tttgtgtgct
    10201 tttggtgatt tagcgcccgg gatccgcccc tctcc
//
