LOCUS       sequence-404016-       10226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Heterologous expression in mammalian cell lines or Xenopus oocyte 
            (T7 RNA pol).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10226)
  AUTHORS   Wei AD, Wakenight P, Zwingman TA, Bard AM, Sahai N, Willemsen MH, 
            Schelhaas HJ, Stegmann APA, Verhoeven JS, de Man SA, Wessels MW, 
            Kleefstra T, Shinde DN, Helbig KL, Basinger A, Wagner VF, 
            Rodriguez-Buritica D, Bryant E, Millichap JJ, Millen KJ, Dobyns WB, 
            Ramirez JM, Kalume FK
  TITLE     Human KCNQ5 de novo mutations underlie epilepsy and intellectual 
            disability.
  JOURNAL   J Neurophysiol. 2022 Jul 1;128(1):40-61. doi: 10.1152/jn.00509.2021.
            Epub 2022 May 18.
  PUBMED    35583973
REFERENCE   2  (bases 1 to 10226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1152/jn.00509.2021"; journalName: "J Neurophysiol"; date: 
            "2022-07-1- 1"; volume: "128"; issue: "1"; pages: "40-61"
COMMENT     Sequence Label: hKCNQ5(V145G).ires.GFP-pcDNA3
FEATURES             Location/Qualifiers
     source          1..10226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(402..976,977..979,980..988)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(569..586)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     796..815
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(989..991,992..994,995..1708)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(989..991,992..994,995..1705)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1034..1055)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1295..1314)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     promoter        complement(1724..1742)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1725..1742)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1762..1779)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1768..1992
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2038..2466
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2125..2144)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2335..2356
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2475..2495)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2480..2809
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2660..2795
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2722..2741
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2876..3670
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2930..2949)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3540..3559
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3844..3965
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3881..3900)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3935..3954
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4014..4030)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4014..4030)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4027..4049)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4038..4054
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4062..4068,4069..4086,4087..4092))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4107..4128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4245..4262)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4416..5004)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4496..4515)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5175..5966,5967..6035))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5798..5817
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6036..6140)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6215..6234)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6406..6785
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6786..6989
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6940..6960
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7034..7053
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7034..7052
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           complement(7091..7133)
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     regulatory      7195..7204
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 acattaaccc actcatttag taatgtacct tgagttaaaa agcctgagaa accaaacaca
       61 gctaatgcta tggggtgtat gaatatgtca agtttaggtc atttagaaga tttgacactg
      121 tattttgaaa ttatgggagt aaacaccttc aaatttcagg catttctgct ttgtgactaa
      181 atacaaacta cattttcaag attaggccat aatgtatatt taaacacaat ggctatcaac
      241 agctgctaat aaggtatcaa ctaaagcaga attggggaat aatagaaatg gctgcttatt
      301 tcaagatata tttgccaacc cattcctatt cagtcatttt attattaatg taatttgaat
      361 gtcaatttgt gtgcttttgg tgatttagcg cccgggatcc gcccctctcc ctcccccccc
      421 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
      481 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
      541 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
      601 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
      661 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
      721 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
      781 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
      841 aaggtacccc attgtatggg atctgatctg gggcctcggt acacatgctt tacatgtgtt
      901 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
      961 aaaacacgat gataatatgg ccacaaccat ggtgagcaag ggcgaggagc tgttcaccgg
     1021 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     1081 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     1141 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     1201 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     1261 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     1321 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     1381 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     1441 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     1501 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     1561 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     1621 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     1681 tctcggcatg gacgagctgt acaagtaatc tagagggccc tattctatag tgtcacctaa
     1741 atgctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     1801 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     1861 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     1921 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     1981 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     2041 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     2101 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     2161 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     2221 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     2281 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     2341 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     2401 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     2461 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     2521 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     2581 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     2641 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     2701 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     2761 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     2821 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat
     2881 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     2941 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     3001 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     3061 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     3121 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     3181 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     3241 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     3301 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     3361 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     3421 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     3481 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     3541 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     3601 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     3661 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     3721 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     3781 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     3841 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     3901 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     3961 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     4021 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     4081 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     4141 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     4201 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     4261 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     4321 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     4381 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     4441 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     4501 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     4561 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     4621 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     4681 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     4741 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     4801 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     4861 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     4921 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     4981 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     5041 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     5101 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     5161 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     5221 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     5281 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     5341 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     5401 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     5461 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     5521 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     5581 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     5641 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     5701 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     5761 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     5821 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     5881 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     5941 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     6001 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     6061 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     6121 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
     6181 ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     6241 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
     6301 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
     6361 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga
     6421 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     6481 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     6541 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     6601 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     6661 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     6721 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     6781 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     6841 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     6901 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     6961 tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact
     7021 ggcttatcga aattaatacg actcactata gggagaccca agcttatcga taccgtcgac
     7081 gctagctctg ccaaagttga gcgtttattc tgagcttctg caaaaagaac aagaagggta
     7141 ccgagctcgg atccactagt aacggccgcc agtgtgctgg aattctgcag atatccatgg
     7201 cggccgccgc cgccatgccc cgccaccacg cgggaggaga ggagggcggc gccgccgggc
     7261 tctgggtgaa gagcggcgca gcggcggcgg cggcgggcgg ggggcgcttg ggcagcggca
     7321 tgaaggatgt ggagtcgggc cggggcaggg tgctgctgaa ctcggcagcc gccaggggcg
     7381 acggcctgct actgctgggc acccgcgcgg ccacgcttgg tggcggcggc ggtggcctga
     7441 gggagagccg ccggggcaag cagggggccc ggatgagcct gctggggaag ccgctctctt
     7501 acacgagtag ccagagctgc cggcgcaacg tcaagtaccg gcgggtgcag aactacctgt
     7561 acaacgtgct ggagagaccc cgcggctggg cgttcatcta ccacgctttc gtttttctcc
     7621 ttgtctttgg ttgcttgatt ttgtcagggt tttctaccat ccctgagcac acaaaattgg
     7681 cctcaagttg cctcttgatc ctggagttcg tgatgattgt cgtctttggt ttggagttca
     7741 tcattcgaat ctggtctgcg ggttgctgtt gtcgatatag aggatggcaa ggaagactga
     7801 ggtttgctcg aaagcccttc tgtgttatag ataccattgt tcttatcgct tcaatagcag
     7861 ttgtttctgc aaaaactcag ggtaatattt ttgccacgtc tgcactcaga agtctccgtt
     7921 tcctacagat cctccgcatg gtgcgcatgg accgaagggg aggcacttgg aaattactgg
     7981 gttcagtggt ttatgctcac agcaaggaat taatcacagc ttggtacata ggatttttgg
     8041 ttcttatttt ttcgtctttc cttgtctatc tggtggaaaa ggatgccaat aaagagtttt
     8101 ctacatatgc agatgctctc tggtggggca caattacatt gacaactatt ggctatggag
     8161 acaaaactcc cctaacttgg ctgggaagat tgctttctgc aggctttgca ctccttggca
     8221 tttctttctt tgcacttcct gccggcattc ttggctcagg ttttgcatta aaagtacaag
     8281 aacaacaccg ccagaaacac tttgagaaaa gaaggaaccc agctgccaac ctcattcagt
     8341 gtgtttggcg tagttacgca gctgatgaga aatctgtttc cattgcaacc tggaagccac
     8401 acttgaaggc cttgcacacc tgcagcccta ccaagaaaga acaaggggaa gcatcaagca
     8461 gttggggaca gtggacattg cgtcagaagc taagttttaa ggagcgagtg cgcatggcta
     8521 gccccagggg ccagagtatt aagagccgac aagcctcagt aggtgacagg aggtccccaa
     8581 gcaccgacat cacagccgag ggcagtccca ccaaagtgca gaagagctgg agcttcaacg
     8641 accgaacccg cttccggccc tcgctgcgcc tcaaaagttc tcagccaaaa ccagtgatag
     8701 atgctgacac agcccttggc actgatgatg tatatgatga aaaaggatgc cagtgtgatg
     8761 tatcagtgga agacctcacc ccaccactta aaactgtcat tcgagctatc agaattatga
     8821 aatttcatgt tgcaaaacgg aagtttaagg aaacattacg tccatatgat gtaaaagatg
     8881 tcattgaaca atattctgct ggtcatctgg acatgttgtg tagaattaaa agccttcaaa
     8941 cacgtgttga tcaaattctt ggaaaagggc aaatcacatc agataagaag agccgagaga
     9001 aaataacagc agaacatgag accacagacg atctcagtat gctcggtcgg gtggtcaagg
     9061 ttgaaaaaca ggtacagtcc atagaatcca agctggactg cctactagac atctatcaac
     9121 aggtccttcg gaaaggctct gcctcagccc tcgctttggc ttcattccag atcccacctt
     9181 ttgaatgtga acagacatct gactatcaaa gccctgtgga tagcaaagat ctttcgggtt
     9241 ccgcacaaaa cagtggctgc ttatccagat caactagtgc caacatctcg agaggcctgc
     9301 agttcattct gacgccaaat gagttcagtg cccagacttt ctacgcgctt agccctacta
     9361 tgcacagtca agcaacacag gtgccaatta gtcaaagcga tggctcagca gtggcagcca
     9421 ccaacaccat tgcaaaccaa ataaatacgg cacccaagcc agcagcccca acaactttac
     9481 agatcccacc tcctctccca gccatcaagc atctgcccag gccagaaact ctgcacccta
     9541 accctgcagg cttacaggaa agcatttctg acgtcaccac ctgccttgtt gcctccaagg
     9601 aaaatgttca ggttgcacag tcaaatctca ccaaggaccg ttctatgagg aaaagctttg
     9661 acatgggagg agaaactctg ttgtctgtct gtcccatggt gccgaaggac ttgggcaaat
     9721 ctttgtctgt gcaaaacctg atcaggtcga ccgaggaact gaatatacaa ctttcaggga
     9781 gtgagtcaag tggctccaga ggcagccaag atttttaccc caaatggagg gaatccaaat
     9841 tgtttataac tgatgaagag gtgggtcccg aagagacaga gacagacact tttgatgccg
     9901 caccgcagcc tgccagggaa gctgcctttg catcagactc tctaaggact ggaaggtcac
     9961 gatcatctca gagcatttgt aaggcaggag aaagtacaga tgccctcagc ttgcctcatg
    10021 tcaaactgaa ataagttctt cattttcttt ccaggcatag cagttcttta gccatacata
    10081 tcattgcatg aactatttcg aaagcccttc taaaaagttg aaattgcaag aatcgggaag
    10141 aacatgaaag gcagtttata agcccgttac cttttaattg catgaaaatg catgtttagg
    10201 gatggctaaa attccaaggt gcatcg
//
