LOCUS       sequence-404017-       10226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Heterologous expression in mammalian cell lines or Xenopus oocyte 
            (T7 RNA pol).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10226)
  AUTHORS   Wei AD, Wakenight P, Zwingman TA, Bard AM, Sahai N, Willemsen MH, 
            Schelhaas HJ, Stegmann APA, Verhoeven JS, de Man SA, Wessels MW, 
            Kleefstra T, Shinde DN, Helbig KL, Basinger A, Wagner VF, 
            Rodriguez-Buritica D, Bryant E, Millichap JJ, Millen KJ, Dobyns WB, 
            Ramirez JM, Kalume FK
  TITLE     Human KCNQ5 de novo mutations underlie epilepsy and intellectual 
            disability.
  JOURNAL   J Neurophysiol. 2022 Jul 1;128(1):40-61. doi: 10.1152/jn.00509.2021.
            Epub 2022 May 18.
  PUBMED    35583973
REFERENCE   2  (bases 1 to 10226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1152/jn.00509.2021"; journalName: "J Neurophysiol"; date: 
            "2022-07-1- 1"; volume: "128"; issue: "1"; pages: "40-61"
COMMENT     Sequence Label: hKCNQ5(L341I).ires.GFP-pcDNA3
FEATURES             Location/Qualifiers
     source          1..10226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(157..174)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     384..403
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(577..579,580..582,583..1296)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(577..579,580..582,583..1293)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(622..643)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(883..902)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     promoter        complement(1312..1330)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1313..1330)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1350..1367)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1356..1580
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1626..2054
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1713..1732)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1923..1944
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2063..2083)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2068..2397
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2248..2383
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2310..2329
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2464..3258
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2518..2537)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3128..3147
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3432..3553
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3469..3488)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3523..3542
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3602..3618)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3602..3618)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3615..3637)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3626..3642
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3650..3656,3657..3674,3675..3680))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3695..3716
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3833..3850)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4004..4592)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4084..4103)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4763..5554,5555..5623))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5386..5405
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5624..5728)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5803..5822)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5994..6373
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6374..6577
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6528..6548
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6622..6641
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6622..6640
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           complement(6679..6721)
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     regulatory      6783..6792
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(10216..10226,1..564,565..567,568..576)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
ORIGIN
        1 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
       61 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
      121 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
      181 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
      241 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
      301 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
      361 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
      421 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtac acatgcttta
      481 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
      541 tcctttgaaa aacacgatga taatatggcc acaaccatgg tgagcaaggg cgaggagctg
      601 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
      661 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
      721 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
      781 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
      841 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
      901 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
      961 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     1021 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     1081 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     1141 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     1201 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     1261 gggatcactc tcggcatgga cgagctgtac aagtaatcta gagggcccta ttctatagtg
     1321 tcacctaaat gctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     1381 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     1441 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     1501 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     1561 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     1621 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     1681 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     1741 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     1801 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     1861 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     1921 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     1981 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     2041 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     2101 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     2161 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     2221 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     2281 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     2341 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     2401 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt
     2461 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     2521 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     2581 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     2641 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     2701 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     2761 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     2821 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     2881 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     2941 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     3001 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     3061 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     3121 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     3181 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     3241 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     3301 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     3361 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     3421 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     3481 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     3541 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     3601 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     3661 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     3721 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     3781 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     3841 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     3901 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     3961 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     4021 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     4081 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     4141 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     4201 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     4261 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     4321 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     4381 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     4441 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     4501 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     4561 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     4621 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     4681 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     4741 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     4801 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     4861 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     4921 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     4981 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     5041 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     5101 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     5161 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     5221 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     5281 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     5341 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     5401 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     5461 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     5521 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     5581 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     5641 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     5701 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     5761 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
     5821 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
     5881 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
     5941 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
     6001 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     6061 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     6121 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     6181 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     6241 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     6301 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     6361 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     6421 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     6481 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     6541 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
     6601 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag cttatcgata
     6661 ccgtcgacgc tagctctgcc aaagttgagc gtttattctg agcttctgca aaaagaacaa
     6721 gaagggtacc gagctcggat ccactagtaa cggccgccag tgtgctggaa ttctgcagat
     6781 atccatggcg gccgccgccg ccatgccccg ccaccacgcg ggaggagagg agggcggcgc
     6841 cgccgggctc tgggtgaaga gcggcgcagc ggcggcggcg gcgggcgggg ggcgcttggg
     6901 cagcggcatg aaggatgtgg agtcgggccg gggcagggtg ctgctgaact cggcagccgc
     6961 caggggcgac ggcctgctac tgctgggcac ccgcgcggcc acgcttggtg gcggcggcgg
     7021 tggcctgagg gagagccgcc ggggcaagca gggggcccgg atgagcctgc tggggaagcc
     7081 gctctcttac acgagtagcc agagctgccg gcgcaacgtc aagtaccggc gggtgcagaa
     7141 ctacctgtac aacgtgctgg agagaccccg cggctgggcg ttcatctacc acgctttcgt
     7201 ttttctcctt gtctttggtt gcttgatttt gtcagtgttt tctaccatcc ctgagcacac
     7261 aaaattggcc tcaagttgcc tcttgatcct ggagttcgtg atgattgtcg tctttggttt
     7321 ggagttcatc attcgaatct ggtctgcggg ttgctgttgt cgatatagag gatggcaagg
     7381 aagactgagg tttgctcgaa agcccttctg tgttatagat accattgttc ttatcgcttc
     7441 aatagcagtt gtttctgcaa aaactcaggg taatattttt gccacgtctg cactcagaag
     7501 tctccgtttc ctacagatcc tccgcatggt gcgcatggac cgaaggggag gcacttggaa
     7561 attactgggt tcagtggttt atgctcacag caaggaatta atcacagctt ggtacatagg
     7621 atttttggtt cttatttttt cgtctttcct tgtctatctg gtggaaaagg atgccaataa
     7681 agagttttct acatatgcag atgctctctg gtggggcaca attacattga caactattgg
     7741 ctatggagac aaaactcccc taacttggct gggaagattg ctttctgcag gctttgcact
     7801 ccttggcatt tctttctttg caattcctgc cggcattctt ggctcaggtt ttgcattaaa
     7861 agtacaagaa caacaccgcc agaaacactt tgagaaaaga aggaacccag ctgccaacct
     7921 cattcagtgt gtttggcgta gttacgcagc tgatgagaaa tctgtttcca ttgcaacctg
     7981 gaagccacac ttgaaggcct tgcacacctg cagccctacc aagaaagaac aaggggaagc
     8041 atcaagcagt tggggacagt ggacattgcg tcagaagcta agttttaagg agcgagtgcg
     8101 catggctagc cccaggggcc agagtattaa gagccgacaa gcctcagtag gtgacaggag
     8161 gtccccaagc accgacatca cagccgaggg cagtcccacc aaagtgcaga agagctggag
     8221 cttcaacgac cgaacccgct tccggccctc gctgcgcctc aaaagttctc agccaaaacc
     8281 agtgatagat gctgacacag cccttggcac tgatgatgta tatgatgaaa aaggatgcca
     8341 gtgtgatgta tcagtggaag acctcacccc accacttaaa actgtcattc gagctatcag
     8401 aattatgaaa tttcatgttg caaaacggaa gtttaaggaa acattacgtc catatgatgt
     8461 aaaagatgtc attgaacaat attctgctgg tcatctggac atgttgtgta gaattaaaag
     8521 ccttcaaaca cgtgttgatc aaattcttgg aaaagggcaa atcacatcag ataagaagag
     8581 ccgagagaaa ataacagcag aacatgagac cacagacgat ctcagtatgc tcggtcgggt
     8641 ggtcaaggtt gaaaaacagg tacagtccat agaatccaag ctggactgcc tactagacat
     8701 ctatcaacag gtccttcgga aaggctctgc ctcagccctc gctttggctt cattccagat
     8761 cccacctttt gaatgtgaac agacatctga ctatcaaagc cctgtggata gcaaagatct
     8821 ttcgggttcc gcacaaaaca gtggctgctt atccagatca actagtgcca acatctcgag
     8881 aggcctgcag ttcattctga cgccaaatga gttcagtgcc cagactttct acgcgcttag
     8941 ccctactatg cacagtcaag caacacaggt gccaattagt caaagcgatg gctcagcagt
     9001 ggcagccacc aacaccattg caaaccaaat aaatacggca cccaagccag cagccccaac
     9061 aactttacag atcccacctc ctctcccagc catcaagcat ctgcccaggc cagaaactct
     9121 gcaccctaac cctgcaggct tacaggaaag catttctgac gtcaccacct gccttgttgc
     9181 ctccaaggaa aatgttcagg ttgcacagtc aaatctcacc aaggaccgtt ctatgaggaa
     9241 aagctttgac atgggaggag aaactctgtt gtctgtctgt cccatggtgc cgaaggactt
     9301 gggcaaatct ttgtctgtgc aaaacctgat caggtcgacc gaggaactga atatacaact
     9361 ttcagggagt gagtcaagtg gctccagagg cagccaagat ttttacccca aatggaggga
     9421 atccaaattg tttataactg atgaagaggt gggtcccgaa gagacagaga cagacacttt
     9481 tgatgccgca ccgcagcctg ccagggaagc tgcctttgca tcagactctc taaggactgg
     9541 aaggtcacga tcatctcaga gcatttgtaa ggcaggagaa agtacagatg ccctcagctt
     9601 gcctcatgtc aaactgaaat aagttcttca ttttctttcc aggcatagca gttctttagc
     9661 catacatatc attgcatgaa ctatttcgaa agcccttcta aaaagttgaa attgcaagaa
     9721 tcgggaagaa catgaaaggc agtttataag cccgttacct tttaattgca tgaaaatgca
     9781 tgtttaggga tggctaaaat tccaaggtgc atcgacatta acccactcat ttagtaatgt
     9841 accttgagtt aaaaagcctg agaaaccaaa cacagctaat gctatggggt gtatgaatat
     9901 gtcaagttta ggtcatttag aagatttgac actgtatttt gaaattatgg gagtaaacac
     9961 cttcaaattt caggcatttc tgctttgtga ctaaatacaa actacatttt caagattagg
    10021 ccataatgta tatttaaaca caatggctat caacagctgc taataaggta tcaactaaag
    10081 cagaattggg gaataataga aatggctgct tatttcaaga tatatttgcc aacccattcc
    10141 tattcagtca ttttattatt aatgtaattt gaatgtcaat ttgtgtgctt ttggtgattt
    10201 agcgcccggg atccgcccct ctccct
//
