LOCUS       sequence-404018-       10226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Heterologous expression in mammalian cell lines or Xenopus oocyte 
            (T7 RNA pol).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10226)
  AUTHORS   Wei AD, Wakenight P, Zwingman TA, Bard AM, Sahai N, Willemsen MH, 
            Schelhaas HJ, Stegmann APA, Verhoeven JS, de Man SA, Wessels MW, 
            Kleefstra T, Shinde DN, Helbig KL, Basinger A, Wagner VF, 
            Rodriguez-Buritica D, Bryant E, Millichap JJ, Millen KJ, Dobyns WB, 
            Ramirez JM, Kalume FK
  TITLE     Human KCNQ5 de novo mutations underlie epilepsy and intellectual 
            disability.
  JOURNAL   J Neurophysiol. 2022 Jul 1;128(1):40-61. doi: 10.1152/jn.00509.2021.
            Epub 2022 May 18.
  PUBMED    35583973
REFERENCE   2  (bases 1 to 10226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1152/jn.00509.2021"; journalName: "J Neurophysiol"; date: 
            "2022-07-1- 1"; volume: "128"; issue: "1"; pages: "40-61"
COMMENT     Sequence Label: hKCNQ5(P369R).ires.GFP-pcDNA3
FEATURES             Location/Qualifiers
     source          1..10226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(153..170)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     380..399
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(573..575,576..578,579..1292)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(573..575,576..578,579..1289)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(618..639)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(879..898)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     promoter        complement(1308..1326)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1309..1326)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1346..1363)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1352..1576
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1622..2050
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1709..1728)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1919..1940
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2059..2079)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2064..2393
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2244..2379
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2306..2325
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2460..3254
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2514..2533)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3124..3143
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3428..3549
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3465..3484)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3519..3538
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3598..3614)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3598..3614)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3611..3633)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3622..3638
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3646..3652,3653..3670,3671..3676))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3691..3712
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3829..3846)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4000..4588)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4080..4099)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4759..5550,5551..5619))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5382..5401
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5620..5724)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5799..5818)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5990..6369
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6370..6573
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6524..6544
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6618..6637
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6618..6636
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           complement(6675..6717)
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     regulatory      6779..6788
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(10212..10226,1..560,561..563,564..572)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
ORIGIN
        1 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
       61 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
      121 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
      181 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
      241 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
      301 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
      361 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
      421 ccagaaggta ccccattgta tgggatctga tctggggcct cggtacacat gctttacatg
      481 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
      541 ttgaaaaaca cgatgataat atggccacaa ccatggtgag caagggcgag gagctgttca
      601 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      661 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      721 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
      781 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      841 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
      901 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      961 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     1021 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     1081 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     1141 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     1201 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     1261 tcactctcgg catggacgag ctgtacaagt aatctagagg gccctattct atagtgtcac
     1321 ctaaatgcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     1381 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     1441 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     1501 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     1561 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     1621 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     1681 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     1741 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     1801 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     1861 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     1921 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     1981 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     2041 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     2101 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     2161 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     2221 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     2281 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     2341 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     2401 cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca
     2461 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     2521 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     2581 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     2641 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     2701 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     2761 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     2821 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     2881 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     2941 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     3001 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     3061 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     3121 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     3181 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     3241 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     3301 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     3361 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     3421 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3481 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3541 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     3601 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     3661 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     3721 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     3781 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     3841 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     3901 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     3961 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     4021 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     4081 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     4141 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     4201 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     4261 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     4321 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     4381 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     4441 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     4501 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     4561 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     4621 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     4681 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     4741 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     4801 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     4861 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     4921 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     4981 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     5041 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     5101 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     5161 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     5221 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     5281 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     5341 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     5401 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     5461 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     5521 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     5581 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     5641 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     5701 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     5761 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
     5821 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
     5881 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
     5941 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
     6001 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     6061 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     6121 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     6181 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     6241 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     6301 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     6361 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     6421 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     6481 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     6541 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
     6601 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctta tcgataccgt
     6661 cgacgctagc tctgccaaag ttgagcgttt attctgagct tctgcaaaaa gaacaagaag
     6721 ggtaccgagc tcggatccac tagtaacggc cgccagtgtg ctggaattct gcagatatcc
     6781 atggcggccg ccgccgccat gccccgccac cacgcgggag gagaggaggg cggcgccgcc
     6841 gggctctggg tgaagagcgg cgcagcggcg gcggcggcgg gcggggggcg cttgggcagc
     6901 ggcatgaagg atgtggagtc gggccggggc agggtgctgc tgaactcggc agccgccagg
     6961 ggcgacggcc tgctactgct gggcacccgc gcggccacgc ttggtggcgg cggcggtggc
     7021 ctgagggaga gccgccgggg caagcagggg gcccggatga gcctgctggg gaagccgctc
     7081 tcttacacga gtagccagag ctgccggcgc aacgtcaagt accggcgggt gcagaactac
     7141 ctgtacaacg tgctggagag accccgcggc tgggcgttca tctaccacgc tttcgttttt
     7201 ctccttgtct ttggttgctt gattttgtca gtgttttcta ccatccctga gcacacaaaa
     7261 ttggcctcaa gttgcctctt gatcctggag ttcgtgatga ttgtcgtctt tggtttggag
     7321 ttcatcattc gaatctggtc tgcgggttgc tgttgtcgat atagaggatg gcaaggaaga
     7381 ctgaggtttg ctcgaaagcc cttctgtgtt atagatacca ttgttcttat cgcttcaata
     7441 gcagttgttt ctgcaaaaac tcagggtaat atttttgcca cgtctgcact cagaagtctc
     7501 cgtttcctac agatcctccg catggtgcgc atggaccgaa ggggaggcac ttggaaatta
     7561 ctgggttcag tggtttatgc tcacagcaag gaattaatca cagcttggta cataggattt
     7621 ttggttctta ttttttcgtc tttccttgtc tatctggtgg aaaaggatgc caataaagag
     7681 ttttctacat atgcagatgc tctctggtgg ggcacaatta cattgacaac tattggctat
     7741 ggagacaaaa ctcccctaac ttggctggga agattgcttt ctgcaggctt tgcactcctt
     7801 ggcatttctt tctttgcact tcctgccggc attcttggct caggttttgc attaaaagta
     7861 caagaacaac accgccagaa acactttgag aaaagaagga accgagctgc caacctcatt
     7921 cagtgtgttt ggcgtagtta cgcagctgat gagaaatctg tttccattgc aacctggaag
     7981 ccacacttga aggccttgca cacctgcagc cctaccaaga aagaacaagg ggaagcatca
     8041 agcagttggg gacagtggac attgcgtcag aagctaagtt ttaaggagcg agtgcgcatg
     8101 gctagcccca ggggccagag tattaagagc cgacaagcct cagtaggtga caggaggtcc
     8161 ccaagcaccg acatcacagc cgagggcagt cccaccaaag tgcagaagag ctggagcttc
     8221 aacgaccgaa cccgcttccg gccctcgctg cgcctcaaaa gttctcagcc aaaaccagtg
     8281 atagatgctg acacagccct tggcactgat gatgtatatg atgaaaaagg atgccagtgt
     8341 gatgtatcag tggaagacct caccccacca cttaaaactg tcattcgagc tatcagaatt
     8401 atgaaatttc atgttgcaaa acggaagttt aaggaaacat tacgtccata tgatgtaaaa
     8461 gatgtcattg aacaatattc tgctggtcat ctggacatgt tgtgtagaat taaaagcctt
     8521 caaacacgtg ttgatcaaat tcttggaaaa gggcaaatca catcagataa gaagagccga
     8581 gagaaaataa cagcagaaca tgagaccaca gacgatctca gtatgctcgg tcgggtggtc
     8641 aaggttgaaa aacaggtaca gtccatagaa tccaagctgg actgcctact agacatctat
     8701 caacaggtcc ttcggaaagg ctctgcctca gccctcgctt tggcttcatt ccagatccca
     8761 ccttttgaat gtgaacagac atctgactat caaagccctg tggatagcaa agatctttcg
     8821 ggttccgcac aaaacagtgg ctgcttatcc agatcaacta gtgccaacat ctcgagaggc
     8881 ctgcagttca ttctgacgcc aaatgagttc agtgcccaga ctttctacgc gcttagccct
     8941 actatgcaca gtcaagcaac acaggtgcca attagtcaaa gcgatggctc agcagtggca
     9001 gccaccaaca ccattgcaaa ccaaataaat acggcaccca agccagcagc cccaacaact
     9061 ttacagatcc cacctcctct cccagccatc aagcatctgc ccaggccaga aactctgcac
     9121 cctaaccctg caggcttaca ggaaagcatt tctgacgtca ccacctgcct tgttgcctcc
     9181 aaggaaaatg ttcaggttgc acagtcaaat ctcaccaagg accgttctat gaggaaaagc
     9241 tttgacatgg gaggagaaac tctgttgtct gtctgtccca tggtgccgaa ggacttgggc
     9301 aaatctttgt ctgtgcaaaa cctgatcagg tcgaccgagg aactgaatat acaactttca
     9361 gggagtgagt caagtggctc cagaggcagc caagattttt accccaaatg gagggaatcc
     9421 aaattgttta taactgatga agaggtgggt cccgaagaga cagagacaga cacttttgat
     9481 gccgcaccgc agcctgccag ggaagctgcc tttgcatcag actctctaag gactggaagg
     9541 tcacgatcat ctcagagcat ttgtaaggca ggagaaagta cagatgccct cagcttgcct
     9601 catgtcaaac tgaaataagt tcttcatttt ctttccaggc atagcagttc tttagccata
     9661 catatcattg catgaactat ttcgaaagcc cttctaaaaa gttgaaattg caagaatcgg
     9721 gaagaacatg aaaggcagtt tataagcccg ttacctttta attgcatgaa aatgcatgtt
     9781 tagggatggc taaaattcca aggtgcatcg acattaaccc actcatttag taatgtacct
     9841 tgagttaaaa agcctgagaa accaaacaca gctaatgcta tggggtgtat gaatatgtca
     9901 agtttaggtc atttagaaga tttgacactg tattttgaaa ttatgggagt aaacaccttc
     9961 aaatttcagg catttctgct ttgtgactaa atacaaacta cattttcaag attaggccat
    10021 aatgtatatt taaacacaat ggctatcaac agctgctaat aaggtatcaa ctaaagcaga
    10081 attggggaat aatagaaatg gctgcttatt tcaagatata tttgccaacc cattcctatt
    10141 cagtcatttt attattaatg taatttgaat gtcaatttgt gtgcttttgg tgatttagcg
    10201 cccgggatcc gcccctctcc ctcccc
//
