LOCUS       sequence-404019-       10226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Heterologous expression in mammalian cell lines or Xenopus oocyte 
            (T7 RNA pol).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10226)
  AUTHORS   Wei AD, Wakenight P, Zwingman TA, Bard AM, Sahai N, Willemsen MH, 
            Schelhaas HJ, Stegmann APA, Verhoeven JS, de Man SA, Wessels MW, 
            Kleefstra T, Shinde DN, Helbig KL, Basinger A, Wagner VF, 
            Rodriguez-Buritica D, Bryant E, Millichap JJ, Millen KJ, Dobyns WB, 
            Ramirez JM, Kalume FK
  TITLE     Human KCNQ5 de novo mutations underlie epilepsy and intellectual 
            disability.
  JOURNAL   J Neurophysiol. 2022 Jul 1;128(1):40-61. doi: 10.1152/jn.00509.2021.
            Epub 2022 May 18.
  PUBMED    35583973
REFERENCE   2  (bases 1 to 10226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1152/jn.00509.2021"; journalName: "J Neurophysiol"; date: 
            "2022-07-1- 1"; volume: "128"; issue: "1"; pages: "40-61"
COMMENT     Sequence Label: hKCNQ5(S448I).ires.GFP-pcDNA3
FEATURES             Location/Qualifiers
     source          1..10226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(151..168)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     378..397
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(571..573,574..576,577..1290)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(571..573,574..576,577..1287)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(616..637)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(877..896)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     promoter        complement(1306..1324)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1307..1324)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1344..1361)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1350..1574
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1620..2048
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1707..1726)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1917..1938
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2057..2077)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2062..2391
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2242..2377
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2304..2323
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2458..3252
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2512..2531)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3122..3141
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3426..3547
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3463..3482)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3517..3536
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3596..3612)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3596..3612)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3609..3631)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3620..3636
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3644..3650,3651..3668,3669..3674))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3689..3710
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3827..3844)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3998..4586)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4078..4097)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4757..5548,5549..5617))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5380..5399
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5618..5722)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5797..5816)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5988..6367
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6368..6571
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6522..6542
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6616..6635
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6616..6634
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           complement(6673..6715)
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     regulatory      6777..6786
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(10210..10226,1..558,559..561,562..570)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
ORIGIN
        1 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
       61 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
      121 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
      181 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
      241 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
      301 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
      361 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
      421 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtacacatgc tttacatgtg
      481 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
      541 gaaaaacacg atgataatat ggccacaacc atggtgagca agggcgagga gctgttcacc
      601 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
      661 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
      721 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
      781 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
      841 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
      901 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
      961 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     1021 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     1081 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     1141 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     1201 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     1261 actctcggca tggacgagct gtacaagtaa tctagagggc cctattctat agtgtcacct
     1321 aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     1381 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     1441 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     1501 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     1561 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     1621 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     1681 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     1741 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     1801 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     1861 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     1921 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     1981 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     2041 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     2101 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     2161 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     2221 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     2281 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     2341 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     2401 ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg
     2461 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     2521 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     2581 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     2641 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     2701 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     2761 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     2821 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     2881 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     2941 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     3001 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     3061 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     3121 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     3181 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     3241 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
     3301 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     3361 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     3421 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     3481 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     3541 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     3601 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     3661 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     3721 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     3781 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     3841 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     3901 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     3961 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     4021 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     4081 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     4141 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     4201 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     4261 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     4321 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     4381 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     4441 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     4501 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     4561 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     4621 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     4681 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     4741 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     4801 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     4861 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     4921 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     4981 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     5041 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     5101 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     5161 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     5221 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     5281 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     5341 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     5401 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     5461 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     5521 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     5581 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     5641 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     5701 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     5761 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
     5821 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
     5881 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
     5941 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
     6001 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     6061 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     6121 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     6181 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     6241 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     6301 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     6361 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     6421 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     6481 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     6541 tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac ccactgctta
     6601 ctggcttatc gaaattaata cgactcacta tagggagacc caagcttatc gataccgtcg
     6661 acgctagctc tgccaaagtt gagcgtttat tctgagcttc tgcaaaaaga acaagaaggg
     6721 taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattctgc agatatccat
     6781 ggcggccgcc gccgccatgc cccgccacca cgcgggagga gaggagggcg gcgccgccgg
     6841 gctctgggtg aagagcggcg cagcggcggc ggcggcgggc ggggggcgct tgggcagcgg
     6901 catgaaggat gtggagtcgg gccggggcag ggtgctgctg aactcggcag ccgccagggg
     6961 cgacggcctg ctactgctgg gcacccgcgc ggccacgctt ggtggcggcg gcggtggcct
     7021 gagggagagc cgccggggca agcagggggc ccggatgagc ctgctgggga agccgctctc
     7081 ttacacgagt agccagagct gccggcgcaa cgtcaagtac cggcgggtgc agaactacct
     7141 gtacaacgtg ctggagagac cccgcggctg ggcgttcatc taccacgctt tcgtttttct
     7201 ccttgtcttt ggttgcttga ttttgtcagt gttttctacc atccctgagc acacaaaatt
     7261 ggcctcaagt tgcctcttga tcctggagtt cgtgatgatt gtcgtctttg gtttggagtt
     7321 catcattcga atctggtctg cgggttgctg ttgtcgatat agaggatggc aaggaagact
     7381 gaggtttgct cgaaagccct tctgtgttat agataccatt gttcttatcg cttcaatagc
     7441 agttgtttct gcaaaaactc agggtaatat ttttgccacg tctgcactca gaagtctccg
     7501 tttcctacag atcctccgca tggtgcgcat ggaccgaagg ggaggcactt ggaaattact
     7561 gggttcagtg gtttatgctc acagcaagga attaatcaca gcttggtaca taggattttt
     7621 ggttcttatt ttttcgtctt tccttgtcta tctggtggaa aaggatgcca ataaagagtt
     7681 ttctacatat gcagatgctc tctggtgggg cacaattaca ttgacaacta ttggctatgg
     7741 agacaaaact cccctaactt ggctgggaag attgctttct gcaggctttg cactccttgg
     7801 catttctttc tttgcacttc ctgccggcat tcttggctca ggttttgcat taaaagtaca
     7861 agaacaacac cgccagaaac actttgagaa aagaaggaac ccagctgcca acctcattca
     7921 gtgtgtttgg cgtagttacg cagctgatga gaaatctgtt tccattgcaa cctggaagcc
     7981 acacttgaag gccttgcaca cctgcagccc taccaagaaa gaacaagggg aagcatcaag
     8041 cagttgggga cagtggacat tgcgtcagaa gctaagtttt aaggagcgag tgcgcatggc
     8101 tatccccagg ggccagagta ttaagagccg acaagcctca gtaggtgaca ggaggtcccc
     8161 aagcaccgac atcacagccg agggcagtcc caccaaagtg cagaagagct ggagcttcaa
     8221 cgaccgaacc cgcttccggc cctcgctgcg cctcaaaagt tctcagccaa aaccagtgat
     8281 agatgctgac acagcccttg gcactgatga tgtatatgat gaaaaaggat gccagtgtga
     8341 tgtatcagtg gaagacctca ccccaccact taaaactgtc attcgagcta tcagaattat
     8401 gaaatttcat gttgcaaaac ggaagtttaa ggaaacatta cgtccatatg atgtaaaaga
     8461 tgtcattgaa caatattctg ctggtcatct ggacatgttg tgtagaatta aaagccttca
     8521 aacacgtgtt gatcaaattc ttggaaaagg gcaaatcaca tcagataaga agagccgaga
     8581 gaaaataaca gcagaacatg agaccacaga cgatctcagt atgctcggtc gggtggtcaa
     8641 ggttgaaaaa caggtacagt ccatagaatc caagctggac tgcctactag acatctatca
     8701 acaggtcctt cggaaaggct ctgcctcagc cctcgctttg gcttcattcc agatcccacc
     8761 ttttgaatgt gaacagacat ctgactatca aagccctgtg gatagcaaag atctttcggg
     8821 ttccgcacaa aacagtggct gcttatccag atcaactagt gccaacatct cgagaggcct
     8881 gcagttcatt ctgacgccaa atgagttcag tgcccagact ttctacgcgc ttagccctac
     8941 tatgcacagt caagcaacac aggtgccaat tagtcaaagc gatggctcag cagtggcagc
     9001 caccaacacc attgcaaacc aaataaatac ggcacccaag ccagcagccc caacaacttt
     9061 acagatccca cctcctctcc cagccatcaa gcatctgccc aggccagaaa ctctgcaccc
     9121 taaccctgca ggcttacagg aaagcatttc tgacgtcacc acctgccttg ttgcctccaa
     9181 ggaaaatgtt caggttgcac agtcaaatct caccaaggac cgttctatga ggaaaagctt
     9241 tgacatggga ggagaaactc tgttgtctgt ctgtcccatg gtgccgaagg acttgggcaa
     9301 atctttgtct gtgcaaaacc tgatcaggtc gaccgaggaa ctgaatatac aactttcagg
     9361 gagtgagtca agtggctcca gaggcagcca agatttttac cccaaatgga gggaatccaa
     9421 attgtttata actgatgaag aggtgggtcc cgaagagaca gagacagaca cttttgatgc
     9481 cgcaccgcag cctgccaggg aagctgcctt tgcatcagac tctctaagga ctggaaggtc
     9541 acgatcatct cagagcattt gtaaggcagg agaaagtaca gatgccctca gcttgcctca
     9601 tgtcaaactg aaataagttc ttcattttct ttccaggcat agcagttctt tagccataca
     9661 tatcattgca tgaactattt cgaaagccct tctaaaaagt tgaaattgca agaatcggga
     9721 agaacatgaa aggcagttta taagcccgtt accttttaat tgcatgaaaa tgcatgttta
     9781 gggatggcta aaattccaag gtgcatcgac attaacccac tcatttagta atgtaccttg
     9841 agttaaaaag cctgagaaac caaacacagc taatgctatg gggtgtatga atatgtcaag
     9901 tttaggtcat ttagaagatt tgacactgta ttttgaaatt atgggagtaa acaccttcaa
     9961 atttcaggca tttctgcttt gtgactaaat acaaactaca ttttcaagat taggccataa
    10021 tgtatattta aacacaatgg ctatcaacag ctgctaataa ggtatcaact aaagcagaat
    10081 tggggaataa tagaaatggc tgcttatttc aagatatatt tgccaaccca ttcctattca
    10141 gtcattttat tattaatgta atttgaatgt caatttgtgt gcttttggtg atttagcgcc
    10201 cgggatccgc ccctctccct cccccc
//
