LOCUS       sequence-404020-       10235 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Heterologous expression in mammalian cell lines or Xenopus oocyte 
            (T7 RNA pol).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10235)
  AUTHORS   Wei AD, Wakenight P, Zwingman TA, Bard AM, Sahai N, Willemsen MH, 
            Schelhaas HJ, Stegmann APA, Verhoeven JS, de Man SA, Wessels MW, 
            Kleefstra T, Shinde DN, Helbig KL, Basinger A, Wagner VF, 
            Rodriguez-Buritica D, Bryant E, Millichap JJ, Millen KJ, Dobyns WB, 
            Ramirez JM, Kalume FK
  TITLE     Human KCNQ5 de novo mutations underlie epilepsy and intellectual 
            disability.
  JOURNAL   J Neurophysiol. 2022 Jul 1;128(1):40-61. doi: 10.1152/jn.00509.2021.
            Epub 2022 May 18.
  PUBMED    35583973
REFERENCE   2  (bases 1 to 10235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1152/jn.00509.2021"; journalName: "J Neurophysiol"; date: 
            "2022-07-1- 1"; volume: "128"; issue: "1"; pages: "40-61"
COMMENT     Sequence Label: hKCNQ5(R178X).ires.GFP-pcDNA3
FEATURES             Location/Qualifiers
     source          1..10235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(156..173)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     383..402
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(576..578,579..581,582..1295)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(576..578,579..581,582..1292)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(621..642)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(882..901)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     promoter        complement(1311..1329)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1312..1329)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1349..1366)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1355..1579
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1625..2053
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1712..1731)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1922..1943
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2062..2082)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2067..2396
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2247..2382
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2309..2328
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2463..3257
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2517..2536)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3127..3146
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3431..3552
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3468..3487)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3522..3541
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3601..3617)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3601..3617)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3614..3636)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3625..3641
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3649..3655,3656..3673,3674..3679))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3694..3715
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3832..3849)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4003..4591)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4083..4102)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4762..5553,5554..5622))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5385..5404
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5623..5727)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5802..5821)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5993..6372
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6373..6576
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6527..6547
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6621..6640
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6621..6639
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           complement(6678..6720)
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     misc_feature    join(10224..10235,1..563,564..566,567..575)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
ORIGIN
        1 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
       61 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
      121 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
      181 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
      241 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
      301 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
      361 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
      421 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtaca catgctttac
      481 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
      541 cctttgaaaa acacgatgat aatatggcca caaccatggt gagcaagggc gaggagctgt
      601 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      661 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
      721 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
      781 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
      841 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
      901 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
      961 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     1021 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     1081 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     1141 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     1201 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     1261 ggatcactct cggcatggac gagctgtaca agtaatctag agggccctat tctatagtgt
     1321 cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     1381 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     1441 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     1501 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     1561 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     1621 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     1681 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     1741 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     1801 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     1861 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     1921 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     1981 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     2041 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     2101 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     2161 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     2221 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     2281 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     2341 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     2401 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc
     2461 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     2521 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     2581 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     2641 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     2701 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     2761 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     2821 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     2881 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     2941 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     3001 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     3061 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     3121 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     3181 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     3241 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     3301 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     3361 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     3421 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     3481 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     3541 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     3601 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     3661 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     3721 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     3781 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     3841 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     3901 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     3961 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     4021 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     4081 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     4141 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     4201 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4261 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     4321 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     4381 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     4441 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4501 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4561 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     4621 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     4681 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     4741 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     4801 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     4861 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     4921 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     4981 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     5041 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     5101 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     5161 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     5221 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     5281 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     5341 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     5401 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     5461 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     5521 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     5581 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     5641 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     5701 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
     5761 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
     5821 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
     5881 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
     5941 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga
     6001 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     6061 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     6121 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     6181 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     6241 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     6301 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     6361 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     6421 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     6481 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     6541 aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact
     6601 gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc ttatcgatac
     6661 cgtcgacgct agctctgcca aagttgagcg tttattctga gcttctgcaa aaagaacaag
     6721 aagggtaccg agctcggatc cactagtaac ggccgccagt gtgctggaat tctgcagata
     6781 tccatcacac tggcggccgc tctagatgcc atgccccgcc accacgcggg aggagaggag
     6841 ggcggcgccg ccgggctctg ggtgaagagc ggcgcagcgg cggcggcggc gggcgggggg
     6901 cgcttgggca gcggcatgaa ggatgtggag tcgggccggg gcagggtgct gctgaactcg
     6961 gcagccgcca ggggcgacgg cctgctactg ctgggcaccc gcgcggccac gcttggtggc
     7021 ggcggcggtg gcctgaggga gagccgccgg ggcaagcagg gggcccggat gagcctgctg
     7081 gggaagccgc tctcttacac gagtagccag agctgccggc gcaacgtcaa gtaccggcgg
     7141 gtgcagaact acctgtacaa cgtgctggag agaccccgcg gctgggcgtt catctaccac
     7201 gctttcgttt ttctccttgt ctttggttgc ttgattttgt cagtgttttc taccatccct
     7261 gagcacacaa aattggcctc aagttgcctc ttgatcctgg agttcgtgat gattgtcgtc
     7321 tttggtttgg agttcatcat ttgaatctgg tctgcgggtt gctgttgtcg atatagagga
     7381 tggcaaggaa gactgaggtt tgctcgaaag cccttctgtg ttatagatac cattgttctt
     7441 atcgcttcaa tagcagttgt ttctgcaaaa actcagggta atatttttgc cacgtctgca
     7501 ctcagaagtc tccgtttcct acagatcctc cgcatggtgc gcatggaccg aaggggaggc
     7561 acttggaaat tactgggttc agtggtttat gctcacagca aggaattaat cacagcttgg
     7621 tacataggat ttttggttct tattttttcg tctttccttg tctatctggt ggaaaaggat
     7681 gccaataaag agttttctac atatgcagat gctctctggt ggggcacaat tacattgaca
     7741 actattggct atggagacaa aactccccta acttggctgg gaagattgct ttctgcaggc
     7801 tttgcactcc ttggcatttc tttctttgca cttcctgccg gcattcttgg ctcaggtttt
     7861 gcattaaaag tacaagaaca acaccgccag aaacactttg agaaaagaag gaacccagct
     7921 gccaacctca ttcagtgtgt ttggcgtagt tacgcagctg atgagaaatc tgtttccatt
     7981 gcaacctgga agccacactt gaaggccttg cacacctgca gccctaccaa gaaagaacaa
     8041 ggggaagcat caagcagttg gggacagtgg acattgcgtc agaagctaag ttttaaggag
     8101 cgagtgcgca tggctagccc caggggccag agtattaaga gccgacaagc ctcagtaggt
     8161 gacaggaggt ccccaagcac cgacatcaca gccgagggca gtcccaccaa agtgcagaag
     8221 agctggagct tcaacgaccg aacccgcttc cggccctcgc tgcgcctcaa aagttctcag
     8281 ccaaaaccag tgatagatgc tgacacagcc cttggcactg atgatgtata tgatgaaaaa
     8341 ggatgccagt gtgatgtatc agtggaagac ctcaccccac cacttaaaac tgtcattcga
     8401 gctatcagaa ttatgaaatt tcatgttgca aaacggaagt ttaaggaaac attacgtcca
     8461 tatgatgtaa aagatgtcat tgaacaatat tctgctggtc atctggacat gttgtgtaga
     8521 attaaaagcc ttcaaacacg tgttgatcaa attcttggaa aagggcaaat cacatcagat
     8581 aagaagagcc gagagaaaat aacagcagaa catgagacca cagacgatct cagtatgctc
     8641 ggtcgggtgg tcaaggttga aaaacaggta cagtccatag aatccaagct ggactgccta
     8701 ctagacatct atcaacaggt ccttcggaaa ggctctgcct cagccctcgc tttggcttca
     8761 ttccagatcc caccttttga atgtgaacag acatctgact atcaaagccc tgtggatagc
     8821 aaagatcttt cgggttccgc acaaaacagt ggctgcttat ccagatcaac tagtgccaac
     8881 atctcgagag gcctgcagtt cattctgacg ccaaatgagt tcagtgccca gactttctac
     8941 gcgcttagcc ctactatgca cagtcaagca acacaggtgc caattagtca aagcgatggc
     9001 tcagcagtgg cagccaccaa caccattgca aaccaaataa atacggcacc caagccagca
     9061 gccccaacaa ctttacagat cccacctcct ctcccagcca tcaagcatct gcccaggcca
     9121 gaaactctgc accctaaccc tgcaggctta caggaaagca tttctgacgt caccacctgc
     9181 cttgttgcct ccaaggaaaa tgttcaggtt gcacagtcaa atctcaccaa ggaccgttct
     9241 atgaggaaaa gctttgacat gggaggagaa actctgttgt ctgtctgtcc catggtgccg
     9301 aaggacttgg gcaaatcttt gtctgtgcaa aacctgatca ggtcgaccga ggaactgaat
     9361 atacaacttt cagggagtga gtcaagtggc tccagaggca gccaagattt ttaccccaaa
     9421 tggagggaat ccaaattgtt tataactgat gaagaggtgg gtcccgaaga gacagagaca
     9481 gacacttttg atgccgcacc gcagcctgcc agggaagctg cctttgcatc agactctcta
     9541 aggactggaa ggtcacgatc atctcagagc atttgtaagg caggagaaag tacagatgcc
     9601 ctcagcttgc ctcatgtcaa actgaaataa gttcttcatt ttctttccag gcatagcagt
     9661 tctttagcca tacatatcat tgcatgaact atttcgaaag cccttctaaa aagttgaaat
     9721 tgcaagaatc gggaagaaca tgaaaggcag tttataagcc cgttaccttt taattgcatg
     9781 aaaatgcatg tttagggatg gctaaaattc caaggtgcat cgacattaac ccactcattt
     9841 agtaatgtac cttgagttaa aaagcctgag aaaccaaaca cagctaatgc tatggggtgt
     9901 atgaatatgt caagtttagg tcatttagaa gatttgacac tgtattttga aattatggga
     9961 gtaaacacct tcaaatttca ggcatttctg ctttgtgact aaatacaaac tacattttca
    10021 agattaggcc ataatgtata tttaaacaca atggctatca acagctgcta ataaggtatc
    10081 aactaaagca gaattgggga ataatagaaa tggctgctta tttcaagata tatttgccaa
    10141 cccattccta ttcagtcatt ttattattaa tgtaatttga atgtcaattt gtgtgctttt
    10201 ggtgatttag cgcccgggat ccgcccctct ccctc
//
