LOCUS       sequence-404028-       10235 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Heterologous expression in mammalian cell lines or Xenopus oocyte 
            (T7 RNA pol).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10235)
  AUTHORS   Wei AD, Wakenight P, Zwingman TA, Bard AM, Sahai N, Willemsen MH, 
            Schelhaas HJ, Stegmann APA, Verhoeven JS, de Man SA, Wessels MW, 
            Kleefstra T, Shinde DN, Helbig KL, Basinger A, Wagner VF, 
            Rodriguez-Buritica D, Bryant E, Millichap JJ, Millen KJ, Dobyns WB, 
            Ramirez JM, Kalume FK
  TITLE     Human KCNQ5 de novo mutations underlie epilepsy and intellectual 
            disability.
  JOURNAL   J Neurophysiol. 2022 Jul 1;128(1):40-61. doi: 10.1152/jn.00509.2021.
            Epub 2022 May 18.
  PUBMED    35583973
REFERENCE   2  (bases 1 to 10235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1152/jn.00509.2021"; journalName: "J Neurophysiol"; date: 
            "2022-07-1- 1"; volume: "128"; issue: "1"; pages: "40-61"
COMMENT     Sequence Label: hKCNQ5(R443X).ires.GFP-pcDNA3
FEATURES             Location/Qualifiers
     source          1..10235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(152..169)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     379..398
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(572..574,575..577,578..1291)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(572..574,575..577,578..1288)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(617..638)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(878..897)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     promoter        complement(1307..1325)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1308..1325)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1345..1362)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1351..1575
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1621..2049
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1708..1727)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1918..1939
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2058..2078)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2063..2392
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2243..2378
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2305..2324
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2459..3253
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2513..2532)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3123..3142
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3427..3548
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3464..3483)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3518..3537
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3597..3613)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3597..3613)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3610..3632)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3621..3637
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3645..3651,3652..3669,3670..3675))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3690..3711
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3828..3845)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3999..4587)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4079..4098)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4758..5549,5550..5618))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5381..5400
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5619..5723)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5798..5817)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5989..6368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6369..6572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6523..6543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6617..6636
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6617..6635
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           complement(6674..6716)
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     misc_feature    join(10220..10235,1..559,560..562,563..571)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
ORIGIN
        1 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
       61 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
      121 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
      181 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
      241 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
      301 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
      361 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
      421 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtacacatg ctttacatgt
      481 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
      541 tgaaaaacac gatgataata tggccacaac catggtgagc aagggcgagg agctgttcac
      601 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      661 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      721 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
      781 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      841 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
      901 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
      961 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     1021 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     1081 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     1141 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     1201 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     1261 cactctcggc atggacgagc tgtacaagta atctagaggg ccctattcta tagtgtcacc
     1321 taaatgctag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     1381 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     1441 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     1501 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     1561 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     1621 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     1681 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     1741 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     1801 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     1861 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     1921 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     1981 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     2041 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     2101 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     2161 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     2221 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     2281 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     2341 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     2401 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat
     2461 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     2521 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     2581 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     2641 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     2701 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     2761 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     2821 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     2881 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     2941 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     3001 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     3061 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     3121 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     3181 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     3241 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     3301 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     3361 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     3421 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     3481 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     3541 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     3601 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     3661 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     3721 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     3781 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     3841 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     3901 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     3961 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     4021 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     4081 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     4141 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     4201 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     4261 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     4321 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     4381 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     4441 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     4501 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     4561 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     4621 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     4681 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     4741 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     4801 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     4861 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     4921 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     4981 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     5041 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     5101 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     5161 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     5221 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     5281 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     5341 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     5401 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     5461 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     5521 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     5581 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     5641 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     5701 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
     5761 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat
     5821 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
     5881 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
     5941 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat
     6001 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     6061 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     6121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     6181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     6241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     6301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     6361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     6421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     6481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     6541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactagagaa cccactgctt
     6601 actggcttat cgaaattaat acgactcact atagggagac ccaagcttat cgataccgtc
     6661 gacgctagct ctgccaaagt tgagcgttta ttctgagctt ctgcaaaaag aacaagaagg
     6721 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattctg cagatatcca
     6781 tcacactggc ggccgctcta gatgccatgc cccgccacca cgcgggagga gaggagggcg
     6841 gcgccgccgg gctctgggtg aagagcggcg cagcggcggc ggcggcgggc ggggggcgct
     6901 tgggcagcgg catgaaggat gtggagtcgg gccggggcag ggtgctgctg aactcggcag
     6961 ccgccagggg cgacggcctg ctactgctgg gcacccgcgc ggccacgctt ggtggcggcg
     7021 gcggtggcct gagggagagc cgccggggca agcagggggc ccggatgagc ctgctgggga
     7081 agccgctctc ttacacgagt agccagagct gccggcgcaa cgtcaagtac cggcgggtgc
     7141 agaactacct gtacaacgtg ctggagagac cccgcggctg ggcgttcatc taccacgctt
     7201 tcgtttttct ccttgtcttt ggttgcttga ttttgtcagt gttttctacc atccctgagc
     7261 acacaaaatt ggcctcaagt tgcctcttga tcctggagtt cgtgatgatt gtcgtctttg
     7321 gtttggagtt catcattcga atctggtctg cgggttgctg ttgtcgatat agaggatggc
     7381 aaggaagact gaggtttgct cgaaagccct tctgtgttat agataccatt gttcttatcg
     7441 cttcaatagc agttgtttct gcaaaaactc agggtaatat ttttgccacg tctgcactca
     7501 gaagtctccg tttcctacag atcctccgca tggtgcgcat ggaccgaagg ggaggcactt
     7561 ggaaattact gggttcagtg gtttatgctc acagcaagga attaatcaca gcttggtaca
     7621 taggattttt ggttcttatt ttttcgtctt tccttgtcta tctggtggaa aaggatgcca
     7681 ataaagagtt ttctacatat gcagatgctc tctggtgggg cacaattaca ttgacaacta
     7741 ttggctatgg agacaaaact cccctaactt ggctgggaag attgctttct gcaggctttg
     7801 cactccttgg catttctttc tttgcacttc ctgccggcat tcttggctca ggttttgcat
     7861 taaaagtaca agaacaacac cgccagaaac actttgagaa aagaaggaac ccagctgcca
     7921 acctcattca gtgtgtttgg cgtagttacg cagctgatga gaaatctgtt tccattgcaa
     7981 cctggaagcc acacttgaag gccttgcaca cctgcagccc taccaagaaa gaacaagggg
     8041 aagcatcaag cagttgggga cagtggacat tgcgtcagaa gctaagtttt aaggagtgag
     8101 tgcgcatggc tagccccagg ggccagagta ttaagagccg acaagcctca gtaggtgaca
     8161 ggaggtcccc aagcaccgac atcacagccg agggcagtcc caccaaagtg cagaagagct
     8221 ggagcttcaa cgaccgaacc cgcttccggc cctcgctgcg cctcaaaagt tctcagccaa
     8281 aaccagtgat agatgctgac acagcccttg gcactgatga tgtatatgat gaaaaaggat
     8341 gccagtgtga tgtatcagtg gaagacctca ccccaccact taaaactgtc attcgagcta
     8401 tcagaattat gaaatttcat gttgcaaaac ggaagtttaa ggaaacatta cgtccatatg
     8461 atgtaaaaga tgtcattgaa caatattctg ctggtcatct ggacatgttg tgtagaatta
     8521 aaagccttca aacacgtgtt gatcaaattc ttggaaaagg gcaaatcaca tcagataaga
     8581 agagccgaga gaaaataaca gcagaacatg agaccacaga cgatctcagt atgctcggtc
     8641 gggtggtcaa ggttgaaaaa caggtacagt ccatagaatc caagctggac tgcctactag
     8701 acatctatca acaggtcctt cggaaaggct ctgcctcagc cctcgctttg gcttcattcc
     8761 agatcccacc ttttgaatgt gaacagacat ctgactatca aagccctgtg gatagcaaag
     8821 atctttcggg ttccgcacaa aacagtggct gcttatccag atcaactagt gccaacatct
     8881 cgagaggcct gcagttcatt ctgacgccaa atgagttcag tgcccagact ttctacgcgc
     8941 ttagccctac tatgcacagt caagcaacac aggtgccaat tagtcaaagc gatggctcag
     9001 cagtggcagc caccaacacc attgcaaacc aaataaatac ggcacccaag ccagcagccc
     9061 caacaacttt acagatccca cctcctctcc cagccatcaa gcatctgccc aggccagaaa
     9121 ctctgcaccc taaccctgca ggcttacagg aaagcatttc tgacgtcacc acctgccttg
     9181 ttgcctccaa ggaaaatgtt caggttgcac agtcaaatct caccaaggac cgttctatga
     9241 ggaaaagctt tgacatggga ggagaaactc tgttgtctgt ctgtcccatg gtgccgaagg
     9301 acttgggcaa atctttgtct gtgcaaaacc tgatcaggtc gaccgaggaa ctgaatatac
     9361 aactttcagg gagtgagtca agtggctcca gaggcagcca agatttttac cccaaatgga
     9421 gggaatccaa attgtttata actgatgaag aggtgggtcc cgaagagaca gagacagaca
     9481 cttttgatgc cgcaccgcag cctgccaggg aagctgcctt tgcatcagac tctctaagga
     9541 ctggaaggtc acgatcatct cagagcattt gtaaggcagg agaaagtaca gatgccctca
     9601 gcttgcctca tgtcaaactg aaataagttc ttcattttct ttccaggcat agcagttctt
     9661 tagccataca tatcattgca tgaactattt cgaaagccct tctaaaaagt tgaaattgca
     9721 agaatcggga agaacatgaa aggcagttta taagcccgtt accttttaat tgcatgaaaa
     9781 tgcatgttta gggatggcta aaattccaag gtgcatcgac attaacccac tcatttagta
     9841 atgtaccttg agttaaaaag cctgagaaac caaacacagc taatgctatg gggtgtatga
     9901 atatgtcaag tttaggtcat ttagaagatt tgacactgta ttttgaaatt atgggagtaa
     9961 acaccttcaa atttcaggca tttctgcttt gtgactaaat acaaactaca ttttcaagat
    10021 taggccataa tgtatattta aacacaatgg ctatcaacag ctgctaataa ggtatcaact
    10081 aaagcagaat tggggaataa tagaaatggc tgcttatttc aagatatatt tgccaaccca
    10141 ttcctattca gtcattttat tattaatgta atttgaatgt caatttgtgt gcttttggtg
    10201 atttagcgcc cgggatccgc ccctctccct ccccc
//
