LOCUS       sequence-404031-        5370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Genetically encoded fluorescent indicator for measuring NAD/NADH 
            ratio..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Bilan DS, Matlashov ME, Gorokhovatsky AY, Schultz C, Enikolopov G, 
            Belousov VV
  TITLE     Genetically encoded fluorescent indicator for imaging NAD/NADH ratio
            changes in different cellular compartments.
  JOURNAL   Biochim Biophys Acta. 2013 Nov 25. pii: S0304-4165(13)00519-9. doi: 
            10.1016/j.bbagen.2013.11.018.
  PUBMED    24286672
REFERENCE   2  (bases 1 to 5370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochim 
            Biophys Acta. 2013 Nov 25. pii: S0304-4165(13)00519-9. doi: 
            10.1016/j.bbagen.2013.11.018."
COMMENT     Sequence Label: pC1-REX-YFP
FEATURES             Location/Qualifiers
     source          1..5370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             112..906
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             112..906
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(166..185)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     776..795
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1094..1113)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1138..1185
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1514..2102
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2003..2022
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2277..2580
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2581..2784
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2735..2755
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3283..3304
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3413..3434)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3674..3693)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    4374..4495
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4411..4430)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4465..4484
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4502..4957)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4639..4660)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4851..4870
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4984..5088
                     /gene="bla"
                     /label=AmpR promoter
     promoter        join(5090..5370,1..77)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5090..5281
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5113..5133)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      join(5298..5370,1..63)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
       61 gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa
      121 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
      181 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
      241 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag
      301 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
      361 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
      421 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
      481 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
      541 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
      601 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat
      661 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
      721 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
      781 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
      841 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
      901 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
      961 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     1021 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta
     1081 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg
     1141 caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc
     1201 ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc
     1261 gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag
     1321 ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg
     1381 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     1441 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     1501 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     1561 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     1621 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     1681 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     1741 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     1801 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     1861 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     1921 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     1981 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2041 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2101 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2161 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcattagt
     2221 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     2281 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     2341 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     2401 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     2461 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     2521 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     2581 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     2641 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     2701 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     2761 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgccaccat
     2821 gaaggtcccc gaagcggcca tcagccgcct gatcacctac ctccgcatcc tggaggagct
     2881 ggaggcccag ggggttcacc ggacgagctc cgagcagctt ggggagctcg cccaggtcac
     2941 ggccttccag gtgcggaagg acctttccta cttcggctcc tacgggacgc gcggggtggg
     3001 gtacaccgtc cccgtcctga agcgggagct ccgccacatc ctggggctca accgctccgc
     3061 cggctacaac agcgacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggc
     3121 caacttcaag atccgccaca acgtcgagga cggcagcgtg cagctcgccg accactacca
     3181 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagctt
     3241 ccagtccgtc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     3301 cgtgaccgcc gccgggatca ctcccggcat ggacgagctg aacaacgtgg atggcggtag
     3361 cggtggcacc ggcagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     3421 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     3481 cacctacggc aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg
     3541 gcccaccctc gtgaccaccc tcggctacgg cctgaagtgc ttcgcccgct accccgacca
     3601 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     3661 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     3721 caccctggtg aaccgcatcg agctgaaggg catcggcttc aaggaggacg gcaacatcct
     3781 ggggcacaag ctggagtaca acggcaccaa gtggggcctg tgcatcgtgg gcatgggccg
     3841 gctcgggagc gccctggccg actacccggg gttcggggag agctttgagc tcaggggctt
     3901 ctttgacgtg gacccggaga aggtgggccg cccggtgcgg ggcggggtca ttgagcacgt
     3961 ggacctcctg ccccagcggg ttcccggccg cattgagatc gccctcctca ccgtgccccg
     4021 ggaagcggcc cagaaggcgg cggacctgtt ggtggcggcg gggatcaagg ggatcctgaa
     4081 cttcgccccg gtggtcctcg aggtgcccaa ggaggtggcg gtggagaacg tggacttctt
     4141 ggcggggctc acgcggcttt ctttcgctat cctgaacccc aagtggcggg aagagatgat
     4201 ggggtgaagc ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc accggatcta
     4261 gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     4321 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     4381 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     4441 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg
     4501 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     4561 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     4621 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     4681 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     4741 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     4801 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     4861 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     4921 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat
     4981 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     5041 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa
     5101 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     5161 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     5221 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     5281 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     5341 atggctgact aatttttttt atttatgcag
//
