LOCUS       sequence-404033-       11488 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus vector expressing vinculin with FRET conformational 
            sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11488)
  AUTHORS   Tao A, LaCroix AS, Shoyer TC, Venkatraman V, Xu KL, Feiger B, 
            Hoffman BD
  TITLE     Identifying constitutive and context-specific 
            molecular-tension-sensitive protein recruitment within focal 
            adhesions.
  JOURNAL   Dev Cell. 2023 Mar 10:S1534-5807(23)00074-6. doi: 
            10.1016/j.devcel.2023.02.015.
  PUBMED    36924770
REFERENCE   2  (bases 1 to 11488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2023 Mar 10:S1534-5807(23)00074-6. doi: 
            10.1016/j.devcel.2023.02.015."
COMMENT     Sequence Label: pRRL-VinculinCS
FEATURES             Location/Qualifiers
     source          1..11488
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      54..642
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     543..562
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     796..813
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    930..951
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(966..971,972..989,990..996)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1004..1020
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1009..1031
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1028..1044
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1028..1044
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1063..1083
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1065..1083
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1111..1337
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1338..1518
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1565..1690
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2183..2416
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2601..2645
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2912..3028
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3088..3467
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3468..3671
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3622..3642
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        3716..3734
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6428..7135
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             join(7700..7702,7703..7705,7706..8419)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7745..7766)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8006..8025)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8353..8374
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8438..9026
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8491..8511)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8909..8920)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9117..9350
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9422..9543
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9459..9478)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9513..9532
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9583..9718
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(9739..9757)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9767..9784)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9767..9783)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9776..9798)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9925..10380
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10012..10031)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10222..10243
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10406..10510
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10511..10579,10580..11371)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10729..10748)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
       61 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
      121 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
      181 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
      241 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
      301 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
      361 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
      421 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
      481 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
      541 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
      601 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
      661 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
      721 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
      781 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
      841 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
      901 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
      961 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     1021 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     1081 agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt
     1141 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat
     1201 gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc
     1261 tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc
     1321 tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     1381 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     1441 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     1501 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     1561 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     1621 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     1681 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     1741 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     1801 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     1861 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     1921 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     1981 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
     2041 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     2101 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     2161 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     2221 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     2281 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     2341 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     2401 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     2461 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     2521 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     2581 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     2641 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     2701 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
     2761 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
     2821 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
     2881 tagtgaacgg atctcgacgg tatcggttaa cttttaaaag aaaagggggg attggggggt
     2941 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     3001 aaaaacaaat tacaaaattc aaaattttat cgatggatcc cccgggctcg agttcgaaga
     3061 tcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
     3121 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     3181 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     3241 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     3301 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     3361 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     3421 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     3481 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     3541 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     3601 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     3661 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata
     3721 cgactcacta tagggagacc caagctggct agcgtttaaa cttaagcttg ccatgcccgt
     3781 cttccacacg cgcaccatcg agagcatctt ggagcccgtg gctcagcaga tctcccacct
     3841 ggtcatcatg cacgaggagg gggaggtaga cggcaaggcc atcccggacc tcaccgcccc
     3901 cgtgtcggcc gtgcaggccg ctgtcagcaa cctggtgcgg gttggaaaag aaactgtgca
     3961 gacaacagaa gaccagatct tgaaaaggga tatgccacca gcattcatca aagtagagaa
     4021 tgcctgcacc aagctcgttc gagcagccca gatgctgcaa gcagatcctt attcagtacc
     4081 agctcgtgac tacctaattg atggatcaag aggcatcctt tctggaacat cagacttact
     4141 tctgacattt gatgaagcgg aggtccgtaa aatcatccgt gtctgcaaag gaatattgga
     4201 atatctgact gtggcagaag tagtagagac tatggaggat ttggtgacat atacaaagaa
     4261 tctagggcca ggaatgacaa agatggcgaa aatgatcgat gagagacaac aggaattaac
     4321 tcatcaggaa catagggtta tgctggtgaa ctccatgaat actgtgaagg agctattgcc
     4381 tgtacttatt tcagctatga agatctttgt aaccacaaaa aacactaaaa gccagggaat
     4441 agaagaggcc ttgaaaaatc gcaatttcac agtagagaaa atgagtgctg agataaatga
     4501 aataatccgt gtattacaac tcacttcctg ggatgaagat gcctgggcca gcaaggacac
     4561 tgaagccatg aaaagagctt tagccctaat agattcaaag atgaaccagg caaaaggctg
     4621 gctgagagat ccaaacgcac ctccagggga tgctggtgag caagcaataa ggcagatcct
     4681 tgatgaagct ggaaaagcag gagaattgtg tgcaggcaaa gaacgcaggg agattctggg
     4741 aacatgcaaa actttgggcc aaatgactga tcaacttgct gatctccgag ctagaggaca
     4801 gggtgctaca ccgatggcaa tgcagaaggc acagcaggtg tcacaaggcc tggatttgct
     4861 cactgcaaaa gtggagaatg cagcccggaa attggaggcc atgacaaact ctaagcaggc
     4921 tattgcaaag aagattgatg ctgctcagaa ctggcttgcg gatcccaacg gcggaagtga
     4981 aggagaagaa cacattcgag gaattatgtc tgaagcaagg aaagttgcag aattgtgtga
     5041 ggagcctaaa gaaagagatg atatccttcg ctccttgggg gaaatctctg ctctgacagc
     5101 taagctgtca gatctgcgac gacatgggaa aggcgactct cctgaggccc gtgcattggc
     5161 caagcaaata gctacatcac ttcagaactt acagtccaaa acaaacagag ctgtagcaaa
     5221 tactaggcca gttaaagctg ctgtccattt ggagggcaag attgagcaag cccagaggtg
     5281 gatagataat cctacagttg atgatcgggg agtaggccag gcagcaattc ggggtttggt
     5341 tgcagaaggt cgtcgtctag ccaatgtcat gatgggacct tatcgtcagg acctgcttgc
     5401 caaatgtgac cgtgtagacc agctggctgc tcagcttgct gaccttgcag caagagggga
     5461 aggagagtct cctcaggcta gggcaattgc tgctcagctt caggactccc tgaaggatct
     5521 caaagcacgg atgcaagaag caatgaccca ggaggtgtct gatgttttca gtgacactac
     5581 aacccctatt aagttgttag cagtagcagc cactgctcct tctgatactc ccaatagaga
     5641 agaggtgttt gaagaaagag cagcaaattt tgaaaaccat gctgctagac tgggagcaac
     5701 agcagaaaaa gcagctgcag ttggaactgc taataaaact actgtggaag gcattcaggc
     5761 aacagtcaaa tctgcaaggg agcttacacc acaggtagta tcggctgctc gaatcctcct
     5821 gagaaatcct ggaaatcaag ctgcttatga gcattttgag acaatgaaaa accaatggat
     5881 tgataatgta gaaaagatga cagggctggt ggatgaggcc atcgatacca agtctctgtt
     5941 ggatgcatca gaagaggcta ttaagaagga tcttgataaa tgtaaagttg caatggccaa
     6001 tatgcaacct cagatgctgg tagctggagc caccagcatt gctagacgag cgaaccgcat
     6061 cctgcttgtg gcaaaacggg aggttgaaaa ttcagaagac cctaaattca gggaggctgt
     6121 taaagcagct tctgatgagc tgagcaaaac catatcaccg atggtaatgg atgctaaagc
     6181 tgtagcagga aatatctctg atcctggttt gcagaagagt ttcttggatt ctggatacag
     6241 gattctggga gctgtggcca aagtcagaga agcctttcag cctcaggagc cagactttcc
     6301 ccctcctcct cctgacctag agcatctcca tctgactgat gagcttgctc ctccaaaacc
     6361 accacttcca gaaggtgagg ttcccccacc cagaccacca ccccctgaag aaaaagatga
     6421 ggtcgagatg gtgagcaagg gcgaggagac cacaatgggc gtaatcaagc ccgacatgaa
     6481 gatcaagctg aagatggagg gcaacgtgaa tggccacgcc ttcgtgatcg agggcgaggg
     6541 cgagggcaag ccctacgacg gcaccaacac catcaacctg gaggtgaagg agggagcccc
     6601 cctgcccttc tcctacgaca ttctgaccac cgcgttcgcc tacggcaaca gggccttcac
     6661 caagtacccc gacgacatcc ccaactactt caagcagtcc ttccccgagg gctactcttg
     6721 ggagcgcacc atgaccttcg aggacaaggg catcgtgaag gtgaagtccg acatctccat
     6781 ggaggaggac tccttcatct acgagataca cctcaagggc gagaacttcc cccccaacgg
     6841 ccccgtgatg cagaagaaga ccaccggctg ggacgcctcc accgagagga tgtacgtgcg
     6901 cgacggcgtg ctgaagggcg acgtcaagca caagctgctg ctggagggcg gcggccacca
     6961 ccgcgttgac ttcaagacca tctacagggc caagaaggcg gtgaagctgc ccgactatca
     7021 ctttgtggac caccgcatcg agatcctgaa ccacgacaag gactacaaca aggtgaccgt
     7081 ttacgagagc gccgtggccc gcaactccac cgacggcatg gacgagctgt acaaggcggc
     7141 cgcggagttc ccagagcaga aagcaggaga agctattaat cagcccatga tgatggctgc
     7201 taggcagttg catgacgagg ctcggaaatg gtctagcaag ggtaacgaca tcattgctgc
     7261 tgctaaacga atggcgctgc tcatggctga gatgtcacgc ctggtgcgag gaggcagcgg
     7321 aaacaagcgt gccctcatcc agtgtgcaaa agatattgct aaggcatcgg atgaagtcac
     7381 tcgattggcc aaagaggtgg ccaagcaatg tactgataaa cgcattagaa caaacctctt
     7441 acaggtctgt gagcgaatcc caaccatcag cacgcagctc aaaattcttt ccacagtgaa
     7501 agctaccatg ctgggcagga ctaacatcag cgatgaagaa tcagaacagg caactgagat
     7561 gttggttcat aacgcccaga acctcatgca gtctgtgaag gaaactgtga gagaagctga
     7621 agcagcatcc attaagataa gaacagatgc cggattcact ctgcgctggg tcagaaagac
     7681 cccatggtat caggaattca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     7741 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     7801 cgagggcgat gccacctacg gcaagctgac cctgaagctg atctgcacca ccggcaagct
     7861 gcccgtgccc tggcccaccc tcgtgaccac cctgggctac ggcctgcagt gcttcgcccg
     7921 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     7981 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     8041 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     8101 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcac
     8161 cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga
     8221 cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     8281 gctgctgccc gacaaccact acctgagcta ccagtccaaa ctgagcaaag accccaacga
     8341 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     8401 ggacgagctg tacaagtaaa tcgattctag agtcgacaat caacctctgg attacaaaat
     8461 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     8521 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     8581 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     8641 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     8701 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     8761 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     8821 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     8881 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     8941 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     9001 gatctccctt tgggccgcct ccccgcctgg aattaattcg agctcggtac ctttaagacc
     9061 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     9121 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     9181 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     9241 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     9301 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     9361 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     9421 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     9481 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     9541 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     9601 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     9661 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     9721 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     9781 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     9841 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     9901 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     9961 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
    10021 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
    10081 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
    10141 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
    10201 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
    10261 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
    10321 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
    10381 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
    10441 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
    10501 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
    10561 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
    10621 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
    10681 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
    10741 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
    10801 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
    10861 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
    10921 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
    10981 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
    11041 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
    11101 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
    11161 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
    11221 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
    11281 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
    11341 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
    11401 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
    11461 taatctcatg accaaaatcc cttaacgt
//
