LOCUS       sequence-404034-       12341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-One piggyBac transposon Destination vector for dox-inducible 
            expression of Gateway cloned elements (inducible mCherry and 
            constitutive rtTA and puromycin resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12341)
  AUTHORS   Kim SI, Oceguera-Yanez F, Sakurai C, Nakagawa M, Yamanaka S, Woltjen
            K
  TITLE     Inducible Transgene Expression in Human iPS Cells Using Versatile 
            All-in-One piggyBac Transposons.
  JOURNAL   Methods Mol Biol. 2016;1357:111-31. doi: 10.1007/7651_2015_251.
  PUBMED    26025620
REFERENCE   2  (bases 1 to 12341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2016"; volume: "1357:111-31. doi"; pages: " 
            10.1007/7651_2015_251"
COMMENT     Sequence Label: PB-TAC-ERP2
FEATURES             Location/Qualifiers
     source          1..12341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(28..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     350..369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     621..640
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        621..639
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   706..768
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1140..1158
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1177..1195
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1214..1232
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1251..1269
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1288..1306
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1325..1343
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1362..1380
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1399..1417
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1439..1463
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1543..1667
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1692..1697,1698..1715,1716..1722)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1776..2435
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1842..1861)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2777..3082
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2961..2980
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3123..3247)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    join(3289..3863,3864..3866,3867..3875)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             3876..4586
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4021..4039)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4289..4308
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(4598..4617)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4663..4718
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4717..4736)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     enhancer        5161..5540
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5655..6960
                     /label=rEF-1-alpha promoter
                     /note="strong constitutive promoter for rat elongation 
                     factor EF-1-alpha"
     misc_feature    5997..6928
                     /label=rEF-1-alpha intron
                     /note="intron upstream of the start codon of rat 
                     EF-1-alpha"
     CDS             join(6977..7594,7595..7600,7601..7636,7637..7639,7640..7675,
                     7676..7678,7679..7714,7715..7723)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    join(7750..8324,8325..8327,8328..8336)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             8337..8936
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8337..8356)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8833..8853
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    9022..9594
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
     repeat_region   9968..10002
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(10151..10169)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10190..10206)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10190..10206)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10203..10225)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10214..10230
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10238..10244,10245..10262,10263..10268))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10283..10304
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10421..10438)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10592..11180)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10672..10691)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11351..12142,12143..12211))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11974..11993
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12212..12316)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgaattg taatacgact cactataggg cgaattggag ctcggtattc
      661 acgacagcag gctgaataat aaaaaaatta gaaactatta tttaacccta gaaagataat
      721 catattgtga cgtacgttaa agataatcat gcgtaaaatt gacgcatgtg ttttatcggt
      781 ctgtatatcg aggtttattt attaatttga atagatatta agttttatta tatttacact
      841 tacatactaa taataaattc aacaaacaat ttatttatgt ttatttattt attaaaaaaa
      901 aacaaaaact caaaatttct tctataaagt aacaaaactt ttaaacattc tctcttttac
      961 aaaaataaac ttattttgta ctttaaaaac agtcatgttg tattataaaa taagtaatta
     1021 gcttaaccta tacataatag aaacaaatta tacttattag tcagtcagaa acaactttgg
     1081 cacatatcaa tattatgctc tcgttaatta actcgaggga tccaagctgg ccgcacgtct
     1141 ccctatcagt gatagagaag tcgacacgtc tcgagctccc tatcagtgat agagaaggta
     1201 cgtctagaac gtctccctat cagtgataga gaagtcgaca cgtctcgagc tccctatcag
     1261 tgatagagaa ggtacgtcta gaacgtctcc ctatcagtga tagagaagtc gacacgtctc
     1321 gagctcccta tcagtgatag agaaggtacg tctagaacgt ctccctatca gtgatagaga
     1381 agtcgacacg tctcgagctc cctatcagtg atagagaagg taccccctat ataagcagag
     1441 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     1501 gaagacaccg ggaccgatcc agcctccgcg gtggcggcca tcacaagttt gtacaaaaaa
     1561 gctgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa
     1621 aaacagacta cataatactg taaaacacaa catatccagt cactatggcg gccgcattag
     1681 gcaccccagg ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg
     1741 atccgtcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata
     1801 ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg
     1861 ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa
     1921 agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg
     1981 ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc
     2041 acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat
     2101 accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg
     2161 aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc
     2221 cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc
     2281 ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga
     2341 ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac
     2401 aacagtactg cgatgagtgg cagggcgggg cgtaaacgcg tggatccggc ttactaaaag
     2461 ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata
     2521 tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta cagtgacagt
     2581 tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta
     2641 agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat
     2701 caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag
     2761 aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg
     2821 tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc
     2881 cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca
     2941 tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt
     3001 tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa
     3061 cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg
     3121 accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa
     3181 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca
     3241 aagtggtgat ctagctaaaa cgcggcctcg aatcgaattc cgcccccccc cctctccctc
     3301 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     3361 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     3421 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     3481 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     3541 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     3601 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     3661 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     3721 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
     3781 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
     3841 cctttgaaaa acacgatgat aatatggcca caaccatggt gagcaagggc gaggaggata
     3901 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     3961 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     4021 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     4081 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     4141 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     4201 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     4261 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     4321 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     4381 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     4441 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     4501 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     4561 gcggcatgga cgagctgtac aagtaaagcg gccgcgatct ttttccctct gccaaaaatt
     4621 atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt
     4681 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg
     4741 caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata
     4801 tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc
     4861 ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat
     4921 tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag
     4981 ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga
     5041 gatccctcga cctgcaggaa ttcgattact taagcctagg atccagatct gtacattcga
     5101 atcgatgaat tcgttaacca tggaagcttc tgcagcggcc gcactagcgg tggcggcctc
     5161 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     5221 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     5281 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     5341 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     5401 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     5461 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     5521 tagtcatcgc tattaccatg atgatgcggt tttggcagta catcaatggg cgtggatagc
     5581 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     5641 gactagtgga gccgagagta attcatacaa aaggagggat cgccttcgca aggggagagc
     5701 ccagggaccg tccctaaatt ctcacagacc caaatccctg tagccgcccc acgacagcgc
     5761 gaggagcatg cgcccagggc tgagcgcggg tagatcagag cacacaagct cacagtcccc
     5821 ggcggtgggg ggaggggcgc gctgagcggg ggccagggag ctggcgcggg gcaaactggg
     5881 aaagtggtgt cgtgtgctgg ctccgccctc ttcccgaggg tgggggagaa cggtatataa
     5941 gtgcggtagt cgccttggac gttctttttc gcaacgggtt tgccgtcaga acgcaggtga
     6001 gtggcgggtg tggcttccgc gggccccgga gctggagccc tgctctgagc gggccgggct
     6061 gatatgcgag tgtcgtccgc agggtttagc tgtgagcatt cccacttcga gtggcgggcg
     6121 gtgcgggggt gagagtgcga ggcctagcgg caaccccgta gcctcgcctc gtgtccggct
     6181 tgaggcctag cgtggtgtcc gccgccgcgt gccactccgg ccgcactatg cgttttttgt
     6241 ccttgctgcc ctcgattgcc ttccagcagc atgggctaac aaagggaggg tgtggggctc
     6301 actcttaagg agcccatgaa gcttacgttg gataggaatg gaagggcagg aggggcgact
     6361 ggggcccgcc cgccttcgga gcacatgtcc gacgccacct ggatggggcg aggcctgtgg
     6421 ctttccgaag caatcgggcg tgagtttagc ctacctgggc catgtggccc tagcactggg
     6481 cacggtctgg cctggcggtg ccgcgttccc ttgcctccca acaagggtga ggccgtcccg
     6541 cccggcacca gttgcttgcg cggaaagatg gccgctcccg gggccctgtt gcaaggagct
     6601 caaaatggag gacgcggcag cccggtggag cgggcgggtg agtcacccac acaaaggaag
     6661 agggccttgc ccctcgccgg ccgctgcttc ctgtgacccc gtggtctatc ggccgcatag
     6721 tcacctcggg cttctcttga gcaccgctcg tcgcggcggg gggaggggat ctaatggcgt
     6781 tggagtttgt tcacatttgg tgggtggaga ctagtcaggc cagcctggcg ctggaagtca
     6841 ttcttggaat ttgccccttt gagtttggag cgaggctaat tctcaagcct cttagcggtt
     6901 caaaggtatt ttctaaaccc gtttccaggt gttgtgaaag ccaccgctaa ttcaaagcaa
     6961 tccggagtaa ttcaccatgt ctagactgga caagagcaaa gtcataaacg gcgctctgga
     7021 attactcaat ggagtcggta tcgaaggcct gacgacaagg aaactcgctc aaaagctggg
     7081 agttgagcag cctaccctgt actggcacgt gaagaacaag cgggccctgc tcgatgccct
     7141 gccaatcgag atgctggaca ggcatcatac ccacttctgc cccctggaag gcgagtcatg
     7201 gcaagacttt ctgcggaaca acgccaagtc attccgctgt gctctcctct cacatcgcga
     7261 cggggctaaa gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa
     7321 tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag aacgcactgt acgctctgtc
     7381 cgccgtgggc cactttacac tgggctgcgt attggaggaa caggagcatc aagtagcaaa
     7441 agaggaaaga gagacaccta ccaccgattc tatgccccca cttctgagac aagcaattga
     7501 gctgttcgac cggcagggag ccgaacctgc cttccttttc ggcctggaac taatcatatg
     7561 tggcctggag aaacagctaa agtgcgaaag cggcgggccg gccgacgccc ttgacgattt
     7621 tgacttagac atgctcccag ccgatgccct tgacgacttt gaccttgata tgctgcctgc
     7681 tgacgctctt gacgattttg accttgacat gctccccggg taactaagta aggatcattc
     7741 cgcccccccc ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata
     7801 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     7861 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     7921 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     7981 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     8041 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     8101 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     8161 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     8221 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     8281 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatga
     8341 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     8401 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     8461 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     8521 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     8581 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     8641 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     8701 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     8761 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     8821 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     8881 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc
     8941 cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tcgaaatgac
     9001 cgaccaagcg aattcgctag gattatccct aatacctgcc accccactct taatcagtgg
     9061 tggaagaacg gtctcagaac tgtttgtttc aattggccat ttaagtttag tagtaaaaga
     9121 ctggttaatg ataacaatgc atcgtaaaac cttcagaagg aaaggagaat gttttgtgga
     9181 ccactttggt tttctttttt gcgtgtggca gttttaagtt attagttttt aaaatcagta
     9241 ctttttaatg gaaacaactt gaccaaaaat ttgtcacaga attttgagac ccattaaaaa
     9301 agttaaatga gaaacctgtg tgttcctttg gtcaacaccg agacatttag gtgaaagaca
     9361 tctaattctg gttttacgaa tctggaaact tcttgaaaat gtaattcttg agttaacact
     9421 tctgggtgga gaatagggtt gttttccccc cacataattg gaaggggaag gaatatcatt
     9481 taaagctatg ggagggttgc tttgattaca acactggaga gaaatgcagc atgttgctga
     9541 ttgcctgtca ctaaaacagg ccaaaaactg agtccttggg ttgcatagaa agctgccaaa
     9601 cgctagccaa caagctcgtc atcgctttgc agaagagcag agaggatatg ctcatcgtct
     9661 aaagaactac ccattttatt atatattagt cacgatatct ataacaagaa aatatatata
     9721 taataagtta tcacgtaagt agaacatgaa ataacaatat aattatcgta tgagttaaat
     9781 cttaaaagtc acgtaaaaga taatcatgcg tcattttgac tcacgcggtc gttatagttc
     9841 aaaatcagtg acacttaccg cattgacaag cacgcctcac gggagctcca agcggcgact
     9901 gagatgtcct aaatgcacag cgacggattc gcgctattta gaaagagaga gcaatatttc
     9961 aagaatgcat gcgtcaattt tacgcagact atctttctag ggttaaaaaa gatttgcgct
    10021 ttactcgacc taaactttaa acacgtcata gaatcttcgt ttgacaaaaa ccacattgtg
    10081 gccaagctgt gtgacgcgac gcgcgctaaa gaatggcaaa ccaagtcgcg cgaggtacca
    10141 gcttttgttc cctttagtga gggttaattt cgagcttggc gtaatcatgg tcatagctgt
    10201 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
    10261 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
    10321 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
    10381 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
    10441 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
    10501 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
    10561 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
    10621 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
    10681 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
    10741 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
    10801 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
    10861 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
    10921 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
    10981 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
    11041 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
    11101 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
    11161 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    11221 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
    11281 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
    11341 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
    11401 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
    11461 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
    11521 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
    11581 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    11641 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    11701 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    11761 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    11821 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    11881 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    11941 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    12001 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    12061 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    12121 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    12181 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    12241 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    12301 aaataggggt tccgcgcaca tttccccgaa aagtgccacc t
//
