LOCUS       sequence-404041-       15239 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15239)
  AUTHORS   Hunkeler M, Jin CY, Ma MW, Monda JK, Overwijn D, Bennett EJ, Fischer
            ES
  TITLE     Solenoid architecture of HUWE1 contributes to ligase activity and 
            substrate recognition.
  JOURNAL   Mol Cell. 2021 Sep 2;81(17):3468-3480.e7. doi: 
            10.1016/j.molcel.2021.06.032. Epub 2021 Jul 26.
  PUBMED    34314700
REFERENCE   2  (bases 1 to 15239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.06.032"; journalName: "Mol Cell"; date: 
            "2021-09-2- 2"; volume: "81"; issue: "17"; pages: "3468-3480.e7"
COMMENT     Sequence Label: pDONR221-HUWE1_delta3896-3951
FEATURES             Location/Qualifiers
     source          1..15239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(7811..7910)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(7929..7952)
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     terminator      complement(7960..7987)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(8079..8165)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(8330..8918)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8410..8429)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9008..9817)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9731..9750
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9809..9828
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    9940..10039
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ctgctgctca gctgaaggat gtcagcagca gctgagggac caagcaacct ctgcagtata
       61 agaggagggt tgggctggcg attcccaggg tagtgaacat gaatggtgtg gccagtattg
      121 gccgtcagct gccttagggt cctctgactg cgcccgatgc cctgggtgag acgagttgtt
      181 gaagagccac tgcccagtgt cagagaactg tggtctgcat ggcgcaccat cagtggatgg
      241 gtggtaggga tatttcctgg ggatgggggg atgtctgtag cactggagaa catgttgtca
      301 aactcaatga tgagatcatc ctcccggtca aagcgctcaa atcggaccaa gggagaagcg
      361 ttcatatcag gataatcctc atccaattcc atctcagagc catcgtcgtc atcgtcttca
      421 tctccctctt caccttcatc atcctcctga tcatcttcct catcttcctc ctcctcctcc
      481 tcttcatcct gactatcatc ctcatcctcg ttactgccac tgctgtcctc ttcctgagtg
      541 tgctcctcct catcctcagg gtccaggata ttcatggaat cttctcggtt ggcctgcaag
      601 gtggaagctt ggctgaggtt ggaaggagct tcatccatca gcacgtcctc ttgtgattca
      661 tcctctccac ttctgctcac tataattgta ctgttcccag atccgccatc cctctcaagc
      721 aactcatcta tcaggtcctc cagctcattc tcaacctgca tctcttgtga actgagcacc
      781 tcaggctgcc cagcaatcac cactgagtcg gtttcagcct ccccatccat gatatcccca
      841 tctgccacct ctgtctgagt gacatcatga tcctcctcct gcacttctgc ttccccaggc
      901 tcgcctgggt cctgctggtt gctactggaa tcttgagagg ctccttgggc atcctgctca
      961 gacttgttct tgctagaagc actcttgctg ccaaaaaggc tactgggctg gttcacaatc
     1021 cgggaaagtg tttccaaagg cttcagagca gcattgactg tgttggccat gttgggactg
     1081 gacaggtcta agctgtgagg tactctggcc aggtcattaa ccagtccctt cttcaggaaa
     1141 agccgaatga tgttgttcat gccattgtgc tgggtcttcg ctgtggcact gctgtagaag
     1201 ctggaggtgg aggggcagga ctccatgata gtactgatga tacacatcac tgcctgaagc
     1261 ctggcatgtt tctctgtact ctcagccata gccagtgccc gtccaagggc tgcttttact
     1321 tcattcacta gggccacctg ggcatctgtg ccactccctg cagcagccag gcttgcgagg
     1381 aacaggcggg ccaaggcagg ggtgtccttg tcttctgcat tctgggtatg tgggagcagg
     1441 tggtccagaa caaaagctag cacactgcag tcctctttga tcagttcaga ctggcccaca
     1501 gtgtagctgt agttggcaat cagggtagca ataccaacat aggacctcac caactctgcc
     1561 agaagacgaa ggatagtgga ggtaggcatt aaaggtttgc tgcccttgcc tttctgtttg
     1621 ccttcctcag aggcccggtc cccttcttta tctttcttcc catctcgaga ctcctctgga
     1681 gttgaagcct ctccttggga ggtcccagat gcggaagtct cagtggaggc accatctgca
     1741 gcaaagacct gactattaat ggaaaaacct gactgcgtat caaagtcact gctctgacgc
     1801 gtaagtgacc ggtactgctg gtatacatca tcacccatgt cttgcaggag ctggccaacc
     1861 tcttgggtca taaccccagg tttaggatca gatttatcgg cttcctctgg agcatggtat
     1921 gcagccagag cattcagcat atcatagatc acttccttga tagtatcagg gatgacaggc
     1981 agaggtgagg gcttcaaagg ggtggtcttc accagctgta cagcattagg gcctttaatt
     2041 ctaagattct caaattcatc atctgaagca gttcctgagc ctcgaggggc aggaagggcg
     2101 atgcggatac agcagttggc cacttctgtg aatatgtctg gattgcggca tgcggctggc
     2161 ccaaggacac gaaggatgta gttgatctcc cgagagccga ggctgccaga cacaacacca
     2221 gaggtagtgc taccagctcc acttgtagct gctgagcgaa caaccttttc catggtatga
     2281 cgaagggtac aggggtcctc aatgatgtgt cttaagagaa gggtgaccag gggagtaaac
     2341 ccattgaagc ctgagctctg ggtcaaattc aagatcatgc gggtactctt cagttctgca
     2401 aacatcatgg catatttgtg gtcccgggtg agcctcagac agagacgaag ggtggcatgc
     2461 aaagtatctg ggtccacagg gactcccagc atgctcacgc aggcccggat taaaacagtc
     2521 accatatctt ctgtcaagcc ctggatcagg atctccccga tttttgtttc ctccaggctt
     2581 gtctcctttt cagtgtttgt actctctagg gccaaagggg tatcattgcc tttattttct
     2641 ttacgtttga tatccatttc tttgctttct tccagcgtct tctctagttc ctgtccattg
     2701 ctgttttttg aatttttatt cagccgaggt accctgagga gagtcagcat cacagggcgt
     2761 cggttccctg tttcctcatt aacctgcacc attgtagtga attggaccgt gtatcttctt
     2821 cggcctgcag tgaatcgcac gcttgtctct ccagatttcc aggcagaatc aatagtgcta
     2881 ttgttgcttg cactgtaact acaccaacgc ccagagcgat catcaaacca gcgccagttg
     2941 ttgctgttgg attgcaggta cttagtcatc tgggctctcc tttttgagga gatggctgtc
     3001 ttttcataga aatcaatcag gagcaacact ggtgtgatcc actttggggt ctggacttcc
     3061 ttctgctcct tggctgcctg gaggcagggc tgaaccactt ccaagagttt gattaggaca
     3121 ttaaggatgc cacttgattc aaccacccaa gcacaaggta gcttcaactc ctcaaaaagt
     3181 agcgttaaaa gcaagattct agtagccaaa ttggaggcct ggggcagtgt ggccatctga
     3241 cttatccact ctgacacggt ttttgtgtca cttgttgtca ggggaagagc agctttgatc
     3301 aatacatcag cagcttccca cacctgattg actacttgct tcagaatcat gtcacgataa
     3361 tctgctccat tacgtttgat tgctgtcatg atcaggtcac acacacggta tactgtgtct
     3421 ggcagctcat caagaaggtg gaagcagcct ggcaacatag tatctgtgaa agtgtggagc
     3481 tcatcttgtt ccaacgggtc agcatcctgg aacttctcta gacatttagc ctcttcctcc
     3541 tcctgctttt cccgagcttt ccgttcctct tcctccttcc ggcaagcaac ttcctcaggt
     3601 gactctgccc tttgatccat tggaatatcc tgtcccagag acatagcaat tgctctcatc
     3661 atctggtcct cttcagacat gctgagatcc cgaacaactc ctcccatgat tggaggaggg
     3721 tgggttaaaa ggtactctgt ggcctgctcc atggtgctgg tgttcaacag tgcctccatt
     3781 gcatgttccc ttgtgaagcc catgtccatg agctgttgca gttgttgctg gttgacttga
     3841 ggttcccggc gggagccacc ttcctcttgc cctgtatcct cttctcctcg agacccctcc
     3901 ttctccttgc ttagtctctc tcgaatcaca ggttctcctc ggaggatgtg gcatagaatg
     3961 gccagcatcg attcagccat tcgtccacca tataccttca ggggtttccg gttccataag
     4021 tttttgatgc aagtaaaggc tgctttctga gttaccacaa ggaagcgcag tgcactgaac
     4081 tggggaaagt tctggacacc tccaggcaat ttggcaggca gcgaatgtgg agattcaagc
     4141 accgtggtgg gattcaccat cttctccacc agcataagcc aggcatctag gaattctcct
     4201 gtgccatcag gcaagtctga gtgttccaat ccctcagaaa caggaacttt acctcccatg
     4261 gacagagccc agttgaaagt ttcaaaaaga gcattgtggc ctccggagca gagaaatttt
     4321 tgcagcatga ggtggtaggg atacttcctc tcatcaaaca gcattgggga tgtgaaacca
     4381 actgaacaga tgaagaatgt cagcctgaat cggggagtag gtgtatatgg tgggggctgc
     4441 caagataacc ccttagtcaa gagcttagtg agagctgagg ctgttgatcg cgcggcaggt
     4501 gtcggtgctg tagtggtgct ggcagcatga tggctccttc tctggcggac aggagatccc
     4561 acacaaagtt taacaagaag tccaaatagc tcagcaagtg ctcggcctaa tctggaggaa
     4621 gctgataaca aaggcttgat ttgcttaatt ctggcagcca ttgctggtgt gattttagat
     4681 ttgcccttag agtctgaagc agtaggttca tctgtctcca tgggagccaa tgtgtcacca
     4741 tctagcccaa tgccttctaa taagccctgg gtagaagcat ccatacttcc agctgctcca
     4801 tcctgttccc catctgatct ttttccgccc tgggtcgtac ctgccttctc atcctttgga
     4861 accagtttct gcatatctgc ctggccaaat tcacacccag atggtaggct gtttggggta
     4921 cacagagaga ggaggacagt gctttcccac accagggaac agtataactg gctcagcttg
     4981 ctcaaaacac tcagacccaa ttgagagccc cactggttta cggagatgga acgaatttca
     5041 ctctgtccaa ctctgcaagt atgaacaaac atcatgatgt aggcatgggc agcagtgagt
     5101 gcatgcagca gaggtgtggc ctgggctgag agggtagcat cagcaacatt gcctgcgcaa
     5161 gccagttctc gcaacaacac tgagccccca ggggattcaa tggggcggtg taagggctcc
     5221 agggaggaga ggatggagtc caactgaagg agaccctctt gaaggacttt gggttcatgt
     5281 gacagtgtca atatggattt gcagacacct gcaacagcct gacaggcagc agatgtggga
     5341 aagtcaatgg gcagattggg aagacccaaa atggtaacca aaggcaacag tcctttctga
     5401 ttcacaaatt cctggcagtg gtcatctgtt gtattgttgc tcagaataga ttccacaaat
     5461 ttcatcacat taaggatgta atccatgagg ggaataggaa tacgttcctc tgtaccaaca
     5521 acctgctgat taggctcagt ttcattttgc tgggtagaat taaagctctg catggcctgt
     5581 acttcctctt cctcctcatc ctcactagag gcttcttctg cggcatgatt agaccttggg
     5641 ggaggagcag tggcagtgcc atctgccttc tggatcgatg gcttctgaca gatgtatttg
     5701 gggtcccttc caagattaca gatttcttca agtaacttga tgatggcagt cgttgcatct
     5761 gttttaaggg tgggctgatg tctcatgagc tcatcgacag cactccccag gttggatgca
     5821 gtatccccaa ggggatcaga acttctcctc ctccgcatgg ctgggaggta atctggagac
     5881 agaagaactt tgaagaggcg ttcaaaaggc tgacactgaa caaaagactg aagacctcgg
     5941 gcattcaaac agagtgcact gaatacattt gggagggagc caaggacttc acgggtagca
     6001 ggaacatctt tgataagcag tgcatgcagc atgacatctg tcaatccatt gtcctggagt
     6061 gaggagagca gtgatggttc ttgaaataca aacacagtca ccacttcagt agctaggagg
     6121 aagagtgatg ggccatagta ttctgcattg ctgatgatgt gtttcaggga ggtaggcaga
     6181 gaaccatcca tcacatgtcg tatgccatct gagaaagcag ggtcttggat ggccttcttg
     6241 aggaaattca acatggattt cagaagtgct gctcgttgtg gaatacactg gactccatcc
     6301 atatcagttt ccatttcctc tccttcttgt gtagtattgg gtctctggat ctttggcttg
     6361 atcacaaacg gacattcttt tcggcacaga tctacttcat gctcaagtct ataaatgaag
     6421 atagaaagtc cactatggga ttgaaaagct gccatatcca ggttggtgat aaggtcaacc
     6481 actctgacgg ctctggtgac aaatgttatc tggtcctgtt catcgccaag aaactttatg
     6541 accttcaata aggcttccat cattccacag gagaccaagg cttcaccacc agcatcgtag
     6601 ctggccagat ggtataaaaa agagaagaga gcagtggcaa actggtgagg gtatggatcc
     6661 atggaaggat caatcatggc ctggatacag ttccttacaa gcactggcaa aaatccatgg
     6721 taggaggcag ttccagtaca gtcaataata ctgctgagtt tgggagttct ctccaagtgg
     6781 acaattgatg ttaatgttcg taaagaagct gctttaatct ccataagctg cttatccgtt
     6841 atttgcagca cgtccactaa ctcctctatc aagccattat acaagatact gtttgctgat
     6901 tcctgcaagg cattggaata cactaatata gatattgcat gcagtctggc ctgaactgcc
     6961 tgcaatcgct tcctgtgatt agaaaagcca tgggccagtc gtatgtgtgt aaataacagc
     7021 atctgcttat ccttaggaat gctgtacatt ttggtaagag attccatgat ttcagaaggg
     7081 ctttctgaaa tcttgtcaag ttgctctatg tgaatataat gtagtgtgtt actagttgtc
     7141 cttttctcaa ttttgacctc ggccccagga tctgcataga attcaaagtg tagtgtagtt
     7201 gcactgggtg gatatttcat catatgcaag tctctgcaac attctgcaag tccaaagcca
     7261 ttctcctttc caccccagct ctctgccaaa tgttgtagcc gagttagcag cggggtcctc
     7321 ttgtcagatc ccagacgagt gatgtagttt gatcttttgc taaatacata taggagattg
     7381 aggactgcca gcaccacttg catatcagag gaagccaata aagttgtcaa atgctctatg
     7441 gaactgtaca gatgccggga aaagctgtac tcaatgagca aggctgtgaa gttcaacaca
     7501 gccaagagaa gcattttcag ttgctctctt tctggcctat cacatacgag catccatgac
     7561 atattctcca ctgtctgtcc agcatctgcc agtattccat cgaagcggtc caacaggtcc
     7621 acccagtgat ataactcgca ctttccaatg ttccatgttt tgatctgctg cagttccaag
     7681 agaagttgct catcattaca aactttgagt ttgtctatta aggctctgca gtctgcagga
     7741 gcctcagtag gtgtcttctt cagtttagtc ctgtctactt tcatggtgtc gactgaattg
     7801 gttcctttaa agcctgcttt tttgtacaaa gttggcatta taaaaaagca ttgcttatca
     7861 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg gggcccgagc
     7921 ttaagtaact aactaacagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg
     7981 gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg
     8041 ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg
     8101 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt
     8161 atttgatgcc tggcagttcc ctactctcgc ttagtagtta gacgtccccg agatccatgc
     8221 tagcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     8281 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     8341 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     8401 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     8461 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     8521 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     8581 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     8641 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     8701 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     8761 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     8821 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     8881 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     8941 cggggtctga cgctcagtgg aacggggccc aatctgaata atgttacaac caattaacca
     9001 attctgatta gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat
     9061 tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc
     9121 agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa
     9181 tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag
     9241 tgacgactga atccggtgag aatggcaaaa gtttatgcat ttctttccag acttgttcaa
     9301 caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc
     9361 gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag
     9421 gaatcgaatg caaccggcgc agggacactg ccagcgcatc aacaatattt tcacctgaat
     9481 caggatattc ttctaatacc tggaatgctg tttttccggg gatcgcagtg gtgagtaacc
     9541 atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca
     9601 gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt
     9661 tcagaaacaa ctctggcgca tcgggcttcc catacaagcg atagattgtc gcacctgatt
     9721 gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta
     9781 atcgcggcct cgacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta
     9841 tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc
     9901 agagattttg agacacgggc cagagctgca gctggatggc aaataatgat tttattttga
     9961 ctgatagtga cctgttcgtt gcaacaaatt gataagcaat gctttcttat aatgccaact
    10021 ttgtacaaga aagctgggtc gcggccgctt attaggccag cccaaagcct tcagagcact
    10081 cctggatagc caacagtagc atgtggcgga gcttctcaaa gctctcatag gcaggcagat
    10141 ccagctgatt aaaacatgtg tgagctgaag gcaggcgatc tgtggacctg tcatctcgat
    10201 ggatctgaaa cttctgaatg ccattcatgc cttcgagggc agcaaagcct tgcaggggta
    10261 ccttggaagt acccgtgaca aactggagga acttggcacg gtcagcttga tcgaaagaac
    10321 gcaatgctct ccagaaccac tggatctgaa tagagttgga ctggtacttg tggtattcag
    10381 tgttggattt cagatcatcg atgtcaatgg tgggcagtcc tgatataagc agctctaact
    10441 cctgctcagt gaagatggaa atgaggcgct ttggaatgat ctcatagaag ccttctaaga
    10501 aagccgccaa ctgcttgcgg atggctcctg tcattctcat ctggcatacc aggtgtacat
    10561 actccttctt attctcctct gttaccaaga tgttggcccc attgggtttg aggtcacgaa
    10621 cttcacaaac tccaaactct tggacctcag tgctgaaggt gaggtcatag cctagtgtgg
    10681 agacatcatt ttccagcaga taaaccagac cttggtagaa gtggtaatct tcactctcca
    10741 tatctgtata tctgactgac ttgcccaaga tgtgtttgta aaaggatcga gtaaagtagc
    10801 actccagaag acggttgtca tatacagctt tggccacaat gcgtccgaca aatttaaaat
    10861 aggacagatg gttggggttg cagtgggaag atggattgat ggtgtaggtg actcgatcac
    10921 caggtgaggt acggaacaag gcatacatag ggttaaacat ctctcgagag atgatcatat
    10981 accactcccg caggagcccg ccagcatcct gcccttcttc tccttcaaat actatataca
    11041 atcgattctt catttcttcg ggggatttgc gatgcagctc acgataggag tcttcaaaca
    11101 catggtcacg acggacatgc acagccatgt cttctttccg gagcccctca tctaaacgct
    11161 ccagctcttg gcggaaatat ttgcgcttga catcaaagtc gaggacacga atgtagtcta
    11221 ccaggacagc aaaaggccca tcagcaaggt gggtcgtgga ctgccgtagg atctggttta
    11281 acacagtgcg gtgagtctct gcaaagcgaa ggaacttctg tgtgtcaccg ctgccgctct
    11341 cccgctctgt ggcatggacc agaaagaagg cctcgacagc aggctgtagc actagcaccg
    11401 catgctggtc atgggattcc tctagttcct ttagacactc cccaagcatg tcccacagct
    11461 cgtccaaact cagctgctcg ctgagcaggg gtaactcagg tggtctttct tccttttcct
    11521 tctccccctg tggggttcca tcggaggcaa tggattgagt atcttgagca ctgggagatg
    11581 gctggtccac atccatgggt gattcctccc tccggacaga cgcctctgac tggctagact
    11641 ccgacgttgc tgcttgttgc tgtctccgcg ccctttgacc ctcacgtacc atttgtataa
    11701 tggcatcagc ctcagcctcc agctgccgaa cagctgcctg gatgctgcta gctgagccta
    11761 aaccggagga acctagcctg cctgtctgct tggctttctt gttagcccgg cgcgtgtcgt
    11821 cccggagctg gatgatgacc tgtagtaccc tcaagaagaa cttctgggta gatgtcttgg
    11881 atgtcaacat ggacatagaa ggcagctgga gctcccggcc acccaaaggt cgcttctgag
    11941 atgccacaat taccacattc tcagccatgt caaacctgct ctgcattttg cccttcagct
    12001 tggtggtctg tggctgctcc tcaggcaggc catcaggaga gagggtttca cattgggctc
    12061 gccgctgctg ctcgaggttg tattcccgca gctcggccag cagggtacct atttgtttac
    12121 aaagggtata acccagatgg cgggctccat tcagtagcag cttgagaaca gtgtcccggg
    12181 tcccagagtc cccccgggag agctgcagta gtacgttggc tgcatcctct aagccttcct
    12241 cagaacaaga gtgggatgtc aacacctcta cagagagctg tagctggttt tcagtgaggc
    12301 cagaggacac catcttaaag tctgtactgc tgctgccccc atcactcacc ttcgctggag
    12361 atttgctgcc cttagtagtg ggagaaattg aaacagtagt ggtagcagtc gtgggggtag
    12421 tcactgtggt cgaagcagct acgacaatgg tggaaatagc cgtggcagca accagggctg
    12481 gagcagaagt gacaggggtg ggtgcagtag ggggtgtggg cgtggtggag gcggcagtgg
    12541 tggtggtggt agatgtggtt gaggtggcag tggtggtgga ggaagcaccg ctgccagaat
    12601 tagcctgtgc ttctgacacc ttgttttctg ggagagcaat tgagatgaga gaaaggagtc
    12661 tgaggagttt ctcagttaag agagagctcc ggcggatgac tgggtgtgac aacatgttca
    12721 tgagctgccc cagtggagag gcctcgaggc tgtatggaga ggtttccccc tcaccgccag
    12781 cgctcactgg cactgacttc acggagttct tgcctttccg gctgacattc atgttgtcca
    12841 gttttaccaa taagtcccag aagtctgtgc aaatgccact gctggaggac tgtgaggagc
    12901 atgggctaca ggccttattg cccctttccc gatcactctc acagtttgtt tctttggtcc
    12961 gctgctgtgt gaagtggctg ggaaatacct tggccaattg aatgagtgta tccaaaacgt
    13021 gtctgcagac aacaggagca gcttggggat ggatgtggac ggtggagcca ccgcttgcat
    13081 gcttctcggt atgtttacgc ccccctgaac gctggatctg aaatatatta gtcctgcagc
    13141 ctagggctgc atccatggat actgagagcc aggaaagctg gttggagctc tttgactcca
    13201 tcttgtgtag caggtccagg ggacggttct catggctact tgacccacag ctcttgctcg
    13261 actttttgcc cttttcctca cttgtagtga gtttgggtgt ttcaatgcat agctcactct
    13321 cactgctgcg ctgcaagatg gagagcagac tgcggatgac ccagtggcgg gtctgggcat
    13381 ggtagcagag atttctcagt actcggtgta gacggctagt attgagcttt ggctcatcca
    13441 caaaaagtag gaccaagaga caagaaaggg cttcgtggtc cagaaggagc cgtcctcgga
    13501 ggcggaggag agtatctaca ttactgccag aaggcctgtt atggctgctg ctacccccca
    13561 tctggaaggt gccacctctc tgcacagcaa ggcgagtata ctggacccca cggttgccac
    13621 ttaagcgact ggtgaaagcc gggcttcgga gaatagcaga gagtgcggag gtgctactgt
    13681 gcccaaacag acgctcatgc atgagctgtc gctgccgggc ttcttgctct cgtctcaggg
    13741 cttgagcctc agctgcaatg tcaggtggca tcacagctaa cacactgtcc tccatatcct
    13801 ctaggacact acggcgcagg tctgagggca gagtctggat gaaggtcaca gggtccatag
    13861 gggtgtctga gctggcattc tgtgctagtt ctcgtcgctg ctgctcagct ctctgctgtg
    13921 ccagtacttc ctcttggatc gcgggtggca gtgccgcaag aaactcggga ctcacctcag
    13981 tcacaccagg attccccacc actgcaggcg ctgagctatt tgtggagggg gcagtccggg
    14041 ttggtggacg aatgcctagc tggttctgta gaacttcccg acggatgtca tcaggcaggg
    14101 cagccagaaa agaggggtcc acaccttcag ggagactgat acccgcaagg ggatcttctt
    14161 cttcactgct tgttgaaggc aacggctctt ctaggattcc tcgggaatcg gcgccagaaa
    14221 tggccacggc agaatctctg gtggaagagc tctcagagga tgcaggtggg gagctgtcct
    14281 caggtggctg ggcagattcc cctgacccat cactcctgcc ttgcacttcc gccacagctg
    14341 gtggggcatc cccaggagtg gagctgcctg ctctgggctg ctcagaactg ccagctgctg
    14401 aagtgtcacc cacagcctcc tctaaggtac aggaagccag gctgcttgta tccataggag
    14461 agccttctag ctgactgctg acagccgtca gtgcatcaga atcctcagct ctctctgggg
    14521 aaagtgaggt tatagtggga gctggggata actccatctg agttgtttct gcttccccac
    14581 ttggccttgt gggacccaat tcctctggct ctacaggcat caatagctgt gtagagctgc
    14641 ccccttctcc agctggagac tgcagctcct gaggaacctc tcccaaagct ggtggggttg
    14701 ggagtgttgg ttgctgttgt gaggattgca gagtgccaag ggtctccttg gactcagatg
    14761 tagctgcatc agttgaagat ggggttgttg ggtagctgtc aggcataggc gtcccatctg
    14821 agagattctg ttcagtggag tcatttgact tagatgcagt acactgtgca ctctgatccc
    14881 tgttctcctt atcttctttg cctttatcag ttatctttgt ttcttcctca gccagttgtt
    14941 tcctgcgctt ctctcgcctt tcttccagct cctcatccct caggaattcc aggtgattga
    15001 caatggacac tttaaccact gaaacacagt catgcatgct ctcagcatcg agaactttgc
    15061 attcttctgt ccagcgggtc agggctgtgg ggatgctgga cagggttcct gcttggctgg
    15121 tagctgtgct ctgatcatgg aaaaagtcat ccagcagctc atcatcagaa cgggcgatga
    15181 tgtggacgtc atcgttgcct accaggaggc gggcccgacc ccggctttgt aggggaagg
//
