LOCUS       sequence-404042-        9325 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9325)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0903
FEATURES             Location/Qualifiers
     source          1..9325
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2263..2851
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2316..2336)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2734..2745)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2923..3156
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3234..3355
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3271..3290)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3325..3344
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3395..3530
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3550..3569)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3551..3569)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3579..3596)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3579..3595)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3588..3610)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3737..4192
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3824..3843)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4034..4055
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4218..4322
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4323..4391,4392..5183)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4541..4560)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5354..5942
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5843..5862
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6096..6113
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6230..6251
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6266..6271,6272..6289,6290..6296)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6304..6320
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6309..6331
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6328..6344
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6328..6344
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6363..6383
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6365..6383
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6411..6637
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6638..6818
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6865..6990
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7483..7716
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7901..7945
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8176..8386
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8198..8394
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8245..8380
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8426..9028
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8426..8445)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8922..8942
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9071..9188
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggg
     1201 cagcgccagc ccgggtctga gcagcgtatc ccccagccac ctcctgctgc cccccgacac
     1261 ggtgtcgcgg acaggcttgg agaaggcggc agcgggggca gtgggtctcg agagacggga
     1321 ctggagtccc agtccacccg ccacgcccga gcagggcctg tccgccttct acctctccta
     1381 ctttgacatg ctgtaccctg aggacagcag ctgggcagcc aaggcccctg gggccagcag
     1441 tcgggaggag ccacctgagg agcctgagca gtgcccggtc attgacagcc aagccccagc
     1501 gggcagcctg gacttggtgc ccggcgggct gaccttggag gagcactcgc tggagcaggt
     1561 gcagtccatg gtggtgggcg aagtgctcaa ggacatcgag acggcctgca agctgctcaa
     1621 catcaccgca gatcccatgg actggagccc cagcaatgtg cagaagtggc tcctgtggac
     1681 agagcaccaa taccggctgc cccccatggg caaggccttc caggagctgg cgggcaagga
     1741 gctgtgcgcc atgtcggagg agcagttccg ccagcgctcg cccctgggtg gggatgtgct
     1801 gcacgcccac ctggacatct ggaagtcagc ctcgaccagt gaggagagct ggaccgacag
     1861 cgaggtggac tcatcatgct ccgggcagcc catccacctg tggcagttcc tcaaggagtt
     1921 gctactcaag ccccacagct atggccgctt cattaggtgg ctcaacaagg agaagggcat
     1981 cttcaaaatt gaggactcag cccaggtggc ccggctgtgg ggcatccgca agaaccgtcc
     2041 cgccatgaac tacgacaagc tgagccgctc catccgccag tattacaaga agggcatcat
     2101 ccggaagcca gacatctccc agcgcctcgt ctaccagttc gtgcacccca tctagtaatg
     2161 aactagtccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
     2221 cgaaggttta cgtcgcctgt ccaggcgacg cgttaagtcg acaatcaacc tctggattac
     2281 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     2341 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     2401 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     2461 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     2521 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     2581 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     2641 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     2701 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     2761 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     2821 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
     2881 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     2941 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     3001 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     3061 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     3121 tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc
     3181 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     3241 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     3301 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     3361 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     3421 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     3481 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     3541 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     3601 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     3661 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     3721 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     3781 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     3841 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     3901 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     3961 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     4021 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     4081 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     4141 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     4201 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     4261 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     4321 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     4381 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     4441 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     4501 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     4561 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     4621 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     4681 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     4741 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     4801 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     4861 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     4921 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     4981 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     5041 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     5101 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     5161 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     5221 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     5281 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     5341 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5401 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     5461 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     5521 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     5581 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     5641 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5701 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     5761 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     5821 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5881 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5941 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     6001 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     6061 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     6121 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     6181 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     6241 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     6301 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     6361 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
     6421 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
     6481 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
     6541 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
     6601 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
     6661 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     6721 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     6781 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     6841 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     6901 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     6961 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     7021 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     7081 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     7141 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     7201 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     7261 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     7321 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     7381 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     7441 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     7501 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     7561 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     7621 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     7681 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     7741 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     7801 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     7861 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     7921 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     7981 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     8041 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     8101 cccaaccccg aggggaccca tgcattgcat ctcaattagt cagcaaccag gtgtggaaag
     8161 tccccaggct ccccagcagg cagaagtatg caaagcatgc gtctcaatta gtcagcaacc
     8221 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     8281 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     8341 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt
     8401 tctagaggta ccaccggagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac
     8461 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     8521 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     8581 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     8641 cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     8701 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     8761 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     8821 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     8881 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     8941 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     9001 catgacccgc aagcccggtg ccagttaacg aattctagat cttgagacaa atggcagtat
     9061 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     9121 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     9181 aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg ctcgaggcct
     9241 gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt
     9301 ctaggtcttg aaaggagtgg gaatt
//
