LOCUS       sequence-404044-        6507 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Envelope protein for producing lentiviral and MuLV retroviral 
            particles. Use in conjunction with a packaging vector such as 
            pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Stewart SA, Dykxhoorn DM, Palliser D, Mizuno H, Yu EY, An DS, 
            Sabatini DM, Chen IS, Hahn WC, Sharp PA, Weinberg RA, Novina CD
  TITLE     Lentivirus-delivered stable gene silencing by RNAi in primary cells.
  JOURNAL   RNA 2003 Apr;9(4):493-501.
  PUBMED    12649500
REFERENCE   2  (bases 1 to 6507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA"; date:
            "2003-04"; volume: "9(4)"; pages: "493-501"
COMMENT     Sequence Label: pCMV-VSV-G
FEATURES             Location/Qualifiers
     source          1..6507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(340..356)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(340..356)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(353..375)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    364..380
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(388..394,395..412,413..418))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    433..454
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(571..588)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(742..1330)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(822..841)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1501..2292,2293..2361))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2124..2143
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2362..2466)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2492..2947)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2629..2650)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2841..2860
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3074..3096
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3088..3105
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3089..3105
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3115..3134
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3115..3133
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        3254..3633
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3634..3837
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3788..3808
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3834..3858
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          3949..4521
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             join(4607..4654,4655..6142)
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     primer_bind     complement(6369..6388)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6434..6489
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6488..6507)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc
       61 catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag
      121 ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta gatttttttt
      181 atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta
      241 ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta
      301 tggagatccc tcgacggatc ggccgcaatt cgtaatcatg tcatagctgt ttcctgtgtg
      361 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
      421 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
      481 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
      541 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
      601 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
      661 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
      721 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
      781 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
      841 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
      901 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
      961 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     1021 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     1081 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     1141 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     1201 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     1261 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     1321 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     1381 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     1441 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     1501 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     1561 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     1621 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     1681 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     1741 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     1801 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     1861 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     1921 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     1981 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     2041 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     2101 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     2161 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     2221 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     2281 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     2341 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     2401 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     2461 tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa
     2521 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     2581 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     2641 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     2701 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     2761 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     2821 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     2881 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     2941 ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     3001 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     3061 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac
     3121 gactcactat agggcgaatt ggagctccac cgcggtggcg gccgctctag agagcttggc
     3181 ccattgcata cgttgtatcc atatcataat atgtacattt atattggctc atgtccaaca
     3241 ttaccgccat gttgacattg attattgact agttattaat agtaatcaat tacggggtca
     3301 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     3361 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     3421 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     3481 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     3541 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     3601 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     3661 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     3721 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     3781 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
     3841 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
     3901 caccgggacc gatccagcct ccggtcgacc gatcctgaga acttcagggt gagtttgggg
     3961 acccttgatt gttctttctt tttcgctatt gtaaaattca tgttatatgg agggggcaaa
     4021 gttttcaggg tgttgtttag aatgggaaga tgtcccttgt atcaccatgg accctcatga
     4081 taattttgtt tctttcactt tctactctgt tgacaaccat tgtctcctct tattttcttt
     4141 tcattttctg taactttttc gttaaacttt agcttgcatt tgtaacgaat ttttaaattc
     4201 acttttgttt atttgtcaga ttgtaagtac tttctctaat cacttttttt tcaaggcaat
     4261 cagggtatat tatattgtac ttcagcacag ttttagagaa caattgttat aattaaatga
     4321 taaggtagaa tatttctgca tataaattct ggctggcgtg gaaatattct tattggtaga
     4381 aacaactaca ccctggtcat catcctgcct ttctctttat ggttacaatg atatacactg
     4441 tttgagatga ggataaaata ctctgagtcc aaaccgggcc cctctgctaa ccatgttcat
     4501 gccttcttct ctttcctaca gctcctgggc aacgtgctgg ttgttgtgct gtctcatcat
     4561 tttggcaaag aattcctcga cggatccctc gaggaattct gacactatga agtgcctttt
     4621 gtacttagcc tttttattca ttggggtgaa ttgcaagttc accatagttt ttccacacaa
     4681 ccaaaaagga aactggaaaa atgttccttc taattaccat tattgcccgt caagctcaga
     4741 tttaaattgg cataatgact taataggcac agccttacaa gtcaaaatgc ccaagagtca
     4801 caaggctatt caagcagacg gttggatgtg tcatgcttcc aaatgggtca ctacttgtga
     4861 tttccgctgg tatggaccga agtatataac acattccatc cgatccttca ctccatctgt
     4921 agaacaatgc aaggaaagca ttgaacaaac gaaacaagga acttggctga atccaggctt
     4981 ccctcctcaa agttgtggat atgcaactgt gacggatgcc gaagcagtga ttgtccaggt
     5041 gactcctcac catgtgctgg ttgatgaata cacaggagaa tgggttgatt cacagttcat
     5101 caacggaaaa tgcagcaatt acatatgccc cactgtccat aactctacaa cctggcattc
     5161 tgactataag gtcaaagggc tatgtgattc taacctcatt tccatggaca tcaccttctt
     5221 ctcagaggac ggagagctat catccctggg aaaggagggc acagggttca gaagtaacta
     5281 ctttgcttat gaaactggag gcaaggcctg caaaatgcaa tactgcaagc attggggagt
     5341 cagactccca tcaggtgtct ggttcgagat ggctgataag gatctctttg ctgcagccag
     5401 attccctgaa tgcccagaag ggtcaagtat ctctgctcca tctcagacct cagtggatgt
     5461 aagtctaatt caggacgttg agaggatctt ggattattcc ctctgccaag aaacctggag
     5521 caaaatcaga gcgggtcttc caatctctcc agtggatctc agctatcttg ctcctaaaaa
     5581 cccaggaacc ggtcctgctt tcaccataat caatggtacc ctaaaatact ttgagaccag
     5641 atacatcaga gtcgatattg ctgctccaat cctctcaaga atggtcggaa tgatcagtgg
     5701 aactaccaca gaaagggaac tgtgggatga ctgggcacca tatgaagacg tggaaattgg
     5761 acccaatgga gttctgagga ccagttcagg atataagttt cctttataca tgattggaca
     5821 tggtatgttg gactccgatc ttcatcttag ctcaaaggct caggtgttcg aacatcctca
     5881 cattcaagac gctgcttcgc aacttcctga tgatgagagt ttattttttg gtgatactgg
     5941 gctatccaaa aatccaatcg agcttgtaga aggttggttc agtagttgga aaagctctat
     6001 tgcctctttt ttctttatca tagggttaat cattggacta ttcttggttc tccgagttgg
     6061 tatccatctt tgcattaaat taaagcacac caagaaaaga cagatttata cagacataga
     6121 gatgaaccga cttggaaagt aactcaaatc ctgcacaaca gattcttcat gtttggacca
     6181 aatcaacttg tgataccatg ctcaaagagg cctcaattat atttgagttt ttaattttta
     6241 tgaaaaaaaa aaaaaaaaac ggaattcctc gagggatccg tcgaggaatt cactcctcag
     6301 gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac
     6361 cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat
     6421 ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg
     6481 tgtctctcac tcggaaggac atatggg
//
