LOCUS       sequence-404045-       10288 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10288)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2072
FEATURES             Location/Qualifiers
     source          1..10288
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3226..3814
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3279..3299)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3697..3708)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3886..4119
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4197..4318
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4234..4253)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4288..4307
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4358..4493
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4513..4532)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4514..4532)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4542..4559)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4542..4558)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4551..4573)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4700..5155
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4787..4806)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4997..5018
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5181..5285
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5286..5354,5355..6146)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5504..5523)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6317..6905
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6806..6825
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7059..7076
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7193..7214
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7229..7234,7235..7252,7253..7259)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7267..7283
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7272..7294
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7291..7307
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7291..7307
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7326..7346
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7328..7346
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7374..7600
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7601..7781
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7828..7953
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8446..8679
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8864..8908
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9139..9349
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9161..9357
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9208..9343
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9389..9991
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9389..9408)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9885..9905
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10034..10151
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggc
     1201 ccgaagaccc cggcacagca tatatagcag tgacgaggat gatgaggact ttgagatgtg
     1261 tgaccatgac tatgatgggc tgcttcccaa gtctggaaag cgtcacttgg ggaaaacaag
     1321 gtggacccgg gaagaggatg aaaaactgaa gaagctggtg gaacagaatg gaacagatga
     1381 ctggaaagtt attgccaatt atctcccgaa tcgaacagat gtgcagtgcc agcaccgatg
     1441 gcagaaagta ctaaaccctg agctcatcaa gggtccttgg accaaagaag aagatcagag
     1501 agtgatagag cttgtacaga aatacggtcc gaaacgttgg tctgttattg ccaagcactt
     1561 aaaggggaga attggaaaac aatgtaggga gaggtggcat aaccacttga atccagaagt
     1621 taagaaaacc tcctggacag aagaggaaga cagaattatt taccaggcac acaagagact
     1681 ggggaacaga tgggcagaaa tcgcaaagct actgcctgga cgaactgata atgctatcaa
     1741 gaaccactgg aattctacaa tgcgtcggaa ggtcgaacag gaaggttatc tgcaggagtc
     1801 ttcaaaagcc agccagccag cagtggccac aagcttccag aagaacagtc atttgatggg
     1861 ttttgctcag gctccgccta cagctcaact ccctgccact ggccagccca ctgttaacaa
     1921 cgactattcc tattaccaca tttctgaagc acaaaatgtc tccagtcatg ttccataccc
     1981 tgtagcgtta catgtaaata tagtcaatgt ccctcagcca gctgccgcag ccattcagag
     2041 acactataat gatgaagacc ctgagaagga aaagcgaata aaggaattag aattgctcct
     2101 aatgtcaacc gagaatgagc taaaaggaca gcaggtgcta ccaacacaga accacacatg
     2161 cagctacccc gggtggcaca gcaccaccat tgccgaccac accagacctc atggagacag
     2221 tgcacctgtt tcctgtttgg gagaacacca ctccactcca tctctgccag cggatcctgg
     2281 ctccctacct gaagaaagcg cctcgccagc aaggtgcatg atcgtccacc agggcaccat
     2341 tctggataat gttaagaacc tcttagaatt tgcagaaaca ctccaattta tagattcttt
     2401 cttaaacact tccagtaacc atgaaaactc agacttggaa atgccttctt taacttccac
     2461 ccccctcatt ggtcacaaat tgactgttac aacaccattt catagagacc agactgtgaa
     2521 aactcaaaag gaaaatactg tttttagaac cccagctatc aaaaggtcaa tcttagaaag
     2581 ctctccaaga actcctacac cattcaaaca tgcacttgca gctcaagaaa ttaaatacgg
     2641 tcccctgaag atgctacctc agacaccctc tcatctagta gaagatctgc aggatgtgat
     2701 caaacaggaa tctgatgaat ctggaattgt tgctgagttt caagaaaatg gaccaccctt
     2761 actgaagaaa atcaaacaag aggtggaatc tccaactgat aaatcaggaa acttcttctg
     2821 ctcacaccac tgggaagggg acagtctgaa tacccaactg ttcacgcaga cctcgcctgt
     2881 ggcagatgca ccgaatattc ttacaagctc cgttttaatg gcaccagcat cagaagatga
     2941 agacaatgtt ctcaaagcat ttacagtacc taaaaacagg tccctggcga gccccttgca
     3001 gccttgtagc agtacctggg aacctgcatc ctgtggaaag atggaggagc agatgacatc
     3061 ttccagtcaa gctcgtaaat acgtgaatgc attctcagcc cggacgctgg tcatgtagta
     3121 atgaactagt ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     3181 ggacgacata aactccctat tggcctctct acgcgttaag tcgacaatca acctctggat
     3241 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     3301 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     3361 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     3421 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     3481 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     3541 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     3601 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     3661 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     3721 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     3781 acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca
     3841 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     3901 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     3961 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     4021 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     4081 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc
     4141 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     4201 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     4261 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     4321 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     4381 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     4441 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     4501 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     4561 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     4621 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     4681 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     4741 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     4801 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     4861 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     4921 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     4981 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     5041 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     5101 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     5161 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     5221 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     5281 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     5341 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     5401 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     5461 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     5521 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     5581 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     5641 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     5701 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     5761 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     5821 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     5881 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     5941 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     6001 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     6061 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     6121 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     6181 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6241 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6301 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6361 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6421 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     6481 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6541 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6601 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6661 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6721 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6781 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6841 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6901 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6961 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     7021 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     7081 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     7141 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     7201 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     7261 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     7321 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
     7381 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
     7441 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
     7501 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
     7561 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
     7621 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     7681 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     7741 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     7801 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     7861 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     7921 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     7981 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     8041 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     8101 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     8161 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     8221 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     8281 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     8341 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     8401 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     8461 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     8521 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     8581 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     8641 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     8701 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     8761 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     8821 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     8881 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     8941 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     9001 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     9061 cctcccaacc ccgaggggac ccatgcattg catctcaatt agtcagcaac caggtgtgga
     9121 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcgtctcaa ttagtcagca
     9181 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     9241 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     9301 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     9361 ctttctagag gtaccaccgg agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
     9421 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     9481 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     9541 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     9601 cgccgtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     9661 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     9721 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     9781 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     9841 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     9901 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     9961 gtgcatgacc cgcaagcccg gtgccagtta acgaattcta gatcttgaga caaatggcag
    10021 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
    10081 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
    10141 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagg
    10201 cctgcaggtg caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac
    10261 cttctaggtc ttgaaaggag tgggaatt
//
