LOCUS       sequence-404048-        9376 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9376)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9376)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1029
FEATURES             Location/Qualifiers
     source          1..9376
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    2314..2902
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2367..2387)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2785..2796)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2974..3207
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3285..3406
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3322..3341)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3376..3395
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3446..3581
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3601..3620)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3602..3620)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3630..3647)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3630..3646)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3639..3661)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3788..4243
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3875..3894)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4085..4106
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4269..4373
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4374..4442,4443..5234)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4592..4611)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5405..5993
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5894..5913
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6147..6164
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6281..6302
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6317..6322,6323..6340,6341..6347)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6355..6371
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6360..6382
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6379..6395
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6379..6395
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6414..6434
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6416..6434
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6462..6688
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6689..6869
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6916..7041
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7534..7767
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7952..7996
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8227..8437
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8249..8445
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8296..8431
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8477..9079
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8477..8496)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8973..8993
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9122..9239
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgat
     1201 ttacacttgt caccgagata agaactgtgt tattaataaa gtcaccagga atcgatgcca
     1261 atactgtcga ctccagaagt gctttgaagt gggaatgtcc aaagaatctg tcaggaatga
     1321 caggaacaag aaaaagaagg agacttcgaa gcaagaatgc acagagagct atgaaatgac
     1381 agctgagttg gacgatctca cagagaagat ccgaaaagct caccaggaaa ctttcccttc
     1441 actctgccag ctgggtaaat acaccacgaa ttccagtgct gaccatcgag tccgactgga
     1501 cctgggcctc tgggacaaat tcagtgaact ggccaccaag tgcattatta agatcgtgga
     1561 gtttgctaaa cgtctgcctg gtttcactgg cttgaccatc gcagaccaaa ttaccctgct
     1621 gaaggccgcc tgcctggaca tcctgattct tagaatttgc accaggtata ccccagaaca
     1681 agacaccatg actttctcag acggccttac cctaaatcga actcagatgc acaatgctgg
     1741 atttggtcct ctgactgacc ttgtgttcac ctttgccaac cagctcctgc ctttggaaat
     1801 ggatgacaca gaaacaggcc ttctcagtgc catctgctta atctgtggag accgccagga
     1861 ccttgaggaa ccgacaaaag tagataagct acaagaacca ttgctggaag cactaaaaat
     1921 ttatatcaga aaaagacgac ccagcaagcc tcacatgttt ccaaagatct taatgaaaat
     1981 cacagatctc cgtagcatca gtgctaaagg tgcagagcgt gtaattacct tgaaaatgga
     2041 aattcctgga tcaatgccac ctctcattca agaaatgctg gagaattctg aaggacatga
     2101 acccttgacc ccaagttcaa gtgggaacac agcagagcac agtcctagca tctcacccag
     2161 ctcagtggaa aacagtgggg tcagtcagtc accactcgtg caatagtaat gaactagtcc
     2221 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgaatacat
     2281 accgatctgt tgtcccgaac gcgttaagtc gacaatcaac ctctggatta caaaatttgt
     2341 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     2401 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     2461 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     2521 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     2581 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     2641 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     2701 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     2761 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     2821 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     2881 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
     2941 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     3001 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     3061 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     3121 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     3181 tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc
     3241 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     3301 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     3361 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     3421 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     3481 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     3541 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     3601 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     3661 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     3721 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     3781 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     3841 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     3901 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     3961 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     4021 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     4081 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     4141 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     4201 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     4261 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     4321 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     4381 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     4441 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     4501 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     4561 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     4621 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     4681 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     4741 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     4801 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     4861 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     4921 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     4981 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     5041 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     5101 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     5161 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     5221 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     5281 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     5341 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5401 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     5461 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     5521 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     5581 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     5641 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     5701 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     5761 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     5821 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     5881 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     5941 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6001 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     6061 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     6121 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     6181 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     6241 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     6301 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     6361 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     6421 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact
     6481 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
     6541 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
     6601 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt
     6661 atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg
     6721 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     6781 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     6841 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     6901 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     6961 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     7021 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     7081 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     7141 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     7201 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     7261 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     7321 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     7381 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     7441 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     7501 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     7561 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     7621 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     7681 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     7741 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     7801 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     7861 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     7921 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     7981 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     8041 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     8101 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     8161 gaggggaccc atgcattgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     8221 tccccagcag gcagaagtat gcaaagcatg cgtctcaatt agtcagcaac catagtcccg
     8281 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     8341 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     8401 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ttctagaggt
     8461 accaccggag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     8521 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     8581 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     8641 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg ccgtggcggt
     8701 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     8761 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     8821 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     8881 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     8941 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     9001 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     9061 caagcccggt gccagttaac gaattctaga tcttgagaca aatggcagta ttcatccaca
     9121 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     9181 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     9241 gggtttatta cagggacagc agagatccac tttggcgccg gctcgaggcc tgcaggtgca
     9301 aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt
     9361 gaaaggagtg ggaatt
//
