LOCUS       sequence-404049-       12439 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12439)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1349
FEATURES             Location/Qualifiers
     source          1..12439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5377..5965
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5430..5450)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5848..5859)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6037..6270
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6348..6469
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6385..6404)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6439..6458
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6509..6644
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6664..6683)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6665..6683)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6693..6710)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6693..6709)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6702..6724)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6851..7306
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6938..6957)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7148..7169
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7332..7436
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7437..7505,7506..8297)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7655..7674)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8468..9056
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8957..8976
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9210..9227
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9344..9365
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9380..9385,9386..9403,9404..9410)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9418..9434
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9423..9445
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9442..9458
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9442..9458
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9477..9497
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9479..9497
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9525..9751
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9752..9932
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9979..10104
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10597..10830
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11015..11059
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        11290..11500
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        11312..11508
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      11359..11494
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             11540..12142
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(11540..11559)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     12036..12056
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    12185..12302
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggc
     1201 ggacaccgac ctgttcatgg aatgtgagga ggaggagttg gagccatggc agaaaatcag
     1261 tgatgtcatt gaggactctg tagttgaaga ttataattca gtggataaaa ctaccacagc
     1321 tggcaatcct ttggtccagc aaggtggaca gccactcatc ctgacccaga atccagcccc
     1381 aggtctgggc acaatggtta ctcaaccagt attgaggcct gttcaggtca tgcagaatgc
     1441 caatcatgtg actagttccc ctgtggcctc acaaccaata tttatcacta cgcagggatt
     1501 tcctgtaagg aatgtccggc ctgtacaaaa tgcaatgaat caggttggga ttgtgctgaa
     1561 cgtacagcaa ggccaaacgg ttagaccaat tacactagtt ccagccccag gtacccagtt
     1621 tgttaagccg acagttggag ttccacaagt gttctcccag atgacccctg tgaggccagg
     1681 ctccacaatg cctgtgaggc ccaccaccaa caccttcacc accgtcatcc cggccactct
     1741 taccattcga agcaccgtcc cacagtccca gtcccagcag accaagtcca ctcccagcac
     1801 ttctaccact cccactgcca cacagccaac ctcactgggg caactagctg ttcagtctcc
     1861 aggccagtca aaccagacca cgaatcccaa gctagctccc tccttcccct ctccacctgc
     1921 agtgagcatt gccagctttg tcactgtgaa gcgacctggt gttacaggcg aaaatagcaa
     1981 tgaagtggcc aaattggtga atacccttaa caccatccct tccctgggcc agagtcctgg
     2041 gccagtggtg gtgtccaaca acagctctgc tcatggctct caaagaacca gcggacctga
     2101 gtcttcaatg aaagtgacct cttccatccc agtatttgac ctccaggatg gtggacggaa
     2161 aatatgtcca cgatgtaatg ctcaatttcg tgttactgaa gctttgagag gtcacatgtg
     2221 ttactgttgc ccagaaatgg ttgaatacca gaagaaagga aagtccctgg attcagaacc
     2281 cagtgtccca tcagcagcaa agcccccatc ccctgagaaa acagctcctg ttgcttccac
     2341 accctcttct acacctattc ctgctctgtc accgcctacc aaagtaccag aaccaaatga
     2401 gaacgtgggc gatgccgtcc agaccaaact cattatgctt gtagatgact tctactatgg
     2461 acgggatggt ggcaaagtag cccagctcac aaatttccct aaggtcgcca catctttccg
     2521 atgcccacat tgtaccaaaa ggctaaaaaa caatattcga ttcatgaacc atatgaaaca
     2581 ccacgtagaa ctcgatcagc agaacggtga ggtagatggt cacactatct gccagcactg
     2641 ttaccgccag ttttccactc ccttccagct tcagtgccac ttggaaaatg ttcatagtcc
     2701 ctatgaatct actaccaagt gcaagatctg tgaatgggcg tttgaaagtg agccactatt
     2761 tctccagcat atgaaggata ctcataagcc tggagagatg ccttatgttt gccaggtgtg
     2821 tcaatatcgc tcctcactct actctgaggt agatgtccat tttcggatga tccatgagga
     2881 tacccggcat ctgctctgcc cttattgcct gaaggtcttc aaaaatggca atgcattcca
     2941 acagcattac atgaggcacc agaagagaaa tgtttatcac tgcaacaaat gccggctgca
     3001 gtttctcttt gccaaggaca aaattgaaca caagcttcaa caccataaaa ccttccgtaa
     3061 acccaagcag ctggagggct tgaaaccagg caccaaggtg acaatccggg cttcccgagg
     3121 gcagccacga actgttcctg tatcctctaa tgatacacct cccagcgcct tgcaggaggc
     3181 agcaccgctg acctcctcaa tggaccctct gcctgtcttc ctttatcccc ctgtccagcg
     3241 cagcatccag aagagagctg ttaggaaaat gagtgtcatg ggccggcaga catgcctgga
     3301 gtgcagcttc gagatcccag acttccctaa tcatttccct acttacgtac actgctctct
     3361 gtgtcgctat agcacctgct gttctcgagc ttatgccaac cacatgatca acaatcatgt
     3421 tccacggaag agccccaagt atttggcttt gtttaaaaat tctgtgagtg gaatcaagct
     3481 ggcctgcact tcatgtacct ttgttacctc tgtgggcgat gctatggcca agcatttggt
     3541 attcaacccc tctcacagat ccagcagcat cctgccacgg ggactcactt ggatagctca
     3601 ctcaaggcat ggccagactc gtgaccgagt gcatgaccgg aacgtgaaga atatgtaccc
     3661 tcctccttcc ttccccacta acaaagctgc cactgtgaaa tctgcggggg ccaccccagc
     3721 tgagcctgaa gagctactaa ctcccttagc cccagcactc ccatcaccag cctcaactgc
     3781 aaccccacca ccaaccccca ctcacccgca ggctttagcc cttccaccgc tggctacaga
     3841 gggagccgaa tgtctgaatg ttgatgatca ggatgaaggg agcccagtca cccaagaacc
     3901 tgagctagca tcaggtggtg gtggtagtgg tggagttggc aaaaaggagc agctgtctgt
     3961 gaagaagctt cgagtagtac tgtttgctct atgctgcaat acagaacagg cagctgaaca
     4021 cttccgaaat ccccagcgac gtattcgccg ttggcttcga cgtttccagg cctcccaggg
     4081 ggagaatcta gagggcaaat atctgagctt tgaggcagaa gagaaactgg ctgagtgggt
     4141 gctaacccag cgcgaacaac agctacctgt aaatgaggag accttgttcc agaaggccac
     4201 caaaatagga cgttctttgg aaggggggtt taagatctcc tatgagtggg ctgtgcgttt
     4261 catgctgcgg caccacctga ctccccatgc ccggcgagct gtggcccaca ccctacctaa
     4321 ggatgtagca gagaatgcag gactcttcat tgattttgta caacggcaga ttcacaacca
     4381 ggacttaccc ttgtctatga ttgtggctat tgatgagatc tctttgttcc tggatacaga
     4441 ggtgctgagc agtgatgatc gaaaggagaa tgccctgcag acagtgggca caggggaacc
     4501 ttggtgtgat gtagtcctag ccattctggc agatggcact gtccttccca ccctggtttt
     4561 ctacagaggg cagatggatc agcctgctaa catgccagac tccatattgc tagaggcaaa
     4621 ggagagtggc tacagtgatg acgagatcat ggagctgtgg tcaactcgag tgtggcagaa
     4681 gcacacagct tgccagcgca gcaaaggcat gcttgtgatg gactgtcatc gcactcactt
     4741 gtcagaagag gtactggcta tgcttagtgc ctctagcact ttgcctgcag tggtcccagc
     4801 aggctgtagc tccaaaattc agccattaga tgtatgcatc aaaagaactg tcaagaactt
     4861 cctgcataaa aaatggaagg aacaggctcg ggaaatggca gatactgcat gtgattctga
     4921 tgtcctgctt cagctggtgc ttgtctggct gggtgaagtg ctaggtgtca ttggggactg
     4981 tccagagcta gttcagcgct ccttcctggt ggctagtgtt ctgcctggcc ccgatggcaa
     5041 cattaactca cctacaagaa atgctgacat gcaggaggag ctaattgcct ccctagagga
     5101 gcaactgaag ctgagtgggg aacattctga gtcttccact ccacgaccca gatcatctcc
     5161 tgaagagaca attgagcctg aaagtcttca ccagctcttt gagggtgaaa gtgagaccga
     5221 gtctttctat ggctttgaag aagctgacct agatctgatg gagatttagt aatgaactag
     5281 tccgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgatta
     5341 gcgttgacat acgtattcgt aacgcgttaa gtcgacaatc aacctctgga ttacaaaatt
     5401 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     5461 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     5521 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     5581 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     5641 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     5701 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     5761 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     5821 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     5881 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     5941 atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg
     6001 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     6061 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     6121 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     6181 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     6241 ccttttagtc agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta
     6301 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     6361 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     6421 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     6481 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     6541 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     6601 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     6661 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     6721 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     6781 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     6841 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     6901 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     6961 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     7021 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     7081 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     7141 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     7201 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     7261 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     7321 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     7381 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     7441 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     7501 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     7561 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     7621 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     7681 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     7741 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     7801 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     7861 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     7921 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     7981 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     8041 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     8101 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     8161 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     8221 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     8281 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     8341 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     8401 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     8461 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     8521 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     8581 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     8641 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     8701 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     8761 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     8821 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     8881 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     8941 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     9001 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     9061 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     9121 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     9181 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     9241 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     9301 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     9361 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     9421 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     9481 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
     9541 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     9601 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     9661 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
     9721 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
     9781 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     9841 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     9901 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     9961 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
    10021 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
    10081 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
    10141 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
    10201 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
    10261 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
    10321 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
    10381 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
    10441 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
    10501 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
    10561 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
    10621 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
    10681 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
    10741 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
    10801 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
    10861 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
    10921 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
    10981 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
    11041 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
    11101 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
    11161 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
    11221 cccgagggga cccatgcatt gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
    11281 ggctccccag caggcagaag tatgcaaagc atgcgtctca attagtcagc aaccatagtc
    11341 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
    11401 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
    11461 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctttctaga
    11521 ggtaccaccg gagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
    11581 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
    11641 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
    11701 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgccgtggc
    11761 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
    11821 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
    11881 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
    11941 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
    12001 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
    12061 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
    12121 ccgcaagccc ggtgccagtt aacgaattct agatcttgag acaaatggca gtattcatcc
    12181 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
    12241 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
    12301 ttcgggttta ttacagggac agcagagatc cactttggcg ccggctcgag gcctgcaggt
    12361 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
    12421 cttgaaagga gtgggaatt
//
