LOCUS       sequence-404050-        9340 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9340)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0846
FEATURES             Location/Qualifiers
     source          1..9340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    2278..2866
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2331..2351)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2749..2760)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2938..3171
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3249..3370
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3286..3305)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3340..3359
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3410..3545
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3565..3584)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3566..3584)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3594..3611)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3594..3610)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3603..3625)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3752..4207
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3839..3858)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4049..4070
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4233..4337
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4338..4406,4407..5198)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4556..4575)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5369..5957
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5858..5877
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6111..6128
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6245..6266
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6281..6286,6287..6304,6305..6311)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6319..6335
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6324..6346
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6343..6359
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6343..6359
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6378..6398
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6380..6398
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6426..6652
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6653..6833
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6880..7005
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7498..7731
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7916..7960
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8191..8401
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8213..8409
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8260..8395
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8441..9043
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8441..8460)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8937..8957
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9086..9203
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgag
     1201 cagcgaggcc gagacccagc agccgcccgc cgcccccccc gccgcccccg ccctcagcgc
     1261 cgccgacacc aagcccggca ctacgggcag cggcgcaggg agcggtggcc cgggcggcct
     1321 cacatcggcg gcgcctgccg gcggggacaa gaaggtcatc gcaacgaagg ttttgggaac
     1381 agtaaaatgg ttcaatgtaa ggaacggata tggtttcatc aacaggaatg acaccaagga
     1441 agatgtattt gtacaccaga ctgccataaa gaagaataac cccaggaagt accttcgcag
     1501 tgtaggagat ggagagactg tggagtttga tgttgttgaa ggagaaaagg gtgcggaggc
     1561 agcaaatgtt acaggtcctg gtggtgttcc agttcaaggc agtaaatatg cagcagaccg
     1621 taaccattat agacgctatc cacgtcgtag gggtcctcca cgcaattacc agcaaaatta
     1681 ccagaatagt gagagtgggg aaaagaacga gggatcggag agtgctcccg aaggccaggc
     1741 ccaacaacgc cggccctacc gcaggcgaag gttcccacct tactacatgc ggagacccta
     1801 tgggcgtcga ccacagtatt ccaaccctcc tgtgcaggga gaagtgatgg agggtgctga
     1861 caaccagggt gcaggagaac aaggtagacc agtgaggcag aatatgtatc ggggatatag
     1921 accacgattc cgcaggggcc ctcctcgcca aagacagcct agagaggacg gcaatgaaga
     1981 agataaagaa aatcaaggag atgagaccca aggtcagcag ccacctcaac gtcggtaccg
     2041 ccgcaacttc aattaccgac gcagacgccc agaaaaccct aaaccacaag atggcaaaga
     2101 gacaaaagca gccgatccac cagctgagaa ttcgtccgct cccgaggctg agcagggcgg
     2161 ggctgagtag taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat
     2221 atcttgtgga aaggacgatt cttctgcagg agttcggtat tgacgcgtta agtcgacaat
     2281 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     2341 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     2401 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     2461 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     2521 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     2581 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     2641 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     2701 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     2761 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     2821 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac
     2881 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     2941 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     3001 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     3061 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     3121 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata
     3181 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     3241 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     3301 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     3361 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     3421 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     3481 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     3541 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     3601 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     3661 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     3721 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     3781 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     3841 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     3901 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     3961 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4021 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4081 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     4141 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     4201 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     4261 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     4321 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     4381 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     4441 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     4501 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     4561 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     4621 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     4681 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     4741 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     4801 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     4861 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     4921 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     4981 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     5041 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     5101 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     5161 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     5221 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     5281 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     5341 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     5401 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     5461 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     5521 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     5581 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     5641 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     5701 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     5761 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     5821 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     5881 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     5941 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     6001 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     6061 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     6121 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     6181 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     6241 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     6301 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     6361 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     6421 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
     6481 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
     6541 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
     6601 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
     6661 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     6721 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     6781 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     6841 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     6901 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     6961 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     7021 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     7081 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     7141 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     7201 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     7261 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     7321 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
     7381 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     7441 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     7501 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     7561 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     7621 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     7681 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     7741 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     7801 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     7861 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     7921 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     7981 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     8041 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     8101 gtttcagacc cacctcccaa ccccgagggg acccatgcat tgcatctcaa ttagtcagca
     8161 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcgtctc
     8221 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     8281 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     8341 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     8401 ttttgcaaaa agctttctag aggtaccacc ggagcttacc atgaccgagt acaagcccac
     8461 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     8521 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     8581 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     8641 ggacgacggc gccgccgtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     8701 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     8761 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     8821 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     8881 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     8941 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     9001 accgcgcacc tggtgcatga cccgcaagcc cggtgccagt taacgaattc tagatcttga
     9061 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     9121 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     9181 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc
     9241 gccggctcga ggcctgcagg tgcaaagatg gataaagttt taaacagaga ggaatctttg
     9301 cagctaatgg accttctagg tcttgaaagg agtgggaatt
//
