LOCUS       sequence-404053-       11272 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11272)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0722
FEATURES             Location/Qualifiers
     source          1..11272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4210..4798
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4263..4283)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4681..4692)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4870..5103
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5181..5302
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5218..5237)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5272..5291
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5342..5477
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5497..5516)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5498..5516)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5526..5543)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5526..5542)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5535..5557)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5684..6139
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5771..5790)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5981..6002
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6165..6269
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6270..6338,6339..7130)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6488..6507)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7301..7889
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7790..7809
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8043..8060
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8177..8198
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8213..8218,8219..8236,8237..8243)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8251..8267
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8256..8278
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8275..8291
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8275..8291
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8310..8330
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8312..8330
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8358..8584
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8585..8765
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8812..8937
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9430..9663
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9848..9892
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10123..10333
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        10145..10341
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10192..10327
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10373..10975
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10373..10392)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10869..10889
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    11018..11135
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggc
     1201 agaagatgat ccatatttgg gaaggcctga acaaatgttt catttggatc cttctttgac
     1261 tcatacaata tttaatccag aagtatttca accacagatg gcactgccaa cagatggccc
     1321 ataccttcaa atattagagc aacctaaaca gagaggattt cgtttccgtt atgtatgtga
     1381 aggcccatcc catggtggac tacctggtgc ctctagtgaa aagaacaaga agtcttaccc
     1441 tcaggtcaaa atctgcaact atgtgggacc agcaaaggtt attgttcagt tggtcacaaa
     1501 tggaaaaaat atccacctgc atgcccacag cctggtggga aaacactgtg aggatgggat
     1561 ctgcactgta actgctggac ccaaggacat ggtggtcggc ttcgcaaacc tgggtatact
     1621 tcatgtgaca aagaaaaaag tatttgaaac actggaagca cgaatgacag aggcgtgtat
     1681 aaggggctat aatcctggac tcttggtgca ccctgacctt gcctatttgc aagcagaagg
     1741 tggaggggac cggcagctgg gagatcggga aaaagagcta atccgccaag cagctctgca
     1801 gcagaccaag gagatggacc tcagcgtggt gcggctcatg tttacagctt ttcttccgga
     1861 tagcactggc agcttcacaa ggcgcctgga acccgtggta tcagacgcca tctatgacag
     1921 taaagccccc aatgcatcca acttgaaaat tgtaagaatg gacaggacag ctggatgtgt
     1981 gactggaggg gaggaaattt atcttctttg tgacaaagtt cagaaagatg acatccagat
     2041 tcgattttat gaagaggaag aaaatggtgg agtctgggaa ggatttggag atttttcccc
     2101 cacagatgtt catagacaat ttgccattgt cttcaaaact ccaaagtata aagatattaa
     2161 tattacaaaa ccagcctctg tgtttgtcca gcttcggagg aaatctgact tggaaactag
     2221 tgaaccaaaa cctttcctct actatcctga aatcaaagat aaagaagaag tgcagaggaa
     2281 acgtcagaag ctcatgccca atttttcgga tagtttcggc ggtggtagtg gtgctggagc
     2341 tggaggcgga ggcatgtttg gtagtggcgg tggaggaggg ggcactggaa gtacaggtcc
     2401 agggtatagc ttcccacact atggatttcc tacttatggt gggattactt tccatcctgg
     2461 aactactaaa tctaatgctg ggatgaagca tggaaccatg gacactgaat ctaaaaagga
     2521 ccctgaaggt tgtgacaaaa gtgatgacaa aaacactgta aacctctttg ggaaagttat
     2581 tgaaaccaca gagcaagatc aggagcccag cgaggccacc gttgggaatg gtgaggtcac
     2641 tctaacgtat gcaacaggaa caaaagaaga gagtgctgga gttcaggata acctctttct
     2701 agagaaggct atgcagcttg caaagaggca tgccaatgcc cttttcgact acgcggtgac
     2761 aggagacgtg aagatgctgc tggccgtcca gcgccatctc actgctgtgc aggatgagaa
     2821 tggggacagt gtcttacact tagcaatcat ccaccttcat tctcaacttg tgagggatct
     2881 actagaagtc acatctggtt tgatttctga tgacattatc aacatgagaa atgatctgta
     2941 ccagacgccc ttgcacttgg cagtgatcac taagcaggaa gatgtggtgg aggatttgct
     3001 gagggctggg gccgacctga gccttctgga ccgcttgggt aactctgttt tgcacctagc
     3061 tgccaaagaa ggacatgata aagttctcag tatcttactc aagcacaaaa aggcagcact
     3121 acttcttgac caccccaacg gggacggtct gaatgccatt catctagcca tgatgagcaa
     3181 tagcctgcca tgtttgctgc tgctggtggc cgctggggct gacgtcaatg ctcaggagca
     3241 gaagtccggg cgcacagcac tgcacctggc tgtggagcac gacaacatct cattggcagg
     3301 ctgcctgctc ctggagggtg atgcccatgt ggacagtact acctacgatg gaaccacacc
     3361 cctgcatata gcagctggga gagggtccac caggctggca gctcttctca aagcagcagg
     3421 agcagatccc ctggtggaga actttgagcc tctctatgac ctggatgact cttgggaaaa
     3481 tgcaggagag gatgaaggag ttgtgcctgg aaccacgcct ctagatatgg ccaccagctg
     3541 gcaggtattt gacatattaa atgggaaacc atatgagcca gagtttacat ctgatgattt
     3601 actagcacaa ggagacatga aacagctggc tgaagatgtg aagctgcagc tgtataagtt
     3661 actagaaatt cctgatccag acaaaaactg ggctactctg gcgcagaaat taggtctggg
     3721 gatacttaat aatgccttcc ggctgagtcc tgctccttcc aaaacactta tggacaacta
     3781 tgaggtctct gggggtacag tcagagagct ggtggaggcc ctgagacaaa tgggctacac
     3841 cgaagcaatt gaagtgatcc aggcagcctc cagcccagtg aagaccacct ctcaggccca
     3901 ctcgctgcct ctctcgcctg cctccacaag gcagcaaata gacgagctcc gagacagtga
     3961 cagtgtctgc gacagcggcg tggagacatc cttccgcaaa ctcagcttta ccgagtctct
     4021 gaccagtggt gcctcactgc taactctcaa caaaatgccc catgattatg ggcaggaagg
     4081 acctctagaa ggcaaaattt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc
     4141 ttggctttat atatcttgtg gaaaggacga gtgcgctttg cttcaccgga acgtacgcgt
     4201 taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     4261 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     4321 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     4381 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     4441 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     4501 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     4561 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     4621 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     4681 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     4741 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt
     4801 cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     4861 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     4921 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     4981 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     5041 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     5101 gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     5161 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     5221 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     5281 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     5341 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     5401 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     5461 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     5521 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     5581 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     5641 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     5701 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     5761 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     5821 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     5881 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     5941 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     6001 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     6061 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     6121 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     6181 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     6241 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     6301 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     6361 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     6421 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     6481 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     6541 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     6601 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     6661 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     6721 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     6781 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     6841 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6901 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6961 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     7021 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     7081 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     7141 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     7201 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     7261 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     7321 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     7381 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     7441 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     7501 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     7561 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     7621 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     7681 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7741 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7801 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7861 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7921 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     7981 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     8041 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     8101 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     8161 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     8221 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     8281 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     8341 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
     8401 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     8461 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
     8521 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
     8581 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     8641 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     8701 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     8761 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     8821 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     8881 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     8941 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     9001 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     9061 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     9121 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     9181 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     9241 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
     9301 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     9361 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     9421 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     9481 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     9541 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     9601 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     9661 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     9721 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     9781 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     9841 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     9901 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     9961 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
    10021 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccatgc attgcatctc
    10081 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
    10141 agcatgcgtc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
    10201 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
    10261 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
    10321 ggaggcctag gcttttgcaa aaagctttct agaggtacca ccggagctta ccatgaccga
    10381 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
    10441 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
    10501 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
    10561 ggtgtgggtc gcggacgacg gcgccgccgt ggcggtctgg accacgccgg agagcgtcga
    10621 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
    10681 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
    10741 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
    10801 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
    10861 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
    10921 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcca gttaacgaat
    10981 tctagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
    11041 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
    11101 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
    11161 atccactttg gcgccggctc gaggcctgca ggtgcaaaga tggataaagt tttaaacaga
    11221 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa tt
//
