LOCUS       sequence-404078-       16294 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16294)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 16294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1889
FEATURES             Location/Qualifiers
     source          1..16294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      10350..10359
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    12151..12739
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(12204..12224)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12622..12633)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             12811..13044
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    13122..13243
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13159..13178)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13213..13232
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      13283..13418
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(13438..13457)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(13439..13457)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(13467..13484)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13467..13483)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13476..13498)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      13625..14080
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13712..13731)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13922..13943
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        14106..14210
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(14211..14279,14280..15071)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(14429..14448)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      15242..15830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15731..15750
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15984..16001
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    16118..16139
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(16154..16159,16160..16177,16178..16184)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16192..16208
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     16197..16219
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     16216..16232
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     16216..16232
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     16251..16271
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        16253..16271
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgtct ggcggcgccg cagagaagca
     4141 gagcagcact cccggttccc tgttcctctc gccgccggct cctgccccca agaatggctc
     4201 cagctccgat tcctccgtgg gggagaaact gggagccgcg gccgccgacg ctgtgaccgg
     4261 caggaccgag gagtacaggc gccgccgcca cactatggac aaggacagcc gtggggcggc
     4321 cgcgaccact accaccactg agcaccgctt cttccgccgg agcgtcatct gtgactccaa
     4381 tgccactgca ctggagcttc ccggccttcc tctttccctg ccccagccca gcatccccgc
     4441 ggctgtcccg cagagtgctc caccggagcc ccaccgggaa gagaccgtga ccgccaccgc
     4501 cacttcccag gtagcccagc agcctccagc cgctgccgcc cctggggaac aggccgtcgc
     4561 gggccctgcc ccctcgactg tccccagcag taccagcaaa gaccgcccag tgtcccagcc
     4621 tagccttgtg gggagcaaag aggagccgcc gccggcgaga agtggcagcg gcggcggcag
     4681 cgccaaggag ccacaggagg aacggagcca gcagcaggat gatatcgaag agctggagac
     4741 caaggccgtg ggaatgtcta acgatggccg ctttctcaag tttgacatcg aaatcggcag
     4801 aggctccttt aagacggtct acaaaggtct ggacactgaa accaccgtgg aagtcgcctg
     4861 gtgtgaactg caggatcgaa aattaacaaa gtctgagagg cagagattta aagaagaagc
     4921 tgaaatgtta aaaggtcttc agcatcccaa tattgttaga ttttatgatt cctgggaatc
     4981 cacagtaaaa ggaaagaagt gcattgtttt ggtgactgaa cttatgacgt ctggaacact
     5041 taaaacgtat ctgaaaaggt ttaaagtgat gaagatcaaa gttctaagaa gctggtgccg
     5101 tcagatcctt aaaggtcttc agtttcttca tactcgaact ccacctatca ttcaccgcga
     5161 tcttaaatgt gacaacatct ttatcaccgg ccctactggc tcagtcaaga ttggagacct
     5221 cggtctggca accctgaagc gggcttcttt tgccaagagt gtgataggta ccccagagtt
     5281 catggcccct gagatgtatg aggagaaata tgatgaatcc gttgacgttt atgcttttgg
     5341 gatgtgcatg cttgagatgg ctacatctga atatccttac tcggagtgcc aaaatgctgc
     5401 acagatctac cgtcgcgtga ccagtggggt gaagccagcc agttttgaca aagtagcaat
     5461 tcctgaagtg aaggaaatta ttgaaggatg catacgacaa aacaaagatg aaagatattc
     5521 catcaaagac cttttgaacc atgccttctt ccaagaggaa acaggagtac gggtagaatt
     5581 agcagaagaa gatgatggag aaaaaatagc cataaaatta tggctacgta ttgaagatat
     5641 taagaaatta aagggaaaat acaaagataa tgaagctatt gagttttctt ttgatttaga
     5701 gagagatgtc ccagaagatg ttgcacaaga aatggtagag tctgggtatg tctgtgaagg
     5761 tgatcacaag accatggcta aagctatcaa agacagagta tcattaatta agaggaaacg
     5821 agagcagcgg cagttggtac gggaggagca agaaaaaaaa aagcaggaag agagcagtct
     5881 caaacagcag gtagaacaat ccagtgcttc ccagacagga atcaagcagc tcccttctgc
     5941 tagcaccggc atacctactg cttctaccac ttcagcttca gtttctacac aagtagaacc
     6001 tgaagaacct gaggcagatc aacatcaaca actacagtac cagcaaccca gtatatctgt
     6061 gttatctgat gggacggttg acagtggtca gggatcctct gtcttcacag aatctcgagt
     6121 gagcagccaa cagacagttt catatggttc ccaacatgaa caggcacatt ctacaggcac
     6181 agtcccaggg catatacctt ctactgtcca agcacagtct cagccccatg gggtatatcc
     6241 accctcaagt gtgcctcagt ccatggcgca tccgtgtggg gggaccccaa catacccaga
     6301 atcacagata tttttcccaa ctattcatga acgtccagtt tctttttcac cacctcccac
     6361 ctgcccaccg aaagtagcca tttcccagcg gcgtaagagc acctccttcc tggaagccca
     6421 aactcaccac ttccaacccc tgctgaggac tgttggccaa agtcttcttc cacctggtgg
     6481 cagcccaact aactggacac cagaggccgt agttatgttg ggtactacag ccagtagagt
     6541 aactggagag tcatgtgaga tacaggtcca tcctatgttt gaaccatctc aagtttacag
     6601 tgactataga cctggactag tacttccaga agaagctcac tattttattc ctcaggaagc
     6661 agtgtatgta gctggggtac attaccaggc ccgggtggca gaacagtatg agggcattcc
     6721 atacaactca tcagtactgt caagtcctat gaaacagata cctgaacaga agccagtaca
     6781 agggggccct acttcaagtt ctgtctttga atttccatct ggacaggctt tcctggtagg
     6841 acaccttcag aatctaagat tagattctgg attgggtccg ggatctcccc tctctagtat
     6901 ttctgcacct atcagtacag atgctacacg tttgaaattt caccctgtct ttgttcctca
     6961 ttctgcgcct gctgtgttaa ctcataacaa tgagagcaga agcaactgtg tatttgaatt
     7021 tcatgttcac acaccaagct cctcttcagg agaaggaggt ggaattttac ctcagcgtgt
     7081 ttaccgaaat cggcaggttg cagtggactt gaatcaagaa gaactgcctc ctcaatcagt
     7141 tggattacat ggctacttgc agcctgtgac tgaagaaaag cataattacc atgccccaga
     7201 attgaccgtt tctgtggtag agcctatcgg acagaactgg ccaataggaa gcccagaata
     7261 ttccagtgat tcctcacaaa tcacttcttc agaccccagt gattttcagt cacctccccc
     7321 tacaggggga gcagctgcac cttttggctc tgacgtctca atgcccttta tccatctgcc
     7381 tcagacagtg ttacaagaat ccccactttt cttctgtttc ccccaaggaa ccacatctca
     7441 gcaggtctta actgcctcat tttcttcagg aggatctgca cttcatccac aggcacaggg
     7501 gcagagccag ggtcagccat cctcaagtag cttaacaggg gtttcatctt cccaacccat
     7561 acaacatcct cagcagcagc agggaataca gcagacagcc cctcctcaac agacagtgca
     7621 gtattcactt tcacagacat caacctccag tgaggccact actgcacagc cagtgagtca
     7681 gcctcaagct ccacaagtct tgcctcaagt atcagctgga aaacagggct tcccacctcg
     7741 actgccacca cagtacccag gagattcaaa tattgctccc tcttccaacg tggcttctgt
     7801 ttgcatccat tctacagtcc tatcccctcc catgccgaca gaagtactgg ctacacctgg
     7861 gtactttccc acagtggtgc agccttatgt ggaatcaaat cttttagttc ctatgggtgg
     7921 tgtaggagga caggttcaag tgtcccagcc aggagggagt ttagcacaag cccccactac
     7981 atcctcccag caagcagttt tggagagtac tcagggagtc tctcaggttg ctcctgcaga
     8041 gccagttgca gtagcacaga cccaagctac ccagccgacc actttggctt cctctgtaga
     8101 cagtgcacat tcagatgttg cttcaggtat gagtgatggc aatgagaacg tcccatcttc
     8161 cagtggaagg catgaaggaa gaactacaaa acggcattac cgaaaatctg taaggagtcg
     8221 ctctcgacat gaaaaaactt cacgcccaaa attaagaatt ttgaatgttt caaataaagg
     8281 agaccgagta gtagaatgtc aattagagac tcataatagg aaaatggtta cattcaaatt
     8341 tgacctagat ggtgacaacc ccgaggagat agcaacaatt atggtgaaca atgactttat
     8401 tctagcaata gagagagagt cgtttgtgga tcaagtgcga gaaattattg aaaaagctga
     8461 tgaaatgctc agtgaggatg tcagtgtgga accagagggt gatcagggat tggagagtct
     8521 acaaggaaag gatgactatg gcttttcagg ttctcagaaa ttggaaggag agttcaaaca
     8581 accaattcct gcgtcttcca tgccacagca aataggcatt cctaccagtt ctttaactca
     8641 agttgttcat tctgcgggaa ggcggtttat agtgagtcct gtgccagaaa gccgattacg
     8701 agaatcaaaa gttttcccca gtgaaataac agatacagtt gctgcctcta cagctcagag
     8761 ccctggaatg aacttgtctc actctgcatc atcccttagt ctacaacagg ccttttctga
     8821 acttagacgt gcccaaatga cagaaggacc caacacagca cctccaaact ttagtcatac
     8881 aggaccaaca tttccagtag tacctccttt cttaagtagc attgctggag tcccaaccac
     8941 agcagcagcc acagcaccag tccctgcaac aagcagccct cctaatgaca tttccacatc
     9001 agtaattcag tctgaggtta cagtgcccac tgaagagggg attgctggag ttgccaccag
     9061 cacaggtgtg gtaacttcag gtggtctccc cataccacct gtgtctgaat caccagtact
     9121 ttccagcgta gtttcaagta tcacaatacc tgcagttgtc tcaatatcta ctacatcccc
     9181 gtcacttcaa gtccccacat ccacatctga gatcgttgtt tctagtacag cactgtatcc
     9241 ttcagtaaca gtttcagcaa cttcagcctc tgcagggggc agtactgcta ccccaggtcc
     9301 taagcctcca gctgtagtat ctcagcaggc agcaggcagc actactgtgg gagccacatt
     9361 aacatcagtt tctaccacca cttcattccc aagcacagct tcacagctgt gcattcagct
     9421 tagcagcagt acttctactc ctactttagc tgaaaccgtg gtagttagcg cacactcact
     9481 agataagaca tctcatagca gtacaactgg attggctttc tccctctctg caccatcttc
     9541 ctcttcctct cctggagcag gagtgtctag ttatatttct cagcctggtg ggctgcatcc
     9601 tttggtcatt ccatcagtga tagcttctac tcctattctt ccccaagcag caggacctac
     9661 ttctacacct ttattacccc aagtacctag tatcccaccc ttggtacagc ctgttgccaa
     9721 tgtgcctgct gtacagcaga cactaattca tagtcagcct caaccagctt tgcttcccaa
     9781 ccagccccat actcattgtc ctgaagtaga ttctgataca caacccaaag ctcctggaat
     9841 tgatgacata aagactctag aagaaaagct gcggtctctg ttcagtgaac acagctcatc
     9901 tggagctcag catgcctctg tctcactgga gacctcacta gtcatagaga gcactgtcac
     9961 accaggcatc ccaactactg ctgttgcacc aagcaaactc ctgacttcta ccacaagtac
    10021 ttgcttacca ccaaccaatt taccactagg aacagttgct ttgccagtta caccagtggt
    10081 cacacctggg caagtttcta ccccagtcag cactactaca tcaggagtga aacctggaac
    10141 tgctccctcc aagccacctc taactaaggc tccggtgctg ccagtgggta ctgaacttcc
    10201 agcaggtact ctacccagcg agcagctgcc accttttcca ggaccttctc taacccagtc
    10261 ccagcaacct ctagaggatc ttgatgctca attgagaaga acacttagtc cagagatgat
    10321 cacagtgact tctgcggttg gtcctgtgtc catggcggct ccaacagcaa tcacagaagc
    10381 aggaacacag cctcagaagg gtgtttctca agtcaaagaa ggccctgtcc tagcaactag
    10441 ttcaggagct ggtgttttta agatgggacg atttcaggtt tctgttgcag cagacggtgc
    10501 ccagaaagag ggtaaaaata agtcagaaga tgcaaagtct gttcattttg aatccagcac
    10561 ctcagagtcc tcagtgctat caagtagtag tccagagagt accttggtga aaccagagcc
    10621 gaatggcata accatccctg gtatctcttc agatgtgcca gagagtgccc acaaaactac
    10681 tgcctcagag gcaaagtcag acactgggca gcctaccaag gttggacgtt ttcaggtgac
    10741 aactacagca aacaaagtgg gtcgtttctc tgtatcaaaa actgaggaca agatcactga
    10801 cacaaagaaa gaaggaccag tggcatctcc tccttttatg gatttggaac aagctgttct
    10861 tcctgctgtg ataccaaaga aagagaagcc tgaactgtca gagccttcac atctaaatgg
    10921 gccgtcttct gacccggagg ccgctttttt aagtagggat gtggatgatg gttccggtag
    10981 tccacactcg ccccatcagc tgagctcaaa gagccttcct agccagaatc taagtcaaag
    11041 ccttagtaat tcatttaact cctcttacat gagtagcgac aatgagtcag atatcgaaga
    11101 tgaagactta aagttagagc tgcgacgact acgagataaa catctcaaag agattcagga
    11161 cctgcagagt cgccagaagc atgaaattga atctttgtat accaaactgg gcaaggtgcc
    11221 ccctgctgtt attattcccc cagctgctcc cctttcaggg agaagacgac gacccactaa
    11281 aagcaaaggc agcaaatcta gtcgaagcag ttccttgggg aataaaagcc cccagctttc
    11341 aggtaacctg tctggtcaga gtgcagcttc agtcttgcac ccccagcaga ccctccaccc
    11401 tcctggcaac atcccagagt ccgggcagaa tcagctgtta cagcccctta agccatctcc
    11461 ctccagtgac aacctctatt cagccttcac cagtgatggt gccatttcag taccaagcct
    11521 ttctgctcca ggtcaaggaa ccagcagcac aaacactgtt ggggcaacag tgaacagcca
    11581 agccgcccaa gctcagcctc ctgccatgac gtccagcagg aagggcacat tcacagatga
    11641 cttgcacaag ttggtagaca attgggcccg agatgccatg aatctctcag gcaggagagg
    11701 aagcaaaggg cacatgaatt acgagggccc tggaatggca aggaagttct ctgcacctgg
    11761 gcaactgtgc atctccatga cctcgaacct gggtggctct gcccccatct ctgcagcatc
    11821 agctacctct ctaggtcact tcaccaagtc tatgtgcccc ccacagcagt atggctttcc
    11881 agctacccca tttggcgctc aatggagtgg gacgggtggc ccagcaccac agccacttgg
    11941 ccagttccaa cctgtgggaa ctgcctcctt gcagaatttc aacatcagca atttgcagaa
    12001 atccatcagc aaccccccag gctccaacct gcggaccact tagtaatgaa ctagtccgta
    12061 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg accgacggtg
    12121 cgacgtcttt cgttgacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
    12181 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
    12241 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
    12301 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
    12361 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
    12421 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
    12481 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
    12541 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
    12601 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
    12661 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
    12721 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
    12781 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
    12841 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
    12901 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
    12961 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
    13021 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
    13081 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
    13141 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    13201 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
    13261 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
    13321 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
    13381 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
    13441 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
    13501 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
    13561 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
    13621 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
    13681 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
    13741 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
    13801 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
    13861 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
    13921 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
    13981 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
    14041 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
    14101 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
    14161 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
    14221 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
    14281 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
    14341 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
    14401 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
    14461 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
    14521 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
    14581 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
    14641 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
    14701 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
    14761 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
    14821 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
    14881 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
    14941 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
    15001 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
    15061 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
    15121 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
    15181 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
    15241 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
    15301 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
    15361 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
    15421 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
    15481 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
    15541 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
    15601 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
    15661 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    15721 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    15781 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    15841 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
    15901 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
    15961 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
    16021 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
    16081 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
    16141 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
    16201 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
    16261 ctcactaaag ggaacaaaag ctggagctgc aagc
//
