LOCUS       sequence-404080-       10558 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10558)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1878
FEATURES             Location/Qualifiers
     source          1..10558
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6415..7003
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6468..6488)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6886..6897)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7075..7308
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7386..7507
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7423..7442)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7477..7496
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7547..7682
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7702..7721)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7703..7721)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7731..7748)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7731..7747)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7740..7762)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7889..8344
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7976..7995)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8186..8207
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8370..8474
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8475..8543,8544..9335)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8693..8712)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9506..10094
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9995..10014
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10248..10265
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10382..10403
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10418..10423,10424..10441,10442..10448)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10456..10472
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10461..10483
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10480..10496
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10480..10496
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10515..10535
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10517..10535
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggcg tcgggccgtg gagcttcttc
     4141 tcgctggttc tttactcggg aacagctgga gaacacgccg agccgccgct gcggagtgga
     4201 ggcggataaa gagctctcgt gccgccagca ggcggccaac ctcatccagg agatgggaca
     4261 gcgtctcaat gtctctcagc ttacaataaa cactgcgatt gtttatatgc acaggtttta
     4321 tatgcaccat tctttcacca aattcaacaa aaatataata tcgtctactg cattattttt
     4381 ggctgcaaaa gtggaagaac aggctcgaaa acttgaacat gttatcaaag tagcacatgc
     4441 ttgtcttcat cctctagagc cactgctgga tactaaatgt gatgcttacc ttcaacagac
     4501 tcaagaactg gttatacttg aaaccataat gctacaaact ctaggttttg agatcaccat
     4561 tgaacaccca cacacagatg tggtgaaatg tacccagtta gtaagagcaa gcaaggattt
     4621 ggcacagaca tcctatttca tggctaccaa cagtctgcat cttacaacct tctgtcttca
     4681 gtacaaacca acagtgatag catgtgtatg cattcatttg gcttgcaaat ggtccaattg
     4741 ggagatccct gtatcaactg atggaaagca ttggtgggaa tatgtggatc ctacagttac
     4801 tctagaatta ttagatgagc taacacatga gtttctacaa atattggaga aaacgcctaa
     4861 taggttgaag aagattcgaa actggagggc taatcaggca gctaggaaac caaaagtaga
     4921 tggacaggta tcagagacac cacttcttgg ttcatctttg gtccagaatt ccattttagt
     4981 agatagtgtc actggtgtgc ctacaaaccc aagttttcag aaaccatcta catcagcatt
     5041 ccctgcgcca gtacctctaa attcaggaaa tatttctgtt caagacagcc atacatctga
     5101 taatttgtca atgctagcaa caggaatgcc aagtacttca tacggtttat catcacacca
     5161 ggaatggcct caacatcaag actcagcaag gacagaacag ctatattcac agaaacagga
     5221 gacatctttg tctggtagcc agtacaacat caacttccag cagggacctt ctatatcact
     5281 gcattcagga ttacatcaca gacctgacaa aatttcagat cattcttctg ttaagcaaga
     5341 atatactcat aaagcaggga gcagtaaaca ccatgggcca atttccacta ctccaggaat
     5401 aattcctcag aaaatgtctt tagataaata tagagaaaag cgtaaactag aaactcttga
     5461 tctcgatgta agggatcatt atatagctgc ccaggtagaa cagcagcaca aacaagggca
     5521 gtcacaggca gccagcagca gttctgttac ttctcccatt aaaatgaaaa tacctatcgc
     5581 aaatactgaa aaatacatgg cagacaaaaa ggaaaagagt gggtcactga aattacggat
     5641 tccaatacca cccactgata aaagcgccag taaagaagaa ctgaaaatga aaataaaagt
     5701 ttcttcttca gaaagacaca gctcttctga tgaaggcagt gggaagagca aacattcaag
     5761 cccacatatt agcagagacc ataaggagaa gcacaaggag catccttcaa gccgccacca
     5821 caccagcagc cacaagcatt cccactcgca tagtggcagc agcagcggtg gcagtaaaca
     5881 cagtgccgac ggaataccac ccactgttct gaggagtcct gttggcctga gcagtgatgg
     5941 catttcctct agctccagct cttcaaggaa gaggctgcat gtcaatgatg catctcacaa
     6001 ccaccactcc aaaatgagca aaagttccaa aagttcaggt agttcatcta gttcttcctc
     6061 ctctgttaag cagtatatat cctctcacaa ctctgttttt aaccatccct taccccctcc
     6121 tccccctgtc acataccagg tgggctacgg acatctcagc accctcgtga aactggacaa
     6181 gaagccagtg gagaccaacg gtcctgatgc caatcacgag tacagtacaa gcagccagca
     6241 tatggactac aaagacacat tcgacatgct ggactcactg ttaagtgccc aaggaatgaa
     6301 catgtagtaa tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc
     6361 ttgtggaaag gacgatcctg cctgtacatg cctatgtata cgcgttaagt cgacaatcaa
     6421 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     6481 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     6541 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     6601 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     6661 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     6721 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     6781 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     6841 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     6901 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     6961 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     7021 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     7081 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     7141 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     7201 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     7261 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta
     7321 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     7381 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     7441 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     7501 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     7561 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     7621 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     7681 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     7741 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     7801 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     7861 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     7921 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     7981 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     8041 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     8101 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     8161 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     8221 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     8281 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     8341 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     8401 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     8461 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     8521 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     8581 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     8641 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     8701 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     8761 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     8821 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     8881 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     8941 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     9001 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     9061 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     9121 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     9181 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     9241 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     9301 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     9361 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     9421 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     9481 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     9541 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     9601 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     9661 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     9721 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     9781 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     9841 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     9901 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     9961 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    10021 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    10081 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    10141 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    10201 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    10261 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    10321 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
    10381 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
    10441 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
    10501 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagc
//
