LOCUS       sequence-404102-       13045 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13045)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2776
FEATURES             Location/Qualifiers
     source          1..13045
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    5111..5122
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     misc_feature    8902..9490
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8955..8975)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9373..9384)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9562..9795
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9873..9994
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9910..9929)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9964..9983
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10034..10169
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10189..10208)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10190..10208)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10218..10235)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10218..10234)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10227..10249)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10376..10831
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10463..10482)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10673..10694
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10857..10961
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10962..11030,11031..11822)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11180..11199)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11993..12581
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12482..12501
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12735..12752
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12869..12890
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12905..12910,12911..12928,12929..12935)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12943..12959
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12948..12970
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12967..12983
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12967..12983
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13002..13022
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13004..13022
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggag ccggggtccg acgatttcct
     4141 accgccaccg gagtgcccgg tgttcgagcc tagctgggcc gagttccgag accctcttgg
     4201 ctacatcgcg aaaatcaggc ccatcgcaga gaaatcgggc atttgcaaga tccgcccacc
     4261 cgcggactgg cagccaccct ttgctgtgga agtggacaac ttcaggttta ccccccgaat
     4321 ccagaggctg aatgagctag aggcccagac gagagtgaaa ctgaactact tggaccagat
     4381 tgccaaattc tgggaaatcc agggctcctc cttaaagatt cccaatgtag aacggcggat
     4441 cttggacctc tacagtctca gcaaaattgt ggtggaggaa ggtggttatg aagctatctg
     4501 caaggaccgt cggtgggctc gggtagccca gcgcctcaac tatccaccag gcaaaaatat
     4561 tggctccttg ctacgctccc actacgaacg cattgtttat ccctatgaaa tgtaccagtc
     4621 tggagccaac cttgtgtgta acacacgtcc atttgataat gaggagaagg acaaggaata
     4681 caaaccccac agcatccccc tacgacagtc tgtgcagcct tccaagttca acagctatgg
     4741 ccggcgggcc aagagactgc agcctgatcc ggaacccaca gaggaagaca ttgagaagaa
     4801 tccagagctg aaaaagctac agatctatgg ggcaggcccc aagatgatgg gcctgggcct
     4861 catggccaaa gacaagactc tgcggaagaa agataaggag gggcctgagt gtccccccac
     4921 agtagtggtg aaggaggagt taggtgggga tgtgaaggtg gagtcaacat cgcctaagac
     4981 cttcctggag agcaaggagg agctgagtca cagcccagaa ccctgcacca agatgaccat
     5041 gaggctacgg aggaaccaca gcaatgccca gtttattgag tcatatgtct gccggatgtg
     5101 ttctcgaggg gatgaggatg acaagctcct gctgtgtgat ggctgtgatg acaactacca
     5161 catcttctgc ctgctgcctc ctctgcctga gatccccaag ggtgtctggc ggtgcccaaa
     5221 gtgtgtcatg gcggagtgta agcggccccc agaagccttt ggctttgagc aggctacccg
     5281 ggaatacact ctgcagagct ttggcgagat ggccgactcc tttaaagctg actacttcaa
     5341 catgcccgtg catatggtgc ccacagaact tgtggagaag gagttctgga ggctggtaaa
     5401 tagcattgag gaagatgtga ctgttgagta tggagctgac atccattcca aagaatttgg
     5461 cagcggtttc cctgtcagtg acagtaaacg gcacctaacc cccgaagagg aggagtatgc
     5521 taccagtggt tggaacctaa atgtgatgcc ggtgttggaa cagtctgtac tgtgccacat
     5581 caatgcagat atctctggca tgaaggtgcc ctggctctac gtgggcatgg tcttctcagc
     5641 cttttgctgg catattgagg atcactggag ttactccatt aactacctcc actggggtga
     5701 gccgaagacc tggtatgggg tgccctcact tgcagcagaa catttggaag aagtgatgaa
     5761 gaagctgaca cctgaactat ttgatagcca gcctgacctc ctgcaccaac ttgtcaccct
     5821 catgaatccc aacaccctca tgtcccatgg tgtgccagtt gtccgcacaa accagtgtgc
     5881 aggagagttt gtcatcacct tcccccgtgc ttaccacagc ggcttcaacc aaggctacaa
     5941 ctttgccgag gctgtcaact tttgcactgc tgactggttg cctgctgggc gccagtgcat
     6001 tgagcactac cgccggctcc ggagatactg cgtcttctcc catgaggagc ttatctgcaa
     6061 gatggctgcc tgcccagaga agctagacct gaacctggcg gcagctgtgc ataaggagat
     6121 gttcatcatg gtgcaagaag agcggcgtct acgaaaggcc ctgctggaga agggtatcac
     6181 agaggctgag cgagaggctt tcgagctgct cccagatgat gagcgccagt gtatcaagtg
     6241 caagactacg tgtttcctgt cagccctggc ctgctacgac tgcccagacg gccttgtctg
     6301 cctttcccac atcaatgatc tctgcaagtg ctccagtagc cggcagtacc tgcggtatcg
     6361 gtataccttg gatgagcttc ctgccatgct gcataagctg aaggttcggg ctgagtcctt
     6421 tgacacctgg gccaacaaag tgcgagtggc cctggaggtg gaggatgggc ggaagcgcag
     6481 ccttgaagaa ctgagggcac tagagtctga agcccgtgag cggaggtttc ctaatagtga
     6541 gctgctgcag caactaaaga actgcctgag tgaggcagag gcttgcgtgt cccgagctct
     6601 gggactggtc agcggccagg aagctggccc ccacagggtg gctggtctac agatgaccct
     6661 gactgagctc cgggcctttc tggaccagat gaacaacctg ccttgcgcca tgcaccagat
     6721 tggggatgtc aagggtgttc tggaacaggt ggaggcctac caggctgagg ctcgtgaggc
     6781 cctggcctca ctgccctcca gtccagggct actgcagtcc ctgttggaga gggggcggca
     6841 gctgggggtg gaggtgcctg aggcccagca gctccagcgg caggtggaac aggcgcgatg
     6901 gctggatgag gtgaaacgca cactggcccc ctcagcccga aggggcacct tggctgtcat
     6961 gcgaggactg ttggtcgcgg gtgccagtgt agcccctagc cctgctgtgg ataaagccca
     7021 ggccgagctg caggaactgc tgaccattgc tgaacgctgg gaggagaaag cccacctctg
     7081 cctggaggcc aggcagaagc atccaccagc cacacttgag gccataatcc gtgaagcgga
     7141 aaacatccct gttcacctgc ccaacatcca ggctctcaag gaggctcttg ctaaggcccg
     7201 ggcctggatt gctgatgttg atgagatcca aaatggtgac cactacccct gcctggatga
     7261 cttggagggc ctagtagctg tgggccggga cctacctgtg gggctggagg agctgagaca
     7321 gctagagcta caggtactga cagcgcactc ctggagggag aaggcctcca agaccttcct
     7381 caagaaaaat tcttgctaca cgctgctgga ggttctctgc ccatgtgcag atgccggctc
     7441 agacagcacc aagcgcagcc ggtggatgga gaaggagctg gggttgtaca aatctgacac
     7501 agagctgctg gggctgtctg cgcaggacct cagggaccca ggctctgtga tcgtggcctt
     7561 caaggagggg gaacagaagg agaaggaggg tatcctgcag ctgcgtcgca ccaattcggc
     7621 caagcccagt ccactggcat catcgagcac ggcctcctct acaacctcta tctgtgtgtg
     7681 tgggcaggtg ctggctgggg cgggagctct gcagtgtgac ctgtgtcagg actggttcca
     7741 tgggcggtgt gtgtcagtgc ctcgcctcct cagctctccg aggcccaatc ccacctcatc
     7801 cccactgctg gcctggtggg aatgggacac caaattcctg tgtccactgt gtatgcgctc
     7861 aaggcgcccg cgcctggaga ccatcctggc actgctggta gccctgcaga gactgcctgt
     7921 gcggctgccc gagggcgagg ccctgcagtg cctcacagag agggccatca gctggcaagg
     7981 ccgcgccagg caggctctgg cctctgaaga tgtgactgct cttttgggac ggctggctga
     8041 gctccgccaa cggctacagg ctgaacctag acctgaggag cctcctaact accctgcagc
     8101 ccctgcttct gaccccctca gagagggcag tggcaaggat atgcctaagg tccagggctt
     8161 actggagaat ggagacagtg tgaccagtcc tgagaaggta gccccggagg agggctcagg
     8221 taagagagat ctggagctgc tgtcctcgct gttgccacag ttgactggcc ctgtgttgga
     8281 actgcctgag gcaacccggg cccccttgga ggagctcatg atggaggggg acctgctcga
     8341 ggtgaccctg gatgagaacc acagcatatg gcagctgctg caggctggac agcccccaga
     8401 cctggagagg atccgcacac ttctggagct ggagaaggca gagcgtcacg ggagtcgggc
     8461 tcggggccgg gccctggaga ggcggcggcg gcggaaggtg gatcggggtg gggagggcga
     8521 tgacccagcc cgagaggagc tagagccaaa gagggtacgg agctcagggc cagaggctga
     8581 ggaggtccag gaggaggaag agctggagga ggagactggg ggtgagggcc cccctgcacc
     8641 catccccacc actggcagcc ccagcaccca ggagaaccag aatggcttgg aaccggcgga
     8701 agggaccact tcaggcccct cggccccttt ctccactctg actccccggc tgcatctgcc
     8761 ctgcccacag cagccgcctc agcaacagtt gtagtaatga actagtccgt aacttgaaag
     8821 tatttcgatt tcttggcttt atatatcttg tggaaaggac gataaggtgt agtcttaacg
     8881 taacggacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     8941 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     9001 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     9061 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     9121 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     9181 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     9241 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     9301 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     9361 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     9421 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     9481 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
     9541 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     9601 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     9661 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     9721 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     9781 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
     9841 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     9901 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     9961 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
    10021 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
    10081 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
    10141 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
    10201 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
    10261 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
    10321 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
    10381 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
    10441 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
    10501 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
    10561 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
    10621 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
    10681 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
    10741 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
    10801 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
    10861 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
    10921 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
    10981 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
    11041 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
    11101 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
    11161 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
    11221 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
    11281 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
    11341 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
    11401 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
    11461 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
    11521 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
    11581 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
    11641 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
    11701 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
    11761 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
    11821 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
    11881 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
    11941 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    12001 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    12061 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    12121 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    12181 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    12241 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    12301 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    12361 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    12421 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    12481 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    12541 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    12601 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    12661 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    12721 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
    12781 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
    12841 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
    12901 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
    12961 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
    13021 gggaacaaaa gctggagctg caagc
//
