LOCUS       sequence-404112-       13108 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13108)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2772
FEATURES             Location/Qualifiers
     source          1..13108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4445..4456
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     misc_feature    8965..9553
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9018..9038)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9436..9447)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9625..9858
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9936..10057
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9973..9992)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10027..10046
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10097..10232
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10252..10271)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10253..10271)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10281..10298)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10281..10297)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10290..10312)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10439..10894
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10526..10545)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10736..10757
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10920..11024
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11025..11093,11094..11885)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11243..11262)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12056..12644
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12545..12564
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12798..12815
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12932..12953
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12968..12973,12974..12991,12992..12998)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13006..13022
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13011..13033
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13030..13046
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13030..13046
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13065..13085
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13067..13085
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggag gcggccacca cactgcaccc
     4141 aggcccgcgc ccggcgctgc ccctcggggg cccgggcccg ctgggcgagt tcctgcctcc
     4201 acccgagtgc ccggtcttcg aacccagctg ggaagagttc gcggacccct tcgctttcat
     4261 ccacaagatc cggcccatag ccgagcagac tggcatctgt aaggtgcggc cgccgccgga
     4321 ttggcagcca ccatttgcat gtgatgttga taaacttcat tttacgccac gtatccagag
     4381 actgaatgaa ttggaggccc aaactcgtgt aaaattgaat ttcttggacc agattgcaaa
     4441 gtactgggag ttacagggaa gtactctgaa aattccacat gtggagagga agatcttgga
     4501 cttatttcag cttaataagt tagttgcaga agaaggtgga tttgcagttg tttgcaagga
     4561 tagaaaatgg accaaaattg ctaccaagat ggggtttgct cctggcaaag cagtgggctc
     4621 acatatcaga gggcattatg aacgaattct caacccctac aacttattcc tgtccggaga
     4681 cagcctaagg tgtttgcaga agccaaacct gaccacagac actaaggaca aggagtacaa
     4741 accccatgat attccccaga ggcagtctgt gcagccttcg gaaacgtgcc ccccagcccg
     4801 acgagcaaaa cgcatgagag cagagagaca gagtctcgct gtgttgccca ggctggagtg
     4861 cagtggcgcg atcttggctc actgcaacct ccgcctccta gattccagca attcttctgc
     4921 ctcagcctcc caagccatga atattaaaat agaacccgag gagacaacgg aagccagaac
     4981 tcataatctg agacgtcgaa tgggttgtcc aactccaaaa tgtgaaaatg agaaagaaat
     5041 gaagagtagc atcaagcaag aacctattga gaggaaagat tatattgtag aaaatgagaa
     5101 ggaaaagccc aagagtcgat ctaaaaaagc caccaatgct gtggacctgt atgtctgtct
     5161 tttatgtggc agtggcaatg atgaagaccg gctactgttg tgtgatggct gtgatgacag
     5221 ttaccatacc ttttgcttga tcccacctct ccatgatgtt cccaagggag actggaggtg
     5281 tcctaagtgt ttggctcagg aatgtagtaa gccacaagaa gcatttggct ttgaacaagc
     5341 agccagggac tataccctcc gtacttttgg ggaaatggca gatgcgttca aatctgatta
     5401 cttcaacatg ccagtccata tggtccccac agagcttgtt gagaaagaat tttggagact
     5461 agtaagcact attgaggagg atgtcacagt ggaatatgga gctgacattg cctcaaagga
     5521 atttggcagt ggctttcctg tccgagatgg gaaaatcaaa ctctcacctg aggaagagga
     5581 gtatcttgat agtggctgga atttgaacaa catgccagtg atggagcagt ctgtccttgc
     5641 acatattact gctgatatat gtggcatgaa acttccttgg ttgtatgtgg gaatgtgctt
     5701 ttcttcattc tgttggcaca ttgaagacca ctggagctat tcaattaact acttgcactg
     5761 gggtgagcca aaaacctggt atggagtccc agggtatgct gctgagcagc tagaaaatgt
     5821 aatgaagaaa ctagctccag aactctttgt gtcccagccg gatctcctcc atcagcttgt
     5881 gaccatcatg aaccccaata ccctgatgac tcatgaagtg cctgtttacc gaactaatca
     5941 gtgtgctggg gagtttgtga ttacatttcc aagagcctac cacagtggtt ttaaccaggg
     6001 ttttaatttt gctgaggctg ttaacttctg cactgttgat tggctgccat taggccgaca
     6061 gtgtgtggag cattatcgct tgcttcatcg atattgtgtg ttttcccacg atgagatgat
     6121 ctgcaagatg gcttccaagg ctgatgtatt agatgttgta gtggcttcaa ctgttcagaa
     6181 agacatggcc attatgattg aggatgagaa agctttaaga gaaactgtcc gtaaattggg
     6241 agtgattgat tcggaaagaa tggattttga gctgttgcca gatgatgaac gtcagtgtgt
     6301 aaaatgcaaa actacatgct tcatgtctgc catctcctgt tcttgtaaac ctggccttct
     6361 tgtttgcctg catcatgtaa aagaattgtg ttcctgtcct ccttacaaat ataaattgcg
     6421 gtataggtac acgctggatg atctctaccc tatgatgaat gcattgaagc ttcgagcaga
     6481 atcttacaac gaatgggcct tgaatgtgaa tgaagctttg gaggcaaaga tcaacaagaa
     6541 gaaaagcctt gtcagcttca aggctttaat tgaagaatct gaaatgaaga aattcccaga
     6601 caatgatctt ttgcgacacc ttcgcctagt cacacaggat gcagagaagt gtgcctctgt
     6661 tgcgcagcag ttgcttaatg gcaaaaggca aactagatat cgatctggtg gagggaaatc
     6721 ccaaaatcag ttgacagtga atgagctccg gcagtttgta acacagctgt atgctcttcc
     6781 atgtgtcctc agtcagacac cattactaaa ggatctcttg aatcgtgtag aagattttca
     6841 acagcatagt cagaaactac tctctgagga aacgcctagt gctgcggagc tgcaggactt
     6901 gctagatgtc agctttgaat ttgatgttga acttccacag cttgctgaga tgcgtatccg
     6961 tttggaacaa gcccgttggc tagaagaggt gcagcaagct tgcctagacc ccagctccct
     7021 tactttagat gatatgagac gtctcataga cctaggggta gggctggccc cgtattcagc
     7081 agtggagaaa gctatggccc ggctgcagga actgctcaca gtgtcagagc actgggacga
     7141 caaagccaag agtctcctca aggccaggcc acgacattca ttgaatagcc ttgctacggc
     7201 agtaaaggaa atcgaagaga tccctgcata tctgcccaat ggtgcggctc tgaaagactc
     7261 agtgcagaga gccagagact ggcttcagga tgtagagggc ctgcaggctg gaggacgtgt
     7321 gccagtgtta gacacactca tagaacttgt tacacgaggc cgatctatcc ccgtacatct
     7381 gaattctttg ccaagactgg aaaccctagt agctgaggtt caggcttgga aagaatgtgc
     7441 tgttaataca ttcttgactg agaattctcc atattctctc ttagaggtgc tgtgtcctcg
     7501 atgtgatatt ggccttttgg gattgaaaag gaagcagaga aagttaaagg agcccttgcc
     7561 aaatggaaag aaaaaaagca ccaaattaga gagtctgagt gacctggaga gagctttaac
     7621 tgaaagcaag gagactgctt cagctatggc aactcttggg gaagctcgcc taagggaaat
     7681 ggaagccttg cagtctctca gactcgccaa tgaagggaaa ttgctgtcgc ctctccaaga
     7741 tgtggatata aaaatctgcc tatgtcagaa ggccccagct gcccctatga ttcaatgtga
     7801 actctgcagg gatgctttcc acaccagttg tgtggcggta cccagtattt cacagggcct
     7861 gcgaatctgg ctttgtcccc attgtcggag gtcagagaaa cctccattag agaaaattct
     7921 gcccctgctc gcctcccttc agcgtatccg agttcgcctt cctgagggag atgcacttcg
     7981 atatatgatt gaaagaaccg tgaactggca gcacagagcc cagcaactgc tttcgtcagg
     8041 gaatcttaaa tttgtgcaag atcgagtggg ctcaggactg ttatatagca gatggcaagc
     8101 ctcagcagga caggtgtcag acacaaacaa ggtatctcaa cctcctggca caacatcatt
     8161 ttctttgcct gatgactggg acaacagaac ctcatatttg cactccccct tctcaactgg
     8221 acgaagttgt atccccctcc atggtgttag tccagaagtg aatgaactat tgatggaagc
     8281 ccagctgctc caggtatccc ttcctgaaat tcaggaactt taccagactt tacttgcaaa
     8341 gccaagccct gctcagcaga ctgaccgaag ctcaccagtg agacccagca gtgagaagaa
     8401 tgactgttgc cgagggaagc gagatggaat taacagtctt gagagaaaac tgaagagacg
     8461 cctggaaaga gagggcctct ccagtgagcg gtgggaacga gttaagaaaa tgcggacccc
     8521 caaaaagaag aaaatcaaac tgagccaccc caaggacatg aacaatttca agttagagag
     8581 agagcgtagc tatgaattag ttcgttctgc tgaaactcat tccctgccct cagacacatc
     8641 ctattccgaa caggaagact ctgaggatga agatgccatc tgcccagctg tgagctgcct
     8701 gcagccagaa ggagatgagg tggactgggt ccagtgtgat ggcagctgca atcagtggtt
     8761 tcatcaggtc tgtgttggtg tctccccaga gatggcagag aaagaagact acatctgtgt
     8821 gcgctgtact gtgaaggacg caccaagccg aaagtagtaa tgaactagtc cgtaacttga
     8881 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgagcacg tatacgataa
     8941 cactgccgaa cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     9001 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     9061 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     9121 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     9181 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     9241 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     9301 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     9361 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     9421 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     9481 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     9541 gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     9601 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     9661 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     9721 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     9781 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     9841 tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat
     9901 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     9961 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    10021 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
    10081 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
    10141 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
    10201 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
    10261 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
    10321 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
    10381 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
    10441 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
    10501 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
    10561 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
    10621 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
    10681 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
    10741 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
    10801 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
    10861 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
    10921 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
    10981 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
    11041 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
    11101 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
    11161 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
    11221 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
    11281 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
    11341 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
    11401 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
    11461 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
    11521 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
    11581 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
    11641 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
    11701 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
    11761 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
    11821 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    11881 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    11941 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    12001 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    12061 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    12121 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    12181 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
    12241 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    12301 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    12361 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    12421 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    12481 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    12541 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    12601 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    12661 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    12721 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
    12781 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
    12841 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
    12901 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
    12961 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
    13021 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
    13081 aaagggaaca aaagctggag ctgcaagc
//
