LOCUS       sequence-404123-       13126 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13126)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2885
FEATURES             Location/Qualifiers
     source          1..13126
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8983..9571
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9036..9056)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9454..9465)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9643..9876
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9954..10075
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9991..10010)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10045..10064
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10115..10250
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10270..10289)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10271..10289)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10299..10316)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10299..10315)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10308..10330)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10457..10912
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10544..10563)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10754..10775
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10938..11042
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11043..11111,11112..11903)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11261..11280)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12074..12662
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12563..12582
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12816..12833
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12950..12971
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12986..12991,12992..13009,13010..13016)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13024..13040
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13029..13051
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13048..13064
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13048..13064
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13083..13103
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13085..13103
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggag acgtctgcct cagccactgc
     4141 ctccgagaag caagaagcca aaagtgggat cctggaggcc gctggcttcc ccgacccggg
     4201 taaaaaggcc tctcctttgg tggtggctgc agcggcagca gcagcggtag ctgcccaagg
     4261 agtgccgcag catctcttgc caccattcca tgcgccccta ccgattgaca tgcgacacca
     4321 ggaaggaagg taccattacg agcctcattc tgtccacggt gtgcacgggc cccctgccct
     4381 cagcggcagc cctgtcatct ctgacatctc cttgatccgg ctttccccgc acccggctgg
     4441 ccctggggag tcccccttca acgcccccca cccgtacgtg aacccccaca tggagcacta
     4501 cctccgttct gtgcacagca gccccacgct ctccatgatc tctgcagcca ggggcctcag
     4561 ccccgctgat gtggcccagg agcaccttaa ggagagggga ctgtttggcc ttcctgctcc
     4621 aggcaccacc ccctcagact attaccacca gatgaccctc gtggcaggcc accccgcgcc
     4681 ctacggggac ctgctgatgc agagcggggg cgctgccagc gcaccccatc tccacgacta
     4741 cctcaacccc gtggacgtgt cccgtttctc cagcccgcgg gtgacgcccc gcctgagccg
     4801 caagcgggcg ctgtccatct ccccactctc agacgccagc ctggacctgc agcggatgat
     4861 ccgcacctca cccaactcgc tagtggccta catcaacaac tcccgaagca gctcagcggc
     4921 cagcggttcc tacgggcatc tgtcagcggg tgccctcagc ccagccttca ccttccccca
     4981 ccccatcaac cccgtggcct accagcagat tctgagccag cagaggggtc tggggtcagc
     5041 ctttggacac acaccacccc tgatccagcc ctcacccacc ttcctggccc agcagcccat
     5101 ggccctcacc tccatcaatg ccacgcccac ccagctcagc agcagcagca actgtctgag
     5161 tgacaccaac cagaacaagc agagcagtga gtcggccgtc agcagcaccg tcaaccctgt
     5221 cgccattcac aagcgcagca aggtcaagac cgagcctgag ggcctgcggc cggcctcccc
     5281 tctggcgctg acgcagggcc aggtgtctgg acacggctca tgtgggtgtg cccttcccct
     5341 ctcccaggag cagctggctg acctcaagga agatctggac agggatgact gtaagcagga
     5401 ggctgaggtg gtcatctatg agaccaactg ccactgggaa gactgcacca aggagtacga
     5461 cacccaggag cagctggtgc atcacatcaa caacgagcac atccacgggg agaagaagga
     5521 gtttgtgtgc cgctggcagg cctgcacgcg ggagcagaag cccttcaagg cgcagtacat
     5581 gctggtggtg cacatgcggc gacacacggg cgagaagccc cacaagtgca cgttcgaggg
     5641 ctgctcgaag gcctactccc gcctggagaa cctgaagaca cacctgcggt cccacaccgg
     5701 ggagaagcca tatgtgtgtg agcacgaggg ctgcaacaaa gccttctcca acgcctcgga
     5761 ccgcgccaag caccagaatc gcacccactc caacgagaaa ccctacatct gcaagatccc
     5821 aggctgcacc aagagataca cagaccccag ctctctccgg aagcatgtga aaacggtcca
     5881 cggcccagat gcccacgtca ccaagaagca gcgcaatgac gtgcacctcc gcacaccgct
     5941 gctcaaagag aatggggaca gtgaggccgg cacggagcct ggcggcccag agagcaccga
     6001 ggccagcagc accagccagg ccgtggagga ctgcctgcac gtcagagcca tcaagaccga
     6061 gagctccggg ctgtgtcagt ccagccccgg ggcccagtcg tcctgcagca gcgagccctc
     6121 tcctctgggc agtgccccca acaatgacag tggcgtggag atgccgggga cggggcccgg
     6181 gagcctggga gacctgacgg cactggatga cacaccccca ggggccgaca cctcagccct
     6241 ggctgccccc tccgctggtg gcctccagct gcgcaaacac atgaccacca tgcaccggtt
     6301 cgagcagctc aagaaggaga agctcaagtc actcaaggat tcctgctcat gggccgggcc
     6361 gactccacac acgcggaaca ccaagctgcc tcccctcccg ggaagtggct ccatcctgga
     6421 aaacttcagt ggcagtgggg gcggcgggcc cgcggggctg ctgccgaacc cgcggctgtc
     6481 ggagctgtcc gcgagcgagg tgaccatgct gagccagctg caggagcgcc gcgacagctc
     6541 caccagcacg gtcagctcgg cctacaccgt gagccgccgc tcctccggca tctcccccta
     6601 cttctccagc cgccgctcca gcgaggcctc gcccctgggc gccggccgcc cgcacaacgc
     6661 gagctccgct gactcctacg accccatctc cacggacgcg tcgcggcgct cgagcgaggc
     6721 cagccagtgc agcggcggct ccgggctgct caacctcacg ccggcgcagc agtacagcct
     6781 gcgggccaag tacgcggcag ccactggcgg ccccccgccc actccgctgc cgggcctgga
     6841 gcgcatgagc ctgcggacca ggctggcgct gctggacgcg cccgagcgca cgctgcccgc
     6901 cggctgccca cgcccactgg ggccgcggcg tggcagcgac gggccgacct atggccacgg
     6961 ccacgcgggg gctgcgcccg ccttccccca cgaggctcca ggcggcggag ccaggcgggc
     7021 cagcgaccct gtgcggcggc ccgatgccct gtccctgccg cgggtgcagc gcttccacag
     7081 cacccacaac gtgaaccccg gcccgctgcc gccctgtgcc gacaggcgag gcctccgcct
     7141 gcagagccac ccgagcaccg acggcggcct ggcccgcggc gcctactcgc cccggccgcc
     7201 tagcatcagc gagaacgtgg cgatggaggc cgtggcggca ggagtggacg gcgcggggcc
     7261 cgaggccgac ctggggctgc cggaggacga cctggtgctt ccagacgacg tggtgcagta
     7321 catcaaggcg cacgccagtg gcgctctgga cgagggcacc gggcaggtgt atcccacgga
     7381 aagcactggc ttctctgaca accccagact acccagcccg gggctgcacg gccagcgcag
     7441 gatggtggct gcggactcca acgtgggccc ctccgcccct atgctgggag gatgccagtt
     7501 aggctttggg gcgccctcca gcctgaacaa aaataacatg cctgtgcagt ggaatgaggt
     7561 gagctccggc accgtagacg ccctggccag ccaggtgaag cctccaccct ttcctcaggg
     7621 caacctggcg gtggtgcagc agaagcctgc ctttggccag tacccgggct acagtccgca
     7681 aggcctacag gctagccctg ggggcctgga cagcacgcag ccacacctgc agccccgcag
     7741 cggagccccc tcccagggca tccccagggt aaactacatg cagcagctgc gacagccagt
     7801 ggcaggcagc cagtgtcctg gcatgactac cactatgagc ccccatgcct gctatggcca
     7861 agtccacccc cagctgagcc ccagcaccat cagtggggcc ctcaaccagt tcccccaatc
     7921 ctgcagcaac atgccagcca agccagggca tctggggcac cctcagcaga cagaagtggc
     7981 acctgacccc accacgatgg gcaatcgcca cagggaactt ggggtccccg attcagccct
     8041 ggctggagtg ccaccacctc acccagtcca gagctaccca cagcagagcc atcacctggc
     8101 agcctccatg agccaggagg gctaccacca ggtccccagc cttctgcctg cccgccagcc
     8161 tggcttcatg gagccccaaa caggcccgat gggggtggct acagcaggct ttggcctagt
     8221 gcagccccgg cctcccctcg agcccagccc cactggccgc caccgtgggg tacgtgctgt
     8281 gcagcagcag ctggcctacg ccagggccac aggccatgcc atggctgcca tgccgtccag
     8341 tcaggaaaca gcagaggctg tgcccaaggg agcgatgggc aacatggggt cggtgcctcc
     8401 ccagccgcct ccgcaggacg caggtggggc cccggaccac agcatgctct actactacgg
     8461 ccagatccac atgtacgaac aggatggagg cctggagaac ctcgggagct gccaggtcat
     8521 gcggtcccag ccaccacagc cacaggcctg tcaggacagc atccagcccc agcccttgcc
     8581 ctcaccaggg gtcaaccagg tgtccagcac tgtggactcc cagctcctgg aggcccccca
     8641 gattgacttc gatgccatca tggatgatgg cgatcactcg agtttgttct cgggtgctct
     8701 gagccccagc ctcctccaca gcctctccca gaactcctcc cgcctcacca ccccccgaaa
     8761 ctccttgacc ctgccctcca tccccgcagg catcagcaac atggctgtcg gggacatgag
     8821 ctccatgctc accagcctcg ccgaggagag caagttcctg aacatgatga cctagtaatg
     8881 aactagtccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
     8941 cgattagtct ttgtactcac cttggcgacg cgttaagtcg acaatcaacc tctggattac
     9001 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     9061 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     9121 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     9181 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     9241 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     9301 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     9361 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     9421 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     9481 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     9541 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
     9601 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     9661 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     9721 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     9781 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     9841 tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc
     9901 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     9961 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
    10021 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
    10081 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
    10141 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
    10201 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
    10261 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
    10321 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
    10381 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
    10441 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
    10501 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
    10561 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
    10621 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
    10681 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
    10741 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
    10801 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
    10861 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
    10921 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
    10981 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
    11041 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
    11101 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
    11161 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
    11221 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
    11281 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
    11341 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
    11401 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
    11461 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
    11521 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
    11581 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
    11641 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
    11701 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
    11761 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
    11821 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
    11881 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
    11941 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    12001 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    12061 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    12121 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    12181 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
    12241 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    12301 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    12361 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    12421 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    12481 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    12541 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    12601 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    12661 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    12721 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
    12781 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
    12841 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
    12901 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
    12961 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
    13021 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
    13081 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagc
//
