LOCUS       sequence-404143-       12685 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12685)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1225
FEATURES             Location/Qualifiers
     source          1..12685
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8542..9130
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8595..8615)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9013..9024)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9202..9435
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9513..9634
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9550..9569)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9604..9623
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9674..9809
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9829..9848)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9830..9848)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9858..9875)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9858..9874)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9867..9889)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10016..10471
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10103..10122)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10313..10334
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10497..10601
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10602..10670,10671..11462)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10820..10839)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11633..12221
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12122..12141
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12375..12392
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12509..12530
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12545..12550,12551..12568,12569..12575)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12583..12599
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12588..12610
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12607..12623
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12607..12623
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12642..12662
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12644..12662
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggcc agctcggaga ctgagatccg
     4141 ctgggctgag cctggcctgg ggaagggccc ccagcggcgg cgctgggcct gggccgagga
     4201 caagagggat gtggatagaa gtagttcaca aagctgggaa gaagagagac tctttcccaa
     4261 tgccaccagc cccgagctcc tagaggactt ccgcctggcc cagcagcacc tgccgcccct
     4321 ggagtgggac ccacacccgc agcccgatgg gcatcaggat tccgagtcag gagagacttc
     4381 gggagaagag gctgaagcag aggatgtgga cagcccagca agttcccatg agcctcttgc
     4441 ctggctcccc cagcagggcc gtcagctgga catgactgaa gaggagccag atgggaccct
     4501 cggaagtctg gaggttgagg aggctggaga gagctcctca aggttggggt atgaggctgg
     4561 tctcagcttg gaaggccatg gaaacaccag ccccatggct cttgggcatg gtcaggccag
     4621 gggctgggtg gcttctggcg aacaagccag tggggacaaa ctttctgaac attccgaggt
     4681 caacccatcc gttgaactca gcccggcaag gtcctggagc agtgggacag tgagcctcga
     4741 ccaccctagt gacagccttg attctacctg ggaaggagag accgatggcc cccagcccac
     4801 tgccctggca gaaaccttgc cagagggccc cagccaccac ctcctaagcc cagatggcag
     4861 aactggaggc agtgttgctc gggcaacccc catggaattc caggactcct cagctccccc
     4921 agcccagagt ccgcagcatg ccacagatag atggaggaga gaaacgacca gattcttctg
     4981 ccctcagccc aaggaacaca tctggaagca gacaaagacg tcacctaagc cactcccttc
     5041 ccgattcatt ggctccatca gccccctgaa tccccagccc aggccaacgc ggcagggcag
     5101 gccgctgccc agacagggag ccactctggc tggccgctcc tcttctaatg cccccaagta
     5161 tggccggggg cagttgaact acccactccc tgatttctcc aaggtagggc cccgggtgag
     5221 attccccaaa gatgagagct accgtccccc caagtccaga agccacaaca ggaagcctca
     5281 ggcccctgcc aggcccctca tcttcaagtc tccagctgag attgtgcagg aggtgctgtt
     5341 gagcagtgga gaagcagccc tggcaaagga cacgcctcct gcccacccta tcaccagggt
     5401 accccaagaa tttcagacgc ctgagcaagc cactgagctg gtccatcagc tccaggaaga
     5461 ctaccacagg ctcctcacca agtacgctga ggccgagaac accattgacc agctacgcct
     5521 cggggccaag gtgaacctgt tctctgaccc accccagccc aaccacagca tccacacggg
     5581 aatggtgccc caggggacca aggtcttgtc cttcaccatc ccacagcccc gctctgcaga
     5641 gtggtggccg ggcccggccg aggaccccca ggcctctgcg gcctcagggt ggccatcagc
     5701 tcgaggagac ttgagcccct cctcgcttac cagcatgccc accctggggt ggcttccgga
     5761 gaaccgggac atctctgagg accagtcctc agcagagcag acccaggcac tggcttctca
     5821 ggccagccag ttcctggcca aggtggagtc ctttgaaaga ctgatacagg caggacgtct
     5881 catgccccag gaccaagtca agggcttcca gcggctgaag gctgcccacg cggccctaga
     5941 ggaggagtac ctgaaggctt gtcgggagca acaccctgcc cagccgcttg ccggctccaa
     6001 ggggacgcct ggaagatttg atcctcgcag ggagctggag gcagagatat accgtctggg
     6061 aagctgcctg gaagagctga aggaacacat agaccagacc cagcaagagc ctgagccgcc
     6121 cgggtcagac tcagctctgg acagcacccc agccctgccc tgcctccatc agccaacgca
     6181 cctgcctgct ccttctggac aagcccccat gccagccatc aagacctcct gccctgagcc
     6241 tgctaccacc actgccgccg ccagcactgg cccctgccca ttgcacgtaa atgtggaggt
     6301 gagctctggc aacagtgagg tggaggacag gccacaggac cccctggccc gactcaggca
     6361 caaggagctg cagatggagc aagtttacca tggcctcatg gagcggtacc tcagtgtgaa
     6421 gtctctccca gaagccatga gaatggagga ggaggaagaa ggagaggagg aggaggagga
     6481 agagggggga ggtgactccc tggaagttga tggggtggct gcaactccag ggaaagcaga
     6541 ggccaccagg gtcctcccaa ggcagtgccc ggtgcaggct gagaaaagtc atggggctcc
     6601 cctggaggag gccacggaga agatggtatc tatgaagcca ccaggtttcc aggcatccct
     6661 ggctagagac gggcacatgt caggcctggg caaggctgag gcagcccctc caggccctgg
     6721 cgtgccaccc caccctccag gcaccaagtc cgcagcatcc caccaaagta gtatgaccag
     6781 cctggaggga agcggcatct ctgagcgcct tccacagaag cctttgcacc gaggcggtgg
     6841 gccccacctg gaggagacct ggatggcgtc cccagagaca gacagtggct ttgtgggctc
     6901 agaaacaagc agagtttcac ccctcaccca gactccagag caccggctct cccacatcag
     6961 cacagcagga acattagccc agccctttgc tgcatctgtg cccagggatg gagcttccta
     7021 ccccaaggcc aggggttctc tgattcccag aagagccaca gagcccagca caccccggag
     7081 ccaagcacag aggtacctct ccagcccaag tgggcctctc cggcagaggg cacccaactt
     7141 cagcctggag cggacactgg cagccgagat ggcggttcct ggctcagagt ttgaggggca
     7201 caaacggatt tctgaacagc cccttcccaa caagacaatc agcccacccc cagcccccgc
     7261 ccctgccgct gcgcctctac cctgtggacc aacagagacc atccccagct tcctgctcac
     7321 cagggcaggg cgagaccagg ccatctgtga gctgcaagaa gaggtgtccc ggcttcgtct
     7381 gcggctggaa gacagcctgc accagccact ccagggcagc ccgacacgcc cagcatctgc
     7441 ctttgaccgc cccgcccgga cccgcggccg gccagcagac tccccagcca cctggggctc
     7501 ccattatggc agtaaatcca cagagagatt gcctggtgag cctagaggtg aagagcagat
     7561 tgtccctcca ggaaggcagc gagccaggtc ttcctcagtg cctcgggagg tgctccgact
     7621 gtccctgagt tcagaatctg agctgccctc cctaccactg ttctctgaga agagcaagac
     7681 caccaaggac agtccacagg cagctcggga tggaaagaga ggggtgggca gtgctggatg
     7741 gccagacagg gtcaccttcc ggggccaata cacaggccac gaataccatg ttctgtcccc
     7801 taaggcggtc ccaaaaggca atggcacagt ctcctgtccc cactgccggc ccattaggac
     7861 ccaggatgcg ggtggtgctg tcacagggga cccactggga ccgcctcccg ctgataccct
     7921 tcagtgtccc ctgtgtggtc aagttgggtc tcccccagag gcagatggtc caggctcagc
     7981 cacctctggg gcagagaagg ccaccacgag gagaaaagca tcttcaactc ccagccccaa
     8041 gcagaggagc aagcaggcgg ggtcgtcgcc acgcccaccc cccggactgt ggtatctggc
     8101 aacagcgccc ccagcaccag cccctccagc ctttgcctac atctcctcgg ttcccatcat
     8161 gccttatcca cctgccgctg tgtactatgc gcctgcagga cctacctcag cccaaccagc
     8221 tgccaagtgg ccgcccacag cctctccccc accagcccgg agacaccggc actccatcca
     8281 gctcgacctg ggcgacctag aggagctcaa caaggccctg agccgggccg tgcaggctgc
     8341 cgagagcgtc cgctctacca ccaggcagat gagaagctcg ctgtcagccg acctgcgcca
     8401 ggctcacagc ctgcggggct cctgcctctt ctagtaatga actagtccgt aacttgaaag
     8461 tatttcgatt tcttggcttt atatatcttg tggaaaggac gatgaccaat atcgaggttt
     8521 cgtaccacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     8581 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     8641 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     8701 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     8761 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     8821 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     8881 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     8941 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     9001 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     9061 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     9121 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
     9181 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     9241 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     9301 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     9361 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     9421 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
     9481 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     9541 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     9601 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     9661 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     9721 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     9781 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     9841 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     9901 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     9961 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
    10021 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
    10081 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
    10141 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
    10201 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
    10261 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
    10321 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
    10381 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
    10441 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
    10501 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
    10561 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
    10621 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
    10681 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
    10741 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
    10801 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
    10861 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
    10921 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
    10981 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
    11041 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
    11101 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
    11161 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
    11221 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
    11281 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
    11341 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
    11401 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
    11461 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
    11521 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
    11581 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    11641 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    11701 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    11761 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    11821 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    11881 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    11941 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    12001 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    12061 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    12121 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    12181 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    12241 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    12301 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    12361 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
    12421 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
    12481 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
    12541 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
    12601 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
    12661 gggaacaaaa gctggagctg caagc
//
