LOCUS       sequence-404157-       12301 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12301)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1234
FEATURES             Location/Qualifiers
     source          1..12301
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8158..8746
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8211..8231)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8629..8640)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8818..9051
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9129..9250
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9166..9185)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9220..9239
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9290..9425
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9445..9464)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9446..9464)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9474..9491)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9474..9490)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9483..9505)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9632..10087
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9719..9738)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9929..9950
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10113..10217
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10218..10286,10287..11078)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10436..10455)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11249..11837
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11738..11757
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11991..12008
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12125..12146
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12161..12166,12167..12184,12185..12191)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12199..12215
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12204..12226
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12223..12239
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12223..12239
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12258..12278
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12260..12278
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggat ctattcgact ttttcagaga
     4141 ctgggacttg gagcagcagt gtcactatga acaagaccgt agtgcactta aaaaaaggga
     4201 atgggagcgg aggaatcaag aagtccagca agaagacgat ctcttttctt caggctttga
     4261 tctttttggg gagccataca aggtagctga atatacaaac aaaggtgatg cacttgccaa
     4321 ccgagtccag aacacgcttg gaaactatga tgaaatgaag aatttgctaa ctaaccattc
     4381 taatcagaat cacctagtgg gaattccaaa gaattctgtg ccccagaatc ccaacaacaa
     4441 aaatgaacca agcttttttc cagaacaaaa gaacagaata attccacctc accaggataa
     4501 tacccatcct tcagcaccaa tgcctccacc ttctgttgtg atactgaatt caactctaat
     4561 acacagcaac agaaaatcaa aacctgagtg gtcacgtgat agtcataacc ctagcactgt
     4621 actggcaagc caggccagtg gtcagccaaa caagatgcag actttgacac aggaccagtc
     4681 tcaagccaaa ctggaagact tctttgtcta cccagctgaa cagccccaga ttggagaagt
     4741 tgaagagtca aacccatctg caaaggaaga cagtaaccct aattctagtg gagaagatgc
     4801 tttcaaagaa atctttcaat ccaattcacc ggaagaatct gaattcgccg tgcaagcgcc
     4861 tgggtctccc ctagtggctt cctctttatt agctcctagc agtggccttt cagttcaaaa
     4921 cttcccacca gggctttact gcaaaacaag catggggcag caaaagccaa ctgcatacgt
     4981 cagacccatg gatggccagg accaggcacc ggacatctca ccaacactga aaccttcaat
     5041 tgaatttgag aacagctttg ggaatctgtc atttggaaca ctcttggatg gaaaacccag
     5101 tgcagccagt tcaaagacta aactgccaaa gttcaccatc ctccaaacaa gtgaagtaag
     5161 ccttcccagt gatccaagct gtgttgaaga aatcttgcgg gagatgaccc attcctggcc
     5221 tactcctctc acttccatgc atactgctgg acactctgag cagagcacct tttccatccc
     5281 aggacaggaa tcgcagcatc tgaccccagg attcacctta caaaagtgga atgacccaac
     5341 caccagagct tctacaaagt cagtgtcttt caaatcgatg cttgaggatg acctgaagct
     5401 gagcagtgat gaagatgacc ttgagcctgt gaagaccttg accactcagt gcactgccac
     5461 tgagctctac caggctgttg aaaaggcaaa acctaggaat aatcctgtga acccaccctt
     5521 ggccactccc cagcccccac ctgcagtgca agccagcggg ggttctggca gctccagcga
     5581 atcggagagc agctctgagt cggattcaga cactgaaagt agcaccactg acagcgaatc
     5641 taatgaggca cctcgtgtgg caactccaga gcctgagcca ccctcaacca acaagtggca
     5701 actggataaa tggcttaaca aagtgacatc ccagaacaag tcttttattt gtggccaaaa
     5761 tgaaacaccc atggagacta tttctctgcc tcctccaatc atccaaccaa tggaagtcca
     5821 gatgaaagtg aagacgaatg ccagtcaggt cccagctgaa cccaaagaaa ggcctctcct
     5881 cagtctcatt agggagaaag cccgtccacg gcccactcag aaaattccag aaacaaaggc
     5941 tttgaagcat aagttgtcaa caactagtga gacagtgtct caaaggacaa ttgggaaaaa
     6001 acagcccaaa aaagttgaga agaacaccag cactgacgag tttacctggc ccaaaccaaa
     6061 tattaccagc agcactccca aagaaaaaga aagtgtggag cttcatgacc caccaagagg
     6121 ccgcaacaaa gccactgccc acaaaccagc ccctaggaaa gaaccaagac ctaacatccc
     6181 tttggctccc gagaagaaga agtacagagg gcctggcaag attgtgccaa agtctcggga
     6241 attcattgaa acagattcat ctacatctga ctccaacaca gatcaggaag agaccctgca
     6301 aatcaaagtc ctgcctccgt gcattatttc tggaggtaat actgccaaat ccaaggaaat
     6361 ctgtggtgcc agcctgaccc tcagcacctt aatgagtagc agtggcagca acaacaactt
     6421 atccatcagt aatgaagagc caacattttc acctattcct gtcatgcaaa ctgaaatcct
     6481 gtcccctctg cgagatcatg agaacctgaa aaacctctgg gtgaagattg accttgactt
     6541 actctctaga gtacctggcc acagctcact ccatgcagca cctgccaagc cagaccacaa
     6601 ggagactgcc acaaaaccca agcgtcagac agctgtcaca gctgtggaga aaccagcccc
     6661 taagggcaaa cgtaagcaca agccaataga agttgcagag aagatccctg agaagaagca
     6721 gcgcctggag gaggccacaa ctatctgctt gctccctcct tgcatctcac cagccccacc
     6781 ccacaagcct cccaacacta gagaaaataa ttcatccagg agagcaaata gaagaaagga
     6841 agaaaaacta tttcctcctc cactttcccc actgccagag gaccctccac gccgcagaaa
     6901 tgtcagtggc aataatggtc cctttggtca agacaaaaac atcgccatga ctggacaaat
     6961 cacatctacc aaacctaaga gaactgaagg caaattctgt gctactttca aagggatatc
     7021 ggtaaatgag ggagacactc caaaaaaggc atcctctgcc accatcactg tcaccaatac
     7081 tgctattgcc actgctactg tcactgctac tgccattgtc accaccactg tcacagctac
     7141 tgccaccgcc acggccacca ccacaactac taccactacc atttccacca tcacctctac
     7201 catcactact ggcctcatgg atagcagtca cctggagatg acgtcctggg cggctctgcc
     7261 ccttctatcc agcagcagca ctaatgtccg gagacccaag ctcacttttg atgactcggt
     7321 tcacaatgct gattattaca tgcaagaagc taagaagctg aagcacaaag ctgatgcact
     7381 gttcgagaaa tttggcaaag ctgtgaatta tgctgatgcc gccctctcct tcactgaatg
     7441 tggcaatgcc atggaacgcg accctctgga agcaaagtcc ccatacacca tgtactctga
     7501 gactgtggag ctcctcaggt atgcaatgag gctgaagaac tttgcaagtc ccttggcttc
     7561 ggatggggac aaaaagctag cagtactatg ctaccgatgt ttatcactcc tctatttgag
     7621 aatgtttaag ctgaagaagg accatgctat gaagtactcc agatcactga tggaatattt
     7681 taagcaaaat gcttcaaaag tcgcacagat accctctcca tgggtaagca atggaaagaa
     7741 cactccatcc ccagtgtctc tcaacaacgt ctcccccatc aacgcaatgg ggaactgtaa
     7801 caatggccca gtcaccattc cccagcgcat tcaccacatg gctgccagcc acgtcaacat
     7861 cactagcaat gtgttacggg gctatgaaca ctgggatatg gccgacaaac tgacaagaga
     7921 aaacaaagaa ttctttggtg atctggacac gctgatgggg cctctgaccc agcacagcag
     7981 catgaccaat cttgtccgct acgttcgcca aggactgtgt tggctgcgca tcgatgccca
     8041 cttgttgtag taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat
     8101 atcttgtgga aaggacgatg gctgtcataa gggaacaggt agacgcgtta agtcgacaat
     8161 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     8221 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     8281 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     8341 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     8401 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     8461 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     8521 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     8581 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     8641 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     8701 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac
     8761 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     8821 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     8881 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     8941 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     9001 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata
     9061 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     9121 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     9181 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     9241 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     9301 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     9361 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     9421 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     9481 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     9541 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     9601 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     9661 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     9721 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     9781 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     9841 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     9901 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     9961 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
    10021 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
    10081 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
    10141 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
    10201 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
    10261 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
    10321 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
    10381 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
    10441 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
    10501 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
    10561 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
    10621 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
    10681 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
    10741 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
    10801 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
    10861 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
    10921 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
    10981 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
    11041 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
    11101 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    11161 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    11221 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    11281 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    11341 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
    11401 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    11461 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    11521 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    11581 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    11641 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    11701 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    11761 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    11821 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    11881 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    11941 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    12001 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
    12061 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
    12121 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
    12181 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
    12241 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
    12301 c
//
