LOCUS       sequence-404159-       16270 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16270)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 16270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1892
FEATURES             Location/Qualifiers
     source          1..16270
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      10326..10335
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    12127..12715
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(12180..12200)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12598..12609)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             12787..13020
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    13098..13219
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13135..13154)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13189..13208
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      13259..13394
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(13414..13433)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(13415..13433)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(13443..13460)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13443..13459)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13452..13474)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      13601..14056
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13688..13707)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13898..13919
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        14082..14186
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(14187..14255,14256..15047)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(14405..14424)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      15218..15806
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15707..15726
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15960..15977
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    16094..16115
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(16130..16135,16136..16153,16154..16160)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16168..16184
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     16173..16195
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     16192..16208
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     16192..16208
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     16227..16247
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        16229..16247
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgtct ggcggcgccg cagagaagca
     4141 gagcagcact cccggttccc tgttcctctc gccgccggct cctgccccca agaatggctc
     4201 cagctccgat tcctccgtgg gggagaaact gggagccgcg gccgccgacg ctgtgaccgg
     4261 caggaccgag gagtacaggc gccgccgcca cactatggac aaggacagcc gtggggcggc
     4321 cgcgaccact accaccactg agcaccgctt cttccgccgg agcgtcatct gtgactccaa
     4381 tgccactgca ctggagcttc ccggccttcc tctttccctg ccccagccca gcatccccgc
     4441 ggctgtcccg cagagtgctc caccggagcc ccaccgggaa gagaccgtga ccgccaccgc
     4501 cacttcccag gtagcccagc agcctccagc cgctgccgcc cctggggaac aggccgtcgc
     4561 gggccctgcc ccctcgactg tccccagcag taccagcaaa gaccgcccag tgtcccagcc
     4621 tagccttgtg gggagcaaag aggagccgcc gccggcgaga agtggcagcg gcggcggcag
     4681 cgccaaggag ccacaggagg aacggagcca gcagcaggat gatatcgaag agctggagac
     4741 caaggccgtg ggaatgtcta acgatggccg ctttctcaag tttgacatcg aaatcggcag
     4801 aggctccttt aagacggtct acaaaggtct ggacactgaa accaccgtgg aagtcgcctg
     4861 gtgtgaactg caggatcgaa aattaacaaa gtctgagagg cagagattta aagaagaagc
     4921 tgaaatgtta aaaggtcttc agcatcccaa tattgttaga ttttatgatt cctgggaatc
     4981 cacagtaaaa ggaaagaagt gcattgtttt ggtgactgaa cttatgacgt ctggaacact
     5041 taaaacgtat ctgaaaaggt ttaaagtgat gaagatcaaa gttctaagaa gctggtgccg
     5101 tcagatcctt aaaggtcttc agtttcttca tactcgaact ccacctatca ttcaccgcga
     5161 tcttaaatgt gacaacatct ttatcaccgg ccctactggc tcagtcaaga ttggagacct
     5221 cggtctggca accctgaagc gggcttcttt tgccaagagt gtgataggta ccccagagtt
     5281 catggcccct gagatgtatg aggagaaata tgatgaatcc gttgacgttt atgcttttgg
     5341 gatgtgcatg cttgagatgg ctacatctga atatccttac tcggagtgcc aaaatgctgc
     5401 acagatctac cgtcgcgtga ccagtggggt gaagccagcc agttttgaca aagtagcaat
     5461 tcctgaagtg aaggaaatta ttgaaggatg catacgacaa aacaaagatg aaagatattc
     5521 catcaaagac cttttgaacc atgccttctt ccaagaggaa acaggagtac gggtagaatt
     5581 agcagaagaa gatgatggag aaaaaatagc cataaaatta tggctacgta ttgaagatat
     5641 taagaaatta aagggaaaat acaaagataa tgaagctatt gagttttctt ttgatttaga
     5701 gagagatgtc ccagaagatg ttgcacaaga aatggtagag tctgggtatg tctgtgaagg
     5761 tgatcacaag accatggcta aagctatcaa agacagagta tcattaatta agaggaaacg
     5821 agagcagcgg cagttggtac gggaggagca agaaaaaaaa aagcaggaag agagcagtct
     5881 caaacagcag gtagaacaat ccagtgcttc ccagacagga atcaagcagc tcccttctgc
     5941 tagcaccggc atacctactg cttctaccac ttcagcttca gtttctacac aagtagaacc
     6001 tgaagaacct gaggcagatc aacatcaaca actacagtac cagcaaccca gtatatctgt
     6061 gttatctgat gggacggttg acagtggtca gggatcctct gtcttcacag aatctcgagt
     6121 gagcagccaa cagacagttt catatggttc ccaacatgaa caggcacatt ctacaggcac
     6181 agtcccaggg catatacctt ctactgtcca agcacagtct cagccccatg gggtatatcc
     6241 accctcaagt gtgcctcgtc gtggccgtag catgtcggtt tgtgttccca tctttctgct
     6301 gttgcctctg tgtcccgcat ctctcccagt gctcttccac cccaccgcca gtactgtctg
     6361 cacctctttc tccttccctc ctccggactg ccccgaggaa acttttgccg aaaagctttc
     6421 taaagcattg gagagtgtcc tgcctatgca ctctgcctct cagcgcaagc accgacgctc
     6481 cagcctgcct tccctctttg tcagtactcc tcagtccatg gcgcatccgt gtggggggac
     6541 cccaacatac ccagaatcac agatattttt cccaactatt catgaacgtc cagtttcttt
     6601 ttcaccacct cccacctgcc caccgaaagt agccatttcc cagcggcgta agagcacctc
     6661 cttcctggaa gcccaaactc accacttcca acccctgctg aggactgttg gccaaagtct
     6721 tcttccacct ggtggcagcc caactaactg gacaccagag gccgtagtta tgttgggtac
     6781 tacagccagt agagtaactg gagagtcatg tgagatacag gtccatccta tgtttgaacc
     6841 atctcaagtt tacagtgact atagacctgg actagtactt ccagaagaag ctcactattt
     6901 tattcctcag gaagcagtgt atgtagctgg ggtacattac caggcccggg tggcagaaca
     6961 gtatgagggc attccataca actcatcagt actgtcaagt cctatgaaac agatacctga
     7021 acagaagcca gtacaagggg gccctacttc aagttctgtc tttgaatttc catctggaca
     7081 ggctttcctg gtaggacacc ttcagaatct aagattagat tctggattgg gtccgggatc
     7141 tcccctctct agtatttctg cacctatcag tacagatgct acacgtttga aatttcaccc
     7201 tgtctttgtt cctcattctg cgcctgctgt gttaactcat aacaatgaga gcagaagcaa
     7261 ctgtgtattt gaatttcatg ttcacacacc aagctcctct tcaggagaag gaggtggaat
     7321 tttacctcag cgtgtttacc gaaatcggca ggttgcagtg gacttgaatc aagaagaact
     7381 gcctcctcaa tcagttggat tacatggcta cttgcagcct gtgactgaag aaaagcataa
     7441 ttaccatgcc ccagaattga ccgtttctgt ggtagagcct atcggacaga actggccaat
     7501 aggaagccca gaatattcca gtgattcctc acaaatcact tcttcagacc ccagtgattt
     7561 tcagtcacct ccccctacag ggggagcagc tgcacctttt ggctctgacg tctcaatgcc
     7621 ctttatccat ctgcctcaga cagtgttaca agaatcccca cttttcttct gtttccccca
     7681 aggaaccaca tctcagcagg tcttaactgc ctcattttct tcaggaggat ctgcacttca
     7741 tccacaggca caggggcaga gccagggtca gccatcctca agtagcttaa caggggtttc
     7801 atcttcccaa cccatacaac atcctcagca gcagcaggga atacagcaga cagcccctcc
     7861 tcaacagaca gtgcagtatt cactttcaca gacatcaacc tccagtgagg ccactactgc
     7921 acagccagtg agtcagcctc aagctccaca agtcttgcct caagtatcag ctggaaaaca
     7981 gagtactcag ggagtctctc aggttgctcc tgcagagcca gttgcagtag cacagaccca
     8041 agctacccag ccgaccactt tggcttcctc tgtagacagt gcacattcag atgttgcttc
     8101 aggtatgagt gatggcaatg agaacgtccc atcttccagt ggaaggcatg aaggaagaac
     8161 tacaaaacgg cattaccgaa aatctgtaag gagtcgctct cgacatgaaa aaacttcacg
     8221 cccaaaatta agaattttga atgtttcaaa taaaggagac cgagtagtag aatgtcaatt
     8281 agagactcat aataggaaaa tggttacatt caaatttgac ctagatggtg acaaccccga
     8341 ggagatagca acaattatgg tgaacaatga ctttattcta gcaatagaga gagagtcgtt
     8401 tgtggatcaa gtgcgagaaa ttattgaaaa agctgatgaa atgctcagtg aggatgtcag
     8461 tgtggaacca gagggtgatc agggattgga gagtctacaa ggaaaggatg actatggctt
     8521 ttcaggttct cagaaattgg aaggagagtt caaacaacca attcctgcgt cttccatgcc
     8581 acagcaaata ggcattccta ccagttcttt aactcaagtt gttcattctg cgggaaggcg
     8641 gtttatagtg agtcctgtgc cagaaagccg attacgagaa tcaaaagttt tccccagtga
     8701 aataacagat acagttgctg cctctacagc tcagagccct ggaatgaact tgtctcactc
     8761 tgcatcatcc cttagtctac aacaggcctt ttctgaactt agacgtgccc aaatgacaga
     8821 aggacccaac acagcacctc caaactttag tcatacagga ccaacatttc cagtagtacc
     8881 tcctttctta agtagcattg ctggagtccc aaccacagca gcagccacag caccagtccc
     8941 tgcaacaagc agccctccta atgacatttc cacatcagta attcagtctg aggttacagt
     9001 gcccactgaa gaggggattg ctggagttgc caccagcaca ggtgtggtaa cttcaggtgg
     9061 tctccccata ccacctgtgt ctgaatcacc agtactttcc agcgtagttt caagtatcac
     9121 aatacctgca gttgtctcaa tatctactac atccccgtca cttcaagtcc ccacatccac
     9181 atctgagatc gttgtttcta gtacagcact gtatccttca gtaacagttt cagcaacttc
     9241 agcctctgca gggggcagta ctgctacccc aggtcctaag cctccagctg tagtatctca
     9301 gcaggcagca ggcagcacta ctgtgggagc cacattaaca tcagtttcta ccaccacttc
     9361 attcccaagc acagcttcac agctgtgcat tcagcttagc agcagtactt ctactcctac
     9421 tttagctgaa accgtggtag ttagcgcaca ctcactagat aagacatctc atagcagtac
     9481 aactggattg gctttctccc tctctgcacc atcttcctct tcctctcctg gagcaggagt
     9541 gtctagttat atttctcagc ctggtgggct gcatcctttg gtcattccat cagtgatagc
     9601 ttctactcct attcttcccc aagcagcagg acctacttct acacctttat taccccaagt
     9661 acctagtatc ccacccttgg tacagcctgt tgccaatgtg cctgctgtac agcagacact
     9721 aattcatagt cagcctcaac cagctttgct tcccaaccag ccccatactc attgtcctga
     9781 agtagattct gatacacaac ccaaagctcc tggaattgat gacataaaga ctctagaaga
     9841 aaagctgcgg tctctgttca gtgaacacag ctcatctgga gctcagcatg cctctgtctc
     9901 actggagacc tcactagtca tagagagcac tgtcacacca ggcatcccaa ctactgctgt
     9961 tgcaccaagc aaactcctga cttctaccac aagtacttgc ttaccaccaa ccaatttacc
    10021 actaggaaca gttgctttgc cagttacacc agtggtcaca cctgggcaag tttctacccc
    10081 agtcagcact actacatcag gagtgaaacc tggaactgct ccctccaagc cacctctaac
    10141 taaggctccg gtgctgccag tgggtactga acttccagca ggtactctac ccagcgagca
    10201 gctgccacct tttccaggac cttctctaac ccagtcccag caacctctag aggatcttga
    10261 tgctcaattg agaagaacac ttagtccaga gatgatcaca gtgacttctg cggttggtcc
    10321 tgtgtccatg gcggctccaa cagcaatcac agaagcagga acacagcctc agaagggtgt
    10381 ttctcaagtc aaagaaggcc ctgtcctagc aactagttca ggagctggtg tttttaagat
    10441 gggacgattt caggtttctg ttgcagcaga cggtgcccag aaagagggta aaaataagtc
    10501 agaagatgca aagtctgttc attttgaatc cagcacctca gagtcctcag tgctatcaag
    10561 tagtagtcca gagagtacct tggtgaaacc agagccgaat ggcataacca tccctggtat
    10621 ctcttcagat gtgccagaga gtgcccacaa aactactgcc tcagaggcaa agtcagacac
    10681 tgggcagcct accaaggttg gacgttttca ggtgacaact acagcaaaca aagtgggtcg
    10741 tttctctgta tcaaaaactg aggacaagat cactgacaca aagaaagaag gaccagtggc
    10801 atctcctcct tttatggatt tggaacaagc tgttcttcct gctgtgatac caaagaaaga
    10861 gaagcctgaa ctgtcagagc cttcacatct aaatgggccg tcttctgacc cggaggccgc
    10921 ttttttaagt agggatgtgg atgatggttc cggtagtcca cactcgcccc atcagctgag
    10981 ctcaaagagc cttcctagcc agaatctaag tcaaagcctt agtaattcat ttaactcctc
    11041 ttacatgagt agcgacaatg agtcagatat cgaagatgaa gacttaaagt tagagctgcg
    11101 acgactacga gataaacatc tcaaagagat tcaggacctg cagagtcgcc agaagcatga
    11161 aattgaatct ttgtatacca aactgggcaa ggtgccccct gctgttatta ttcccccagc
    11221 tgctcccctt tcagggagaa gacgacgacc cactaaaagc aaaggcagca aatctagtcg
    11281 aagcagttcc ttggggaata aaagccccca gctttcaggt aacctgtctg gtcagagtgc
    11341 agcttcagtc ttgcaccccc agcagaccct ccaccctcct ggcaacatcc cagagtccgg
    11401 gcagaatcag ctgttacagc cccttaagcc atctccctcc agtgacaacc tctattcagc
    11461 cttcaccagt gatggtgcca tttcagtacc aagcctttct gctccaggtc aaggaaccag
    11521 cagcacaaac actgttgggg caacagtgaa cagccaagcc gcccaagctc agcctcctgc
    11581 catgacgtcc agcaggaagg gcacattcac agatgacttg cacaagttgg tagacaattg
    11641 ggcccgagat gccatgaatc tctcaggcag gagaggaagc aaagggcaca tgaattacga
    11701 gggccctgga atggcaagga agttctctgc acctgggcaa ctgtgcatct ccatgacctc
    11761 gaacctgggt ggctctgccc ccatctctgc agcatcagct acctctctag gtcacttcac
    11821 caagtctatg tgccccccac agcagtatgg ctttccagct accccatttg gcgctcaatg
    11881 gagtgggacg ggtggcccag caccacagcc acttggccag ttccaacctg tgggaactgc
    11941 ctccttgcag aatttcaaca tcagcaattt gcagaaatcc atcagcaacc ccccaggctc
    12001 caacctgcgg accacttagt aatgaactag tccgtaactt gaaagtattt cgatttcttg
    12061 gctttatata tcttgtggaa aggacgaggc tgatgtcagg gatggtgtga cacgcgttaa
    12121 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
    12181 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
    12241 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
    12301 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
    12361 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
    12421 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
    12481 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
    12541 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
    12601 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
    12661 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
    12721 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
    12781 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
    12841 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
    12901 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
    12961 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
    13021 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
    13081 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
    13141 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    13201 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
    13261 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
    13321 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
    13381 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
    13441 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
    13501 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
    13561 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
    13621 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
    13681 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
    13741 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
    13801 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
    13861 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
    13921 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
    13981 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
    14041 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
    14101 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
    14161 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
    14221 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
    14281 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
    14341 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
    14401 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
    14461 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
    14521 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
    14581 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
    14641 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
    14701 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
    14761 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
    14821 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
    14881 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
    14941 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
    15001 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
    15061 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
    15121 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
    15181 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    15241 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    15301 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    15361 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    15421 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    15481 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    15541 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    15601 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    15661 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    15721 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    15781 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    15841 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    15901 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    15961 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    16021 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
    16081 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
    16141 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
    16201 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
    16261 agctgcaagc
//
