LOCUS       sequence-404160-       12175 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12175)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1730
FEATURES             Location/Qualifiers
     source          1..12175
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    8032..8620
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8085..8105)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8503..8514)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8692..8925
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9003..9124
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9040..9059)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9094..9113
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9164..9299
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9319..9338)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9320..9338)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9348..9365)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9348..9364)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9357..9379)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9506..9961
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9593..9612)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9803..9824
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9987..10091
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10092..10160,10161..10952)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10310..10329)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11123..11711
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11612..11631
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11865..11882
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11999..12020
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12035..12040,12041..12058,12059..12065)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12073..12089
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12078..12100
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12097..12113
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12097..12113
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12132..12152
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12134..12152
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgaca gtgaagttgg gcgacggcgg
     4141 cagcggggag gacgggctca agaagctggg caagcgggcg gccgatgagg agtccctgga
     4201 aggagaaggg gccggcggcg cagacgcggc cgaggagagc agcggcacaa agagggacga
     4261 gaagaccccc cgggccggcg ccgacggccc ccccgcgccc cccggcgcgc cgcaggcgcc
     4321 gtccccgccg cagggcagcc cccaggacca gcaccacttc ctccggtcca gcgtgcggcc
     4381 gcagagcaag aggcccagga aggatcctcc gagcgctgtg gggagcggca acgccggtgg
     4441 ctcggggccc cgcggaaaag gagctgaagg tggtggatca tcgtctggaa atgtgtctgg
     4501 ggttgcccct gctgcccctg cagggggctc gcgctcctcc tcccggaact tagggtcttc
     4561 tggtggcgag aaggaagaag gcaaaaaggt ccggcggcag tgggagtcgt ggagcacaga
     4621 ggacaagaac accttcttcg aggggctgta cgagcatggg aaagactttg aagcgattca
     4681 gaacaacatt gcgctgaagt acaagaagaa aggcaagcca gcaagcatgg tgaagaacaa
     4741 ggagcaggtc cgccacttct actaccgcac ctggcacaag atcaccaagt acatcgactt
     4801 tgatcatgtg ttctctcgag gcctgaagaa gtcatcccag gaactgtatg gcctgatctg
     4861 ctatggcgag ctgcgcaaga agattggggg ctgtatggat gacaagaatg caacaaagct
     4921 gaatgaactc attcaggttg gggccaccac tgtacgttac aaagggcgga acctgcggat
     4981 caaagcgccc atgtgccggg ccctgaagaa gctgtgcgat ccagatggct tgagtgatga
     5041 agaggaccag aagccagtgc gcctgcctct gaaagtccct atagagctac agccgcggaa
     5101 caaccacgcc tgggcccgtg tgcagagcct tgcccagaac ccacgcctca ggatgatcgt
     5161 ggagctacat cgaaaggtct ccagcctcat cgaattcttg aagcagaagt gggcgctcca
     5221 tgaggtgcga gttcggaaga cactcgagga gcggcagctg caggactcat gctccgcacc
     5281 gatgcaggag aaggtgacac tgcacttgtt cccaggcgag aactgtacac tgacaccgct
     5341 gccgggcgtg gctcgcgtgg tgcactccaa ggccttctgc acagtgcact ggcaggaggg
     5401 cggccggtgc aagcagagtg ccaaggacgc ccacgtgctg cccccagccc agatcctggg
     5461 catccagagt gggcagggca cggcccgggg ccaggtgaaa tgcccgcgga gcggagctga
     5521 gggcaagggt gtggggcggc cccctcctgc ggctgacgcc ttgcagagct ccggagagag
     5581 ttcccccgaa agcgcccccg gggagggggc tgccctaagc ttgagcagcc cggacgctcc
     5641 tgacaggcct cctcccaggc accaggacac tgggccatgt cttgagaaga cccctgcaga
     5701 aggcagggac agtcccaccc gggagccagg ggccttgccg tgtgcctgtg gccagctccc
     5761 agacctggag gacgagctct cgcttctaga ccccttgccc cgctacctaa agtcctgtca
     5821 ggacctcatt gtccccgagc agtgccgctg tgcggacaca cggcctggga gcgagcagcc
     5881 ccctctgggc ggggcggcct ccccagaggt gctggctcct gtcagcaagg aggctgctga
     5941 ccttgctccc actggcccat ccccgaggcc cggccccggg ctcctgctgg atgtttgcac
     6001 taaagacttg gcagatgcac ctgcggagga gctccaggag aaggggagcc ccgcggggcc
     6061 tccgccgtct cagggacagc ctgccgccag gcccccgaag gaggtccccg ccagccggct
     6121 ggctcagcag ctccgtgagg agggctggaa cctgcagacc tccgaaagcc tcacgctggc
     6181 cgaagtctac ctcatgatgg gcaagcccag caagctgcag ctggagtacg actggctggg
     6241 gcccggccgc caggaccccc gccccggctc cctacccacc gccctccaca agcagcgcct
     6301 cctcagctgc ctcctgaagc tcatttccac cgaggtcaac cccaagctgg ctctggaagc
     6361 aaacaccatc tctacagcct cagtaaggcc cgcccaggag gagcagtcga tgacgccccc
     6421 agggaaggtg gtgaccgtca gctctcgcag cccccgctgc cctcggaacc aggcctccct
     6481 ccgcagcagc aagaccttcc cgcccagctc tgcaccctgc tcctcaggtt tgagaaaccc
     6541 tccaagaccc ctcttggtgc ctggtccctc cagcacagga agcaatgact cagatggagg
     6601 cctttttgct gtcccgacaa ccttgccacc caacagccga cacgggaagc tcttctctcc
     6661 cagtaaagaa gcagagctga ctttccgcca gcatctgaac tccatcagta tgcagtcgga
     6721 tttcttcctg ccaaagcccc ggaagctgcg gaaccggcac ctgcggaagc cactggtggt
     6781 ccagagaaca ctgctcccta gaccatcgga aaaccagtcc cacaacgttt gttccttctc
     6841 catcctgtct aactcttccg taactgggag aggttcgttc cggcccatcc agtcttctct
     6901 gaccaaagca gctctgtctc ggccgatcgt gcccaaggtc cttccacccc aggccacgag
     6961 tcacctggcc agtgctatcg acttagcagc tacaagtgcc ggcatccttt ccgggaaccc
     7021 cctccctgcc ttggacaccg agggcttgtc tggcatctct ccactgtctt cagacgaggt
     7081 gacgggtgcc atctcggggc aggactctac tggaactcac caggatggag acaccctccc
     7141 caccgtgggg ggctccgacc catttgtcag catcccttcg aggcctgagc aggagccagt
     7201 ggcagacagt ttccagggct catctgttct ctccttatct gagctgccca aggcccctct
     7261 ccagaatggc ctctccatac cgctgtcctc gtcagagagc tccagcaccc ggctgtctcc
     7321 accagacgtc tctgctctgc tcgacatctc cctgcccggc ccacctgagg atgcgctgtc
     7381 acagggcgag cctgccacac acattagcga ctccatcatt gagatcgcca tcagctccgg
     7441 tcagtacggt gaaggagtcc ctctttctcc agcaaaactg aatggcagtg acagttccaa
     7501 gagccttccc tccccgtcca gcagccccca gccacactgg atcgcctctc ccacccacga
     7561 cccccagtgg taccccagtg actccaccga ctcctcgctc agcagcctgt ttgcaagctt
     7621 catctcccca gagaagagcc ggaagatgtt gccgactccc attgggacca acagtggcac
     7681 ttccttgctt ggccccagct tgttggatgg aaactcgcgg gactcatttg tgtccaggtc
     7741 cctggctgac gttgcagagg ttgtggattc ccagctggtg tgcatgatga acgaaaacag
     7801 cattgattac atttctcggt tcaatgacct ggcccaagag ctgtccatcg ctgagcctgg
     7861 ccgccgagaa gctctgtttg atggtggtgg aggcggcccc gctgtcagtg acctgtccca
     7921 gtagtaatga actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
     7981 tggaaaggac gaatgcatct agtcaaccac ctgccaacgc gttaagtcga caatcaacct
     8041 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     8101 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     8161 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     8221 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     8281 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     8341 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     8401 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     8461 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     8521 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     8581 cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga
     8641 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     8701 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     8761 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     8821 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     8881 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt
     8941 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     9001 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     9061 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     9121 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     9181 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     9241 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     9301 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     9361 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     9421 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     9481 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     9541 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     9601 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     9661 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     9721 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     9781 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     9841 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     9901 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     9961 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
    10021 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
    10081 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
    10141 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
    10201 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
    10261 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
    10321 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
    10381 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
    10441 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
    10501 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
    10561 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
    10621 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
    10681 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
    10741 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
    10801 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
    10861 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
    10921 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
    10981 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    11041 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    11101 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    11161 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    11221 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
    11281 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    11341 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    11401 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    11461 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    11521 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    11581 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    11641 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    11701 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    11761 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    11821 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    11881 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
    11941 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    12001 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    12061 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
    12121 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagc
//
