LOCUS       sequence-404165-       13909 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13909)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1783
FEATURES             Location/Qualifiers
     source          1..13909
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    9766..10354
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9819..9839)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10237..10248)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10426..10659
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10737..10858
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10774..10793)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10828..10847
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10898..11033
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11053..11072)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11054..11072)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11082..11099)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11082..11098)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11091..11113)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11240..11695
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11327..11346)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11537..11558
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11721..11825
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11826..11894,11895..12686)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12044..12063)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12857..13445
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13346..13365
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13599..13616
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13733..13754
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13769..13774,13775..13792,13793..13799)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13807..13823
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13812..13834
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13831..13847
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13831..13847
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13866..13886
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13868..13886
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgccg cggggccgcc ccccgaagcc
     4141 cagggagagc aaggcgcgcg gcgactccga tggagtttta acattgaatg cggagaacac
     4201 taattatgcc tatcaagttc caaacttcca taaatgtgaa atctgtctac tatcttttcc
     4261 aaaagaatcc cagtttcaac gccacatgag ggatcacgag cgaaatgaca agccacatcg
     4321 atgtgaccag tgcccccaaa catttaatgt tgaattcaac ctgacacttc ataaatgcac
     4381 ccacagcggg gaagatccta cctgccctgt gtgtaacaag aaattctcca gagtggctag
     4441 tctcaaagcg catattatgc tacatgaaaa ggaagagaat ctcatctgtt ctgagtgtgg
     4501 ggatgagttt actctgcaga gtcagctggc cgtgcacatg gaggagcacc gccaggagct
     4561 ggctggaacc cggcagcatg cctgcaaggc ctgcaagaaa gagttcgaga cctcctcgga
     4621 gctgaaggaa cacatgaaga ctcattacaa aattagggta tcaagtacaa ggtcttataa
     4681 ccggaatatc gacagaagtg gattcacgta ttcgtgtccg cactgtggaa agacgtttca
     4741 aaagccaagc cagttaacgc gacacattag gatacacaca ggtgaaaggc cgttcaaatg
     4801 tagtgaatgt ggaaaggctt ttaaccagaa gggggcactg cagacccaca tgatcaagca
     4861 cacaggtgaa aaaccccatg cctgtgcctt ctgtcctgcc gccttctctc agaaagggaa
     4921 tcttcagtcg cacgtgcagc gagtccactc agaggtcaag aatggtccta cctataactg
     4981 tacagaatgt agttgtgtat ttaaaagttt aggcagctta aacacgcata tcagcaagat
     5041 gcatatgggt gggccacaga attcaacaag ttctacagag actgctcatg ttttaacggc
     5101 cacacttttt cagacgttac ctcttcaaca gacggaagcc caagccacgt cggcctcaag
     5161 ccagccgagc tcccaggcgg tgagcgacgt catccagcag ctcctggagc tctcagagcc
     5221 ggcgccggtg gagtcggggc agtccccgca gcctgggcag cagctgagca tcacagtggg
     5281 catcaaccag gacattttac agcaagcctt agaaaacagt gggctgtctt caattccagc
     5341 tgcagcacat cctaatgact cctgccatgc caagacctct gcaccacacg ctcaaaaccc
     5401 agatgtttcc agcgtttcaa atgagcagac ggaccccaca gacgcagagc aagaaaaaga
     5461 acaggaaagc ccggagaaac tggataaaaa agaaaaaaaa atgataaaga agaagtcacc
     5521 gtttctacct ggctccatcc gcgaggagaa cggcgtgcgc tggcatgtgt gtccctactg
     5581 cgccaaggag ttccgcaagc ccagcgacct ggtccgccac atccgcatcc acacccacga
     5641 gaagcccttc aagtgcccgc agtgcttccg cgccttcgcc gtgaagagca cgctgacagc
     5701 gcacatcaag acgcacaccg gcatcaaggc gttcaagtgc cagtactgca tgaagagctt
     5761 ctccacctct ggcagcctca aggtgcacat tcgcctgcac acaggagtta gaccttttgc
     5821 ttgtcctcac tgtgacaaaa aatttcgaac ctcaggccat aggaagactc acattgcttc
     5881 ccactttaaa catacggaat taaggaaaat gaggcaccag cgtaaacctg caaaggtccg
     5941 tgttggcaag acgaatattc cagtccctga tattcctttg caggaaccaa tcctcataac
     6001 tgacttaggt ctcatccagc ccattccaaa aaaccagttt ttccaaagct atttcaataa
     6061 taattttgtc aatgaagcag atagaccata caagtgtttt tactgtcatc gtgcatataa
     6121 aaaatcttgc caccttaaac aacacatcag atcccataca ggtgaaaaac cttttaaatg
     6181 ttctcagtgt ggaagaggct ttgtttctgc aggcgtgctc aaagcacaca tcagaacaca
     6241 cacaggactg aaatctttca agtgtctgat atgtaatggg gctttcacta ctggtggcag
     6301 cttacggcga cacatgggta tccacaacga ccttcgtccc tatatgtgtc cctattgcca
     6361 aaaaacattt aagacttcac taaattgcaa aaagcacatg aaaacccaca gatatgagct
     6421 tgcccagcag ctccaacagc atcagcaggc agcctcgata gatgacagca ctgtagacca
     6481 gcagagcatg caggcctcca ctcaaatgca ggtggagatc gagagcgacg agctgccgca
     6541 gacggcagag gtggtcgcag cgaaccccga ggccatgctg gacctggagc ctcagcatgt
     6601 ggtgggcacg gaggaagcag ggctgggcca gcagttggca gatcagcccc tggaagcaga
     6661 tgaagatggg tttgtggctc cacaggaccc tctgcgaggg cacgtagacc agtttgaaga
     6721 gcagagccct gcgcaacagt ccttcgaacc agcagggcta ccccaaggtt ttacagtgac
     6781 tgatacgtac catcagcagc ctcagtttcc acctgtccaa cagctacagg attccagcac
     6841 acttgagtct caggccctct ccacaagctt ccaccagcag agcttgctgc aggctcccag
     6901 ctctgatggg atgaatgtaa caactcgctt gattcaggag tcatcccaag aggaactgga
     6961 cctgcaggca caaggttccc agtttctgga ggacaacgag gaccagagca ggcgctctta
     7021 caggtgtgac tattgcaaca aaggctttaa gaagtccagc cacctgaagc agcatgtgcg
     7081 gtcgcacacc ggggaaaagc cctacaagtg caagctctgt ggacgcggct ttgtttcctc
     7141 tggggtcctc aagtcccacg agaagacaca cacaggagtg aaggcgttca gctgcagtgt
     7201 gtgcaatgct tccttcacca ccaatggcag cctcacccgg cacatggcca cacatatgag
     7261 catgaagcct tataagtgtc cgttttgtga ggagggtttc cgaactacag tgcattgtaa
     7321 aaagcacatg aagagacacc aaacagtccc ctctgctgtg tcagccactg gagagacaga
     7381 aggaggagac atttgtatgg aggaagagga agaacattct gacagaaatg catcacggaa
     7441 gtctcgtcct gaggtcatca ctttcacgga ggaggagaca gcccagttag ccaagatccg
     7501 gccgcaggag agcgccacgg tgtcagagaa ggtcctggtg cagtccgcgg cagaaaagga
     7561 ccgcatcagt gagctgaggg acaagcaggc ggagctgcag gacgagccca agcacgccaa
     7621 ctgctgcaca tactgcccca agagcttcaa gaaacctagc gacctggtga ggcatgttcg
     7681 aatccatact ggagaaaagc catacaaatg tgatgaatgt ggaaagagtt ttactgtgaa
     7741 atccactctc gattgtcatg tgaagactca cacaggtcag aagctcttca gctgtcacgt
     7801 ctgcagcaac gccttctcca cgaagggaag tctgaaggtc cacatgcgcc tgcacacggg
     7861 agccaagccc ttcaaatgcc cgcattgcga gctgcgtttc cgtacctcgg gtagaagaaa
     7921 gacacacatg cagtttcatt ataaaccaga cccaaagaag gccagaaagc ctatgactcg
     7981 aagctcatcg gaaggactgc agcctgtaaa cctcctcaac tcctcctcta ctgacccaaa
     8041 cgtgtttatc atgaacaact ctgttctaac aggacagttt gatcagaatc tgctgcaacc
     8101 aggactggtg ggccaagcta ttctccctgc ctctgtgtca gctgggggtg acctgaccgt
     8161 gtctctgaca gatgggagcc tggctaccct agaaggcatc cagttacagt tggctgctaa
     8221 cttggttgga ccaaatgtac agatttctgg aatcgatgct gccagcatta ataacattac
     8281 gttgcagatt gatccaagca ttctgcagca gacgctacag cagggcaacc tattggctca
     8341 gcagctcacg ggggagcctg gcctggcccc acagaacagc tctctccaga catcggacag
     8401 cacggtccct gccagtgttg tcatccagcc catctcaggc ctgtccttac agcccacagt
     8461 gacctctgcg aacctgacca taggcccgct gtctgagcag gattcagtgc tgaccactaa
     8521 cagcagtggg acccaagacc tcactcaagt gatgacttcg caaggtctag tgtccccctc
     8581 cggcggtccc cacgagatca ccctgaccat taacaactcc agcctgagcc aggtcctggc
     8641 acaggccgct gggcccactg ccacgtcttc ctcggggtct ccacaggaaa ttaccctgac
     8701 tatctccgaa cttaacacta caagcggaag ccttccttca acaacaccga tgtctccatc
     8761 ggccatctcg actcagaacc tggtcatgtc ctcgtcgggc gtgggaggtg acgctagtgt
     8821 cacgctgacg ctggccgata ctcagggtat gctatctgga ggcctggaca ctgtcacact
     8881 caacatcacc tctcagggtc agcagttccc agcgctcctc acggatccct ctctctcggg
     8941 ccagggtgga gcaggctcgc cgcaagtcat actagtgagc cacacgccac agtcagcgtc
     9001 tgctgcttgt gaagaaatag cctaccaggt agctggcgtc tctgggaacc tggccccggg
     9061 caaccagcca gagaaggagg gccgggcgca ccagtgcctg gagtgtgacc gcgccttctc
     9121 atcggcggcg gtgctcatgc accacagcaa ggaggtgcat ggccgggagc gcatccacgg
     9181 ctgccccgtg tgcaggaagg ccttcaagcg cgccacgcac ctcaaggagc acatgcagac
     9241 acaccaggcc ggcccctctt tgagctccca gaagccaaga gtgtttaaat gtgacacttg
     9301 tgagaaggca tttgccaaac caagccagct ggagcgccac agccgcatac atacagggga
     9361 gcggccgttc cattgcacgc tttgtgagaa agccttcaac cagaagagtg cgctgcaggt
     9421 gcacatgaag aagcacacgg gggagcggcc ctacaagtgt gcctactgcg tcatgggctt
     9481 cacgcagaag agcaacatga agctgcacat gaagcgggcg cacagctatg ctggagctct
     9541 gcaggagtct gcaggtcacc cggagcagga cggggaggag ctgagccgga ccctccacct
     9601 ggaggaggtg gtgcaggagg ccgccggcga gtggcaggcc ctcacccacg tcttctagta
     9661 atgaactagt ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     9721 ggacgagtgc ctacccacta gaagaactac acgcgttaag tcgacaatca acctctggat
     9781 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     9841 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     9901 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     9961 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
    10021 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
    10081 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
    10141 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
    10201 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
    10261 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
    10321 acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca
    10381 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
    10441 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
    10501 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
    10561 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
    10621 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc
    10681 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
    10741 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
    10801 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
    10861 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
    10921 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
    10981 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
    11041 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
    11101 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
    11161 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
    11221 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
    11281 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
    11341 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
    11401 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
    11461 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
    11521 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
    11581 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
    11641 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
    11701 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
    11761 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
    11821 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
    11881 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
    11941 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
    12001 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
    12061 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
    12121 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
    12181 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
    12241 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
    12301 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
    12361 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
    12421 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
    12481 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
    12541 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
    12601 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
    12661 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
    12721 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
    12781 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    12841 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    12901 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    12961 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
    13021 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    13081 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    13141 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    13201 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    13261 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    13321 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    13381 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    13441 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    13501 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    13561 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    13621 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    13681 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
    13741 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
    13801 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
    13861 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagc
//
