LOCUS       sequence-404176-       12775 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12775)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2083
FEATURES             Location/Qualifiers
     source          1..12775
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8632..9220
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8685..8705)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9103..9114)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9292..9525
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9603..9724
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9640..9659)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9694..9713
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9764..9899
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9919..9938)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9920..9938)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9948..9965)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9948..9964)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9957..9979)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10106..10561
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10193..10212)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10403..10424
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10587..10691
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10692..10760,10761..11552)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10910..10929)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11723..12311
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12212..12231
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12465..12482
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12599..12620
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12635..12640,12641..12658,12659..12665)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12673..12689
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12678..12700
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12697..12713
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12697..12713
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12732..12752
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12734..12752
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggat gtagatgctg aaagagagaa
     4141 gataacacag gagatcaagg agctggaaag gattttggat cccggctcct cgggctccca
     4201 cgtggagatc tcagaatcaa gtctcgagtc agattctgaa gcagattcac tgccttctga
     4261 ggacttggat cctgccgatc ccccgatctc ggaagaagaa aggtggggcg aagccagcaa
     4321 tgacgaggac gatcccaagg ataaaaccct ccctgaagac ccagaaacct gcctgcagct
     4381 gaacatggtc taccaggagg tcatccagga gaagctggct gaggccaacc tgctgctggc
     4441 ccagaaccgg gagcagcagg aggaactcat gagggatctg gctgggtcca aaggcaccaa
     4501 ggtgaaagat ggcaaaagcc tgcccccaag cacatacatg gggcacttca tgaagccgta
     4561 tttcaaggac aaggtcacgg gcgtggggcc acctgccaac gaggacacac gagagaaggc
     4621 tgcccagggg atcaaggctt tcgaggagct ccttgtgacc aaatggaaaa actgggaaaa
     4681 ggccttgctc cgaaagtcag tggtgagtga ccgcctgcag cgattgcttc agcccaagtt
     4741 actgaagctc gagtacttgc accagaagca gagcaaagtc tccagtgagc tggagaggca
     4801 agccctggag aagcagggca gggaagccga gaaggagatc caggacatca accagcttcc
     4861 agaagaggcc ttgctgggaa acaggctgga cagccacgac tgggagaaga tttccaatat
     4921 taactttgaa ggcagccgca gtgcagagga gatccggaag ttctggcaga actcggagca
     4981 ccccagcatc aacaagcagg agtggagcag ggaggaggag gagcggctgc aggcgatcgc
     5041 ggctgcacac ggccacctgg agtggcagaa gattgcagag gagctgggga ccagccgcag
     5101 cgccttccag tgcctgcaga aattccagca gcacaacaaa gctctgaaac gcaaggagtg
     5161 gacagaggag gaggaccgca tgctcacgca gctggtgcag gagatgcgcg tcggcagcca
     5221 catcccctac cgcagaattg tctactatat ggaagggaga gactccatgc agctgatcta
     5281 ccgatggacc aagagcttgg atcctggtct gaagaagggt tactgggccc cggaggaaga
     5341 tgctaagttg cttcaagctg ttgccaaata cggggagcag gattggttta aaatccggga
     5401 agaggtgcca ggtaggagcg atgcccagtg ccgagatcgg tatctcagga gattacattt
     5461 cagcttgaaa aagggtcggt ggaatttaaa agaagaggaa cagttaattg aattaataga
     5521 aaaatatggt gtcggtcact gggcaaaaat agcttctgag ctgccccatc ggtctggctc
     5581 ccagtgtctg agcaagtgga agatcatgat ggggaagaag cagggtctcc ggaggcggcg
     5641 gcggagggcc cgtcacagcg tccggtggag ctctaccagc agcagcggca gcagcagtgg
     5701 cagcagtgga gggagcagca gcagcagcag cagcagcagc gaggaggacg agccagagca
     5761 ggcgcaggcc ggggagggtg acagagcgct gctgtcccca cagtacatgg tcccggacat
     5821 ggacctgtgg gttcctgcca ggcagagcac cagccagcca tggagaggag gggcaggggc
     5881 ctggctggga ggccccgctg cctccctcag ccctcccaag gggtccagtg ccagccaggg
     5941 cggcagcaag gaagcttcca ccacagccgc ggctcctgga gaggagacga gtccggtgca
     6001 ggtccctgcc agggcccacg gccctgtccc gaggtctgcc caggcctccc actcagcaga
     6061 cactcgcccg gcgggcgcag agaagcaggc cctggagggt gggaggcgtc tgctgacagt
     6121 gcctgtggag accgtgctga gggtgctcag ggccaacacg gctgctcgga gctgcacaca
     6181 gaaagagcag ctgaggcagc cacccctgcc cacctcatcc ccaggggtca gctctggtga
     6241 cagcgtggcc cgatcccatg tgcagtggct acggcacaga gccacccaga gtgggcagcg
     6301 gcgctggaga cacgctctgc accggaggct cctgaaccgc aggctgctgc tggctgtgac
     6361 cccttgggta ggggacgttg tcgtgccctg cacacaggct tcccagagac ccgccgtagt
     6421 gcagactcaa gcggatggcc tcagggagca gctgcagcag gcccgcctgg ccagcacccc
     6481 tgtgtttacc ctgtttaccc agctgttcca catcgatact gccggctgct tggaggtcgt
     6541 ccgagagagg aaggccctgc cacccaggct gccccaggct ggtgctcggg acccaccagt
     6601 tcatcttctg caggcatcct caagtgccca gagcacccca ggccacctct tcccaaacgt
     6661 gccggctcaa gaagcctcaa agagtgccag ccacaaaggg agccgaagac tggcgtccag
     6721 ccgggtggag cgcaccctac cccaggcgtc cctgctggct tcaaccggcc cccggcccaa
     6781 gcccaagact gtgtcggagc tgcttcagga gaagcggctt caggaggccc gtgccaggga
     6841 ggccacccgg ggcccggtgg tgctcccgtc ccagctgctg gtctcctcgt ctgtgatcct
     6901 ccagccccct ctaccacaca ccccacacgg ccgcccagcc ccgggtccca ccgtcttaaa
     6961 tgtaccgctc tctgggcctg gggcccccgc ggcagccaaa cctggcactt ctggctcctg
     7021 gcaggaggct gggacttcag ccaaggacaa gagactctcc accatgcaag ccctgcccct
     7081 cgctcctgtc ttctcagagg ccgagggcac agcccctgct gcttcccaag cccctgccct
     7141 gggccccggc cagatctctg tgagctgccc cgagagtggt ctcggacagt ctcaggcccc
     7201 cgctgcatcc cggaagcagg gcctgcctga ggcgccaccc tttctccccg cagcccccag
     7261 ccccacccca ctgcccgtcc agcccctcag cctgacgcac ataggagggc cacatgtggc
     7321 gaccagtgtc cccctgcctg tcacctgggt gctcacagcc caggggcttc tccctgttcc
     7381 tgtaccagct gtggtgagcc ttcccaggcc agcagggacc cctggccccg cagggctgct
     7441 ggccactctg ctgcctcccc tgactgagac tcgggcggcc cagggcccca gggccccagc
     7501 gttgagcagc tcttggcagc ccccagccaa tatgaacagg gaaccggagc cttcctgcag
     7561 gacagacacc ccagctcctc ccacacacgc cctctcccaa agtcctgcag aagcggatgg
     7621 cagtgtggcc tttgtccctg gagaggccca ggtggccagg gagatacctg agcccaggac
     7681 gtcctcccac gctgaccctc ctgaagcaga acccccttgg tccgggaggc tgccagcctt
     7741 cggtggtgtc atcccagcaa ctgagccaag ggggacgccg gggtccccct cagggacaca
     7801 ggagcccagg gggcctctgg gcctggagaa gctgcccctg cgccagcctg ggcctgagaa
     7861 gggggccctg gacctggaga agccgcccct accccagcct gggcctgaga agggggccct
     7921 ggacctgggc ctgctgtccc aggagggcga ggcggccaca cagcagtggc tggggggcca
     7981 gcggggggtg cgtgtgcctc ttctgggcag cagactgccc tatcagcccc cagccctgtg
     8041 cagcctgcga gctctgtccg gtctcctact ccacaagaag gccctggagc acaaggccac
     8101 ctccctggtg gtggggggcg aggctgagcg gccggccgga gcactgcaag cctcactggg
     8161 gctggtgcgg gggcagctcc aggacaaccc ggcctacctc ctgttgcggg cgcggttcct
     8221 ggcagccttc accctccctg cgctcctggc caccctggcc ccccaaggcg tccgcaccac
     8281 cctctcagta ccttcgaggg tgggctctga gagtgaggat gaagacctcc tgagtgagct
     8341 ggaacttgca gacagggacg ggcagccggg ctgcacgaca gccacatgcc ccattcaggg
     8401 agccccagac tctggtaaat gctctgcttc ctcctgcctg gatacttcta atgaccctga
     8461 cgacctggac gtgctcagaa cccggcatgc caggcacacc cggaagcgga ggcggctggt
     8521 gtagtaatga actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
     8581 tggaaaggac gacgtgtgtt tacatgcgac tcatttacgc gttaagtcga caatcaacct
     8641 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     8701 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     8761 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     8821 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     8881 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     8941 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     9001 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     9061 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     9121 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     9181 cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga
     9241 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     9301 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     9361 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     9421 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     9481 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt
     9541 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     9601 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     9661 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     9721 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     9781 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     9841 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     9901 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     9961 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
    10021 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
    10081 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
    10141 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
    10201 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
    10261 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
    10321 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
    10381 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
    10441 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
    10501 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
    10561 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
    10621 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
    10681 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
    10741 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
    10801 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
    10861 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
    10921 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
    10981 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
    11041 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
    11101 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
    11161 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
    11221 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
    11281 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
    11341 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
    11401 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
    11461 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
    11521 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
    11581 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    11641 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    11701 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    11761 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    11821 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
    11881 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    11941 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    12001 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    12061 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    12121 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    12181 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    12241 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    12301 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    12361 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    12421 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    12481 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
    12541 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    12601 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    12661 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
    12721 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagc
//
