LOCUS       sequence-404177-       14284 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14284)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 14284)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2038
FEATURES             Location/Qualifiers
     source          1..14284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    10141..10729
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10194..10214)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10612..10623)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10801..11034
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11112..11233
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11149..11168)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11203..11222
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11273..11408
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11428..11447)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11429..11447)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11457..11474)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11457..11473)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11466..11488)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11615..12070
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11702..11721)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11912..11933
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        12096..12200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12201..12269,12270..13061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12419..12438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13232..13820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13721..13740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13974..13991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    14108..14129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14144..14149,14150..14167,14168..14174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14182..14198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14187..14209
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14206..14222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14206..14222
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     14241..14261
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        14243..14261
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggac cctactggaa gtcagttgga
     4141 ttcagatttc tctcagcaag atactccttg cctgataatt gaagattctc agcctgaaag
     4201 ccaggttcta gaggatgatt ctggttctca cttcagtatg ctatctcgac accttcctaa
     4261 tctccagacg cacaaagaaa atcctgtgtt ggatgttgtg tccaatcctg aacaaacagc
     4321 tggagaagaa cgaggagacg gtaatagtgg gttcaatgaa catttgaaag aaaacaaggt
     4381 tgcagaccct gtggattctt ctaacttgga cacatgtggt tccatcagtc aggtcattga
     4441 gcagttacct cagccaaaca ggacaagcag tgttctggga atgtcagtgg aatctgctcc
     4501 tgctgtggag gaagagaagg gagaagagtt ggaacagaag gagaaagaga aggaagaaga
     4561 tacttcaggc aatactacac attcccttgg tgctgaagat actgcctcat cacagttggg
     4621 ttttggggtt ctggaactct cccagagcca ggatgttgag gaaaatactg tgccatatga
     4681 agtggacaaa gagcagctac aatcagtaac caccaactct ggttatacca ggctgtctga
     4741 tgtggatgct aatactgcaa ttaagcatga agaacagtcc aacgaagata tccccatagc
     4801 agaacagtcc agcaaggaca tccctgtgac agcacagccc agtaaggatg tacatgttgt
     4861 aaaagagcaa aatccaccac ctgcaaggtc agaggacatg ccttttagcc ccaaagcatc
     4921 tgttgctgct atggaagcaa aagaacagtt gtctgcacaa gaacttatgg aaagtggact
     4981 gcagattcag aagtcaccag agcctgaggt tttgtcaact caggaagact tgtttgacca
     5041 gagcaataaa acagtatctt ctgatggttg ctctactcct tcaagggagg aaggtgggtg
     5101 ttctttggct tccactcctg ccaccactct gcatctcctg cagctctctg gtcagaggtc
     5161 ccttgttcag gacagtcttt ccacgaattc ttcagatctt gttgctcctt ctcctgatgc
     5221 tttccgatct actcctttta tcgttcctag cagtcccaca gagcaagaag ggagacaaga
     5281 taagccaatg gacacgtcag tgttatctga agaaggagga gagccttttc agaagaaact
     5341 tcaaagtggt gaaccagtgg agttagaaaa cccccctctc ctgcctgagt ccactgtatc
     5401 accacaagcc tcaacaccaa tatctcagag cacaccagtc ttccctcctg ggtcacttcc
     5461 tatcccatcc cagcctcagt tttctcatga catttttatt ccttccccaa gtctggaaga
     5521 acaatcaaat gatgggaaga aagatggaga tatgcatagt tcatctttga cagttgagtg
     5581 ttctaaaact tcagagattg aaccaaagaa ttcccctgag gatcttgggc tatctttgac
     5641 aggggattct tgcaagttga tgctttctac aagtgaatat agtcagtccc caaagatgga
     5701 gagcttgagt tctcacagaa ttgatgaaga tggagaaaac acacagattg aggatacgga
     5761 acccatgtct ccagttctca attctaaatt tgttcctgct gaaaatgata gtatcctgat
     5821 gaatccagca caggatggtg aagtacaact gagtcagaat gatgacaaaa caaagggaga
     5881 tgatacagac accagggatg acattagtat tttagccact ggttgcaagg gcagagaaga
     5941 aacggtagca gaagatgttt gtattgatct cacttgtgat tcggggagtc aggcagttcc
     6001 gtcaccagct actcgatctg aggcactttc tagtgtgtta gatcaggagg aagctatgga
     6061 aattaaagaa caccatccag aggaggggtc ttcagggtct gaggtggaag aaatccctga
     6121 gacaccttgt gaaagtcaag gagaggaact caaagaagaa aatatggaga gtgttccgtt
     6181 gcacctttct ctgactgaaa ctcagtccca agggttgtgt cttcaaaagg aaatgccaaa
     6241 aaaagaatgc tcagaagcta tggaagttga aaccagtgtg attagtattg attcccctca
     6301 aaagttggca atacttgacc aagaattgga acataaggaa caggaagctt gggaagaagc
     6361 tacttcagag gactccagtg ttgtcattgt agatgtgaaa gagccatctc ccagagttga
     6421 tgtttcttgt gaacctttgg agggagtgga gaagtgctca gattcccagt catgggagga
     6481 tattgctcca gaaatagaac catgtgctga gaatagatta gacaccaagg aagaaaagag
     6541 tgtagaatat gaaggagatc tgaaatcagg gactgcagaa acagaacctg tagagcaaga
     6601 ttcttcacag ccttccttac ctttagtgag agcagatgat cctttaagac ttgaccagga
     6661 gttgcagcag ccccaaactc aggagaaaac aagtaattca ttaacagaag actcaaaaat
     6721 ggctaatgca aagcagctaa gctcagatgc agaggcccag aagctgggga agccctctgc
     6781 ccatgcctca caaagcttct gtgaaagttc tagtgaaacc ccatttcatt tcactttgcc
     6841 taaagaaggt gatatcatcc caccattgac tggtgcaacc ccacctctta ttgggcacct
     6901 aaaattggag cccaagagac acagtactcc tattggtatt agcaactatc cagaaagcac
     6961 catagcaacc agtgatgtca tgtctgaaag catggtggag acccatgatc ccatacttgg
     7021 gagtggaaaa ggggattctg gggctgcccc agacgtggat gataaattat gtctaagaat
     7081 gaaactggtt agtcctgaga ctgaggcgag tgaagagtct ttgcagttca acctggaaaa
     7141 gcctgcaact ggtgaaagaa aaaatggatc tactgctgtt gctgagtctg ttgccagtcc
     7201 ccagaagacc atgtctgtgt tgagctgtat ctgtgaagcc aggcaagaga atgaggctcg
     7261 aagtgaggat ccccccacca cacccatcag ggggaacttg ctccactttc caagttctca
     7321 aggagaagag gagaaagaaa aattggaggg tgaccataca atcaggcaga gtcaacagcc
     7381 tatgaagccc attagtcctg tcaaggaccc tgtttctcct gcttcccaga agatggtcat
     7441 acaagggcca tccagtcctc aaggagaggc aatggtgaca gatgtgctag aagaccagaa
     7501 agaaggacgg agtactaata aggaaaatcc tagtaaggcc ttgattgaaa ggcccagcca
     7561 aaataacata ggaatccaaa ccatggagtg ttccttgagg gtcccagaaa ctgtttcagc
     7621 agcaacccag actataaaga atgtgtgtga gcaggggacc agtacagtgg accagaactt
     7681 tggaaagcaa gatgccacag ttcagactga gagggggagt ggtgagaaac cagtcagtgc
     7741 tcctggggat gatacagagt cgctccatag ccagggagaa gaagagtttg atatgcctca
     7801 gcctccacat ggccatgtct tacatcgtca catgagaaca atccgggaag tacgcacact
     7861 tgtcactcgt gtcattacag atgtgtatta tgtggatgga acagaagtag aaagaaaagt
     7921 aactgaggag actgaagagc caattgtaga gtgtcaggag tgtgaaactg aagtttcccc
     7981 ttcacagact gggggctcct caggtgacct gggggatatc agctccttct cctccaaggc
     8041 atccagctta caccgcacat caagtgggac aagtctctca gctatgcaca gcagtggaag
     8101 ctcagggaaa ggagccggac cactcagagg gaaaaccagc gggacagaac ccgcagattt
     8161 tgccttaccc agctcccgag gaggcccagg aaaactgagt cctagaaaag gggtcagtca
     8221 gacagggacg ccagtgtgtg aggaggatgg tgatgcaggc cttggcatca gacagggagg
     8281 gaaggctcca gtcacgcctc gtgggcgtgg gcgaaggggc cgcccacctt ctcggaccac
     8341 tggaaccaga gaaacagctg tgcctggccc cttgggcata gaggacattt cacctaactt
     8401 gtcaccagat gataaatcct tcagccgtgt cgtgccccga gtgccagact ccaccagacg
     8461 aacagatgtg ggtgctggtg ctttgcgtcg tagtgactct ccagaaattc ctttccaggc
     8521 tgctgctggc ccttctgatg gcttagatgc ctcctctcca ggaaatagct ttgtagggct
     8581 ccgtgttgta gccaagtggt catccaatgg ctacttttac tctgggaaaa tcacacgaga
     8641 tgtcggagct gggaagtata aattgctctt tgatgatggg tacgaatgtg atgtgttggg
     8701 caaagacatt ctgttatgtg accccatccc gctggacact gaagtgacgg ccctctcgga
     8761 ggatgagtat ttcagtgcag gagtggtgaa aggacatagg aaggagtctg gggaactgta
     8821 ctacagcatt gaaaaagaag gccaaagaaa gtggtataag cgaatggctg tcatcctgtc
     8881 cttggagcaa ggaaacagac tgagagagca gtatgggctt ggcccctatg aagcagtaac
     8941 acctcttaca aaggcagcag atatcagctt agacaatttg gtggaaggga agcggaaacg
     9001 gcgcagtaac gtcagctccc cagccacccc tactgcctcc agtagcagca gcacaacccc
     9061 tacccgaaag atcacagaaa gtcctcgtgc ctccatggga gttctctcag gcaaaagaaa
     9121 acttatcact tctgaagagg aacggtcccc tgccaagcga ggtcgcaagt ctgccacagt
     9181 aaaacctggt gcagtagggg caggagagtt tgtgagcccc tgtgagagtg gagacaacac
     9241 cggtgaaccc tctgccctgg aagagcagag agggcctttg cctctcaaca agaccttgtt
     9301 tctgggctac gcatttctcc ttaccatggc cacaaccagt gacaagttgg ccagccgctc
     9361 caaactgcca gatggtccta caggaagcag tgaagaagag gaggaatttt tggaaattcc
     9421 tcctttcaac aagcagtata cagaatccca gcttcgagca ggagctggct atatccttga
     9481 agatttcaat gaagcccagt gtaacacagc ttaccagtgt cttctaattg cggatcagca
     9541 ttgtcgaacc cggaagtact tcctgtgcct tgccagtggg attccttgtg tgtctcatgt
     9601 ctgggtccat gatagttgcc atgccaacca gctccagaac taccgtaatt atctgttgcc
     9661 agctgggtac agccttgagg agcaaagaat tctggactgg caaccccgtg aaaatccttt
     9721 ccagaatctg aaggtactct tggtatcaga ccaacagcag aacttcctgg agctctggtc
     9781 tgagatcctc atgactggtg gtgcagcctc tgtgaagcag caccattcaa gtgcccataa
     9841 caaagatatt gctttagggg tatttgatgt ggtggtgacg gacccctcat gcccagcctc
     9901 ggtgctgaag tgtgctgaag cattgcagct gcctgtggtg tcacaagagt gggtgatcca
     9961 gtgcctcatt gttggggaga gaattggatt caagcagcat ccaaaatata aacacgatta
    10021 tgtttctcac tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta
    10081 tatatcttgt ggaaaggacg attacttcgt gcacctacag cgcatacgcg ttaagtcgac
    10141 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
    10201 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
    10261 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
    10321 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
    10381 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
    10441 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
    10501 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
    10561 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
    10621 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
    10681 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
    10741 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
    10801 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
    10861 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
    10921 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
    10981 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt
    11041 atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
    11101 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
    11161 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    11221 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
    11281 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
    11341 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
    11401 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
    11461 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
    11521 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
    11581 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
    11641 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
    11701 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
    11761 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
    11821 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
    11881 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
    11941 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
    12001 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
    12061 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
    12121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
    12181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
    12241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
    12301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
    12361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
    12421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
    12481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
    12541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
    12601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
    12661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
    12721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
    12781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
    12841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
    12901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
    12961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
    13021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
    13081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
    13141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
    13201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
    13261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
    13321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
    13381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
    13441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
    13501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
    13561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
    13621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
    13681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
    13741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
    13801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
    13861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
    13921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
    13981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
    14041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
    14101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
    14161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
    14221 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
    14281 aagc
//
