LOCUS       sequence-404178-       12811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12811)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2280
FEATURES             Location/Qualifiers
     source          1..12811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    8668..9256
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8721..8741)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9139..9150)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9328..9561
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9639..9760
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9676..9695)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9730..9749
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9800..9935
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9955..9974)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9956..9974)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9984..10001)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9984..10000)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9993..10015)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10142..10597
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10229..10248)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10439..10460
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10623..10727
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10728..10796,10797..11588)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10946..10965)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11759..12347
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12248..12267
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12501..12518
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12635..12656
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12671..12676,12677..12694,12695..12701)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12709..12725
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12714..12736
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12733..12749
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12733..12749
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12768..12788
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12770..12788
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgaga gattctgcag aaggtcctaa
     4141 agaagacgaa gagaagcctt cagcctcagc acttgagcag ccggccaccc tccaggaggt
     4201 ggccagtcag gaggtgcctc cagaactagc aacccctgcc cctgcctggg agccacagcc
     4261 agaaccagac gagcgattag aagcggcagc ttgtgaggtg aatgatttgg gggaagagga
     4321 ggaggaggaa gaggaggagg atgaagaaga agaagaagat gatgatgatg atgagttgga
     4381 agacgagggg gaagaagaag ccagcatgcc aaatgaaaat tctgtgaaag agccagaaat
     4441 acggtgtgat gagaagccag aagatttatt agaggaacca aaaacaactt cagaagaaac
     4501 tcttgaagac tgctcagagg taacacctgc catgcaaatc cccagaacta aagaagaggc
     4561 caatggtgat gtatttgaaa cgtttatgtt tccgtgtcaa cattgtgaaa ggaagtttac
     4621 aaccaaacag gggcttgagc gtcacatgca tatccatata tccaccgtca atcatgcttt
     4681 caaatgcaag tactgtggga aagcctttgg cacacagatt aaccggcggc gacatgagcg
     4741 gcgccatgaa gcagggttaa agcggaaacc cagccaaaca ctacagccgt cagaggatct
     4801 ggctgatggc aaagcatctg gagaaaacgt tgcttcaaaa gatgattcga gtcctcccag
     4861 tcttgggcca gactgtctga tcatgaattc agagaaggct tcccaagaca caataaattc
     4921 ttctgtcgta gaagagaatg gggaagttaa agaacttcat ccgtgcaaat attgtaaaaa
     4981 ggtttttgga actcatacta atatgagacg gcatcagcgt agagttcacg aacgtcatct
     5041 gattcccaaa ggtgtacggc gaaaaggagg ccttgaagag ccccagcctc cagcagaaca
     5101 ggcccaggcc acccagaacg tgtatgtacc aagcacagag ccggaggagg aaggggaagc
     5161 agatgatgtg tacatcatgg acatttctag caatatctct gaaaacttaa attactatat
     5221 tgatggtaaa attcaaacta ataacaacac tagtaactgt gatgtgattg agatggagtc
     5281 tgcttcggca gatttgtatg gtataaattg tctgctcact ccagttacag tggaaattac
     5341 tcaaaatata aagaccacac aggtccctgt aacagaagat cttcctaaag agcctttggg
     5401 cagcacaaat agtgaggcca agaagcggag aactgcgagc ccacctgcac tgcccaaaat
     5461 taaggccgaa acagactctg accccatggt cccctcttgc tctttaagtc ttcctcttag
     5521 catatcaaca acagaggcag tgtctttcca caaagagaaa agtgtttatt tgtcatcaaa
     5581 gctcaaacaa cttcttcaaa cccaagataa actaactcct gcagggattt cagcaactga
     5641 aatagctaaa ttaggtcctg tttgtgtgtc tgctcctgca tcaatgttgc ctgtgacctc
     5701 aagtaggttt aagaggcgga ccagctctcc tcccagttct ccacagcaca gtcctgccct
     5761 tcgagacttt ggaaagccaa gtgatgggaa agcagcatgg accgatgccg ggctgacttc
     5821 caaaaaatcc aaattagaaa gtcacagcga ctcaccagca tggagtttgt ctgggagaga
     5881 tgagagagaa actgtgagcc ctccatgctt tgatgaatat aaaatgtcta aagagtggac
     5941 agctagttct gcttttagca gtgtgtgcaa ccagcagcca ctggatttat ccagcggtgt
     6001 caaacagaag gctgagggta caggcaagac tccagtccag tgggaatctg tcttagatct
     6061 cagtgtgcat aaaaagcatt gtagtgactc tgaaggcaag gaattcaaag aaagtcattc
     6121 agtgcagcct acgtgtagtg ctgtaaagaa aaggaaacca accacctgca tgctgcagaa
     6181 ggttcttctc aatgaatata atggcatcga tttacctgta gaaaaccctg cagatgggac
     6241 caggagccca agtccttgta aatccctaga agctcagcca gatcctgacc tcggtccggg
     6301 ctctggtttc cctgccccta ctgttgagtc cacacctgat gtttgtcctt catcacctgc
     6361 cctgcagaca ccctcccttt catccggtca gctgcctcct ctcttgatcc ccacagatcc
     6421 ctcttcccct ccaccctgtc ccccggtatt aactgttgcc actccgcccc ctcccctcct
     6481 tcctaccgta cctcttccag ccccctcttc cagtgcatct ccacacccat gcccctctcc
     6541 actctcaaat gccaccgcac agtccccact tccaattctg tccccaacag tgtccccctc
     6601 tccctctccc attcctcccg tggagcccct gatgtctgcc gcctcacccg ggcctccaac
     6661 actttcttct tcctcctctt catcttcctc ctcctcttcg ttttcttctt catcttcctc
     6721 ctcttctcct tctccacctc ctctctccgc aatatcatct gttgtttcct ctggtgataa
     6781 tctggaggct tctctcccca tgatatcttt caaacaggag gaattagaga atgaaggtct
     6841 gaaacccagg gaagagcccc agtctgctgc tgaacaggat gttgttgttc aggaaacatt
     6901 caacaaaaac tttgtttgca acgtctgtga atcacctttt ctttccatta aagatctaac
     6961 caaacattta tctattcatg ctgaagaatg gcccttcaaa tgtgaatttt gtgtgcagct
     7021 ttttaaggat aaaacggact tgtcagaaca tcgctttttg cttcatggag ttgggaatat
     7081 ctttgtgtgt tctgtttgta aaaaagaatt tgcttttttg tgcaatttgc agcagcacca
     7141 gcgagatctc cacccagata aggtgtgcac acatcacgag tttgaaagcg ggactctgag
     7201 gccccagaac tttacagatc ccagcaaggc ccatgtagag catatgcaga gcttgccaga
     7261 agatccttta gaaacttcta aagaagaaga ggagttaaat gattcctctg aagagcttta
     7321 cacgactata aaaataatgg cttctggaat aaagacaaaa gatccagatg ttcgattggg
     7381 cctcaatcag cattacccaa gctttaaacc acctccattt cagtaccatc accgtaaccc
     7441 catggggatt ggtgtgacag ccacaaattt cactacacac aatattccac agactttcac
     7501 taccgccatt cgctgcacaa agtgtggaaa aggtgtcgac aatatgccgg agttgcacaa
     7561 acatatcctg gcttgtgctt ctgcaagtga caagaagagg tacacgccta agaaaaaccc
     7621 agtaccatta aaacaaactg tgcaacccaa aaatggcgtg gtggttttag ataactctgg
     7681 gaaaaatgcc ttccgacgaa tgggacagcc caaaaggctt aactttagtg ttgagctcag
     7741 caaaatgtcg tcgaataagc tcaaattaaa tgcattgaag aaaaaaaatc agctagtaca
     7801 gaaagcaatt cttcagaaaa acaaatctgc aaagcagaag gccgacttga aaaatgcttg
     7861 tgagtcatcc tctcacatct gcccttactg taatcgagag ttcacttaca ttggaagcct
     7921 gaataaacac gccgccttca gctgtcccaa aaaacccctt tctcctccca aaaaaaaagt
     7981 ttctcattca tctaagaaag gtggacactc atcacctgca agtagtgaca aaaacagtaa
     8041 cagcaaccac cgcagacgga cagcggatgc ggagattaaa atgcaaagca tgcagactcc
     8101 gttgggcaag accagagccc gcagctcagg ccccacccaa gtcccacttc cctcctcatc
     8161 cttcaggtcc aagcagaacg tcaagtttgc agcttcggtg aaatccaaaa aaccaagctc
     8221 ctcctcttta aggaactcca gcccgataag aatggccaaa ataactcatg ttgaggggaa
     8281 aaaacctaaa gctgtggcca agaatcattc tgctcagctt tccagcaaaa catcacggag
     8341 cctgcacgtg agggtacaga aaagcaaagc tgttttacaa agcaaatcca ccttggcgag
     8401 taagaaaaga acagaccggt tcaatataaa atctagagag cggagtgggg ggccagtcac
     8461 ccggagcctt cagctggcag ctgctgctga cttgagtgag aacaagagag aggacggcag
     8521 cgccaagcag gagctgaagg acttcaggaa cttcctgtag taatgaacta gtccgtaact
     8581 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaga agaagaggta
     8641 acaagcccag tgacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     8701 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     8761 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     8821 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     8881 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     8941 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     9001 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     9061 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     9121 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     9181 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     9241 tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta
     9301 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     9361 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     9421 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     9481 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     9541 cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt
     9601 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     9661 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     9721 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     9781 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     9841 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     9901 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     9961 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
    10021 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
    10081 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
    10141 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
    10201 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
    10261 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
    10321 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
    10381 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
    10441 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
    10501 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
    10561 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
    10621 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
    10681 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
    10741 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
    10801 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
    10861 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
    10921 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
    10981 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
    11041 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
    11101 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
    11161 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
    11221 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
    11281 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
    11341 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
    11401 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
    11461 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
    11521 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
    11581 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
    11641 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
    11701 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
    11761 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
    11821 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
    11881 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
    11941 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    12001 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    12061 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    12121 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    12181 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    12241 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    12301 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    12361 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
    12421 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
    12481 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
    12541 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
    12601 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    12661 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    12721 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
    12781 actaaaggga acaaaagctg gagctgcaag c
//
