LOCUS       sequence-404180-       13522 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13522)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2279
FEATURES             Location/Qualifiers
     source          1..13522
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    9379..9967
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9432..9452)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9850..9861)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10039..10272
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10350..10471
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10387..10406)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10441..10460
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10511..10646
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10666..10685)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10667..10685)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10695..10712)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10695..10711)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10704..10726)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10853..11308
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10940..10959)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11150..11171
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11334..11438
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11439..11507,11508..12299)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11657..11676)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12470..13058
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12959..12978
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13212..13229
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13346..13367
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13382..13387,13388..13405,13406..13412)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13420..13436
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13425..13447
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13444..13460
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13444..13460
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13479..13499
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13481..13499
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgaat cagaacacta ctgagcctgt
     4141 ggcggccacc gagaccctgg ctgaggtacc cgaacatgtg ctgcgaggac ttccggagga
     4201 agtgaggctt ttcccttctg ctgttgacaa gacccggatt ggtgtctggg ccactaaacc
     4261 aattttaaaa ggcaaaaaat ttgggccatt tgttggtgat aagaaaaaaa gatctcaggt
     4321 taagaataat gtatacatgt gggaggtgta ttacccaaat ttgggatgga tgtgcattga
     4381 tgccactgat ccagagaagg gaaactggct gcgatatgtg aattgggctt gctcaggaga
     4441 agagcaaaat ttattcccac tggaaatcaa cagagccatt tactataaaa ctttaaagcc
     4501 aatcgcgccg ggcgaggagc tcctggtctg gtacaatggg gaagacaacc ctgagatagc
     4561 agctgcgatt gaggaagagc gagccagcgc ccggagcaag cggagctccc ccaagagccg
     4621 gaaagggaag aaaaaatccc aggaaaataa aaacaaagga aacaaaatcc aagacataca
     4681 actgaagaca agtgagccag atttcacctc tgcaaatatg agagattctg cagaaggtcc
     4741 taaagaagac gaagagaagc cttcagcctc agcacttgag cagccggcca ccctccagga
     4801 ggtggccagt caggaggtgc ctccagaact agcaacccct gcccctgcct gggagccaca
     4861 gccagaacca gacgagcgat tagaagcggc agcttgtgag gtgaatgatt tgggggaaga
     4921 ggaggaggag gaagaggagg aggatgaaga agaagaagaa gatgatgatg atgatgagtt
     4981 ggaagacgag ggggaagaag aagccagcat gccaaatgaa aattctgtga aagagccaga
     5041 aatacggtgt gatgagaagc cagaagattt attagaggaa ccaaaaacaa cttcagaaga
     5101 aactcttgaa gactgctcag aggtaacacc tgccatgcaa atccccagaa ctaaagaaga
     5161 ggccaatggt gatgtatttg aaacgtttat gtttccgtgt caacattgtg aaaggaagtt
     5221 tacaaccaaa caggggcttg agcgtcacat gcatatccat atatccaccg tcaatcatgc
     5281 tttcaaatgc aagtactgtg ggaaagcctt tggcacacag attaaccggc ggcgacatga
     5341 gcggcgccat gaagcagggt taaagcggaa acccagccaa acactacagc cgtcagagga
     5401 tctggctgat ggcaaagcat ctggagaaaa cgttgcttca aaagatgatt cgagtcctcc
     5461 cagtcttggg ccagactgtc tgatcatgaa ttcagagaag gcttcccaag acacaataaa
     5521 ttcttctgtc gtagaagaga atggggaagt taaagaactt catccgtgca aatattgtaa
     5581 aaaggttttt ggaactcata ctaatatgag acggcatcag cgtagagttc acgaacgtca
     5641 tctgattccc aaaggtgtac ggcgaaaagg aggccttgaa gagccccagc ctccagcaga
     5701 acaggcccag gccacccaga acgtgtatgt accaagcaca gagccggagg aggaagggga
     5761 agcagatgat gtgtacatca tggacatttc tagcaatatc tctgaaaact taaattacta
     5821 tattgatggt aaaattcaaa ctaataacaa cactagtaac tgtgatgtga ttgagatgga
     5881 gtctgcttcg gcagatttgt atggtataaa ttgtctgctc actccagtta cagtggaaat
     5941 tactcaaaat ataaagacca cacaggtccc tgtaacagaa gatcttccta aagagccttt
     6001 gggcagcaca aatagtgagg ccaagaagcg gagaactgcg agcccacctg cactgcccaa
     6061 aattaaggcc gaaacagact ctgaccccat ggtcccctct tgctctttaa gtcttcctct
     6121 tagcatatca acaacagagg cagtgtcttt ccacaaagag aaaagtgttt atttgtcatc
     6181 aaagctcaaa caacttcttc aaacccaaga taaactaact cctgcaggga tttcagcaac
     6241 tgaaatagct aaattaggtc ctgtttgtgt gtctgctcct gcatcaatgt tgcctgtgac
     6301 ctcaagtagg tttaagaggc ggaccagctc tcctcccagt tctccacagc acagtcctgc
     6361 ccttcgagac tttggaaagc caagtgatgg gaaagcagca tggaccgatg ccgggctgac
     6421 ttccaaaaaa tccaaattag aaagtcacag cgactcacca gcatggagtt tgtctgggag
     6481 agatgagaga gaaactgtga gccctccatg ctttgatgaa tataaaatgt ctaaagagtg
     6541 gacagctagt tctgctttta gcagtgtgtg caaccagcag ccactggatt tatccagcgg
     6601 tgtcaaacag aaggctgagg gtacaggcaa gactccagtc cagtgggaat ctgtcttaga
     6661 tctcagtgtg cataaaaagc attgtagtga ctctgaaggc aaggaattca aagaaagtca
     6721 ttcagtgcag cctacgtgta gtgctgtaaa gaaaaggaaa ccaaccacct gcatgctgca
     6781 gaaggttctt ctcaatgaat ataatggcat cgatttacct gtagaaaacc ctgcagatgg
     6841 gaccaggagc ccaagtcctt gtaaatccct agaagctcag ccagatcctg acctcggtcc
     6901 gggctctggt ttccctgccc ctactgttga gtccacacct gatgtttgtc cttcatcacc
     6961 tgccctgcag acaccctccc tttcatccgg tcagctgcct cctctcttga tccccacaga
     7021 tccctcttcc cctccaccct gtcccccggt attaactgtt gccactccgc cccctcccct
     7081 ccttcctacc gtacctcttc cagccccctc ttccagtgca tctccacacc catgcccctc
     7141 tccactctca aatgccaccg cacagtcccc acttccaatt ctgtccccaa cagtgtcccc
     7201 ctctccctct cccattcctc ccgtggagcc cctgatgtct gccgcctcac ccgggcctcc
     7261 aacactttct tcttcctcct cttcatcttc ctcctcctct tcgttttctt cttcatcttc
     7321 ctcctcttct ccttctccac ctcctctctc cgcaatatca tctgttgttt cctctggtga
     7381 taatctggag gcttctctcc ccatgatatc tttcaaacag gaggaattag agaatgaagg
     7441 tctgaaaccc agggaagagc cccagtctgc tgctgaacag gatgttgttg ttcaggaaac
     7501 attcaacaaa aactttgttt gcaacgtctg tgaatcacct tttctttcca ttaaagatct
     7561 aaccaaacat ttatctattc atgctgaaga atggcccttc aaatgtgaat tttgtgtgca
     7621 gctttttaag gataaaacgg acttgtcaga acatcgcttt ttgcttcatg gagttgggaa
     7681 tatctttgtg tgttctgttt gtaaaaaaga atttgctttt ttgtgcaatt tgcagcagca
     7741 ccagcgagat ctccacccag ataaggtgtg cacacatcac gagtttgaaa gcgggactct
     7801 gaggccccag aactttacag atcccagcaa ggcccatgta gagcatatgc agagcttgcc
     7861 agaagatcct ttagaaactt ctaaagaaga agaggagtta aatgattcct ctgaagagct
     7921 ttacacgact ataaaaataa tggcttctgg aataaagaca aaagatccag atgttcgatt
     7981 gggcctcaat cagcattacc caagctttaa accacctcca tttcagtacc atcaccgtaa
     8041 ccccatgggg attggtgtga cagccacaaa tttcactaca cacaatattc cacagacttt
     8101 cactaccgcc attcgctgca caaagtgtgg aaaaggtgtc gacaatatgc cggagttgca
     8161 caaacatatc ctggcttgtg cttctgcaag tgacaagaag aggtacacgc ctaagaaaaa
     8221 cccagtacca ttaaaacaaa ctgtgcaacc caaaaatggc gtggtggttt tagataactc
     8281 tgggaaaaat gccttccgac gaatgggaca gcccaaaagg cttaacttta gtgttgagct
     8341 cagcaaaatg tcgtcgaata agctcaaatt aaatgcattg aagaaaaaaa atcagctagt
     8401 acagaaagca attcttcaga aaaacaaatc tgcaaagcag aaggccgact tgaaaaatgc
     8461 ttgtgagtca tcctctcaca tctgccctta ctgtaatcga gagttcactt acattggaag
     8521 cctgaataaa cacgccgcct tcagctgtcc caaaaaaccc ctttctcctc ccaaaaaaaa
     8581 agtttctcat tcatctaaga aaggtggaca ctcatcacct gcaagtagtg acaaaaacag
     8641 taacagcaac caccgcagac ggacagcgga tgcggagatt aaaatgcaaa gcatgcagac
     8701 tccgttgggc aagaccagag cccgcagctc aggccccacc caagtcccac ttccctcctc
     8761 atccttcagg tccaagcaga acgtcaagtt tgcagcttcg gtgaaatcca aaaaaccaag
     8821 ctcctcctct ttaaggaact ccagcccgat aagaatggcc aaaataactc atgttgaggg
     8881 gaaaaaacct aaagctgtgg ccaagaatca ttctgctcag ctttccagca aaacatcacg
     8941 gagcctgcac gtgagggtac agaaaagcaa agctgtttta caaagcaaat ccaccttggc
     9001 gagtaagaaa agaacagacc ggttcaatat aaaatctaga gagcggagtg gggggccagt
     9061 cacccggagc cttcagctgg cagctgctgc tgacttgagt gagaacaaga gagaggacgg
     9121 cagcgccaag caggagctga aggacttcag ctacagcctc cgcttggcgt cccgatgctc
     9181 tccaccagcg gccccgtaca tcaccaggca gtataggaag gtcaaagctc cagctgcagc
     9241 ccagttccag ggaccattct tcaaagagta gtaatgaact agtccgtaac ttgaaagtat
     9301 ttcgatttct tggctttata tatcttgtgg aaaggacgag acgtgtgatc ccagcatgta
     9361 cctacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     9421 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     9481 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     9541 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     9601 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     9661 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     9721 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     9781 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     9841 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     9901 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     9961 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
    10021 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
    10081 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
    10141 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
    10201 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
    10261 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
    10321 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
    10381 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
    10441 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
    10501 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
    10561 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
    10621 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
    10681 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
    10741 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
    10801 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
    10861 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
    10921 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
    10981 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
    11041 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
    11101 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
    11161 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
    11221 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
    11281 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
    11341 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
    11401 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
    11461 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
    11521 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
    11581 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
    11641 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
    11701 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
    11761 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
    11821 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
    11881 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
    11941 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
    12001 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
    12061 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
    12121 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
    12181 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
    12241 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
    12301 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
    12361 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
    12421 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
    12481 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
    12541 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    12601 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    12661 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    12721 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    12781 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    12841 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    12901 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    12961 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    13021 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    13081 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    13141 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
    13201 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
    13261 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
    13321 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
    13381 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
    13441 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
    13501 aacaaaagct ggagctgcaa gc
//
