LOCUS       sequence-404182-       16735 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16735)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 16735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2475
FEATURES             Location/Qualifiers
     source          1..16735
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    12592..13180
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(12645..12665)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(13063..13074)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             13252..13485
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    13563..13684
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13600..13619)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13654..13673
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      13724..13859
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(13879..13898)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(13880..13898)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(13908..13925)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13908..13924)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13917..13939)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      14066..14521
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14153..14172)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14363..14384
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        14547..14651
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(14652..14720,14721..15512)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(14870..14889)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      15683..16271
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     16172..16191
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     16425..16442
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    16559..16580
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(16595..16600,16601..16618,16619..16625)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16633..16649
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     16638..16660
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     16657..16673
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     16657..16673
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     16692..16712
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        16694..16712
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgcgg ctcgggggcg ggcagctggt
     4141 gtcagaggag ctgatgaacc tgggcgagag cttcatccag accaacgacc cgtcgctgaa
     4201 gctcttccag tgcgccgtct gcaacaagtt cacgacggac aacctggaca tgctgggcct
     4261 gcacatgaac gtggagcgca gcctgtcgga ggacgagtgg aaggcggtga tgggggactc
     4321 ataccagtgc aagctctgcc gctacaacac ccagctcaag gccaacttcc agctgcactg
     4381 caagacagac aagcacgtgc agaagtacca gctggtggcc cacatcaagg agggcggcaa
     4441 ggccaacgag tggaggctca agtgtgtggc catcggcaac cccgtgcacc tcaagtgcaa
     4501 cgcctgtgac tactacacca acagcctgga gaagctgcgg ctgcacacgg tcaactccag
     4561 gcacgaggcc agcctgaagt tgtacaagca cctgcagcag catgagagtg gtgtagaagg
     4621 tgagagctgc tactaccact gcgttctgtg caactactcc accaaggcca agctcaacct
     4681 catccagcat gtgcgctcca tgaagcacca gcgaagcgag agcctgcgaa agctgcagcg
     4741 gctgcagaag ggccttccag aggaggacga ggacctgggg cagatcttca ccatccgcag
     4801 gtgcccctcc acggacccag aagaagccat tgaagatgtt gaaggaccca gtgaaacagc
     4861 tgctgatcca gaggagcttg ctaaggacca agagggcgga gcatcgtcca gccaagcaga
     4921 gaaggagctg acagattctc ctgcaacctc caaacgcatc tccttcccag gtagctcaga
     4981 gtctcccctc tcttcgaagc gaccaaaaac agctgaggag atcaaaccgg agcagatgta
     5041 ccagtgtccc tactgcaagt acagtaatgc cgatgtcaac cggctccggg tgcatgccat
     5101 gacgcagcac tcggtgcaac ccatgcttcg ctgccccctg tgccaggaca tgctcaacaa
     5161 caagatccac ctccagctgc acctcaccca cctccacagc gtggcacctg actgcgtgga
     5221 gaagctcatt atgacggtga ccacccctga gatggtgatg ccaagcagca tgttcctccc
     5281 agcagctgtt ccagatcgag atgggaattc caatttggaa gaggcaggaa agcagcctga
     5341 aacctcagag gatctgggaa agaacatctt gccatccgca agcacagagc aaagcggaga
     5401 tttgaaacca tcccctgctg acccaggctc tgtgagagaa gactcaggct tcatctgctg
     5461 gaagaagggg tgcaaccagg ttttcaaaac ttctgctgcc cttcagacgc attttaatga
     5521 agtgcatgcc aagaggcctc agctgccggt gtcagatcgc catgtgtaca agtaccgctg
     5581 taatcagtgt agcctggcct tcaagaccat tgaaaagttg cagctccatt ctcagtacca
     5641 tgtgatcaga gctgccacca tgtgctgtct ttgtcagcgc agtttccgaa ctttccaggc
     5701 tctgaagaag caccttgaga caagccacct ggagctgagt gaggctgaca tccaacagct
     5761 ttatggtggc ctgctggcca atggggacct cctggcaatg ggagacccca ctctggcaga
     5821 ggaccatacc ataattgttg aggaagacaa ggaggaagag agtgacttgg aagataaaca
     5881 gagcccaacg ggcagtgact ctgggtcagt acaagaagac tcgggctcag agccaaagag
     5941 agctctgcct ttcagaaaag gtcccaattt tactatggaa aagttcctag acccttctcg
     6001 cccttacaag tgtaccgtct gcaaggaatc tttcactcaa aagaatatcc tgctagtaca
     6061 ctacaattct gtctcccacc tgcataagtt aaagagagcc cttcaagaat cagcaaccgg
     6121 tcagccagaa cccaccagca gcccagacaa caaacctttt aagtgtaaca cttgtaatgt
     6181 ggcctacagc cagagttcca ctctggagat ccatatgagg tctgtgttac atcaaaccaa
     6241 ggcccgggca gccaagctgg aggctgcaag tggcagcagc aatgggactg ggaacagcag
     6301 cagtatttcc ttgagctcct ccacgccaag tcctgtgagc accagtggca gtaacacctt
     6361 taccacctcc aatccaagca gtgctggcat tgctccaagc tctaacttac taagccaagt
     6421 gcccactgag agtgtaggga tgccacccct ggggaatcct attggtgcca acattgcttc
     6481 cccttcagag cccaaagagg ccaatcggaa gaaactggca gatatgattg catccaggca
     6541 gcagcaacaa cagcagcagc aacagcaaca acaacaacaa caacaacaac aacaagcaca
     6601 aacgctggcc caggcccagg ctcaagttca agctcacctg cagcaggagc tgcagcaaca
     6661 ggctgccctg atccagtctc agctgtttaa ccccaccctc cttcctcact tccccatgac
     6721 aactgagacc ctgctgcaac tacagcagca gcagcacctc ctcttccctt tctacatccc
     6781 cagtgctgag ttccagctta accccgaggt gagcttgcca gtgaccagtg gggcactgac
     6841 actgactggg acaggcccag gcctgctgga agatctgaag gctcaggttc aggtcccaca
     6901 gcagagccat cagcagatct tgccgcagca gcagcagaac caactctcta tagcccagag
     6961 tcactctgcc ctccttcagc caagccagca ccccgaaaag aagaacaaat tggtcatcaa
     7021 agaaaaggaa aaagaaagcc agagagagag ggacagcgcc gaggggggag agggcaacac
     7081 cggtccgaag gaaacactgc cagatgcctt gaaggccaaa gagaagaaag agttggcacc
     7141 agggggtggt tctgagcctt ccatgctccc tccacgcatt gcttcagatg ccagagggaa
     7201 cgccaccaag gccctgctgg agaactttgg ctttgagttg gtcatccagt ataatgagaa
     7261 caagcagaag gtgcagaaaa agaatgggaa gactgaccag ggagagaacc tggaaaagct
     7321 cgagtgtgac tcctgcggca agttgttttc caacatcttg attttaaaga gtcatcaaga
     7381 gcacgttcat cagaattact ttcctttcaa acagctcgag aggtttgcca aacagtacag
     7441 agaccactac gataaactgt acccactgag gccccagacc ccagagccac caccacctcc
     7501 ccctccaccc cctccacccc cacttccggc agcgccgcct cagccggcgt ccacaccagc
     7561 catccccgca tcagccccac ccatcacctc acctacaatt gcaccggccc agccatcagt
     7621 gccgctcacc cagctctcca tgccgatgga gctgcccatc ttctcgccgc tgatgatgca
     7681 gacgatgccg ctgcagacct tgccggctca gctacccccg cagctgggac ctgtggagcc
     7741 tctgcctgcg gacctggccc aactctacca gcatcagctc aatccaaccc tgctccagca
     7801 gcagaacaag aggcctcgca ccaggatcac agatgatcag ctccgagtct tgcggcaata
     7861 ttttgacatt aacaactccc ccagtgaaga gcaaataaaa gagatggcag acaagtccgg
     7921 gttgccccag aaagtgatca agcactggtt caggaacact ctcttcaaag agaggcagcg
     7981 taacaaggac tccccttaca acttcagtaa tcctcctatc accagcctgg aggagctcaa
     8041 gattgactcc cggccccctt cgccggaacc tccaaagcag gagtactggg gaagcaagag
     8101 gtcttcaaga acaaggttta cggactacca gctgagggtc ttacaggact tcttcgatgc
     8161 caatgcttac ccaaaggatg atgaatttga gcaactctct aatttactga accttccaac
     8221 ccgagtgata gtggtgtggt ttcagaatgc ccgacagaag gccaggaaga attatgagaa
     8281 tcagggagag ggcaaagatg gagagcggcg tgagcttaca aatgatagat acattcgaac
     8341 aagcaacttg aactaccagt gcaaaaaatg tagcctggtg tttcagcgca tctttgatct
     8401 catcaagcac cagaagaagc tgtgttacaa ggatgaggat gaggaggggc aggacgacag
     8461 ccaaaatgag gattccatgg atgccatgga aatcctgacg cctaccagct catcctgcag
     8521 taccccgatg ccctcacagg cttacagcgc cccagcacca tcagccaata atacagcttc
     8581 ctccgctttc ttgcagctta cagcggaggc tgaggaactg gccaccttca attcaaaaac
     8641 agaggcaggc gatgagaaac caaagctggc ggaagctccc agtgcacagc caaaccaaac
     8701 ccaagaaaag caaggacaac caaagccaga gctgcagcag caagagcagc ccgagcagaa
     8761 gaccaacact ccccagcaga agctccccca gctggtgtcc ctgccttcgt tgccacagcc
     8821 tcctccacaa gcgccccctc cacagtgccc cttaccccag tcgagcccca gtccttccca
     8881 gctctcccac ctgcccctca agcccctcca cacatcaact cctcaacagc tcgcaaacct
     8941 acctcctcag ctaatcccct accagtgtga ccagtgtaag ttggcatttc cgtcatttga
     9001 gcactggcag gagcatcagc agctccactt cctgagcgcg cagaaccagt tcatccaccc
     9061 ccagtttttg gacaggtccc tggatatgcc tttcatgctc tttgatccca gtaacccact
     9121 cctggccagc cagctgctct ctggggccat acctcagatt ccagcaagct cagccacttc
     9181 tccttcaact ccaacctcca caatgaacac tctcaagagg aagctggagg aaaaggccag
     9241 tgcaagccct ggcgaaaacg acagtgggac aggaggagaa gagcctcaga gagacaagcg
     9301 tttgagaaca accatcacac cggaacaact agaaattctc taccagaagt atctactgga
     9361 ttccaatccg actcgaaaga tgttggatca cattgcacac gaggtgggct tgaagaaacg
     9421 tgtggtacaa gtctggtttc agaacacccg agctcgggaa aggaaaggac agttccgggc
     9481 tgtaggccca gcgcaggccc acaggagatg ccctttttgc agagcgctct tcaaagccaa
     9541 gactgctctt gaggctcata tccggtcccg tcactggcat gaagccaaga gagctggcta
     9601 caacctaact ctgtctgcga tgctcttaga ctgtgatggg ggactccaga tgaaaggaga
     9661 tatttttgac ggaactagct tttcccacct acccccaagc agtagtgatg gtcagggtgt
     9721 ccccctctca cctgtgagta aaaccatgga attgtcaccc agaactcttc taagcccttc
     9781 ctccattaag gtggaaggga ttgaagactt tgaaagcccc tccatgtcct cagttaatct
     9841 aaactttgac caaactaagc tggacaacga tgactgttcc tctgtcaaca cagcaatcac
     9901 agataccaca actggagacg agggcaacgc agataacgac agtgcaacgg gaatagcaac
     9961 tgaaaccaaa tcctcttctg cacccaacga agggttgacc aaagcggcca tgatggcaat
    10021 gtctgagtat gaagatcggt tgtcatctgg tctggtcagc ccggccccga gcttttatag
    10081 caaggaatat gacaatgaag gtacagtgga ctacagtgaa acctcaagcc ttgcagatcc
    10141 ctgctccccg agtcctggtg cgagtggatc tgcaggcaaa tctggtgaca gcggagatcg
    10201 gcctgggcag aaacgttttc gcactcaaat gaccaatctg cagctgaagg tcctcaagtc
    10261 atgctttaat gactacagga cacccactat gctagaatgt gaggtcctgg gcaatgacat
    10321 tggactgcca aagagagtcg ttcaggtctg gttccagaat gcccgggcaa aagaaaagaa
    10381 gtccaagtta agcatggcca agcattttgg tataaaccaa acgagttatg agggacccaa
    10441 aacagagtgc actttgtgtg gcatcaagta cagcgctcgg ctgtctgtac gtgaccatat
    10501 cttttcccaa cagcatatct ccaaagttaa agacaccatt ggaagccagc tggacaagga
    10561 gaaagaatac tttgacccag ccaccgtacg tcagttgatg gctcaacaag agttggaccg
    10621 gattaaaaag gccaacgagg tccttggact ggcagctcag cagcaaggga tgtttgacaa
    10681 cacccctctt caggccctta accttcctac agcatatcca gcgctccagg gcattcctcc
    10741 tgtgttgctc ccgggcctca acagcccctc cttgccaggc tttactccat ccaacacagc
    10801 tttaacgtct cctaagccga acttgatggg tctgcccagc acaactgttc cttcccctgg
    10861 cctccccact tctggattac caaataaacc gtcctcagcg tcgctgagct ccccaacccc
    10921 agcacaagcc acgatggcga tgggccctca gcaacccccc cagcagcagc agcagcagca
    10981 gcaaccacag gtgcagcagc ctcccccgcc gccagcagcc cagccgccac ccacaccaca
    11041 gctcccactg caacagcagc agcaacgcaa ggacaaagac agtgagaaag taaaggagaa
    11101 ggaaaaggca cacaaaggga aaggggaacc cctgcctgtc cccaagaagg agaaaggaga
    11161 ggcccccacg gcaactgcag ccacgatctc agccccgctg cccaccatgg agtatgcggt
    11221 agaccctgca cagctgcagg ccctgcaggc cgcgttgact tcggacccca cagcattgct
    11281 cacaagccag ttccttcctt actttgtacc aggcttttct ccttattatg ctccccagat
    11341 ccctggcgcc ctgcagagcg ggtacctgca gcctatgtat ggcatggaag gcctgttccc
    11401 ctacagccct gcactgtcgc aggccctgat ggggctgtcc ccaggctccc tactgcagca
    11461 gtaccagcaa taccagcaga gtctgcagga ggcaattcag cagcagcagc agcggcaact
    11521 acagcagcag cagcagcaaa aagtgcagca gcagcagccc aaagcaagcc aaaccccagt
    11581 cccccccggg gctccttccc cagacaaaga ccctgccaaa gaatccccca aaccagaaga
    11641 acagaaaaac accccccgtg aggtgtcccc cctcctgccg aaactccctg aagagccaga
    11701 agcagaaagc aaaagtgcgg actccctcta cgaccccttc attgttccaa aggtgcagta
    11761 caagttggtc tgccgcaagt gccaggcggg cttcagcgac gaggaggcag cgaggagcca
    11821 cctgaagtcc ctctgcttct tcggccagtc tgtggtgaac ctgcaagaga tggtgcttca
    11881 cgtccccacc ggcggcggcg gcggtggcag tggcggcggc ggcggcggtg gcggcggcgg
    11941 cggcggcggc ggctcgtacc actgcctggc gtgcgagagc gcgctctgtg gggaggaagc
    12001 tctgagtcaa catctcgagt cggccttgca caaacacaga acaatcacga gagcagcaag
    12061 aaacgccaaa gagcacccta gtttattacc tcactctgcc tgcttccccg atcctagcac
    12121 cgcatctacc tcgcagtctg ccgctcactc aaacgacagc ccccctcccc cgtcggccgc
    12181 cgccccctcc tccgcttccc cccacgcctc caggaagtct tggccgcaag tggtctcccg
    12241 ggcttcggca gcgaagcccc cttcttttcc tcctctctcc tcatcttcaa cggttacctc
    12301 aagttcatgc agcacctcag gggttcagcc ctcgatgcca acagacgact attcggagga
    12361 gtctgacacg gatctcagcc aaaagtccga cggaccggcg agcccggtgg agggtcccaa
    12421 agaccccagc tgccccaagg acagtggtct gaccagtgta ggaacggaca ccttcagatt
    12481 gtagtaatga actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
    12541 tggaaaggac gacgagcatt ggactctgat aatgaaacgc gttaagtcga caatcaacct
    12601 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
    12661 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
    12721 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
    12781 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
    12841 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
    12901 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
    12961 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
    13021 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
    13081 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
    13141 cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga
    13201 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
    13261 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
    13321 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
    13381 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
    13441 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt
    13501 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
    13561 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
    13621 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    13681 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
    13741 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
    13801 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
    13861 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
    13921 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
    13981 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
    14041 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
    14101 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
    14161 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
    14221 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
    14281 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
    14341 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
    14401 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
    14461 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
    14521 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
    14581 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
    14641 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
    14701 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
    14761 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
    14821 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
    14881 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
    14941 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
    15001 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
    15061 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
    15121 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
    15181 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
    15241 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
    15301 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
    15361 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
    15421 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
    15481 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
    15541 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    15601 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    15661 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    15721 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    15781 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
    15841 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    15901 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    15961 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    16021 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    16081 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    16141 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    16201 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    16261 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    16321 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    16381 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    16441 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
    16501 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    16561 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    16621 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
    16681 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagc
//
