LOCUS       sequence-404185-       17563 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17563)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 17563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1770
FEATURES             Location/Qualifiers
     source          1..17563
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             9200..9208
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    13420..14008
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(13473..13493)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(13891..13902)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             14080..14313
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    14391..14512
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14428..14447)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14482..14501
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      14552..14687
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(14707..14726)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(14708..14726)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(14736..14753)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(14736..14752)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14745..14767)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      14894..15349
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14981..15000)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15191..15212
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        15375..15479
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(15480..15548,15549..16340)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(15698..15717)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      16511..17099
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     17000..17019
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     17253..17270
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    17387..17408
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(17423..17428,17429..17446,17447..17453)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    17461..17477
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     17466..17488
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     17485..17501
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     17485..17501
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     17520..17540
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        17522..17540
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggag gagaaacagc agattatatt
     4141 ggctaatcaa gatggtggaa cagtggcagg agcagcacct accttctttg tcatcttaaa
     4201 gcagccagga aatggcaaaa ctgatcaagg aattttggtt actaatcagg atgcctgtgc
     4261 tttggctagt agtgtgtcat caccagtaaa atctaaaggg aagatttgcc ttccagctga
     4321 ttgtactgtg ggtggaatca ctgttaccct cgataacaat agtatgtgga atgagttcta
     4381 tcatcgaagc acagagatga ttctgaccaa gcaaggaaga cgcatgtttc cttactgtcg
     4441 ttattggata acaggtttag attcaaattt gaagtatatt cttgtcatgg atatatctcc
     4501 tgtggataac catcgttata agtggaatgg tcgttggtgg gaacctagtg ggaaggctga
     4561 acctcatgtt ttggggaggg ttttcattca tccagaatct ccttccacag gtcattattg
     4621 gatgcatcaa ccagtatctt tctataaact caaacttacc aacaatacac tggaccaaga
     4681 agggcatatc atcttgcact ctatgcatcg ttacctgccg aggcttcatt tggtgcctgc
     4741 agaaaaggct gtggaggtga tacaattaaa tggccctggt gtccacactt ttaccttccc
     4801 acagactgaa ttctttgcag taacagctta tcagaacatt cagattactc agctgaaaat
     4861 agattacaat ccatttgcca aaggctttcg ggatgatggg ctgaataata agccccagag
     4921 agatggaaaa caaaagaaca gctctgacca agaagggaat aatatttcca gttcttctgg
     4981 tcatcgggtc cgtcttacag aaggtcaggg gtcagagata caaccaggtg atttggatcc
     5041 tttgtcaagg ggtcatgaaa catcaggcaa gggtttggag aagacttccc ttaatataaa
     5101 acgagacttt cttggtttca tggatactga ttcagcactt agtgaagttc ctcaattgaa
     5161 gcaagagatt tctgaatgtc ttattgccag cagttttgaa gatgactccc gtgtagcctc
     5221 accgttagac cagaacggaa gcttcaatgt tgttattaaa gaggaacctc tagatgatta
     5281 tgactacgaa cttggtgagt gcccagaagg ggtcactgtg aaacaggaag agacagatga
     5341 agagacggat gtatactcaa acagtgatga tgatcctata ctagagaaac agctaaagag
     5401 gcacaataaa gttgacaacc cagaagctga ccatctatct tctaaatggc ttccaagcag
     5461 cccatcaggt gttgctaaag ctaaaatgtt caaattagac actggaaaga tgccagtagt
     5521 ctatctggag ccctgtgctg tcaccagaag cacagttaag atttctgaac tccccgataa
     5581 catgctttcc acatctcgaa aggataaatc ttctatgttg gcagaattgg aatatttgcc
     5641 tacatacatt gaaaattcca atgagactgc cttctgctta ggcaaggaat cagaaaatgg
     5701 tcttagaaaa cattcaccag atctcagagt ggtacaaaaa tatcccttac tgaaagagcc
     5761 tcagtggaaa tatcctgata tatctgacag cattagcaca gaaagaatac tcgacgattc
     5821 aaaggattca gttggagact cactttcagg aaaagaggac ttgggcagaa agagaacaac
     5881 tatgcttaag attgcaacag ccgcaaaggt agtgaatgct aatcagaatg cctctccaaa
     5941 tgtccctgga aaaagaggaa ggccacgaaa attgaaactc tgtaaggcag gacgaccacc
     6001 taagaacaca ggaaagtctt taatttctac aaagaataca cctgtaagcc ctgggagtac
     6061 ctttccagat gtgaagcctg atctggaaga tgtggatggt gttctctttg tttcctttga
     6121 atcaaaggaa gctctagaca ttcatgcagt tgatgggaca acagaagaat cttctagtct
     6181 ccaggcatca accacaaatg actcaggtta cagagcaaga atttcccagt tggaaaagga
     6241 attgatagaa gatttgaaga ctttgcggca caagcaggtg atacatcctg gtcttcaaga
     6301 agtgggctta aaattgaatt cagtggatcc aacaatgagc attgatctta aatacttggg
     6361 agtacagtta cctttggctc cagctactag ctttcctttt tggaacctta caggaaccaa
     6421 ccctgcctct cctgatgcgg gatttccctt tgtttctagg acagggaaaa ccaatgattt
     6481 cactaagatc aagggatgga ggggaaaatt tcatagtgct tctgcatcta ggaatgaagg
     6541 tggaaattca gaaagttcac tgaaaaatcg ttctgctttc tgtagtgata agctagatga
     6601 atacttggaa aatgaaggca agctgatgga aacaagcatg ggtttttctt ctaatgctcc
     6661 cacatctcct gtggtgtacc agcttcccac taagagtacc agttatgtac gaacacttga
     6721 tagtgtacta aagaagcaat ctactatttc cccttctacc tcttattctt tgaaacctca
     6781 ttctgtaccc cctgtctctc gaaaggcaaa gtctcaaaac agacaggcaa ctttcagtgg
     6841 ccgaactaaa tcatcttata aatccatttt accataccct gtttcaccaa agcagaaata
     6901 ctctcatgtg attctaggag ataaggttac caagaattct tcaggcatca tctcagaaaa
     6961 tcaggcgaat aactttgttg tgccaacttt ggatgaaaat atatttccaa agcagattag
     7021 tttgcggcag gcacagcagc agcagcaaca gcaacaggga agtcgccctc caggcttgtc
     7081 taaatctcag gtgaagctaa tggacctgga agactgtgca ctttgggaag gaaaaccaag
     7141 gacatacatc acagaagagc gagcagatgt atccttaaca actctactta cagctcaagc
     7201 atccctcaaa actaaaccta tccacacaat cataaggaaa cgagcccctc cctgcaacaa
     7261 tgacttctgt cgactgggtt gtgtatgttc cagtctagct ttggagaagc gccaacctgc
     7321 tcactgccgc cgaccagact gcatgtttgg ttgtacttgt ttgaaaagaa aagttgtact
     7381 tgttaaagga ggatccaaaa ctaagcattt tcagaggaag gctgctcatc gagatccagt
     7441 attttatgat actctgggag aggaggcaag ggaggaggaa gaaggaatca gggaggagga
     7501 ggaacaattg aaagagaaaa agaagagaaa gaagctagaa tacactatat gtgagacaga
     7561 gcctgaacag cctgttcgac attacccatt atgggtaaaa gtagaaggtg aagtagatcc
     7621 agaaccagtt tatatcccca cgccttctgt cattgagcct atgaaaccat tgttattgcc
     7681 tcagccagaa gttttatctc ctactgtgaa gggcaaactg ctcactggaa ttaaatctcc
     7741 acggtcatat actcccaaac ccaatcctgt gattcgggaa gaggacaaag atccagtcta
     7801 cttgtacttt gaaagtatga tgacttgtgc tcgagttcga gtatatgagc gaaaaaaaga
     7861 ggaccagaga caaccatctt cctcctcctc cccatctcca tcatttcagc agcaaacttc
     7921 atgtcattct agccctgaga accataataa tgcaaaggaa cctgattctg aacagcagcc
     7981 cttaaaacaa ctcacctgtg acttggagga tgattctgat aaattacaag aaaagagctg
     8041 gaagtcttcc tgcaatgaag gagaatcctc ttctacttct tatatgcatc agaggtcacc
     8101 tggtggtccc accaaactga ttgagatcat ctcagactgc aactgggagg aagatcggaa
     8161 caagattttg agcatcttat cccagcacat caatagcaac atgccacaat cacttaaggt
     8221 gggcagcttc atcattgagt tggcttctca gcgaaagagc cggggtgaga agaaccctcc
     8281 tgtttattct tctcgtgtga aaatctctat gccatcatgt caagaccaag atgatatggc
     8341 tgagaaatct ggatcagaga ctcctgatgg tccattgtcc cctgggaaaa tggaggatat
     8401 ctctcctgtg cagacagatg ccctggattc agtgagggag agattacatg gaggcaaagg
     8461 tctgcctttt tatgcagggc tttctcctgc agggaagctt gtggcctata aacgtaaacc
     8521 cagttcaagt acatctgggc ttatccaggt agcatccaat gccaaggtgg ctgcatccag
     8581 gaaaccacgt accctgttgc cttcaacatc caattccaaa atggcatcct cctctggcac
     8641 tgcaacaaat cgccctggga agaatctgaa ggcgtttgtc ccagcaaaac ggccaattgc
     8701 ggctcgaccc tctcctggtg gtgtgttcac acagtttgtg atgagtaaag ttggagcctt
     8761 gcagcagaag atacctggag ttagcacacc ccaaaccctg gcagggacac agaagttcag
     8821 tatcagacct tctccagtaa tggtcgtcac acctgtggtt tcttctgagc cagttcaggt
     8881 gtgcagccct gtgactgctg ctgtcactac taccacccct caagtgtttt tagaaaatac
     8941 tactgctgtg acacctatga ctgctatttc tgacgtggaa actaaagaaa ctacttattc
     9001 ttctggtgcc accactacag gggttgttga ggtctctgaa actaatacca gcacctctgt
     9061 aacatctacc cagtctacag ccactgtgaa ccttaccaaa accactggga taactacccc
     9121 tgtggcttca gttgcttttc ctaagtcttt ggtagcatct ccttcaacca taactcttcc
     9181 tgttgcttcc actgcttcca cctccttagt cgtggtgact gcagctgcat cttcctccat
     9241 ggtgaccaca ccaacttcat ctctgggctc tgttcctatt atactctcag gaattaatgg
     9301 gagtccacca gtgagccaga gaccagaaaa tgctgctcaa attccagtgg ctactccaca
     9361 ggtctctcct aacacagtga aacgtgctgg acctcgattg ttgttgattc cagtgcagca
     9421 gggttctcct actcttagac ctgtctcaaa cacacaactt cagggacatc ggatggtctt
     9481 gcagcctgtt aggagtccaa gtggaatgaa cttattcagg caccctaatg ggcagattgt
     9541 ccagcttcta cctttgcatc agcttcgagg ctctaatacc cagcccaact tacagcctgt
     9601 catgtttcgg aacccagggt ctgtgatggg aatccggtta cctgctcctt ccaaaccctc
     9661 tgagactccg ccatcttcca cttcgtcctc tgctttctct gtcatgaatc ctgtaattca
     9721 agctgttggg tcttcttcag cagtgaatgt tatcactcag gcaccatcat tgctttcctc
     9781 tggagctagt tttgtgtctc aggctggtac attgaccctg aggatttctc ctcctgaacc
     9841 acaaagcttt gcaagtaaaa caggctctga aaccaaaata acttatagct caggaggaca
     9901 gcctgttggt acagccagtc ttattcctct ccagtctggt agttttgcct tgttacagct
     9961 cccaggacaa aagcctgttc ctagctccat tcttcagcat gttgcttccc ttcagatgaa
    10021 gagagaatct cagaatccag accagaaaga tgaaacaaac tcaataaaaa gagagcaaga
    10081 aacgaagaag gttctacagt cagaaggaga ggctgtagac cctgaggcta atgtaataaa
    10141 acaaaactca ggagctgcta cctcagaaga aactctgaat gattccttgg aagatagggg
    10201 tgatcatttg gatgaagaat gccttccaga agaaggttgt gcaactgtca aaccatctga
    10261 gcattcctgt atcactgggt cacatacaga tcaagattat aaagatgtta atgaagaata
    10321 tggggctagg aatcgtaaga gttccaaaga aaaagtggct gttctggaag ttaggaccat
    10381 ttctgaaaaa gccagtaata agacagtcca aaatttaagt aaagtacagc atcaaaaact
    10441 tggtgatgtg aaggtggaac agcagaaagg atttgacaat ccagaagaaa actcaagtga
    10501 atttccagtc acctttaagg aagaaagtaa atttgaattg tcaggaagca aagttatgga
    10561 gcagcaatct aatctacagc cagaggccaa agagaaggaa tgtggagact ctctggagaa
    10621 agacagggaa agatggagaa aacatctgaa gggcccctta accaggaaat gtgttggagc
    10681 ttcacaggaa tgtaagaaag aggcagacga gcagttaatt aaagaaacaa agacatgtca
    10741 ggaaaattca gatgtgtttc agcaagaaca aggcatctct gacttacttg gaaaaagtgg
    10801 aattactgaa gatgccagag ttttgaaaac tgaatgtgat tcttggagta ggatttctaa
    10861 tccttcagcc ttctccattg ttcctaggag agctgcaaaa agcagcagag ggaatggaca
    10921 ttttcagggt cacttactgc tacctggaga acagatacaa ccaaagcaag agaagaaggg
    10981 tgggagaagc agtgctgact tcactgtttt ggatttggaa gaagatgatg aagatgataa
    11041 tgagaaaact gatgattcta ttgatgaaat tgtggatgtt gtttctgact accagagtga
    11101 ggaggttgat gatgtagaaa agaataactg tgtagaatac attgaggatg atgaggagca
    11161 cgtggacatt gagactgtag aagagctctc agaggaaatt aatgttgctc acctgaagac
    11221 cacagcggcc cacacacagt cattcaaaca gccgtcctgt actcacatct ctgcagatga
    11281 aaaagcagct gaaaggagtc gaaaggctcc accaattcct ctaaaactga agcctgatta
    11341 ctggagtgac aaactacaga aagaagcaga agcgtttgct tattatcgcc ggacacacac
    11401 tgccaatgag cggcggcggc gtggtgaaat gagggatctc tttgagaaat taaagatcac
    11461 attgggatta cttcattctt ccaaggtttc caaaagtctc attcttactc gagccttcag
    11521 tgaaattcag ggactaacag atcaggcaga caaattgata ggacagaaaa atctcctgac
    11581 tcgaaaacgg aatattctga tacggaaagt atcgtctctt tcaggtaaga cagaagaagt
    11641 ggtcctgaag aagctagagt atatttatgc aaaacagcaa gcactagagg cacaaaaaag
    11701 aaaaaagaag atgggatcag atgagtttga catatctccc agaattagca aacagcagga
    11761 aggatcttct gcatcatctg tagatcttgg acagatgttt ataaataaca ggagggggaa
    11821 acctttgatt ctttccagaa aaaaagacca ggccacagaa aatacctcac ccttgaacac
    11881 tccacacacc tctgccaacc ttgtgatgac tccgcaaggg caattgctca ccctaaaagg
    11941 tcccctattc tcaggaccag tggtagctgt ttctcctgat ctcttagaat ctgatcttaa
    12001 gcctcaagtt gccggtagtg ctgtggctct accagaaaat gacgacttat ttatgatgcc
    12061 acgaattgtt aatgtgacat cattggccac agagggaggt ttggtagata tgggtggcag
    12121 caaatatcct catgaagttc ctgatagcaa gccatctgac catctgaaag acaccgtcag
    12181 gaatgaagat aattccttag aggataaggg tagaatctct tccagaggaa acagagatgg
    12241 cagagtgacg ttgggtccaa cgcaggtttt tctggcaaac aaagattctg gttatccaca
    12301 aatagttgac gtttccaata tgcagaaagc acaagagttc ttacctaaaa agatttctgg
    12361 tgatatgaga gggattcagt ataaatggaa agagagtgaa tcaagagggg agagagtgaa
    12421 gtcaaaggat tcttcatttc ataaattaaa gatgaaagat ctcaaggact caagcataga
    12481 gatggaactg aggaaagtaa catcagctat agaggaagca gctcttgatt ccagtgaact
    12541 gctgactaac atggaagatg aggatgatac tgatgagaca ctgacttcac tgctcaatga
    12601 aattgccttt cttaatcaac aactaaatga tgactctgtt ggcctggctg aactacccag
    12661 ctctatggat acagagttcc caggggatgc tcggcgggct tttattagta aggttcctcc
    12721 tggaagcaga gcaactttcc aggttgagca cttgggaact ggtttgaaag agttgcctga
    12781 tgttcaaggg gagagtgact ctatcagtcc cctcctcttg cacttggaag acgatgactt
    12841 ttctgagaat gaaaaacaac ttgcagaacc agcctctgag ccagatgtcc ttaagattgt
    12901 tattgactct gaaataaagg attccctcct ttccaacaag aaagctattg atggagggaa
    12961 gaatacttct ggcctccctg cagagcccga aagtgtgtcc tcacccccca ccctacacat
    13021 gaagactggc ttggagaaca gcaacagcac agacactttg tggaggccta tgccaaagtt
    13081 ggcccctcta ggtttaaaag tagctaatcc ttccagtgat gcagatggtc agagtctcaa
    13141 ggtgatgcct tgtttggcac ctatagctgc caaagttggg tcagttggac acaaaatgaa
    13201 cttaacaggg aatgaccagg aaggccggga aagcaaggtg atgcctacat tggcacctgt
    13261 tgtggctaaa ttgggcaact cgggggcctc accaagttct gcagggaaat agtaatgaac
    13321 tagtccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
    13381 tgttgcgtat ggagttaaga gtacacgcgt taagtcgaca atcaacctct ggattacaaa
    13441 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
    13501 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
    13561 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
    13621 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
    13681 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
    13741 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
    13801 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
    13861 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
    13921 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
    13981 cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag
    14041 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
    14101 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
    14161 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
    14221 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
    14281 gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta
    14341 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
    14401 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
    14461 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
    14521 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
    14581 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
    14641 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
    14701 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
    14761 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
    14821 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
    14881 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
    14941 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
    15001 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
    15061 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
    15121 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
    15181 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
    15241 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
    15301 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
    15361 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
    15421 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
    15481 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
    15541 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
    15601 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
    15661 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
    15721 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
    15781 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
    15841 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
    15901 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
    15961 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
    16021 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
    16081 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
    16141 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
    16201 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
    16261 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
    16321 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
    16381 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
    16441 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    16501 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    16561 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    16621 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
    16681 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    16741 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    16801 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    16861 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    16921 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    16981 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    17041 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    17101 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    17161 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    17221 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    17281 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
    17341 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
    17401 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
    17461 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
    17521 caattaaccc tcactaaagg gaacaaaagc tggagctgca agc
//
